Medicinalplantsafetyisarisingchallengeworldwideduetothecontinuedoveruse ofpesticidestotheirmaximumresiduelimits.Duetothehighdemandformedicinal plants, theirproduction isbeing increasedandsometimesprotectedbypesticide use.Theanalysisoftheseresiduesrequiresrobustanalyticalmethodstoensurethe safetyandqualityofmedicinalplants.Developingeffectivesamplepreparationfor detectingpesticidesischallenging,duetotheirdiversenatures,classes,andphysicochemical characteristics.Hence, existingtechniquesandstrategiesareneededto improve the reliabilityof the results. The reviewdiscusses thecurrent stateof samplepreparationtechniques, analyticalmethods, and instrumental technologies employed in pesticide residue analysis in medicinal plants. It highlights the challenges, limitations, andadvancements inthe field, providing insights intothe analytical strategies used to detect and quantify pesticide residues. Reliable, accessible, affordable, andhigh‐resolutionanalytical procedures areessential to ensure that pesticide levels inmedicinal plants are effectively regulated. By understanding thecomplexitiesof pesticide residueanalysis inmedicinal plants, this reviewarticleaims tosupport theconservationofmedicinal plant resources, promotepublichealth,andcontributetothedevelopmentofsustainablestrategies for ensuring thesafetyandqualityofmedicinal plants inNepal. The findingsof thisreviewwillbenefitresearchers,policymakers,andstakeholders involvedinthe conservationofmedicinalplantresourcesandthepromotionofpublichealth. KEYWORDS analytical techniques,medicinalplants,pesticides, samplepreparation, toxicity