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Accessible Bioactive Compounds and Health Benefits of Amla (Emblica officinalis)

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Copyright © September, 2022; Current Agriculture Trends 9
Available online at
www.currentagriculturetrends.vitalbiotech.org
ISSN (E): 2583 1933
Curr. Agri.Tren.:e-Newsletter, (2022) 1(9), 9-12
Article ID: 153
Accessible Bioactive Compounds and Health Benefits of Amla
(Emblica officinalis)
Mukesh Kumar Sahu*,
Arpan Buragohain,
Nilotpol Shankarison,
Suprakash Sau
Assam Agricultural University,
Jorhat-13
INTRODUCTION
Amla has found its important position in the Indian
indigenous system of medicine like Ayurveda since a very
long period of time. It is widely grown in tropical and sub-
tropical parts of the world viz. India, Pakistan, China, South
East Asia and Iran (Vasudevan and Parle, 2007). Amla has
an important role in ayurvedic medicine for treatment of
anxiety and other problems related to digestion and skin,
improves, anemic conditions, improve functioning of liver
and has a positive effect on cardio-vascular system of body
(Asmawi, 1992). Presence of polyphenols, Vitamin-C amino
acids, minerals and other bioactive compounds makes the
amla fruit a natural source of anti-oxidant. It is small to
medium sized deciduous tree and the height of plant ranges
from 8 to 18 meter with light grey bark, leaves are simple
and light green, flowers are greenish yellow. Fruits have 6
vertical furrows and are globose, fleshy and pale yellow in
colour (Parmar and Kaushal, 1982). Amal is frequently used
for the Industrial processing for the preparation of various
kinds of products in the Industry at large scale (Pareek,
2011). Most of the products of amla like Jam, pickle,
Chawanprash, candy, digestive powder etc. are available in
the marker as Ayurveda product (Singh, 2003).
Figure 1. Fresh fruit of the Amla
Article History
Received: 27.
08.2022
Revised: 10.
09.2022
Accepted: 14.
09.2022
This article is published under the
terms of the Creative Commons
Attribution License 4.0
.
*Corresponding Author
Mukesh Kumar Sahu*
Copyright © September, 2022; Current Agriculture Trends 10
Fruiting season:
As amla is a deciduous tree, new
shoots starts emergence during the start of
April. The fruiting period lies between
December to March and sometimes extending
up to April. The tree of amla is heavy bearer
and fruits generally remain free from the
attack of birds and insects. The harvesting of
the fruit gives maximum yield during January
to March.
Nutrient composition in Aonla
Amla serves as source of
carbohydrates, fibres, proteins and minerals
like Iron, Calcium and Phosphorous. It is rich
source of Vitamin-C, Emblicinin A and B
which have anti-oxidant properties (Barthakur
and Arnold, 1991). The chemical composition
and nutrients availability in amla fruit is
mentioned in table 1.
Table 1. Nutritional composition of Amla (Shwetha and Krishna, 2014)
Quantity
81.2 %
0.5 %
0.1 %
3.4 %
14.1 %
0.05 %
0.02 %
12 µg/g
6 mg/g
Bioactive compounds in Aonla
Amla has been found to be a rich
source of different types of bioactive
compounds. Polyphenols comprise the main
group of secondary metabolites wherein
several compounds belonging to phenolic
acids, flavonoids, tannins, other phenolics and
derivatives compounds have been reported in
different studies (Liu, et al., 2008). The amla
fruit also includes numerous bioactive
components including isostrictiniin, ellagic
acid, apigenin, chebulinic acid, quercetin,
gallic acid, chebulagic acid. The tannins also
found in the fruit extract of amla
arepedunculagin, emlicanin A, phyllaemblicin
B, emblicanin B and punigluconine . 100 g of
edible fruit have been reported to be 470680
mg of Vit. C (Bansal, et al., 2015). A
composite list of bioactive coumpounds
present in amla has been given in table 2.
Table2: Classes of chemical compounds in amla
Classes
Compounds
Alkaloid
Phyllantine, phyllantidine, zeatin, zeatin nucleotide, zeatin rioside
Benzoid
chebulic acid, chebulinic acid, chebulagic acid, gallic acid, ellagic acid, amlaic
acid, 3-6-di-O-galloyl-glucose, ethyl gallate, b-glucogallin, 1,6,di-O-galloyl-b-
D-glucose, putranjivan A, digallic acid, phyllemblic acid, emlicol, galacteric
acid
Diterpene
gibberellin A-1, gibberellin A-3, gibberellin A-4, giberellin A-9
Triterpene
lupeol
Flavonoid
Leucodelphinidin, kaempherol, kaempherol-3-glucoside, rutin
Quercetin, kaempherol-3-O- β -D-glucoside, quercetin -3-O- β -D glucoside
Furanolactone
Ascorbic acid
Sterol
β-sitosterol
Copyright © September, 2022; Current Agriculture Trends 11
Health Benefits
Apart from use of amla in the
preparation of ayurvedic medicines, it plays a
significant role in curing various ailments like
liver diseases, digestion related problems,
treatment of diabetes and cancer. In several
studies the anticancer potential of six phenolic
compounds isolated from Amla fruit by in
vitro proliferation assay (Adil, et al., 2010).
MTT method was used to study the effects of
these compounds on splenocyte proliferation
and the cytotoxicity to both human breast
cancer cell (MCF-7) and human embryonic
lung fibroblast cell (HELF) (Krishnaveni and
Mirunalini, 2012). Amla promotes immune
system functioning, a rich source of vitamin-C,
antioxidants, increases hair growth, etc
(Hussain, et al., 2021).
CONCLUSION
Amla is the well-known fruits of the tropical
and subtropical region of the world, mostly
planted for the fruit production. Amla is the
potent source of vitamin C that can prevent
cellular damage of our body. Minerals and
vitamins found in amla fruit acts as antioxidant
for human body and gives glow to the skin.
Amla juice is well-known for health hair
growth also prevent hair fall (Yokozawa, et al.,
2007). In several studies, amla fruit have
found to be one of the important fruit and play
crucial role in the control of cancerous cell
(Khan, 2009). It may also protect our boy from
oxidative damage. High blood pressure and
cardio-vascular diseases can also be control
using fresh fruit and by-product of the amla. In
several studies related to Ayurveda, amla fruits
have been found to be one of the potent
immunity boosters and provide strength to the
peoples for optimizing the immunity level up
to certain level.
REFERENCES
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ResearchGate has not been able to resolve any citations for this publication.
Chapter
: This chapter presents and reviews the medicinal, chemical and physical properties of aonla fruit and its wide range of uses. Fruit growth and development, preharvest factors affecting postharvest quality, postharvest handling, physiology, postharvest disorders, insect pests and diseases of aonla fruit are described and discussed. Finally, information on processing and food, medicinal and cosmetic processed products of aonla fruit is provided.
Article
Although numerous herbs have been shown to have potent biological activity, edibleherbs are more attractive in traditional medicine. Emblica officinalis, generally referredto as amla, is of particular interest due its enormous medicinal value. Amla is generallyconsidered safe (GRAS) by the US Food and Drug Administration (FDA) and has beenauthenticated by many pharmaceutical companies. In this chapter, we will discuss theorigin of amla and its availability throughout the world as well as the chemicalconstituents of each part of the plant. We will also summarize the therapeutic efficaciesof amla, including its anticancer, antihyperglycemic, antioxidant, cardioprotective,antipyretic, analgesic, antihyperchloresterolemic, and cytoprotective properties. Insummary, inclusion of amla in a normal diet can alleviate a number of health hazards.
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Emblica officinalis fruit (EO), commonly known as Amla is a reputed traditional medicine and functional food used in Indian subcontinent. It has long been used in Indian folk medicine to treat liver diseases, stomach ulcers, inflammatory diseases, metabolic disorders, geriatric complaints, skin disorders and beauty care. Recently, it has been shown to promote pro-collagen content and inhibit matrix metalloproteinase levels in skin fibroblast. The aim of the present study was to investigate the efficacy of EO to inhibit UVB-induced photo-aging in human skin fibroblasts. Mitochondrial activity of human skin fibroblasts was measured by MTT-assay. Quantifications of pro-collagen 1 and matrix metalloproteinase 1 (MMP-1) release were performed by immunoassay techniques. Hyaluronidase inhibition assay was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid. Cell cycle analysis was performed by flowcytometry using propidium iodide. EO stimulated, the otherwise UVB inhibited cellular proliferation and protected pro-collagen 1 against UVB-induced depletion via inhibition of UVB-induced MMP-1 in skin fibroblasts (10-40 μg/mL, p>0.001). EO exhibited inhibitory activity of hyaluronidase (10-40 μg/mL, p>0.001). Treatment with EO also prevented UVB disturbed cell cycle to normal phase. The results of the present study suggests that EO effectively inhibits UVB-induced photo-aging in human skin fibroblast via its strong ROS scavenging ability and its therapeutic and cosmetic applications remain to be explored.
Article
Emblica officinalis Gaertn, a tree growing in subtropical and tropical parts of China, India, Indonesia and the Malay Peninsula, has been used for anti-inflammatory and antipyretic treatments of rural populations in these areas. In the present study, we examined the effects of Emblica officinalis extracts on carrageenan- and dextran-induced rat hind paw oedema. Anti-inflammatory activity was found in the water fraction of methanol extract of the plant leaves. The effects of the same fraction were tested on the synthesis of mediators of inflammation such as leukotriene B4 (LTB4), platelet-activating factor (PAF) and thromboxane B2 (TXB2), and on LTB4- and N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP)-induced migration of human polymorphonuclear leucocytes (PMNs) in-vitro. The water fraction of the methanol extract inhibited migration of human PMNs in relatively low concentrations. It did not inhibit LTB4 or PAF synthesis in human PMNs or TXB2 synthesis in human platelets during clotting, suggesting that the mechanism of the anti-inflammatory action found in the rat paw model does not involve inhibition of the synthesis of the measured lipid mediators.
Article
Ayurveda means "the science of life". Ayur means "life" and Veda means "knowledge or science". It is the oldest medical system in the world. Its origins can be traced as far back as 4500 BC, to four ancient books of knowledge, (the "Vedas") and it is still officially recognized by the government of India. The present study was aimed at investigating the effects of Anwala churna (Emblica officinalis Gaertn.), an Ayurvedic preparation on memory, total serum cholesterol levels and brain cholinesterase activity in mice. Anwala churna was administered orally in three doses (50, 100 and 200 mg/kg) for fifteen days to different groups of young and aged mice. Elevated plus maze and passive avoidance apparatus served as the exteroceptive behavioral models for testing memory. Diazepam-, scopolamine- and ageing-induced amnesia served as the interoceptive behavioral models. Total serum cholesterol levels and brain cholinesterase activity also estimated. Anwala churna (50, 100 and 200 mg/kg, p.o.) produced a dose-dependent improvement in memory scores of young and aged mice. Furthermore, it reversed the amnesia induced by scopolamine (0.4 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.). Interestingly, brain cholinesterase activity and total cholesterol levels were reduced by Anwala churna administered orally for 15 days. Anwala churna may prove to be a useful remedy for the management of Alzheimer's disease on account of its multifarious beneficial effects such as, memory improving property, cholesterol lowering property and anticholinesterase activity.
Article
To investigate the effects of amla on renal dysfunction involved in oxidative stress during the aging process, we employed young (2 months old) and aged (13 months old) male rats and administered SunAmla (Taiyo Kagaku Co., Ltd., Japan) or an ethyl acetate (EtOAc) extract of amla, a polyphenol-rich fraction, at a dose of 40 or 10 mg/kg body weight/day for 100 days. The administration of SunAmla or EtOAc extract of amla reduced the elevated levels of serum creatinine and urea nitrogen in the aged rats. In addition, the tail arterial blood pressure was markedly elevated in aged control rats as compared with young rats, while the systolic blood pressure was significantly decreased by the administration of SunAmla or EtOAc extract of amla. Furthermore, the oral administration of SunAmla or EtOAc extract of amla significantly reduced thiobarbituric acid-reactive substance levels of serum, renal homogenate, and mitochondria in aged rats, suggesting that amla would ameliorate oxidative stress under aging. The increases of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expression in the aorta of aging rats were also significantly suppressed by SunAmla extract or EtOAc extract of amla, respectively. Moreover, the elevated expression level of bax, a proapoptotic protein, was significantly decreased after oral administration of SunAmla or EtOAc extract of amla. However, the level of bcl-2, an antiapoptotic protein, did not show any difference among the groups. The expressions of renal nuclear factor-kappaB (NF-kappaB), inhibitory kappaB in cytoplasm, iNOS, and COX-2 protein levels were also increased with aging. However, SunAmla or EtOAc extract of amla reduced the iNOS and COX-2 expression levels by inhibiting NF-kappaB activation in the aged rats. These results indicate that amla would be a very useful antioxidant for the prevention of age-related renal disease.
Emblica officinalis. In wild fruits
  • C Parmar
  • M K Kaushal
Parmar, C. and Kaushal, M.K. (1982). Emblica officinalis. In wild fruits. New Delhi;