The goals of the present work were to document the weed species in Triticum aiestivum L. (Wheat) fields in MR agricultural project Masiklo and to provide quantitative data that could be used to estimate losses due to weeds, also to attract the attention to the economically important weeds in MR agricultural project Masiklo and to develop methods for their control. In this study, a survey was conducted to identify weed species naturally occurring in MR agricultural Wheat fields in Masiklo Libya. A checklist including 41 species was prepared. The checklist includes the scientific name for each species. Reported species were belonging to 37 genera and 19 families of angiosperms. Dicotyledons were represented by 32 species, 30 genera, and 17 families, and Monocotyledons were represented by 9 species, 7 genera, and 2 families. The dominant families according to the number of species were Poaceae (8 species), Fabaceae (6 species), Asteraceae and Brassicaceae (5 species), and Apiaceae (3species). The results of this survey showed that Lolium rigidum, Fumaria officinalis, Hordeum murinum ssp. glaucum, Medicago polymorpha, Bromus madritensis, Bromus rigidus and Sinapis flexuosa were the dominant species in studied fields.