No full-text available
To read the full-text of this research,
you can request a copy directly from the authors.
DETERMINATION OF BIOFILM FORMATION ABILITY OF REPRESENTATIVES OF MICROBIOCENOSE OF BATTLE WOUNDS
Abstract
The article presents experimental data on the study of the ability to form biofilms of clinical strains of bacteria isolated from combat wounds. The search results regarding the determination of MBС dioxidin in relation to clinical strains – representatives of different taxonomic groups are provided. the dynamics of formation of resistance to dioxidin was determined using the reference strains S. aureus ATCC25923, P. aeruginosa ATCC27853. The purpose evaluation of the ability to form biofilms of dominant representatives of the microbiocenosis of combat wounds and the effect of dioxidin on their expression level Materials and methods. In general, the ability to form biofilms of 20 clinical strains of pathogens was determined (S. haemolyticus – 8 samples; K. pneumoniae – 4 samples; E. coli, P. aeruginosa and S epidermidis – 2 samples each and C. amalonaticus and S.aureus – each one) and 3 reference cultures: E. сoli ATCC25922, S. aureus ATCC25923, P. aeruginosa ATCC27853 and the effect on their formation of dioxidin solution with determination of MBK against planktonic cells of these strains Research results: It was established that both reference and clinical strains of microorganisms were characterized by medium and high ability to form biofilms. According to the results of the conducted studies, it was shown that the drug dioxydin showed antimicrobial activity against the majority of planktonic cells of all gram-negative bacteria used in the work, with an MBC from 0.04 to 0.63 mg/ml. The lowest level of activity of the drug was noted against strains of staphylococci (MBC from 1.25 to >2.5 mg/ml), except for planktonic cells of strain S. aureus № . 2093, for which the MBC of dioxidin was 5 mg/ml. In the course of the study, an analysis of the dynamics of the formation of resistance to dioxidin in microorganisms of different taxonomic groups was carried out. S. aureus ATCC25923 strain formed a variant resistant to dioxidin (MBK ≥ 10 mg/ml), while P. aeruginosa ATCC27853 strains did not form such variants, which indicates the possibility of using dioxidin in the long-term therapy of wound infection. Conclusion. The obtained research results demonstrate the microbiological effectiveness of dioxidin, which can be used in the complex treatment of microbial complications of wound injuries
Provide updated data on the antimicrobial activity and chemical composition of original dry extracts from Lavandula angustifolia herb of Ukrainian origin. The aim – an experimental comparative study of the chemical profile and antimicrobial activity of the original dry extracts of Lavandula angustifolia herb and their effect on the ability to destroy biofilms of microbial cultures or prevent their formation in vitro. Materials and methods. The objects of the study are dry extracts obtained from the lavender herb with purified water and ethanol solutions (40 and 70 %). The main biologically active substances (BAS) of the extracts were determined by the Thin-layer chromatography and Absorption spectrophotometry methods. The microbiological properties of the test samples of the investigated plant extracts were studied in vitro by the two-fold serial dilutions method. The ability of microorganisms to form a biofilm was determined by the method of adhesion to polystyrene in flat-bottomed plastic plates. The optical density of the initial bacterial suspension was measured on the Densi-La-Meter device, and the density of inoculated bacterial cells on the Multiskan EX photometer at a wavelength of 540 nm. The study of the antimicrobial activity of water and ethanol extracts of lavender herb in a wide range of concentrations was carried out by the agar diffusion method in the "wells" modification, which is commonly used in microbiological practice. Results. Water and water-ethanol extracts of lavender of Ukrainian origin were obtained. Terpenoids (linalool, linylyl acetate and traces of 1,8-cineol), flavonoids (hyperoside, isoquercitrin), and hydroxycinnamic acids (rosmarinic, chlorogenic acids) were identified in the extracts. The total content of phenolic compounds is 2.02–2.60 mg/g, flavonoids – 1.46–3.17 mg/g. The largest amount of BAS was extracted with 70 % ethanol. According to the results of experimental studies, the extracts of the lavender herb, obtained by extraction with a water-ethanol solution (40 and 70 % ethanol) at a concentration of 1 mg/ml, have antimicrobial properties against a wide range of infectious agents (S. aureus, E. coli, K. pneumoniae, P. aeruginosa, C. albicans). Studies of the influence of test samples of lavender extracts at a concentration of 1 mg/ml on the ability of microorganisms (S. aureus, E. coli, K. pneumoniae, P. aeruginosa) to form biofilms demonstrated that the highest inhibitory activity against biofilm formation was found in the case of the action of test of a sample of phytoextract obtained by extraction with a water-ethanol solution (40 % ethanol), which accounted for S. aureus ‒ 57.8 %, P. aeruginosa – 66.7 %. A wide spectrum of antimicrobial action was established for the tested lavender phytoextracts under the conditions of application of the concentration range of 10-60 μg/ml. The best spectrum of antimicrobial action and the highest activity corresponds to the lavender extract, obtained by extraction with 70 % ethanol, with the effect depending on the concentration. Conclusion. The lavender herb of Ukrainian origin is a promising and affordable source of potential antimicrobial active pharmaceutical ingredients (API). Water-ethanol lavender extract (70 % ethanol), according to research results, has shown high antimicrobial and antifungal potential. According to preliminary data, antimicrobial activity correlates with the content of phenolic compounds. The obtained results may be useful for the search for original substances for the complex correction of symptoms of neurological deficits of infectious etiology
Achromobacter species are isolated from rare but severe healthcare-associated infections, including surgical site infections. They are considered to preferentially infect immunocompromised patients but so far with limited evidence. We conducted a systematic review on Achromobacter spp. surgical site infections (SSIs) to determine if such infections were indeed more commonly associated with immunocompromised patients. The secondary objective was to describe the characteristics of infected patients. Eligible articles had to be published before 30 September 2020 and to report Achromobacter spp. SSIs across all surgical specialties excluding ophthalmology. Analyses were performed on individual data without meta-analysis. Cases were divided into 2 subgroups: one group which had either prosthesis or implant and the other group which did not. A first selection led to a review of 94 articles, of which 37 were analyzed. All were case reports or case series and corresponded to 49 infected patients. Most of the patients were under 65 years of age and had undergone a heart or digestive surgery followed by deep infection with no co-infecting pathogens. Nine out of the 49 cases were immunocompromised, with similar distribution between the two subgroups (16.6% and 20%, respectively). This review suggests that Achromobacter spp. SSIs do not preferentially target immunocompromised patients.
The main factor in the treatment of suppurative and inflammatory processes is the timely optimization of treatment measures taking into account the nature of the microflora and its susceptibility to antimicrobial drugs. The purpose of the study is to monitor the spectrum of microorganisms – pathogens of purulent-inflammatory processes of soft tissues in surgical patients; study of the sensitivity of isolated strains to antibiotics. The material was collected in accordance with aseptic rules. The identification of a pure culture of bacteria was carried out according to morphological, culture, biochemical properties, and the presence of virulence enzymes. Sensitivity of bacteria to antibiotics was determined by the standard disks method (by Kirby-Bauer’s). 255 patients with purulent-inflammatory processes of soft tissues were examined for the period from 2014 to 2017. 229 strains of isolated bacteria were included to Escherichia coli, Citrobacer freundii, Enterobacter cloacae, E.aerogenes, Proteus vulgaris, Staphylococcus aureus, S.epidermidis, Streptococcus pyogenes, S.viridians, S.agalactiae, Pseudomonas aeruginosa. The main cause of purulent-inflammatory processes of soft tissues is Staphylococci (67,2%). Compared to previous studies, the number of P.aeruginosa isolated cultures increased (7.9%). In monoculture and in association with other microorganisms, E. coli (9.6% of cases), E.cloacae et aerogenes (3.9% of cases), P.vulgaris (3.9% of cases), C.freundi (2.5% of cases), S.agalactiae, S.pyogenes, S.viridans (3.5%). The number of associated sows reaches 12%. Clinical strains of microorganisms remain most sensitive to fluoroquinolones, cephalosporins, and also retains high sensitivity to gentamicin, lincomycin, rifampicin, which is important for empirical antibiotic therapy. To increase the effectiveness of antibacterial therapy, strict adherence to the mode of appointment of antibiotics, justification of indications, a combination of antibiotics of different spectrum of action, mandatory correction after determining the sensitivity of the pathogen.
The mechanisms by which bacteria resist killing by antibiotics and biocides are still poorly defined, although repeated exposure
to sublethal concentrations of antibacterial agents undoubtedly contributes to their development. This study aimed both to
investigate the potential of Salmonella enterica and Escherichia coli O157 for adaptive resistance to commonly used biocides and to determine any cross-resistance to antibiotics. Strains were
repeatedly passaged in media containing increasing concentrations of a biocide or antibiotic until adaptive resistance was
obtained. A wide panel of antimicrobial agents was then screened by using the adapted strain to determine cross-resistance,
if any. Adaptive resistance was readily achieved for both S. enterica and E. coli O157. Cross-resistance in adaptively resistant S. enterica varied with the serotype; Salmonella enterica serovar Enteritidis expressed cross-resistance to chloramphenicol, whereas Salmonella enterica serovar Typhimurium expressed cross-resistance to chlorhexidine. Benzalkonium chloride-resistant Salmonella enterica serovar Virchow showed elevated resistance to chlorhexidine; however, chlorhexidine-resistant Salmonella serovar Virchow did not demonstrate reciprocal cross-resistance to benzalkonium chloride, suggesting specific rather than
generic resistance mechanisms. E. coli O157 strains acquired high levels of resistance to triclosan after only two sublethal exposures and, when adapted, repeatedly
demonstrated decreased susceptibilities to various antimicrobial agents, including chloramphenicol, erythromycin, imipenem,
tetracycline, and trimethoprim, as well as to a number of biocides. These observations raise concern over the indiscriminate
and often inappropriate use of biocides, especially triclosan, in situations where they are unnecessary, whereby they may
contribute to the development of microbial resistance mechanisms.
Таксономічний склад, популяційний рівень і мікроекологічні показники мікробіоти ранового вмісту вогнепальних поранень та мінно-вибухових травм
- В В Бендас
- Я П Стефак
- В Мойсюк
Бендас В. В., Стефак Я. П., Мойсюк В. Д. Таксономічний склад, популяційний рівень і мікроекологічні показники мікробіоти ранового вмісту вогнепальних поранень та мінно-вибухових травм. Клін. експеримент. патол. 2019. № 18(2). С. 13-18.
Universum: медицина и фармакология: электронный научный журнал
Изучение антимикробной активности мазей, содержащих диоксидин, на стандартных
штаммах основных возбудителей раневой инфекции [Электронный ресурс] / Е. А. Штанюк,
В. В. Минухин, Н. А. Ляпунов и др. Universum: медицина и фармакология: электронный научный журнал. 2014. № 5 (6). Раздел 43: Химиотерапия и антибиотики
Мікробіологічна характеристика інфекційних ускладнень бойових поранень в різних збройних конфліктах. Укр. журн. мед. біол. спорт
- В М Кондратюк
Кондратюк В. М. Мікробіологічна характеристика інфекційних ускладнень бойових поранень в різних збройних конфліктах. Укр. журн. мед. біол. спорт., 2017. № 3 (7). С. 219-223.
Статистические методы в медико-биологических исследованиях с использованием Excel
- С М Лапач
- А В Чубенко
- П Н Бабич
Лапач С. М., Чубенко А. В., Бабич П. Н. Статистические методы в медико-биологических исследованиях с использованием Excel. К.: МОРІОН. 2001. 408 с
Summary of a systematic review on screening for extended spectrum beta-lactamase and the impact on surgical antibiotic prophylaxis. Global Guidelines for the Prevention of Surgical Site Infection
Web Appendix 4. Summary of a systematic review on screening for extended spectrum
beta-lactamase and the impact on surgical antibiotic prophylaxis. Global Guidelines for the
Prevention of Surgical Site Infection. World Health Organization, 2018.
References
Taksonomichnuy sklad, populyatchiynuy riven i microekologichni pokaznyky mikrobioty ranovogo vmisty vognapalnykh poranen ta minno-vybukhovykh travm [Taxonomic composition, population level and microecological indicators of the microbiota of wound contents of gunshot wounds and mine-explosive injurie
- V V Bendas
- Stefak Yap
- V D Moysuk
Bendas VV, Stefak YaP, Moysuk VD. Taksonomichnuy sklad, populyatchiynuy riven i microekologichni pokaznyky mikrobioty ranovogo vmisty vognapalnykh poranen ta minno-vybukhovykh travm [Taxonomic composition, population level and microecological indicators of the microbiota of wound contents of gunshot wounds and mine-explosive injurie]. Clin. Exsperyment.
patol. 2019, 18(2): 13-18.
Biologichni vlastyvosti osnovnykh zbudnykiv gniyno-zapalnykh zakhvoruvan u khirurgichnykh khvorykh Zaporizkoi likarni shvydkoi dopomogy [Biological properties of the main causative agents of purulent-inflammatory diseases in surgical patients of Zaporizhzhya Emergency Hospital
- N M Polichshuk
- D L Kyryk
- I E Urchuk
Polichshuk NM, Kyryk DL, Urchuk IE et al. Biologichni vlastyvosti osnovnykh zbudnykiv
gniyno-zapalnykh zakhvoruvan u khirurgichnykh khvorykh Zaporizkoi likarni shvydkoi dopomogy
[Biological properties of the main causative agents of purulent-inflammatory diseases in surgical patients of Zaporizhzhya Emergency Hospital]. Aktualni putannya farmatchevtychnoi nauku
tapraktyky. 2020; 2(33): 271-277.
Vplyv antybiotykiv na bioplivkoutvorennya MSSA nf MRSA shtamamy stafilokoka [Effect of antibiotics on biofilm formation by MSSA and MRSA strains of staphylococcus
- S A Derkach
- I A Voronkina
- Ls Et Habysheva
Derkach SA., Voronkina I. A., Habysheva LS. et.al Vplyv antybiotykiv na bioplivkoutvorennya
MSSA nf MRSA shtamamy stafilokoka [Effect of antibiotics on biofilm formation by MSSA and
MRSA strains of staphylococcus].Infektchiyni tchvoroby. 2017. (4): 46-51.
Microbiologichna kharakterystyka infektchiynykh usklzdnen boyovykh poranen v riznych zbroynukh konfliktakh
- V M Kondratuk
Kondratuk VM. Microbiologichna kharakterystyka infektchiynykh usklzdnen boyovykh poranen v riznych zbroynukh konfliktakh [Microbiological characteristics of infectious complications of combat wounds in various armed conflicts]. Ukr.zhurn.med.sport. 2017; 3(7): 219-223.
Statisticheskie metody v mediko-biologicheskich issledovaniyach s ispolzovaniem Excel [Statistical methods in medical and biological research using Excel
- S M Lapach
- A V Chubenko
- P N Babich
Lapach SM, Chubenko AV, Babich PN. Statisticheskie metody v mediko-biologicheskich
issledovaniyach s ispolzovaniem Excel [Statistical methods in medical and biological research
using Excel]. K: Morion. 2001: 408 s.
Microbiologichni aspekty ranovoi infektchii u poterpilykh vnaslidok boyovyrh diy ta ii kompleksne likuvannya z zastosuvannyam vacuum-terapii [Microbiological aspects of wound infection in combat victims and its complex treatment using vacuum therapy
- M D Zheliba
- A V Verba
- G L Bogusch
Zheliba MD, Verba AV, Bogusch GL et al. Microbiologichni aspekty ranovoi infektchii u poterpilykh vnaslidok boyovyrh diy ta ii kompleksne likuvannya z zastosuvannyam vacuum-terapii
[Microbiological aspects of wound infection in combat victims and its complex treatment using
vacuum therapy]. Suchasni medichni tekhnologii. 2019; 3: 50-55.
Mikroflora suchasnoi boyovoi rany taii chutluvist do antybiotykiv. Chastyna I [Microflora of a modern combat wound and its sensitivity to antibiotics. Part I
- O O Fomin
- N S Fomina
- V P Kovalchuk
- S A Aslanyan
Fomin OO, Fomina NS, Kovalchuk VP & Aslanyan SA. Mikroflora suchasnoi boyovoi rany
taii chutluvist do antybiotykiv. Chastyna I [Microflora of a modern combat wound and its sensitivity to antibiotics. Part I]. Ukr.Med.Chasopus. 2023; 3(155): 82-85.
Rol enterokokiv u vynyknenni nozokomialnykh infektsii
- E G Peretyatko
Peretyatko EG. Rol enterokokiv u vynyknenni nozokomialnykh infektsii (ohliad literatury)
[Role of enterococci in the occurrence of nosocomial infections]. Annals of Mechnikov Institute.
2013;4: 22-27.
et al Strymuvannya antymikrobnoi rezystentnosti na pidkhodach «edyne zdorovya: monographiya [Containment of antimicrobial resistance using «One Health» approaches: Monograph
- A G Salmanov
- D V Shcheglov
- V V Artemenko
Salmanov AG, Shcheglov DV, Artemenko VV. et al Strymuvannya antymikrobnoi rezystentnosti na pidkhodach «edyne zdorovya: monographiya [Containment of antimicrobial resistance
using «One Health» approaches: Monograph]. K: AhrarMediaGrup. 2022: 380 s.
World Health Organization. Web Appendix 4. Summary of a systematic review on screening for extended spectrum beta-lactamase and the impact on surgical antibiotic prophylaxis
- L B Rodrigues
- Dos Santos
- L R Tagliari
Rodrigues LB., Dos Santos LR., Tagliari VZ et.al Quantification of biofilm production on
polystyrene by Listeria, Escherichia coli and Staphylococcus aureus isolated from a poultry slaughterhouse. Braz. J. Microbiol. 2010; 41(4): 1082-5.
15. World Health Organization. Web Appendix 4. Summary of a systematic review on screening for extended spectrum beta-lactamase and the impact on surgical antibiotic prophylaxis. In
Global Guidelines for the Prevention of Surgical Site Infection. 2018.