A probability-based procedure is presented to define damage thresholds of bridges’ deterioration curves, which is applied to a large bridge database of real on-site inspections. The procedure is based on a change of variable to transform any distribution of bridge durability deterioration ratings into a normal distribution. This allows probability-based calculations of damage thresholds associated to certain criteria (e.g. Serviceability or Ultimate Limit State), for immediate application to deterioration prediction curves. This research defines a novel procedure for a probability-based analysis of inspection-based data and, de facto, provides a reliable statistical support system to traditional infrastructure management systems of bridges.