Gnotobiotic IL-10; NF-κB Mice Develop Rapid and Severe Colitis Following Campylobacter jejuni Infection

Department of Medicine and Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
PLoS ONE (Impact Factor: 3.23). 10/2009; 4(10):e7413. DOI: 10.1371/journal.pone.0007413
Source: PubMed


Limited information is available on the molecular mechanisms associated with Campylobacter jejuni (C. jejuni) induced food-borne diarrheal illnesses. In this study, we investigated the function of TLR/NF-kappaB signaling in C. jejuni induced pathogenesis using gnotobiotic IL-10(-/-); NF-kappaB(EGFP) mice. In vitro analysis showed that C. jejuni induced IkappaB phosphorylation, followed by enhanced NF-kappaB transcriptional activity and increased IL-6, MIP-2alpha and NOD2 mRNA accumulation in infected-mouse colonic epithelial cells CMT93. Importantly, these events were blocked by molecular delivery of an IkappaB inhibitor (Ad5IkappaBAA). NF-kappaB signalling was also important for C.jejuni-induced cytokine gene expression in bone marrow-derived dendritic cells. Importantly, C. jejuni associated IL-10(-/-); NF-kappaB(EGFP) mice developed mild (day 5) and severe (day 14) ulcerating colonic inflammation and bloody diarrhea as assessed by colonoscopy and histological analysis. Macroscopic analysis showed elevated EGFP expression indicating NF-kappaB activation throughout the colon of C. jejuni associated IL-10(-/-); NF-kappaB(EGFP) mice, while fluorescence microscopy revealed EGFP positive cells to be exclusively located in lamina propria mononuclear cells. Pharmacological NF-kappaB inhibition using Bay 11-7085 did not ameliorate C. jejuni induced colonic inflammation. Our findings indicate that C. jejuni induces rapid and severe intestinal inflammation in a susceptible host that correlates with enhanced NF-kappaB activity from lamina propria immune cells.

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    • "The development of acute disease caused by C. jejuni has been studied in multiple animal models, however the IL-10−/− germ-free mouse model has emerged as a reproducible and reliable model system for the study of campylobacteriosis.39,40 We used bacterial invasion of the spleen as an indicator of C. jejuni disease.41,42 "
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    • "Whereas Mansfield and colleagues demonstrated that enteritis developed after more than 28 days upon C. jejuni infection in IL-10−/− mice housed under specific pathogen free conditions (and depending on their genetic background) [37], [38], in our study, C. jejuni induced a rapid ulcerative colitis in gnotobiotic IL-10−/− mice. Using germfree IL-10−/− NF-κBEGFP mice raised under isolator-conditions, Lippert et al. highlighted the role of NF-κB in C. jejuni induced pathogenesis [39]. Following infection, germfree IL-10−/− NF-κBEGFP mice displayed mild symptoms at day 5 and ulcerative colitis at day 14 following C. jejuni strain 81–176 infection. "
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