The genus Dieffenbachia Schott has approximately 135 species, most of them occurring in South America. Major centers of diversity for the genus include Colombia with 37 species, Ecuador (34), Peru (30), Brazil (27), Panama (20), and Costa Rica (13). There are 26 species in Central America, with 20 species (77%) new to science. These are D. burgeri Croat & Grayum, D. copensis Croat, D. crebripistillata Croat, D. davidsei Croat & Grayum, D. fortunensis Croat, D. fosteri Croat, D. galdamesiae Croat, D. horichii Croat & Grayum, D. isthmia Croat, D. killipii Croat, D. lutheri Croat, D. nitidipetiolata Croat, D. obscurinervia Croat, D. panamensis Croat, and D. standleyi Croat described herein and D. beachiana Croat & Grayum, D. concinna Croat & Grayum, D. grayumii Croat, D. hammelii Croat & Grayum and D. tonduzii Croat & Grayum described elsewhere. Most species range from Nicaragua to Panama. Belize has only 1 species of Dieffenbachia; Mexico, El Salvador, and Guatemala have 2 species, followed by Honduras (3), Nicaragua (6), Costa Rica (13) and Panama (20). Only a few Central American species could be considered widespread. Among the most widespread are D. oerstedii Schott and D. wendlandii Schott, both of which range from Mexico to Panama, as well as D. nitidipetiolata and D. tonduzii, which range from Honduras to Ecuador. Species endemism is high, especially in Costa Rica (3) and Panama (9). A total of 9 species are shared between Panama and Costa Rica. Eight species, almost 31% of the total, range into South America. These are D. davidsei, D. isthmia, D. killipii, D. longispatha, D. nitidipetiolata D. obscurinervia, D. seguine, and D. tonduzii. Most of these only extend to Colombia, but three species D. killipii, D. nitidipetiolata, and D. tonduzii, range to Ecuador. Only D. killipii ranges to the eastern slope of the Andes. Dieffenbachia seguine ranges into Brazil and Bolivia, from the West Indies.