A new resin-bonded retrograde filling material

Department of Conservative Dentistry, Asan Medical Center, Ulsan University, Seoul, Korea.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology (Impact Factor: 1.46). 11/2009; 108(5):e111-6. DOI: 10.1016/j.tripleo.2009.07.020
Source: PubMed


This study determined the physical properties and cytotoxicity of a novel root-end filling material (NRC).
NRC is a powder and liquid system. The liquid is composed of hydroxyethylmethacrylate, benzoyl peroxide, toluidine, and toluenesulfinate. And the powder is made of calcium oxide, calcium silicate, and triphenylbismuth carbonate. The setting time, compressive strength, and pH change of NRC and gray and white mineral trioxide aggregate (MTA) were determined according to ISO standardization. MC3T3-E1 cells were cultured on NRC and white MTA for determining MTT scores. The absorbance of formazan was measured at 570 nm with a spectrophotometer. The MTT assay was performed in triplicate and repeated in 2 cultures. One-way analysis of variance was used to determine statistical differences in physical properties and MTT assay (P < .05).
Mean setting time of materials tested were: NRC 12.5 +/- 0.3 minutes, gray MTA 345.5 +/- 96.2 minutes, and white MTA 318.0 +/- 56.0 minutes. After 24 hours, the mean compressive strengths were: NRC, 21.6 +/- 5.5 MPa, gray MTA: 7.7 +/- 3.3 MPa, and white MTA, 18.9 +/- 3.2 MPa. The pH of the test materials were: NRC 12.0, gray MTA 12.2, and white MTA 11.9. There were no statistically significant differences in compressive strength and pH between white MTA and NRC. The compressive strength of gray MTA was significantly lower than white MTA and NRC (P < .05). The setting time of NRC was significantly lower than white and gray MTA. In MTT assay, both NRC and white MTA were not cytotoxic to MC3T3-E1 cells.
It was concluded that the setting time, compressive strength, pH, and initial biocompatibility results of NRC are favorable for a root-end filling material.

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    • "It contains bioactive powders such as calcium oxide and calcium silicate, triphenylbismuth carbonate as opacifier. Organic resin matrix of this cement is composed of 2-hydroxyethylmethacrylate (HEMA) as a resin monomer, benzoyl peroxide as an initiator, toluidine and toluenesulfinate as catalyst and accelerator.7 The probable polymerization reaction of NRC is shown at Figure 1. "
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    ABSTRACT: This study investigated the cytotoxicity of bioaggregate (BA) and the effect of BA on mineral associated gene expression in osteoblast cells. The cytotoxicity of BA to mouse MC3T3-E1 osteoblast cells was evaluated via the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 1, 2, and 3 days of culture. The expression of mineral associated genes (collagen type 1, osteocalcin, and osteopontin) was assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and compared expression after exposure to BA or mineral trioxide aggregate (MTA). The data were analyzed by one-way analysis of variance and the Tukey test. BA was essentially nontoxic to osteoblast cells. The expression of collagen type 1, osteocalcin, and osteopontin genes significantly increased in the BA group compared with that in the MTA group on the second or third day of culture. BA appears to be a novel nontoxic root-end filling biomaterial and be able to induce mineralization-associated gene expression in osteoblast cells.
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