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Cordyceps sinensis is an edible mushroom found in high quantities in the regions of the Himalayas and widely considered in traditional systems of medicine. It is a non-toxic remedy mushroom and has a high measure of clinical medical benefits including cancer restraint, high blood pressure, diabetes, asthma, depression, fatigue, immune disorder, and many infections of the upper respiratory tract. The cultivation of this kind of mushroom is limited to the region of the Sikkim and to cultivate in the other regions of the country, they are need of investigation and prediction of cordyceps sinensis mushroom lifecycle. From the studies, it is concluded that the precision-based agriculture techniques are limitedly explored for the prediction and growth of Cordyceps sinensis mushrooms. In this study, an internet of things (IoT) inspired framework is proposed to predict the lifecycle of Cordyceps sinensis mushrooms and also provide alternate substrate to cultivate Cordyceps sinensis mushrooms in other parts of the country. As a part of lifecycle prediction, a framework is proposed in this study. According to the findings, an IoT sensor-based system with the ideal moisture level of the mushroom rack is required for the growth of Cordyceps sinensis mushrooms. This is an open access article under the CC BY-SA license.
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International Journal of Electrical and Computer Engineering (IJECE)
Vol. 13, No. 1, February 2023, pp. 1142~1151
ISSN: 2088-8708, DOI: 10.11591/ijece.v13i1.pp1142-1151 1142
Journal homepage: http://ijece.iaescore.com
An internet of things enabled framework to monitor the
lifecycle of Cordyceps sinensis mushrooms
Minakshi Memoria1, Sanjeev Kumar Shah1, Harishchander Anandaram3, Anooja Ali4, Kapil Joshi1
Parag Verma1, Rajesh Singh2,5, Anita Gehlot2,5, Shaik Vaseem Akram2
1Uttaranchal Institute of Technology, Uttaranchal University, Dehradun, India
2Division of Research and Innovation, Uttaranchal Institute of Technology, Uttaranchal University, Dehradun, India
3Centre for Excellence in Computational Engineering and Networking, Amrita Vishwa Vidyapeetham, Coimbatore, India
4Department of Computer Science and Engineering, REVA University, Bangalore, India
5Department of Project Management, Universidad Internacional Iberoamericana, Campeche, México
Article Info
ABSTRACT
Article history:
Received Mar 10, 2022
Revised Sep 4, 2022
Accepted Sep 22, 2022
Cordyceps sinensis is an edible mushroom found in high quantities in the
regions of the Himalayas and widely considered in traditional systems of
medicine. It is a non-toxic remedy mushroom and has a high measure of
clinical medical benefits including cancer restraint, high blood pressure,
diabetes, asthma, depression, fatigue, immune disorder, and many infections
of the upper respiratory tract. The cultivation of this kind of mushroom is
limited to the region of the Sikkim and to cultivate in the other regions of the
country, they are need of investigation and prediction of cordyceps sinensis
mushroom lifecycle. From the studies, it is concluded that the precision-
based agriculture techniques are limitedly explored for the prediction and
growth of Cordyceps sinensis mushrooms. In this study, an internet of things
(IoT) inspired framework is proposed to predict the lifecycle of Cordyceps
sinensis mushrooms and also provide alternate substrate to cultivate
Cordyceps sinensis mushrooms in other parts of the country. As a part of
lifecycle prediction, a framework is proposed in this study. According to the
findings, an IoT sensor-based system with the ideal moisture level of the
mushroom rack is required for the growth of Cordyceps sinensis mushrooms.
Keywords:
Cordyceps sinensis
Environment monitor
Internet of things
Mushroom cultivation
This is an open access article under the CC BY-SA license.
Corresponding Author:
Rajesh Singh
Division of Research and Innovation, Uttaranchal Institute of Technology, Uttaranchal University
Dehradun-248007, Uttarakhand, India
Email: drrajeshsingh004@gmail.com
1. INTRODUCTION
Traditionally, Chinese medical and Tibetan medicine prefer Cordyceps sinensis as a medication for
different diseases [1]. Cordyceps is a generic name derived from the Latin word kordyle meaning club and
ceps meaning head. Cordyceps sinensis is the mixture of a caterpillar and a fungus that can only be found
in Sikkim at altitudes above 4,500 meters [2]. Cordyceps sinensis is typically harvested from April to August
in cold, grassy, alpine meadows of the Himalayan mountains [3]. Traditional North Sikkim healers and
residents use Yarsagumba, Keerajhar (Cordyceps sinensis) alone or in combination with other herbs to
strengthen the immune system, improve renal function in patients with erythematosus, and retard aging [4].
In addition, it is also used to recover from anemia, immune deficiencies, fatigue, low back pain, impotence,
night sweats, and for recuperation from chronic disease. The annual ascomycete Cordyceps sinensis is
closely associated with the mushroom and is not technically a mushroom but is described in traditional
Chinese and Tibetan medicine as an exotic therapeutic fungus [5]. Cordyceps comprise distinct nutritional
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components like amino acids, carbohydrates such as oligosaccharides, monosaccharides, vitamins like K,
B12, B2, and B1.
Even though this plant has numerous medicinal benefits, it is only found in Sikkim. It is difficult for
people in other parts of the country to benefit from it because it grows at high altitudes and in cold climates
[6]. The study of the life cycle of Cordyceps sinensis mushrooms must be carried out in order to understand
the various parameters that are required to cultivate the same mushroom in a different region [7]. As a part of
it, a few studies have proposed frameworks to study the lifecycle of the Cordyceps sinensis mushrooms, but
those frameworks lack a graphical user interface and are not embedded with internet of things (IoT).
Currently IoT gained significant attention in the farming for its real-time sensing, monitoring and provide
valuable insights to the farm to cultivate more effectively [8]. In the mushroom gardening field, IoT is used
for control and observation, and a significant number of readily available structures, particularly for
mushroom development, have yet to be used for general climate screening and control. To overcome these
challenges, we have proposed a framework that is based on IoT, where the lifecycle of caterpillar is studied.
Based upon the study, we can identify those parameters that can be applied in other climate area of India, to
grow this kind of mushroom by studying the lifecycle of caterpillar. The contribution of the study is as a
system is proposed with the integration of big data and IoT framework, that IoT inspired framework is
proposed to study and predict the lifecycle of Cordyceps sinensis and developing dynamic web portal for the
real-time visualization of mushroom processing.
The structure of the paper is organized as follows. Section 2 discusses the literature review.
Section 3 covers the proposed system in detail. Section 4 covers IoT enabled framework and it is concluded
in the final section.
2. PRIOR ART
In these various species of medicinal mushrooms, Cordyceps species are highly considered in
various positive aspects specially in terms of safety or non-toxic group of medicine [9], to enhance the
immune system in terms of human clinical health concern [10], to control neuroprotection based activities
[11], [12] effecting medicine for anticancer [13], activities for antimicrobial system [14], and activates for
anti-inflammatory system as well [15], [16], and therefore, a significant literature review in concern about the
components of medical significances, activities about the pharmacological practices, are hereby covered in
Tables 1 and 2 respectively.
Table 1. Constitution for the medical imperative of Cordyceps sinensis
Ref
Component
Importance
[17]
Cordycepin
Hostility to tumors, inhibition of RNA/DNA association, concealment (anti-HIV) antimalarial
activities of viral replication, regulates homeostatic potential against leukemia action
[18]
Adenosine
Mitigation effect, control of bleeding of cardiovascular arrhythmias
[19]
Amino acids, zinc,
vitamins & trace elements
Struggles with sexual drowsiness
[20]
Polysaccharides
Inhibition of lipid peroxidation, pharmacological agitation, inhibition of hemolysis and tumor
restraint, anti-oxidation action, immunomodulatory, and antitumor property
[21]
Ergosterol
Hostile to the tumor and immunomodulatory effects
[22]
Cordy glucans
Agitation against tumor
Table 2. Important pharmacological practice of Cordyceps sinensis
Pharmacological Activity
Hostility to the effects of asthma and against malignant growth specialist
Activities for possess hypotensive and vasorelaxant
Hostile to oxidation action
Fasting reduces hyperglycemia and immune-regulatory movement
Implementing hostile and invincible modalities for tumor action
This specific species of medicinal mushroom are parasites, essentially on creepy crawlies and other
arthropods. Some species from these are also in parasitic categories on other kind of fungi like additionally
subterranean, truffle-like Elaphomyces and spiders as well [28]. There is an incompatible Sacc., which means
entomophagous parasitic growth of the family of Clavicipitaceae. Caterpillar fungus Cordyceps sinensis (Berk.)
Sacc., is a very well alleged medicinal mushroom species [29]. Like the part, the cutting edge can be seen
jutting slightly above the insect like a tiny horn. This horn-like structure continues to be built even further [30].
The Cordyceps sinensis medicinal mushroom is seasonally produced in India with specific regions
like Sikkim or other high mountain regions, plus it can very well be filled in environmental-controlled crop
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produce houses [31]. As of late, Cordyceps species in brown rice were effectively compromised towards
developing and have been responsible for many examinations refined in brown rice using Cordyceps species
[32], [33]. By and large, the ecological conditions for the growth of Cordyceps sinensis mushrooms can be
expressed in terms of temperature, humidity, light intensity, fresh air and the soil moisture [34]. In the
mushroom growing/cultivation room, the airflow is an important part of mushroom development because it
legitimately affects the carbon dioxide (CO2) content of the room.
The growth pattern of Cordyceps sinensis mushrooms can be divided into two phases; the first phase
is vegetative phase, which includes mycelia expansion and development, and the second phase is the
regenerative phase [35]. To grow Cordyceps sinensis mushrooms, a mycelium culture is allowed to grow on
cleaned cereal grains, which bring forming spawn. The produce spawn is inoculated into a disinfected
substrate and allowed to uproot the substrate [36]. During brooding, mycelium develops all through the
substrate and uses supplements in it. This cycle is called the spawn or production run. During the production
run, the ideal temperature is about 25 °C and the high carbon dioxide concentrate is positive. From that point
on, the mycelium arrives at the regenerative stage and is fitted to deliver mushrooms. The main variables that
induce the formation of Cordyceps sinensis mushrooms are an unexpected decrease in temperature
(temperature of 5 to 10 °C) and a sudden decrease in carbon dioxide fixation [37]. After that, the ideal
temperature for growth is around 10 to 25 °C, 85% to 92% RH (relative humidity), 600 ppm (million per
million), and carbon dioxide fixation under 500 lux, 2,000 lux for 12 hours. Since dissimilar phases required
a specific senescence, temperature, carbon dioxide, and the concentration of carbon dioxide, a framework
with pre-determined conditions for different phases simplifies it to a Cordyceps sinensis mushroom farmers
[38]. All these referenced functions do not allow checking and control through the graphical user interface.
Similarly, IoT can be easily used for control and observation in the mushroom gardening field. Similarly, a
significant number of accessible structures in the market, especially for mushroom development, are not yet
employed for general climate screening and control.
3. PROPOSED SYSTEM
The advancement in technology in the area of farming has transformed the monitoring of farming in
real-time environment from any location through internet connectivity. As limited real-time technology was
used, this encouraged the implementation of IoT to predict the growth of Cordyceps sinensis mushrooms in
this study. The proposed framework is based on the growth of Cordyceps sinensis mushrooms using the tail
of a caterpillar, and it will pave the way for the recommended substrate for Cordyceps sinensis mushrooms.
The proposed framework is illustrated in Figure 1, and it comprises of five components such as substrate
model, big data warehousing, cloud IoT module, IoT enabled framework, and mobile app. Despite the fact
that much work has been done on alternate substrate for yield of Cordyceps sinensis Mushrooms, there is
limited data on the alternate substrate for growth of Cordyceps sinensis mushrooms. An alternative substrate
for the cultivation of Cordyceps sinensis Mushrooms other than the lab variants is suggested with the
assistance of proposed framework.
Big Data
Warehousing IoT enabled
framework
Govt.
Internet
Mobile
App
Substrate
Module
- Farmers
- Vendors
- Agro Marketing Agencies
Figure 1. Proposed framework on between big data and IoT framework
IoT enabled framework is portable IoT device with soil and environment sensors. Mobile app
module provides interface to the users, and it will also notify the users or farmers through text messages.
Storage, big-data mining, analysis, and knowledge building engines, as well as an application module to
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interface with users, make up the cloud IoT module. Big data warehousing is generally used to store and
mine the data which is relevant to the users and knowledge building module is used to take the decision
based on the prediction. Since the inherent benefits of cultivation of Cordyceps sinensis mushrooms are high,
the suggested module saves data collected on a regular basis from soil and environmental samples. The cloud
IoT model is sandwiched between the big data warehousing engine. This module is critical in making
decisions based on current weather conditions, crop yield predictions, best crop sequence analysis based on
data accumulated over time, best crop for corresponding soil attributes, and watering requirements based on
soil moisture level. This database makes recommendations to farmers for crops to plant on acreage with
unusual soil features based on prior mushroom stock and current market demand. Big data analysis can be
used to predict future output of each product based on existing knowledge.
4. IoT ENABLED FRAMEWORK
The IoT-enabled framework is a critical component in the proposed system as it is in charge of soil
sampling at regular intervals to obtain soil property data. Figure 2 illustrates the proposed IoT framework in
which it comprises of multiple sensing nodes to sense and monitor the environmental parameters of structure
rack of mushroom. Temperature, humidity, and CO2 convergence are the environmental parameters that are
required to be continuously monitored through the sensing node. Based on these parameters, the water supply
through solenoid valve is controlled by sensing node through relay. The sensing node is connected to the
structure rack through wireless communication protocol.
Sensing node
‘1
Sensing node
‘3’
Structure
racks
Solenoid valve ‘1’
Sensing node
‘2
Sensing node
‘4’
Solenoid valve ‘2’
Solenoid valve ‘3’
Solenoid valve ‘4’
Control
the
valve
Control the valve
Sensor
data
Sensor
data
Wireless
communication
Relay
Relay
Wireless
communication
Wi-Fi based
node
Cloud
Server
Dynamic
web portal
Figure 2. IoT enabled framework
The conveyer line framework is used to reduce human differences with the goal that it can
computerize to measure mushroom growth. The user interface connected to the fundamental regulator is the
dynamic site intended to automate the cycle. The system of conveyer line will be used for laying fertilizer;
these are the fraction or racks on which mushrooms have been developed. These fertilizers are coming from
purification rooms and should be kept in rooms where mushrooms should grow. On the off chance that the
wet matter of the bed is replaced by the ideal requirement, an intricate water system framework is used
flexibly for watering at that point. The information of sensing node is updated to the cloud server through
Wi-Fi based node that is based on internet connectivity. Information running in the cloud will be refreshed at
regular intervals. As indicated by continuous information, temperature activation, humidity, CO2 focus, and
bed moisture content (if necessary) are carried out, which suggests that it reports to the regulator about the
continuous information and orders. A dynamic web portal is designed using Django as our production tool to
automate the entire cycle. Additionally, a conveyer belt framework is planned to minimize human arbitrage
for this entire cycle.
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4.1. Sensing node with sub-controller
The sensing node is critical because it is the primary reason for the IoT-based framework to collect
soil samples at regular intervals in order to obtain soil property data. It is an IoT enabled device with memory
and computing capabilities, as well as a GPS sensor to detect positional data. The soil nutrient sensor devices
that are attached to it are the main components of this kit. The soil pH sensor, soil moisture sensor,
phosphorus (P), potassium (K), and nitrate (N) sensors are interfaced to the sub-controller. The sensing node
performs two different tasks such as monitoring and control. In the monitoring, the sensing of the information
begins with the distinct sensors like temperature and humidity sensor, CO2 sensor, light-dependent resistor
(light dependent resistors (LDR) sensor), and soil moisture sensor. This sensing node detects clock-like
information and updates the same as par with the cloud and sends similar information to the cloud regularly
and updates the information running at regular intervals as the signal cycle completes and automation is done
in similar manner. The electrical characteristics of the MG-811 gas sensor suggest that the more
predominantly predicted CO2 focus will create a more modest output voltage. Table 3 illustrates the
Environmental conditions in different operating modes
Table 3. Environmental conditions in different operating modes
Parameter
Mode of Spawn-run
Initialization mode of Pin head
Mode of Cropping
Temperature
25 °C
19 °C
20 to 25 °C
Humidity
90.0% RH
95.0% RH
85.0% RH
Carbon dioxide
20,000 𝑝𝑝𝑚
600 𝑝𝑝𝑚
<600 𝑝𝑝𝑚
Soil Moisture
48%
52%
68%
Light intensity
Off
2,000 lux per 12 Hrs.
Above 500 lux per 12 Hrs.
4.2. Main controller
The fundamental controller is our Raspberry Pi 3B+ regulator module, with ESP8266 fitted to prod
all sensing nodes. The continuous information state of each dark room where mushrooms grow is brought to
the main controller, for example, the central processing unit or brain of morphology by the cloud. For
solenoid valves in each room, associations are made for dripline irrigation systems.
Dripline irrigation system structures are used on the basis that mushrooms require exceptionally low
amounts of water. The solenoid works so that the control relay to turn on the valve comes from the module.
The solenoid opens and water is given flexibly, similarly, it should be noted that bed moist matter is detected
at regular intervals and is reported to the regulator. Assuming the water required for the mushroom racks is
measurable, it should be noted that the solenoid is modified in this exploration to kill the solenoid when the
bedwetting material approximates Y-2 must be given so that no one is inauspicious. Mushroom beds are
being provided with a measurement of water that can damage yields.
4.3. Dynamic web portal
Figure 3 represents the dynamic web portal work employed in the research work. The dynamic web
portal intended for the framework will go as the user interface for the framework. Likewise, it will be the
official site for a mushroom processing plant or association that will have various interfaces, for example,
client interface, employee interface, and administrator interface.
This dynamic website page is planned to be used in the front end of web planning progress, for
example, HTML, CSS, and JS. In the backend, the database is planned to use My SQL innovation. The front
end and back end have been combined with the use of the Python web structure Django. The front-end
framework for user interface is designed through the use of HTML, CSS, and Java Script. All progress in the
framework will be finished by the interface on this page. A login option on the site page which that has three
major system whereas customer login, employee login, and administrator login user interface. Customer
login is for buyers to request this medicinal mushroom, if they need to enroll first in another customers
signature, they can play the activities they need. On the off chance that an old customer signs up, they can see
the status of their request, the history of the request, and so forth, similar to an e-business site. At this point
when a worker signs up, he can see the subtleties of his work, asking a representative of a business board to
enter a new request, income status, a number of new customers joined, account status, and after this, a similar
location when a person signs in from a particular office, they can see the status of ecological boundaries, for
example, temperature, viscosity, carbon dioxide determination, of every dim room. The moist material of the
bed develops independently and now feels that the moisture content of the bed is low, and it switches water
flexibly on the information.
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Figure 3. Dynamic web portal of the system
5. HARDWARE DESCRIPTION
In this section, we discuss the hardware description of the sensing node. As discussed above, the
sensing node comprises of a controller unit, distinct sensors, wireless communication protocol, relay, and
battery power supply. In the sensing node as shown in Figure 4, ATmega 328P controller is selected for
processing the sensory data and based on sensory data, the components like solenoid valve are controlled
through relay. DHT11 sensor interfaced to the controller act as input, and it sense the temperature and
humidity of the structure racks of the mushroom. Soil moisture sensor and BH1750 light intensity sensor are
also interfaced to the controller to provide the soil moisture content and light intensity as input data. The
sensor data is communicated through the wireless communication protocol to Wi-Fi based node. The wireless
communication protocol is selected on the basis of the transmission range. In case if the data needs to be
transmitted to the short range, then the Zigbee and Wi-Fi module can be embedded in sensing node for data
transmission. In case if the data needs to be transmitted to the long-range, then LoRa communication protocol
can be embedded in sensing node for data transmission. In the power supply, there will be different voltage
converters to match the operating voltage of the components integrated in the sensing node for proper
functioning.
Figure 5 illustrates the Wi-Fi based node enables the sensing node to transmit the data on the cloud
server through internet connectivity. Zigbee/LoRa connected to the sensing node act as transmitter and in the
same way, the Zigbee/LoRa will be embedded in Wi-Fi based node as receiver to receive the data. ESP 8266
Wi-Fi module embedded in this node enables the transmitting data on the cloud server through internet
connectivity. External power supply supplied to Wi-Fi based node is +12 V, but the operating voltage for the
Zigbee/LoRa and ESP8266 Wi-Fi module is +3.3 V, so there is requirement of two different +3.3 V voltage
converters for proper functioning of the communication module.
ATMega 328P
Controller Unit
DHT 11 sensor
(Temperature
+ humidity)
Soil moisture
sensor
BH 1750 Light
intensity
sensor
Input
Sensor
Solenoid Valve
Relay
Output
Battery power
supply
+5V Power
supply
Zigbee/LoRa/
Wi-Fi
+3.3 V /5V
Power supply
FTDI
cable
Figure 4. Hardware description of sensing node
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ATMega 328P
Controller Unit
Zigbee/
LoRa
Battery
power supply
Wi-Fi
module
FTDI
cable
Figure 5. Hardware description of Wi-Fi based node
The sensing node and Wi-Fi based node of the proposed system comprises the ATmega 328P
controller. The ATmega 328P controller will be programmed through Arduino IDE that is based on the C++
language. FTDI port interfaced to the ATmega 328P controller in sensing node and Wi-Fi based node
enabled to program with necessary instructions based on application. Moreover, the node mapping feature
can be embedded in the sensing node to transmit the data if the previous value is more or less than present
value. Symmetric encryption enables encrypting the data and same encryption in Wi-Fi based node enables to
decrypt the data.
6. CONCLUSION
Mushrooms from the Cordyceps sinensis have been proven to improve immune function, reduce the
effects of ageing, promote longer life, and improve liver function in people. The cultivation and growth of
this kind of mushrooms are limited to Sikkim in India and it is difficult to many people to gets its medicinal
benefits. The investigation and prediction of Cordyceps sinensis life cycle is limitedly carried out in previous
studies with IoT enabled framework. To overcome these challenges, this study proposed to implement an IoT
framework to predict life cycle and provide alternate substrate to cultivate Cordyceps sinensis mushrooms in
other parts of the country. As part of predicting lifecycle, the framework is proposed. From the study it is
conclude with IoT sensor-based system with ideal moisture level in mushroom rack is required to grow this
kind of mushroom.
REFERENCES
[1] G. Daba, The endless nutritional and pharmaceutical benefits of the Himalayan gold, cordyceps; current knowledge and
prospective potentials, Asian Journal of Natural Product Biochemistry, vol. 18, no. 2, 2020.
[2] S. Panicker, Cordyceps the fungal gold - a review, Advances in Research, vol. 11, no. 3, pp. 116, Jan. 2017, doi:
10.9734/AIR/2017/35923.
[3] Q. Yuan et al., Extraction, structure and pharmacological effects of the polysaccharides from cordyceps sinensis: a review,
Journal of Functional Foods, vol. 89, Feb. 2022, doi: 10.1016/j.jff.2021.104909.
[4] X. Li et al., A breakthrough in the artificial cultivation of Chinese cordyceps on a large-scale and its impact on science, the
economy, and industry, Critical Reviews in Biotechnology, vol. 39, no. 2. Taylor & Francis, pp. 181191, 2019, doi:
10.1080/07388551.2018.1531820.
[5] G. Das et al., Cordyceps spp.: a review on its immune-stimulatory and other biological potentials, Frontiers in Pharmacology,
vol. 11, Feb. 2021, doi: 10.3389/fphar.2020.602364.
[6] A. Banerjee, P. Chakraborty, and R. Bandopadhyay, Urgent conservation needs in the Sikkim Himalaya biodiversity hotspot,
Biodiversity, vol. 20, no. 23, pp. 8897, 2019.
[7] A. T. Anyu, W.-H. Zhang, and Q.-H. Xu, Cultivated cordyceps: a tale of two treasured mushrooms, Chinese Medicine and
Culture, vol. 4, no. 4, Art. no. 221, 2021.
[8] S. Bhetwal, S. Chatterjee, R. R. Samrat, M. Rana, and S. Srivastava, Cordyceps sinensis: Peculiar caterpillar mushroom, salutary
in its medicinal and restorative capabilities, The Pharma Innovation Journal, vol. 10, pp. 10451054, 2021.
[9] H. S. Tuli, S. S. Sandhu, and A. K. Sharma, Pharmacological and therapeutic potential of Cordyceps with special reference to
Cordycepin, 3 Biotech, vol. 4, no. 1, pp. 112, Feb. 2014, doi: 10.1007/s13205-013-0121-9.
[10] D. Deshpande, S. Jadhav, R. Chounde, T. Kachare, K. Bhale, and P. Waso, A survey on the role of IoT in agriculture for smart
farming, in 1st IEEE International Conference on Artificial Intelligence and Machine Vision, 2021, vol. 7, pp. 156237156271,
doi: 10.1109/AIMV53313.2021.9670901.
[11] O. J. Olatunji et al., Neuroprotective effects of adenosine isolated from Cordyceps cicadae against oxidative and ER stress
damages induced by glutamate in PC12 cells, Environmental toxicology and pharmacology, vol. 44, pp. 5361, 2016.
[12] O. J. Olatunji et al., Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative
damage, Carbohydrate Polymers, vol. 153, pp. 187195, 2016, doi: 10.1016/j.carbpol.2016.06.108.
Int J Elec & Comp Eng ISSN: 2088-8708
An Internet of things enabled framework to monitor the lifecycle of (Minakshi Memoria)
1149
[13] M. Joshi, A. Sagar, S. Kanwar, and S. Singh, Anticancer, antibacterial and antioxidant activities of Cordyceps militaris, Indian
Journal of Experimental Biology (IJEB), vol. 57, no. 1, pp. 1520, 2019.
[14] Mamta et al., Phytochemical and antimicrobial activities of Himalayan Cordyceps sinensis (Berk.) Sacc, Indian Journal of
Experimental Biology, vol. 53, no. 1, pp. 3643, 2015.
[15] C. P. Chiu et al., Anti-inflammatory cerebrosides from cultivated cordyceps militaris, Journal of Agricultural and Food
Chemistry, vol. 64, no. 7, pp. 15401548, 2016, doi: 10.1021/acs.jafc.5b05931.
[16] Y.-S. Kim et al., Anti-inflammatory effect of the extract from fermented Asterina pectinifera with Cordyceps militaris mycelia in
LPS-induced RAW264. 7 macrophages, Food Science and Biotechnology, vol. 26, no. 6, pp. 16331640, 2017.
[17] R. M. Kunwar, L. Mahat, R. P. Acharya, and R. W. Bussmann, Medicinal plants, traditional medicine, markets and management
in far-west Nepal, Journal of Ethnobiology and Ethnomedicine, vol. 9, no. 1, Dec. 2013, doi: 10.1186/1746-4269-9-24.
[18] R. K. Arora, Cordyceps sinensis (Berk) Sacc.-an entomophagous medicinal fungus- a review, International Journal of Recent
Advances in Multidisciplinary Research, vol. 2, no. 1, pp. 161170, 2015.
[19] J. Zhao, J. Xie, L. Y. Wang, and S. P. Li, Advanced development in chemical analysis of Cordyceps, Journal of
Pharmaceutical and Biomedical Analysis, vol. 87, pp. 271289, Jan. 2014, doi: 10.1016/j.jpba.2013.04.025.
[20] M. E. Valverde, T. Hernández-Pérez, and O. Paredes-López, Edible mushrooms: improving human health and promoting quality
life, International Journal of Microbiology, vol. 2015, pp. 114, 2015, doi: 10.1155/2015/376387.
[21] W. Yalin, O. Ishurd, S. Cuirong, and P. Yuanjiang, “Structure analysis and antitumor activity of (1→ 3)-β-D-glucans
(cordyglucans) from the mycelia of Cordyceps sinensis, Planta medica, vol. 71, no. 4, pp. 381384, 2005.
[22] Y. K. Rao, S. H. Fang, and Y. M. Tzeng, Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of
Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts, Journal of Ethnopharmacology,
vol. 114, no. 1, pp. 7885, 2007, doi: 10.1016/j.jep.2007.07.028.
[23] W.-F. Chiou, P.-C. Chang, C.-J. Chou, and C.-F. Chen, Protein constituent contributes to the hypotensive and vasorelaxant
acttvtties of cordyceps sinensis, Life Sciences, vol. 66, no. 14, pp. 13691376, Feb. 2000, doi: 10.1016/S0024-3205(00)00445-8.
[24] C. Xun et al., Radiation mitigation effect of cultured mushroom Fungus hirsutella sinensis (CorImmune) isolated from a
Chinese/Tibetan herbal preparationCordyceps sinensis, International Journal of Radiation Biology, vol. 84, no. 2, pp. 139149,
2008.
[25] R. R. M. Paterson, Cordyceps a traditional Chinese medicine and another fungal therapeutic biofactory?, Phytochemistry,
vol. 69, no. 7, pp. 14691495, May 2008, doi: 10.1016/j.phytochem.2008.01.027.
[26] T. Kawanishi, Y. Ikeda-Dantsuji, and A. Nagayama, Effects of two basidiomycete species on interleukin 1 and interleukin 2
production by macrophage and T cell lines, Immunobiology, vol. 215, no. 7, pp. 516520, Jul. 2010, doi:
10.1016/j.imbio.2009.10.005.
[27] A. K. Panda and K. C. Swain, Traditional uses and medicinal potential of Cordyceps sinensis of Sikkim, Journal of Ayurveda
and integrative medicine, vol. 2, no. 1, 2011.
[28] E. B. Mains, Species of cordyceps on spiders, Bulletin of the Torrey Botanical Club, vol. 81, no. 6, pp. 492500, 1954, doi:
10.2307/2481945.
[29] Y.-J. Tsai, L.-C. Lin, and T.-H. Tsai, Pharmacokinetics of adenosine and cordycepin, a bioactive constituent of cordyceps
sinensis in rat, Journal of Agricultural and Food Chemistry, vol. 58, no. 8, pp. 46384643, Apr. 2010, doi: 10.1021/jf100269g.
[30] L. Deshmukh, R. Singh, and S. S. Sandhu, Far ranging antimicrobial and free radical scavenging activity of Himalayan soft gold
mushroom; Cordyceps sp., in Biotechnology and Biological Sciences, CRC Press, 2019, pp. 297302.
[31] A. Uhrinová and N. Poľančíková, “Antioxidant activity of the Fungus Cordyceps sinensis grown on two different media, Folia
Veterinaria, vol. 62, no. 3, pp. 6873, Sep. 2018, doi: 10.2478/fv-2018-0030.
[32] H. C. Wu et al., Radical scavenging and antiproliferative effects of cordycepin-rich ethanol extract from brown ricecultivated
cordyceps militaris (Ascomycetes) mycelium on breast cancer cell lines, International Journal of Medicinal Mushrooms, vol. 21,
no. 7, pp. 657669, 2019, doi: 10.1615/IntJMedMushrooms.2019031138.
[33] P. Staments and J. S. Chilton, The mushroom cultivator. Agarikon Press, 1983.
[34] B. Q. Lin and S. P. Li, Cordyceps as an herbal drug, in Herbal Medicine: Biomolecular and Clinical Aspects: Second Edition,
vol. 5, 2011, pp. 73105.
[35] S. K. Singh and R. Pathak, Cultivation, conservation and medicinal significance of macrofungi, in Fungi and their Role in
Sustainable Development: Current Perspectives, Singapore: Springer Singapore, 2018, pp. 322.
[36] V. P. Sharma, S. K. Annepu, Y. Gautam, M. Singh, and S. Kamal, Status of mushroom production in India, Mushroom
Research, vol. 26, no. 2, pp. 111120, 2017.
[37] P. Sihombing, T. P. Astuti, Herriyance, and D. Sitompul, Microcontroller based automatic temperature control for oyster
mushroom plants, Journal of Physics: Conference Series, vol. 978, no. 1, Mar. 2018, doi: 10.1088/1742-6596/978/1/012031.
[38] A. Marzuki and S. Y. Ying, Environmental monitoring and controlling system for mushroom farm with online interface,
International Journal of Computer Science and Information Technology (IJCSIT), vol. 9, 2017.
BIOGRAPHIES OF AUTHORS
Minakshi Memoria is currently working as HOD-CSE in Uttaranchal Institute
of Technology, Uttaranchal University, Dehradun. She has done her Ph.D. in Computer
Science and Engineering from Gyan Vihar Univeristy, Jaipur, Rajasthan. She has more than
16 years of teaching experience and also has various patents in international and national
government patents and published various papers in various national and international
journals and conferences. Her area of research includes grid computing, cloud computing,
automata, advanced algorithms, artificial intelligence, and distributed computing. She can be
contacted at minakshimemoria@gmail.com.
ISSN: 2088-8708
Int J Elec & Comp Eng, Vol. 13, No. 1, February 2023: 1142-1151
1150
Sanjeev Kumar Shah is currently working as a professor. His area of research
is microwave, artificial intelligence, and machine learning. He is also an associate member of
the Institution of Electronics and Telecommunication Engineers. He has published 25
scientific papers and 35 patents. He can be contacted at sanjeevkshah19@gmail.com.
Harishchander Anandaram is currently working as assistant professor, Center
for Computational Engineering and Networking (CEN), Coimbatore Campus. His research
area is metabolic engineering, bioinformatics, functional genomics. He can be contacted at
a_harishchander@cb.amrita.edu.
Anooja Ali currently working as assistant professor at REVA University,
Bangalore with 14 years of experience in teaching. He has completed Ph.D. from REVA
University in algorithms for analysis of protein sequences in cervical cancer. To her credit,
she has 16 journal publications, 7 conference papers, 5 copyrights, and 3 Indian patent. Her
areas of interest include machine learning and bioinformatics. She can be contacted at
Anooja.ali@reva.edu.in.
Kapil Joshi currently working as an assistant professor in the Department of
Computer Science & Engineering (CSE) at Uttaranchal Institute of Technology (UIT),
Uttaranchal University, Dehradun, India. He has around 8 years of academic experience in
different institutions/companies. His areas of interest include operating system, computer
networks, web technology, data structure, and Java. He can be contacted at email:
Kapilengg0509@gmail.com.
Parag Verma currently working as an assistant professor in the Department of
Computer Science & Engineering (CSE) at Uttaranchal Institute of Technology (UIT),
Uttaranchal University, Dehradun with more than nine years in the field of industry and
academics. He has more than 25 international papers in reputed conferences and journals. His
research interests include IoT, healthcare, and image processing-based analysis using
distributed computing platforms. He can be contacted at parag_verma@yahoo.com.
Int J Elec & Comp Eng ISSN: 2088-8708
An Internet of things enabled framework to monitor the lifecycle of (Minakshi Memoria)
1151
Rajesh Singh is currently associated with Uttaranchal University as a professor
and director of R&I and post doc fellow at Department of Project Management, Universidad
Internacional Iberoamericana, Campeche, C.P. 24560, México with more than seventeen
years of experience in academics. He has been featured among top ten inventors for ten years
2010-2020, by Clarivate Analytics in Indias Innovation Synopsis in March 2021 for filing
three hundred and fifty-eight patents. He has twelve patents grant (8 Australian and 4 Indian
patents), 5 PCT and published more than hundred research papers in SCI/Scopus journals. He
can be contacted at drrajeshsingh004@gmail.com.
Anita Gehlot is currently associated with Uttaranchal University as
a professor & head (R&D) with more than fifteen years of experience in academics. She has
been featured among top ten inventors for ten years 2010-2020, by Clarivate Analytics in
Indias Innovation Synopsis in March 2021 for filing two hundred and sixty-three patents.
She has published more than 80 research papers in SCI/Scopus journals. She has twelve
patents grant (8 Australian and 4 Indian patents), 5 PCT. She can be contacted at email:
dranitagehlot@gmail.com.
Shaik Vaseem Akram is currently working as an assistant professor at
Uttaranchal University, Dehradun. He has published 26 articles in SCI/Scopus. He has
published more than 160 patents in which 5 technology transfer. He can be contacted at
vaseemakram5491@gmail.com.
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The yield and efficacy of bioactive compounds from Cordyceps militaris fruiting bodies and its fermented grains usually vary with the strain used. In this study, we compared the antiproliferative, apoptotic, and antioxidative properties of ethanolic extracts of fruiting bodies and solid-stated fermented rice (FRE) from two wild-type strains of C. militaris applied to human breast cancer cell lines. We observed that FRE of the Zhangzhou strain (FRE-Z) produced a high level of cordycepin and exhibited comprehensive in vitro antioxidant activity against the oxidation of 2,2-diphenyl-1-picrylhydrazyl, superoxide, and hydroxyl radicals and low-density lipoprotein. Only FRE-Z exhibited dose-dependent inhibition of cell proliferation in MCF-7 (0.7 mg/mL) and MDA-MB-231 cells (1 mg/mL) after culturing for 24 h. The antiproliferative effects of FRE-Z were associated with an early stage of apoptosis induction at 4 h of treatment with 0.5 mg/mL FRE-Z in MCF-7 cells. The antiproliferative effect was determined to occur through p53 activation but not through the release of mitochondrial apoptosis-inducing factor or caspase-9 activation for an initial culture period of 16 h. In addition to a transient increase in cellular antioxidant enzyme, Cu/Zn superoxide dismutase was identified in MCF-7 cells after 2 h of treatment with FRE-Z. Therefore, FRE-Z, which exhibits various dose- and exposure time-dependent activities, has potential application in breast cancer chemoprevention.
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Chinese cordyceps, an entity of the Chinese caterpillar fungus (Ophiocordyceps sinensis, syn. Cordyceps sinensis) that parasitizes ghost moth larvae, is one of the best known traditional Chinese medicines and is found exclusively on the Tibetan Plateau with limited natural resources. Although the fungus O. sinensis can grow on artificial substrates and the ghost moth has been successfully reared, the large-scale artificial cultivation of Chinese cordyceps has only recently been accomplished after several decades of efforts and attempts. In this article, research progress related to this breakthrough from living habitats, the life history of the fungus, its host insect, fungal isolation and culture, host larvae rearing, infection cycle of the fungus to the host, primordium induction, and fruiting body development have been reviewed. An understanding of the basic biology of O. sinensis, its host insect and the simulation of the Tibetan alpine environment resulted in the success of artificial cultivation on a large scale. Practical workshop production has reached annual yields of 2.5, 5, and 10 tons in 2014, 2015, and 2016, respectively. There was no difference in the chemical components detected between the cultivated and natural Chinese cordyceps. However, the artificial cultivation system can be controlled to avoid heavy metal contamination and results in high-quality products. Although omics studies, including genomic, transcriptomic, proteomic, and metabolomic studies, have helped to understand the biology of the fungus, the success of the artificial cultivation of the Chinese cordyceps is clearly a milestone and provides the possibility for research on the in-depth mechanisms of the interaction between the fungus and host insects and their adaptation to the harsh habitats. This cultivation will not only result in a large industry to alleviate the pressure of human demand but also protect the limited natural resources for sustainable utilization.