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FORMULATION OF SOLID PERFUME FROM LOCALLY AVAILABLE CHRYSANTHEMUM X MORIFOLIUM (GARDEN CHRYSANTHEMUM) AND THEOBROMA OIL

Authors:

Abstract

Chrysanthemums have a vast supply of cut flowers but limited use in the farming industry. This is a serious issue that every farmer experiences during harvest. As a result, a formulation of solid perfume from garden chrysanthemums may provide a remedy for this issue. This research aims to create a solid perfume from locally accessible Chrysanthemum x morifolium (Garden Chrysanthemum) and Theobroma oil. The researchers employed Rotary Evaporation to extract essential oils from Garden Chrysanthemum petals and formulate a solid perfume from cocoa butter, beeswax, avocado, and Garden Chrysanthemum oil at the University of Baguio. The formulated solid perfumes underwent tests for their homogenous, hardness/strength, pH, and organoleptic properties. Where the results showed that the three formulations were homogenous, the different concentrations affected the physical appearance of the solid perfume.
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DOI 10.35631/IJIREV.616008
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INTERNATIONAL JOURNAL OF
INNOVATION AND
INDUSTRIAL REVOLUTION
(IJIREV)
www.ijirev.com
FORMULATION OF SOLID PERFUME FROM LOCALLY
AVAILABLE CHRYSANTHEMUM X MORIFOLIUM (GARDEN
CHRYSANTHEMUM) AND THEOBROMA OIL
Rochele Yao S. Benan1*, Shariya Mei L. Pedro2, Rimson George A. Ba-oy3, Trisha Mhae L. Pilay4,
Christian Yoshua D. Sacramento5, Benjamin A. Litusquen6, Honey Khylle W. Dimatulac7, Aubrae
Frances E. Bannawi8
1
Saint Louis School of Pacdal, Inc.
Email: slspi.rocheleyaobenan@gmail.com
2
Saint Louis School of Pacdal, Inc.
Email: slspi.shariyameipedro@gmail.com
3
4
5
6
7
8
Saint Louis School of Pacdal, Inc.
Email: slspi.rimsongeorgebaoy@gmail.com
Saint Louis School of Pacdal, Inc.
Email: slspi.trishamhaep@gmail.com
Saint Louis School of Pacdal, Inc.
Email: slspi.christianyoshuasacramento@gmail.com
Saint Louis School of Pacdal, Inc.
Email: slspi.benjaminjrlitusquen@gmail.com
Saint Louis School of Pacdal, Inc.
Email: slspi.honeykhylledimatulac@gmail.com
Saint Louis School of Pacdal, Inc.
Email: aubraefrancesb@gmail.com
*
Corresponding Author
Article Info:
Article history:
Received date:14.12.2023
Revised date: 31.01.2024
Accepted date: 25.02.2024
Published date: 12.03.2024
To cite this document:
Benan, R. Y. S., Pedro, S. M. L., Ba-
oy, R. G. A., Pilay, T. M. L.,
Sacramento, C. Y. D., Litusquen, B.
A., Dimatulac, H. K. W., & Bannawi,
A. F. E. (2024). Formulation of Solid
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DOI 10.35631/IJIREV.616008
Copyright © GLOBAL ACADEMIC EXCELLENCE (M) SDN BHD - All rights reserved 111
Perfume from Locally Available
Chrysanthemum X morifolium
(Garden Chrysanthemum) and
Theobroma Oil. International Journal
of Innovation and Industrial
Revolution, 6 (16), 110-123.
DOI: 10.35631/ IJIREV.616008
This work is licensed under CC BY 4.0
Introduction
Solid perfumes, cream perfumes, or solid colognes are perfumes that are solid rather than the
liquid mix of alcohol and water or fragrance and carrier oil that is used in most perfume
applications (Mroczek, 2022). According to Jones (2021), solid perfumes are typically made
with natural wax, such as beeswax or shea butter, as the fragrance medium instead of water.
After that, the product is gently rubbed into the skin, usually at pulse points like the neck or
wrist. Seow (2021) stated that several high-end perfume brands offer their characteristic aromas
in solid perfume formulations. Unlike regular perfumes and colognes, solid fragrances are
compact and leak-proof because of their waxy substance. Additionally, since solid fragrances
do not leave a spillage, consumers may easily reapply in public without being seen.
Solid perfumes with essential oils are gaining appeal as a natural and sustainable alternative to
standard liquid perfumes (Shinde et al., 2023). According to Debadatta et al. (2024), the global
solid perfume market was valued at USD 1.65 billion in 2023 and is expected to reach USD
4.7 billion by 2032, growing at an 11.1% compound annual growth rate from 2024 to 2032.
The growing consumer interest in personal care and fragrance goods drives growth in the global
solid perfume industry. Cosmetics and perfumery technological advancements have altered
customer perceptions and use of fragrance goods. These scents, praised for their simplicity and
mobility, represent a creative innovation in the fragrance industry, attracting widespread
attention.
Furthermore, Chrysanthemum morifolium is one of the world's four great cut flowers, with
significant ornamental and commercial value. Flower fragrance is a significant ornamental
characteristic of chrysanthemum, particularly for tea and edible chrysanthemum, and the
quality of the scent influences its commercial value (Wang et al., 2023). Chrysanthemums have
long been used in the perfume industry; many perfumes are derived from these flowers, such
as Serge Lutens' De Profundis, a Floral fragrance for men and women. De Profundis debuted
in 2011. Christopher Sheldrak is the nose behind this fragrance (Fragrantica, 2022).
Furthermore, Amouage also released a perfume in 2016 called Myths Woman, a seductive
floral-green fragrance for ladies. This smell-artistic creation was created by the well-known
perfumer Nathalie Lorson (CrisMS, 2024).
Cocoa butter (theobroma oil) is a pale-yellow, edible vegetable fat derived from cocoa beans
with a mild chocolate flavor and aroma. It is used to produce chocolate confections,
pharmaceutical ointments, and toiletries. It is valued for its melting properties, as it remains
brittle at room temperature or lower but melts just below body temperature (Rogers, 2021).
According to Zelman (2020), cocoa butter is an excellent choice due to its high fatty acid
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content as the main component of skin creams. Fatty acids aid skin hydration. The fat in cocoa
butter forms a barrier that keeps moisture in and stops the skin from drying out. Cocoa butter
is also high in phytochemicals, which are natural plant compounds. These substances may
improve blood flow to the skin and slow skin aging by protecting against UV ray damage.
Cocoa butter smooths scars, wrinkles, and other skin imperfections. Many women believe that
cocoa butter creams and lotions can be used to prevent and minimize the appearance of stretch
marks during and after pregnancy. Cocoa butter has also been promoted for its ability to heal
rashes caused by conditions such as eczema and dermatitis (Watson, 2017).
A previous study was conducted by Septiyanti (2021), which used jasmine oil. The results
showed that the most preferred formulation based on the organoleptic test was produced with
a 30% cocoa butter concentration with a preference level of 3.63. Solid perfume with 30%
cocoa butter concentration had characteristics of a penetration level of 14.16 mm/sec, melting
point of 90° C, pH value of 4, and IC50 201.98 μg/mL. Solid perfume products showed
homogeneity and physical stability during four weeks of observation at room temperature.
As a result, the researchers will focus on using Chrysanthemum x morifolium oil extract as a
raw material for this study. Correspondingly, in this study, the formulation of solid perfume
will be optimized by using cocoa butter as the raw material and Chrysanthemum x morifolium
(Garden Chrysanthemum). The solid form aims for ease of use and storage while increasing
stability. This study will characterize solid perfume products, including an organoleptic test,
homogeneity test, strength/hardness level test, and pH test.
Literature Review
Solid Perfume
Solid fragrance is a "new" product that is quite old. It dates back to ancient Egypt but has
recently gained popularity. Solid perfumes, cream perfumes, or solid colognes are solid rather
than the liquid mix of alcohol and water or fragrance and carrier oil used in a typical perfume
application (Mroczek, 2022). According to Vicki (2023) and Mroczek (2022), solid perfumes
are scents available in a solid form, usually in the shape of a paste or balm. The aroma lasts
longer on the skin because of a combination of waxes, oils, and other chemicals used in its
creation than conventional liquid perfumes. To name a few benefits of solid perfume, solid
perfumes are alcohol-free (Singh, 2019; Connett, n.d.; Srivastava, 2021), almost zero waste
(Amanvida, 2021; Connett, 2019), moisturizing, and nourishing (Singh, 2019; Nuutjob, 2023;
Cobes, 2022). Additionally, solidified perfumes eliminate the possibility of spilling or leaking
due to a crack in the perfume container (Singh, 2019). A handy perfume alternative is a solid
perfume. Because of its creamy smoothness, this type of scent is portable (Slesarenok &
Yakovleva, 2019). Moreover, there are various body parts to which you can apply solid
perfume, such as the wrist, neck, pulse point, and even your hair (Mroczek, 2022). Solid
perfumes are usually applied by rubbing a small amount on the body's pulse spots, such as the
wrists or neck, using a finger or a cotton swab. The body's heat helps release the fragrance over
time, and the perfume's solid consistency helps extend the scent's longevity (Pal et al., 2014 &
Shinde et al., 2023). Cocoa butter is made from the cocoa bean, the seed of the Theobroma
tree, primarily cultivated in South America and West Africa (Naik et al.; V., 2014). Reinhardt
(2022) stated that cocoa butter has a pale yellow hue and a strong cocoa flavor and aroma.
Despite the name, this fat is entirely vegan and plant-based, derived solely from the cacao bean.
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Theobroma Oil/Cocoa Butter
Theobroma oil, or cocoa butter, is most commonly found in body and face lotions. It is,
however, used in cooking, in both sweet and savory dishes. Cocoa butter has a cocoa flavor
and aroma and is classified as a vegetable fat. Despite the name's use of the word "butter," it is
vegan and contains no dairy products (Murray, 2022). Cocoa butter is used as a raw material
in many cosmetic products, including cream, sunscreen, lipstick, soap, and shampoo (Ristanti
et al., 2018). Cocoa butter is high in vitamin D2, a precursor to the body's active form of vitamin
D. It is also high in minerals like potassium, phosphorus, iron, copper, and magnesium but low
in health-promoting polyphenols (Alexis, 2022).
Cocoa butter is a rich emollient that moisturizes and nourishes even the most parched skin
(think chapped hands or lips). It is solid at room temperature and melts nicely when applied. It
contains fatty acids, primarily oleic (35%), stearic (34%), and palmitic (25%) fatty acids, as
well as antioxidant vitamin E and polyphenols (Gasser et al., 2008 & SuperZero, 2022).
Chrysanthemum X morifolium (Garden Chrysanthemum)
Linnaeus coined the term "chrysanthemum" in 1753, combining the Greek words "chrysos"
(gold) and "anthemom" (flower) to describe the golden flowers of the first chrysanthemum
varieties (Boase et al., 2004). Although a wide variety of flower colors, shapes, and forms were
initially developed through conventional and mutant breeding, transgenic procedures are now
more commonly used, with Agrobacterium-mediated transformation being the most prevalent
method of introducing foreign genes into chrysanthemums (Da Silva et al., 2013). According
to Sivers (2022), Chrysanthemums were first documented as a flowering herb in China in the
15th century B.C. The earliest illustrations of chrysanthemums (around the 17th century A.D.)
described them with small yellow flowers, even though chrysanthemums had been depicted on
ancient Chinese pottery hundreds of years prior. Chrysanthemum oil and extract have also been
used in herbal medicine for their antibacterial and antibiotic properties. According to Gaddis
(2022), essential oil extracted from the chrysanthemum plant has long been used as an all-
natural organic pesticide and insect repellent. The oil of the chrysanthemum flower has a
pleasant scent; because of their pleasant fragrance, the dried petals of the chrysanthemum
flower have been used in potpourri and to freshen linens for hundreds of years. Chrysanthemum
oil can also be used in perfumes or scented candles. The scent is light and flowery without
being heavy.
Methodology
Research Design
The researchers adopted a true experimental type of Quantitative Research design to extract oil
and formulate a perfume from locally available Chrysanthemum. Bhandari (2022) defined
quantitative research as the process of collecting and analyzing numerical data. It can find
patterns and averages, make predictions, test causal relationships, and generalize results to
broader populations. According to Gautam (2017), true experimental research relies on
statistical analysis to prove or disprove a hypothesis, making it the most accurate form of
research. Out of the types of experimental design, only true design can establish a cause-effect
relationship within a group. In a true experiment, three factors need to be satisfied: there is a
control group, which will not be subjected to changes, and an experimental group, which will
experience the changed variables; a variable that the researcher can manipulate; and a random
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distribution. The research design aims to evaluate the efficacy of using a Chrysanthemum as
an alternative ingredient in perfume formulation.
Methods and Materials
The materials required to characterize scent during the formulation of perfume materials
include some of the listings below, such as Tripod, Gauze Pad, Beakers, Stirrer, and Denatured
Alcohol. They enable the division of the numerous parts. All of the materials in this project
were of natural origin. Furthermore, the main components needed for a solid perfume are as
follows with their respective prices: Beeswax (250g, Php 248), Avocado Oil (100g, Php 110),
Chrysanthemum X morifolium, and Cocoa butter (250g, Php 238). Furthermore, the
researchers picked about 5 kg of Garden Chrysanthemum and removed their petals. The flower
petals were placed under the sun as the process of Rotary Evaporation requires dried and crisp
petals; about 213.64 grams of dried petals were collected. The researchers were asked to blend
the dried petals until they became powdery. The blended petals were soaked in 1 Liter of
ethanol for 48 hours. After 48 hours, the researchers filtered the solution. Lastly, the university
performed the process of rotary evaporation.
Garden Chrysanthemum Rotary Evaporation
Chrysanthemum x morifolium essential oil is obtained by rotary evaporation, wherein the
distillation of Garden Chrysanthemum produces a yellow oil. This follows the research of Jang
et al. (2021) and the study of Liu et al. (2017) that extracted essential oil from the same species,
resulting in yellow-colored oil with a distinctive flower aroma. 0.78% of essential oil is
obtained using the process of rotary evaporation from the 5 kg of Chrysanthemum flowers. The
essential oil yielded 39 mL.
Solid Perfume Formulation
In this study, the preparation used a solid and liquid base combination, cocoa butter, beeswax,
avocado oil, and the essential oil of Chrysanthemum flowers. This research used cocoa butter
and beeswax as a base. Cocoa butter concentrations used were 5g (10%) for F1, 10g (20%) for
F2, and 15g (30%) for F3. Formula 3 has the highest amount of cocoa butter, followed by
Formula 2, and Formula 1 has the least amount of cocoa butter. According to Zelman (2020),
cocoa butter is an excellent choice due to its high fatty acid content as the main component of
skin creams. Fatty acids aid skin hydration. The fat in cocoa butter forms a barrier that keeps
moisture in and stops the skin from drying out. Beeswax was used with concentrations of 50%
(F1), 40% (F2), and 30% (F3). Formula 1 has the most beeswax, 25g of beeswax, followed by
Formula 2, which has 20g of beeswax, and lastly, Formula 3 has the least amount of beeswax,
15g. Beeswax was chosen because it can increase the consistency of the preparation and
dissolve in essential oils (Maysarah et al., 2020).
The researchers melted the cocoa butter at 90°C. Garden Chrysanthemum oil as a fragrance
was mixed with avocado oil and then added to the wax compound. The mixture was stirred
until homogeneous, then cooled and hardened at room temperature (Septiyanti et al., 2021).
The composition of a solid perfume is shown below:
Table 1: Composition Of Solid Perfume
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Variables
F1
F2
F3
Cocoa Butter
5g
10g
15g
Beeswax
25g
20g
15g
Avocado Oil
10g
5g
5g
Chrysanthemum Oil
10g
15g
15g
Solid Perfume Evaluation
The formulation of solid perfume and the necessary tests, such as the Organoleptic Test,
Melting Temperature Test, Hardness Level Strength/Strength Test, pH Test, and Homogeneity
Test, were adopted from the study of Maysarah et al. (2020).
Organoleptic Test
Stick perfume preparations were observed for several parameters, such as color, consistency,
and aroma. (Hernani, 2012 cited by Maysarah, et al., 2020). The organoleptic test is a method
of testing using the five human senses (Septiyanti et al., 2021).
Hardness Level Test
The solid perfume was placed horizontally and about 1.5 cm from the edge of the tin can; a
load was hung to give pressure. Every 30 seconds, 10 grams was added until the preparation
was broken. (Nazlinawaty et al., 2012 cited by Maysarah, 2020).
pH Test
The pH level of the formulated solid perfume will be tested by the researchers using a pH meter
(Septiyanti et al., 2021). It is equipment that detects the activity of hydrogen ions in solutions
or the acidity or alkalinity of a solution. In the end, the pH level, which typically ranges from
1 to 14, is used to describe the level of hydrogen ion activity. The pH scale measures acidity,
with 0 being very acidic and 14 being very alkaline (Iowa State University, 2023 ).
This pH measurement is directly related to the ratio of hydrogen ion concentration and
hydroxyl ion concentration ([H+] and [OH-], respectively). The general breakdown of pH
levels is as follows: Neutral solution: pH = 7, Acidic solution: pH < 7, and Basic solution: pH
> 7
Homogeneity Test
A homogeneity test of solid perfume will be conducted by applying the samples to a flat glass.
According to Mappa et al. (2013), as cited by Maysarah et al. (2020), 0.5 g of stick perfume
was taken, applied to the glass preparation, and covered with glass. Then, the naked eye was
used to observe the presence of coarse grains.
Data Analysis
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By the time the researchers had completed formulating the solid perfume from Garden
Chrysanthemums and recorded all the relevant data, The researchers were able to determine
that 39 mL of essential oil was obtained from 5 kg of the Chrysanthemums, which is 0.78%
through the use of a math equation. To calculate the percentage of a number, divide it by the
whole and multiply by 100, which follows:
(Value ÷ Total value) × 100
After conducting the strength test of the formulated perfumes to get the mean, the researchers
divided the sum of all values in a data set by the number of values.
Mean (x) = Σx/n
Σx = sum of all values
n = number of values
Results and Discussion
Appearance of Solid Perfume
Table 2: Appearance Of The Solid Perfume Preparation
Appearance
Formula
Color
Consistency
Odor
F1
Cream White
Solid
Chrysanthemum
F2
Cream White
Solid
Wax(Cocoa Butter)
F3
Cream White
(slightly darker)
Solid
Wax and Chrysanthemum
Observation of the organoleptic test of color, aroma, and consistency of stick perfume
preparations can be seen in Table 2. According to Lamusu et al. (2012), as cited by Maysarah
et al. (2020), an organoleptic test was carried out to determine the level of liking and
acceptability of the color, taste, aroma, and consistency of the preparation. The organoleptic
test revealed that F1 has a cream-white color and a solid consistency, and its prominent odor is
that of a chrysanthemum. Meanwhile, F2 also has a creamy white color, a solid consistency,
and a prominent smell of the base used, cocoa butter. Lastly, F3 has a slightly darker cream-
white color, a solid consistency, and a mixed aroma of cocoa butter and Chrysanthemum. The
Organoleptic evaluation revealed the details regarding the color, odor, appearance, texture, etc,
of the prepared formulation, which was pleasant, smooth, and acceptable (Septiyanti et al.,
2021).
Table 3: Homogeneity Test Result
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Formula
Homogeneity
F1
Homogenous
F2
Homogenous
F3
Homogenous
To avoid discomfort, a preparation must be homogenous and flat (Naibaho, 2013). The
homogeneity test findings revealed that when all stick perfume compositions were put into
transparent glass, there were no coarse grains. The homogeneity test revealed that F1, F2, and
F3 are homogenous. This demonstrated that the preparations were made consistently (MOH
RI, 1979, as cited by Maysarah et al.,2020 ). Because the ingredients used are soluble in
essential oils, all homogeneous preparations indicated that all formula ingredients were well
mixed, as there were no lumps or coarse grains in the preparations.
Table 4: Strength Test Result
Formula
Mean (g) ± SD
F1
181.86 g
F2
120.11 g
F3
154.56 g
Table 4 discusses the strength test result. The strength test was performed to determine the
strength of the preparation during the packaging, transportation, and storage processes
(Risnawati et al., 2012). The results of the stick perfume strength test showed that F1 has the
highest strength (181.86 g), followed by F3 (154.56), and lastly, F2 has the lowest strength
(120.11). This finding was caused by the formula's varied concentrations of beeswax and cocoa
butter (Sampebarra et al., 2016; Baldino & Gabriele, 2010). To the study of Sampebarra., et al.
(2016) and Anisa et al. (2019), beeswax can enhance the number of solids in the preparation,
resulting in a harder-formed shape. On the other hand, the inclusion of cocoa butter softened
the solid perfume product. The presence of cocoa butter in the formulation was a crucial
component in adjusting the texture and melting point (Septiyanti., et al., 2021; Baldino &
Gabriele, 2010)
Table 5: pH Test Result
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Formula
pH
pH Level
Basic/Acid/N
eutral
F1
7.11
Neutral
F2
7.93
Basic
F3
7.28
Neutral
Table 5 showcases the result of the pH test. pH standard for topical preparation in contact with
skin was about 4-8 (Padmadisastra et al., 2007, as cited by Septiyanti., et al., 2021). The pH
value was expected not to be too acidic because it can irritate and not too alkaline because it
can cause scaly skin (Septiyanti., et al., 2021). The resulting solid perfume was tested using a
pH meter. Formula 1 has a pH level of 7.11, which is neutral; Formula 2 has a pH level of 7.93,
which can be considered essential. Lastly, Formula 3 has a pH level of 7.28, which is neutral.
These pH values are considered safe for topical preparation for human skin application.
According to Ramlah (2017), this solid perfume contained cocoa butter, which contained fatty
acids similar to the fatty acids that are present in the skin. However, the pH level of a person's
skin can also influence how a perfume smells. Due to variances in skin pH levels, a fragrance
may smell differently on one person than another, even if they are wearing the same perfume
(Terry, 2023 & Kessler, 2020).
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Table 6: Overall Results of the Tests
Table 6 is the summary of all the tests conducted for the evaluation of solid perfume. Formula
1 has a prominent scent of Chrysanthemum, a cream-white color, and a solid consistency. The
researchers found that it is homogenous. It has a breaking load of 181.86 g. Lastly, its pH level
is 7. The second formula has a scent of wax (cocoa butter), a solid consistency, and a cream-
white color. It was also homogenous. Its breaking load resulted in 120.11 g, which is basic as
its pH level is 8. The last formulation, formulation 3, has a scent of mixed cocoa butter and
Chrysanthemum; it has a solid consistency and a cream-white color slightly darker than the
previous formulations. It is also homogenous. It has a breaking load of 154.56 g and is neutral,
with a pH value of 7.
Conclusion
Based on the research that has been done, Chrysanthemum x morifolium essential oil can be
formulated into stick perfume preparation. All the tests showed that all formulae can be used
as perfume. The results suggest that Formula 3 is a better formula based on each test parameter.
Formula 3 has a pH level of 7, which means it is neutral and homogenous; it has a strength test
of 154.56 g. Its organoleptic test showed that it has a cream-white color, a solid consistency,
and a mixture of Cocoa Butter and Chrysanthemum as its odor. Furthermore, Formula 3 has a
firm solidity, cream-white tint, chrysanthemum, and wax odor. This is due to the variance in
the formulation. Because of its odor, it can be concluded that it has the best formula. Since
Formula 3 is homogeneous, no coarse granules are visible to the human eye. Because the
formulation is homogeneous, the mixture has the same appearance and qualities. It can be
concluded that wearing perfume prevents irritation. In addition, formula 3 has a strength of
154.56g, as there were no standard strength test values for solid perfumes, yet the researchers
cannot compare and conclude whether the data gathered is excellent. Lastly, formula 3 has a
pH level of 7, which is neutral. It can be concluded that the perfume will not cause any irritation
to the consumers as it has a neutral pH level.
Tests
Organoleptic Test
Homogeneity
Test
Strength
Test
pH Test
Odor
Consistency
Color
Mean (g)
± SD
pH
Leve
l
Basic/Acidic/
Neutral
F1
Chrysanthemum
Solid
Cream
White
Homogenous
181.86 g
7
Neutral
F2
Wax (Cocoa
Butter)
Solid
Cream
White
Homogenous
120.11 g
8
Basic
F3
Wax and
Chrysanthemum
Solid
Cream
White
(slightly
darker)
Homogenous
154.56 g
7
Neutral
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For future studies, other researchers may establish suitable measurements/formulations to
employ in order to improve the quality of the perfume and the mass production of the
chrysanthemum; also, the researchers advise pursuing the method of sun-drying for
chrysanthemum drying and contrasting it with the approach of utilizing an oven to dry the
chrysanthemum to ascertain which method is better. Lastly, a piece of equipment such as a
microscope is used to test the homogeneity of the solid perfume to obtain an accurate result.
Acknowledgment
The researchers would like to extend their utmost appreciation to Mr. Jones Napaldet, a botanist
and a professor from Benguet State University, for his assistance in identifying our plant, to
Ma’am Jacqueline G. Hernandez, CSSP, CSSM, MPH, from the University of Baguio, for
guiding us during the extraction process, and lastly to the faculty and administration of Saint
Louis School of Pacdal, Inc. especially Ms. Danica May M. Petate and Ms. Clydine Jethna O.
Sigue for their tiring and unending support throughout this endeavor.
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Article
Full-text available
Objective The flower of chrysanthemum, used worldwide as a medicinal and edible product, has shown various bioactivities, such as anti-inflammatory, antioxidant, anti-tumorigenic, and hepatoprotective activities, as well as cardiovascular protection. However, the effect of Chrysanthemum morifolium Ramat. on the regulation of osteoclast differentiation has not yet been reported. In this study, we aimed to investigate the inhibitory effect of Chrysanthemum morifolium Ramat. water extract (CME) on RANKL-induced osteoclast differentiation in mouse bone marrow-derived macrophages (BMMs). Study design Bone marrow-derived macrophages (BMMs) isolated from the C57BL/6 J mice. The viability of BMMs was detected with MTT assays. Inhibitory effects of CME on osteoclast differentiation and bone resorption was measured by TRAP staining and Pit assay. Osteoclast differentiation-associated gene expression were assessed by Real-time quantitative polymerase chain reaction. Intracellular signaling molecules was assessed by western blot. Results CME significantly inhibited osteoclast differentiation in BMMs without cytotoxicity, besides inhibiting MAPK/c-fos and PLCγ2/CREB activation. The inhibitory effects of CME on differentiation-related signaling molecules resulted in significant repression of NFATc1 expression, which is a key transcription factor in osteoclast differentiation, fusion, and activation. Conclusion Our results confirmed the inhibition of RANKL-induced PLCγ2/CREB/c-fos/NFATc1 activation by CME during osteoclast differentiation. The findings collectively suggested CME as a traditional therapeutic agent for osteoporosis, RA, and periodontitis.
Conference Paper
Full-text available
Formulation of stick perfume from jeumpa flower’s essential oil (Michelia champaca) has been conducted. Essential oil from jeumpa flowers was obtained by steam distillation method. There were two formulas examined in this study, those are F1 (cera alba 35,07%), F2 (cera alba 40,07%), using 8% concentrations of jeumpa flowers essential oil. Organoleptic, homogeneity, melting point, strength and stability were evaluated as quality parameters of stick perfume. The evaluation results showed that the stick perfume was homogeneous, the melting temperature was 56-59oC, the strength was 343,33 g (F1), F2 and 380 g (F2), respectively. All formulas were stable and did not cause irritation so it safe to use. Hedonic test result showed that F1 is preferred by panelists rather than F2 from all of parameters (shape, fragrance, stickiness, flatness). Based on the results of the quality evaluation it can be concluded that jeumpa flowers essential oil can be used as perfume agent in stick perfume formulation and stable during the storage in room temperature for 30 days.
Article
Full-text available
The aim of the present study was to optimize flavonoid extraction from Chrysanthemum morifolium and to study the antitumor effects of flavonoids on human gastric cancer MKN45 cells in vitro. A single factor experiment was designed and the extraction process was optimized using an orthogonal test. MKN45 cells were treated with different concentrations of flavonoid from Chrysanthemum morifolium for 24 and 48 h and the inhibitory effect on the MKN45 cells was evaluated using an MTT assay. Following staining with Annexin V-fluorescein isothiocyanate/propidium iodide, flow cytometry was performed. The optimized flavonoid extraction conditions were as follows: Duration of ultrasonic treatment: 35 min; ethanol concentration: 75%; extraction temperature: 80°Cand liquid-to-solid ratio 25: 1. Under the above conditions, the extraction rate of flavonoids was 5.24%. When compared with a blank control group, flavonoids extracted from Chrysanthemum morifolium inhibited the proliferation of MKN45 cells in a dose- and time-dependent manner. Furthermore, in cell groups treated with low, moderate and high concentrations of flavonoid, it was observed that the proportion of apoptotic cells increased in a dose-dependent manner. The extraction process optimized by the orthogonal test achieved a high yield and satisfactory extraction efficiency. Additionally, the experiment demonstrated that flavonoids from Chrysanthemum morifolium inhibited the growth of MKN45 cells and induced their apoptosis. Thus, flavonoids from Chrysanthemum morifolium exerted antitumor effects on MKN45 cells, which may be exploited as a potential antitumor therapeutic for gastric cancer.
Article
Full-text available
Cocoa butter (CB) is the byproduct of cocoa bean processing industry and is obtained from the mature bean from the Theobroma cacao plant. It is an important ingredient in the chocolate and other confectionery industries. It's valued for its unique physicochemical properties which is given by its peculiar fatty acid composition. The major triacylglycerols (TAG) present in CB is symmetrical and contains very less amount of highly unsaturated fatty acid. The major fatty acids present in it are palmitic acid, stearic acid, oleic acid and linoleic acid, but low amounts of lauric acid and myristic acid. Increasing demand and shortage of supply for CB, poor quality of individual harvests, economic advantages and some technological benefits have induce for the development of its alternative called cocoa butter replacer (CBR). In the CBRs the TAG compositions are similar but are not identical to genuine CB. Most of them are produced by either modification of natural fat or by their blending in different proportion. However, it couldn’t satisfy the consumer and fulfill the demand of confectionery industries. This review gives a brief idea about the processing of cocoa pod, the production of cocoa butter and its composition with fats that are commonly used as its Replacers
Preprint
Chrysanthemum morifolium is one of the four major cut flowers in the world and has important ornamental and economic value. Flower fragrance is an important ornamental character of chrysanthemum, especially for tea and edible chrysanthemum, and the excellent fragrance determines its commercial value. At present, however, chrysanthemum is mostly chrysanthemum fragrance and medicinal fragrance, and the research on chrysanthemum flower fragrance mainly focuses on the composition and content of fragrancetic substances, and there is no clear classification of fragrance types. Here, we divided chrysanthemum fragrance into six categories by sensory evaluation, and detected the fragrance components and contents of every representative chrysanthemum with different fragrance types by GC-MS. In addition, we analyzed the genetic conservative substances of fruity fragrance chrysanthemum with multi-functional development potential, which provided a theoretical basis for creating new chrysanthemum germplasm with specific fragrance types, which was conducive to accelerating the biological breeding process of chrysanthemum with new fragrance types.
True experimental study design
  • P Gautam
Gautam, P. (2017, August 29). True experimental study design. Retrieved from https://www.slideshare.net/PrayasGautam2/true-experimental-study-design
Sensitive to alcohol-based perfumes? Here's why you should try using a solid perfume instead
  • G X Ho
Ho., G. X.,. (2022, September 21). Sensitive to alcohol-based perfumes? Here's why you should try using a solid perfume instead. Retrieved from https://www.herworld.com/gallery/beauty/solid-perfumes-sensitive/ Iowa State University. (2023). PH meter -Instrument. Retrieved from https://group.chem.iastate.edu/Holme/augmented-reality-in-educationallaboratories/ph-meter/
Why perfume smells different on different skins
  • C Kessler
Kessler, C.,. (2019, December 12). Why perfume smells different on different skins. Retrieved from https://www.ceenta.com/news-blog/why-does-perfume-smell-different-on-myskin
What is cacao butter? Everything you need to know
  • C Magazine
Magazine, C. (2022, August 30). What is cacao butter? Everything you need to know. Retrieved from https://readcacao.com/what-is-cacao-butter/