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5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022) November 23-25, 2022.
www.EurasianBioChem.org
191
ORAL PRESENTATION
The Fomes fomentarius methanol extract and antibiotic interactions in some clinically isolated multi-
drug resistant E. coli strains with active efflux pumps by using FTIR analysis
Eda Altınöz1* (ORCIDhttps://orcid.org/0000-0003-1277-2019), Ilgaz Akata2 (ORCID https://orcid.org/0000-0002-
1731-1302), Ergin Murat Altuner1 (ORCID: https://orcid.org/0000-0001-5351-8071)
*1 Kastamonu University, Faculty of Science, Department of Biology, Kastamonu, Turkey.
2 Ankara University, Faculty of Science, Department of Biology, Ankara, Turkey.
*Corresponding author e-mail: altinozedaa@gmail.com
Abstract
Studies to prevent antibiotic resistance are continuing rapidly. As it is known, bacteria have various resistance
mechanisms. The over-expression of efflux pumps is just one of these resistance mechanisms. In antibiotic intake, the
drug taken through this resistance mechanism is expelled out of the cell and the effect of the drug is lost. Various studies
are carried out to prevent this. Inhibition of the efflux pumps is one of these methods. With the inhibitory effect of
compounds, it is aimed to recover the effect of drugs in the in vitro studies. In order to avoid the toxicity problem, the
researches for inhibitors from natural sources are under investigation. In this study, methanol extract of Fomes
fomentarius macrofungus was used to observe its efflux pump inhibition potential in some clinically isolated multidrug-
resistant Escherichia coli strains. Antibiotics were selected according to the antibiotic resistance profile of the strains used
and the study was carried out using a combination with the extract. FTIR analysis was performed to evaluate the
mechanism of action for strains with an inhibitory effect as a result of combined use. According to the FTIR analysis;
there are significant changes in the band scale. The observed changes were found in proteins, DNA/RNA, phospholipids,
polysaccharides, fatty acids, and fingerprint regions. In light of the observed data, it is appropriate to elaborate further
studies.
Keywords: Bacterial Resistance, E. coli, Efflux Pumps, Methanol, FTIR, Multidrug Resistance.
Acknowledgment: The authors would like to thank the Kastamonu University Scientific Research Projects Coordination
Department under the Grant No. KÜ-BAP01/2021-47 for the support.