An experimental autoradiographic study of P32 tagged Candida albicans infection in rabbits

Source: OAI


To study the pathogenesis of experimental candidiasis in animals, rabbits
given an intratraeheal injection of P32- labelled C. albicans in a suspension.
The organ immunity of the lung has been overcome by using a massive dose of fungus.
Dissemination of the fungus into all the distant organs was rapid and uniform. This can be achieved
only by a haematogenous route.
The autoradiography method used for (racing the isotope labelled C. aibicans showed that
apart from corroborating histopathoiogical lesions, and the culture of the fungus in tissues; the
autoradiography was positive during the last few days of the experiment, at which time all the
lesions were healed in the tissues and the fungus was negative by culture, and by histopathology,
This is an added method to identify Candida albians in tissues when conventional methods of
culture and histopathology of tissue have failed.

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    • "Indeed, Group D showed high cfu counts in the lungs compared to other organs. Our results confirm those previously reported that emphasize the efficiency of the lungs in retaining and eliminating viable Candida (blastospores and pseudohyphae) in in-vivo experimental infection models (Walbaum and Dujardin, 1985; Damodaran et al., 1973). This also supports the absence of pneumonitis in our experimental model. "
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    ABSTRACT: We investigated the effect of dietary carbohydrate supplementation on Candida albicans colonization using an animal model and a Candida strain with both high phospholipase and proteinase activity. Normal and immunosuppressed mice were inoculated intraperitonially with C. albicans and were allowed free access to drinking water supplemented or not with glucose. Blood and different organs including liver, spleen, lung and kidney were aseptically collected every 72 h post-infection. The presence and the growth of C. albicans in blood and each organ were investigated. We also performed histopathologic investigations on each organ to assess tissue structure, the presence of C. albicans, and its form (blastospore or hyphae). The results showed that on the third day post-inoculation, the cfu of C. albicans per organ was significantly higher in mice inoculated by C. albicans and receiving the glucose as supplement (433 cfu/liver) comparatively to the group receiving C. albicans only (140 cfu/liver). Histological analysis revealed the presence of Candida cells in blastospore and hyphal form, particularly in the Candida-infected glucose-supplemented mice whose livers displayed oedema and leukocyte infiltration with a high density of polymorphonuclear cells. Overall results indicate that dietary glucose supplementation leads to higher rates of Candida growth and invasion. This suggests that glucose restriction could be a possible way to control C. albicans pathogenesis in vivo.
    Full-text · Article · Sep 2011 · African journal of microbiology research