The FTIR analysis of Fomes fomentarius ethyl acetate extract-antibiotic interactions in some multi- drug resistant (MDR) E. coli strains having active efflux pumps ORAL PRESENTATION

Abstract

Studies on eliminating multi-antibiotic resistance are continuing and in these studies, the search for inhibitors takes a major role. Due to the toxic nature of synthetic efflux pump inhibitors, new inhibitors having low toxicity are under investigation. Although studies at the in vivo level are long-term, studies at the in vitro level are provided more easily. In this study, inhibitor trials of some multidrug-resistant Escherichia coli strains with active efflux pumps were carried out using ethyl acetate extract of Fomes fomentarius, a macrofungi. In addition, antibiotics were selected according to the antibiotic resistance profile of the strains used, and combined use with the extract was tried. According to the results of the combined use of antibiotics and extracts, FTIR analysis was performed for microorganisms presenting an inhibitory effect. Thus, preliminary studies will be carried out by evaluating the mechanism of action. According to the FTIR results; significant changes were observed between the band <900 cm-1 and 3000 cm-1. While some of these changes occurred in proteins, some changes occurred in DNA/RNA and phospholipids. The obtained data will be detailed and used for further studies.

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5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022) November 23-25, 2022.
www.EurasianBioChem.org
192
ORAL PRESENTATION
The FTIR analysis of Fomes fomentarius ethyl acetate extract-antibiotic interactions in some multi-
drug resistant (MDR) E. coli strains having active efflux pumps
Eda Altınöz1* (ORCID https://orcid.org/0000-0003-1277-2019), Ilgaz Akata2 (ORCID https://orcid.org/0000-0002-
1731-1302), Ergin Murat Altuner1 (ORCID: https://orcid.org/0000-0001-5351-8071)
*1 Kastamonu University, Faculty of Science, Department of Biology, Kastamonu, Turkey.
2 Ankara University, Faculty of Science, Department of Biology, Ankara, Turkey.
*Corresponding author e-mail: altinozedaa@gmail.com
Abstract
Studies on eliminating multi-antibiotic resistance are continuing and in these studies, the search for inhibitors takes a
major role. Due to the toxic nature of synthetic efflux pump inhibitors, new inhibitors having low toxicity are under
investigation. Although studies at the in vivo level are long-term, studies at the in vitro level are provided more easily. In
this study, inhibitor trials of some multidrug-resistant Escherichia coli strains with active efflux pumps were carried out
using ethyl acetate extract of Fomes fomentarius, a macrofungi. In addition, antibiotics were selected according to the
antibiotic resistance profile of the strains used, and combined use with the extract was tried. According to the results of
the combined use of antibiotics and extracts, FTIR analysis was performed for microorganisms presenting an inhibitory
effect. Thus, preliminary studies will be carried out by evaluating the mechanism of action. According to the FTIR results;
significant changes were observed between the band <900 cm-1 and 3000 cm-1. While some of these changes occurred in
proteins, some changes occurred in DNA/RNA and phospholipids. The obtained data will be detailed and used for further
studies.
Keywords: Efflux Pumps, Multidrug Resistance, Bacterial Resistance, Ethyl Acetate, E. coli, FTIR.
Acknowledgment: The authors would like to thank the Kastamonu University Scientific Research Projects Coordination
Department under the Grant No. KÜ-BAP01/2021-47 for the support.