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ecancer 2022, 16:1414; www.ecancer.org; DOI: https://doi.org/10.3332/ecancer.2022.1414 1
Research
Leptomeningeal metastasis from non-small cell lung cancer—
a post-hoc analysis from four randomised clinical trials
Vijay Patil1†, Vanita Noronha1†, Dilip Harindran Vallathol1†, Nandini Menon1, Abhishek Mahajan2, Amit Janu2, Nilendu Purandare3 and
Kumar Prabhash1
1Department of Medical Oncology, Tata Memorial Centre, HBNI, Mumbai 400012, India
2Department of Radiology, Tata Memorial Centre, HBNI, Mumbai 400012, India
3Department of Nuclear Medicine, Tata Memorial Centre, HBNI, Mumbai 400012, India
†Both contributed equally.
Correspondence to: Kumar Prabhash
Email: kumarprabhashtmh@gmail.com
ecancer 2022, 16:1414
https://doi.org/10.3332/ecancer.2022.1414
Published: 16/06/2022
Received: 21/08/2021
Publication costs for this article were supported by
ecancer (UK Charity number 1176307).
Copyright: © the authors; licensee
ecancermedicalscience. This is an Open Access
article distributed under the terms of the
Creative Commons Attribution License (http://
creativecommons.org/licenses/by/4.0), which
permits unrestricted use, distribution, and
reproduction in any medium, provided the original
work is properly cited.
Abstract
Background: Leptomeningeal metastasis (LMM) from non-small cell lung cancer (NSCLC)
is often an underdiagnosed entity, has a dismal prognosis and has very limited data from
low- and middle-income countries.
Methods: A single-centre study, which included 1148 adult patients diagnosed as
NSCLC, with Eastern Oncology Cooperative Group performance status 0–2, as identi-
fied from data of four prospective randomised controlled trials. Two trials included
patients who had epidermal growth factor sensitive mutations (CTRI/2015/08/006113
and CTRI/2016/08/007149) and the other two included squamous cell carcinoma
(CTRI/2013/02/003422) and adenocarcinoma patients (CTRI/2014/08/00484). The
key objectives were to estimate the incidence, risk factors, time to development and
outcomes for LMM.
Results: Out of 1148 patients, 36 patients (0.031%; 95%CI: 0.022–0.043) developed
leptomeningeal metastasis. In these patients, median time to development of LM was
14.92 months (interquartile range: 7.7–21.84). Among the tested factors, the presence
of brain metastasis was the only statistically significant risk factor associated with the
development of LMM (p-value = 0.035). The median overall survival (OS) after the devel-
opment of LM was 61 days (95%CI: 38.95–83.05). The median OS in driver mutated
patients was 66 days (95% CI: 14.74–117.26) versus 51 days (95% CI: 14.5–87.5)
(p-value = 0.201) in non-driver mutated patients. Only 6 (19.4%) out of 31 epidermal
growth factor receptor-mutated patients received osimertinib. Patients treated with
osimertinib had a median OS of 245 days (95% CI: 215.48–274.52) versus 52 days
(95% CI: 22.62–81.38) for those without (p-value = 0.327).
Conclusion: The incidence of LMM is low in the Indian population. In our study, there
was no single factor which impacted survival in patients who developed LMM. This sug-
gests that the overall prognosis is poor in patients with LMM where access to newer
therapeutic modalities is limited.
Keywords: leptomeningeal metastasis, non-small cell lung cancer, incidence, survival
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ecancer 2022, 16:1414; www.ecancer.org; DOI: https://doi.org/10.3332/ecancer.2022.1414 2
Introducon
Leptomeningeal metastasis (LMM) is a rare and underdiagnosed entity in extracranial solid tumours [1, 2]. It often occurs in the setting of
a progression of systemic disease. The estimated incidence of LMM in solid tumours (excluding central nervous system tumours) is around
5%–10% [3]. The treatment of LMM has also evolved, but the prognosis of LMM remains dismal when compared to other sites of metas-
tasis [4]. The two commonest extracranial solid tumour sites leading to LMM are breast carcinoma and non-small cell lung cancer (NSCLC)
[3]. The improvement in diagnostic modalities and newer modalities of treatment of malignancies has led to an increase in the diagnosis of
LMM [5, 6].
The occurrence of LMM is particularly increased in patients with driver mutations as a result of their improved outcomes with targeted
therapies and better extracranial disease control [5, 6]. Previously reported studies by Yang et al [6] and Liao et al [7] in a similar setting
showed higher rates of LMM among epidermal growth factor receptor (EGFR)-mutated patients when compared to mutation negative
patients. Yang et al [6] showed a 9.4% incidence of LMM among EGFR-mutated patients when compared to 1.7% in negative ones; Liao et
al [7] reported 212 patients (3.8%) with LMM in NSCLC of which 75 patients harboured EGFR mutations. The mechanisms responsible for
the higher frequency of LMM in patients with driver mutations may be multifactorial. Longer survival and insufficient penetration of tyrosine
kinase inhibitors (TKIs) into the CSF are likely to be the main explanations. With the application of TKIs, overall survival (OS) of metastatic
NSCLC with driver mutations was improved in comparison to the OS of patients with wild-type. Moreover, the ratio of the CSF to plasma
concentration of TKIs is low on account of incomplete penetration of the blood–brain barrier [8]. Hence, insufficient levels of TKIs, which
result in continuing lower stimulation of tumour cells, may favour resistance and pave the way for LMM. Even though these are postulated,
the exact mechanism has not yet been elucidated. Globally, the improvement in treatment of malignancies, with the entry of novel therapies
and higher generation of targeted agents with better central nervous system penetration, has improved the median survival in LMM from
1–3 months to 3–11 months [5, 6]. However, access to these novel agents is limited in low- and middle-income countries (LMICs). There is
very limited literature regarding the incidence, presentation symptoms, diagnostic modalities, treatment and outcomes of LMM with these
novel therapies from LMICs. Over the last 5 years, we have reported four randomised studies in NSCLC from India [9–12]. These studies
have contributed towards the improvement in outcomes of NSCLC. In view of the limited data on LMM from NSCLC from LMIC, we carried
out this post-hoc analysis on the data obtained from these four prospective randomised studies, with a key objective to estimate the median
OS in patients who develop LMM after diagnosis of NSCLC. The other objectives were to estimate the incidence of LMM, risk factors, clinical
presentation and pattern of treatment.
Materials and methods
Paent selecon
This was a post-hoc analysis on the data collected from the four prospective randomised controlled trials conducted on NSCLC patients at a
single centre in the last 10 years. All the four studies have been published and the detailed study protocols are available online.
These trials included the following:
1. PatientswhohadEGFRsensitisingmutationsenrolledina1:1fashioneitherinthepemetrexedversusgefitinibtrial(Pem-Gefstudy,
February 2012 and April 2016) [9] or gefitinib versus gefitinib plus pemetrexed and carboplatin trial (Gef versus Gef+C study, August
2016 and August 2018) [10]. The primary end point was progression-free survival (PFS) with OS being a key secondary end point in
both studies.
2. Patientswhowerediagnosedwithsquamouscellcarcinomaofthelungswereenrolledina1:1fashioninthelow-dosegemcitabine
with platinum versus standard-dose gemcitabine with the platinum trial (May 2013–March 2018) [11]. OS was the primary end point.
3. Patientswithadenocarcinomawhowereenrolledinthepemetrexedversuserlotinibmaintenancetrial(November2014–March2017)
with quality of life being the primary end point [12].
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ecancer 2022, 16:1414; www.ecancer.org; DOI: https://doi.org/10.3332/ecancer.2022.1414 3
Data collecon
The data were extracted from the trial database of the four aforementioned prospective trials. The demographic details at enrollment in
the trial and at LMM, driver mutation status, brain metastasis status and treatment details were extracted. In addition, clinical presentation,
diagnostic details, treatment, response assessment and the outcomes for LMM were extracted.
Outcome variable
The occurrence of LMM was defined as positive cerebrospinal fluid (CSF) cytology or CSF sampling performed only when symptomatic.
Contrast-enhanced magnetic resonance imaging (MRI) with gadolinium contrast features was consistent with the diagnosis of LMM [11]. CSF
cytology was carried out in 17 patients and MRI was carried out in 27 patients. CSF cytology was especially carried out in all symptomatic
patients who were eligible for intrathecal therapy. These neuroimaging features were the unequivocal presence of leptomeningeal nodules or
enhancement. The response to LMM was assessed in accordance with the RANO working group proposal [12]. OS was defined as the time
in months from the date of diagnosis of LMM to the date of death.
Stascal analysis
SPSS version 17 and RStudio version 3.1 were used for analysis. Descriptive statistics were carried out. The incidence of LMM was calculated
using a competing risk analysis. Death due to any other cause before developing LMM was considered as competing. The factors affecting
LMM development were identified using binary logistic regression analysis. The factors tested were age, performance status on the Eastern
Oncology Cooperative Group (ECOG PS) scale, driver mutation status and previous brain metastasis. OS was estimated using the Kaplan–
Meier analysis. COX regression analysis was used to identify factors affecting OS. p-value of 0.05 was considered significant.
Results
Incidence of LMM
From our database of 1148 patients, we identified 36 (0.031%; 95%CI: 0.022–0.043) patients of NSCLC who developed LMM in the
past decade. The incidence of LMM is shown in Figure 1. The median time to develop LMM was 14.92 months (interquartile range (IQR):
7.7–21.84). The risk factors for development of LMM are shown in Table 1.
Baseline characteriscs in paents with LMM
Data on baseline patient characteristics at development of LMM are given in Table 2. There were 22 males (61.1%) and 14 females (38.9%)
intheanalysis.Therewere23patients(63.9%)whowerebelowtheageof60(non-elderlygroup)and13(36.1%)were≥60years(elderly
group). Twenty-four of the 36 patients (66.7%) had developed LMM after one line of therapy, while 6 patients (16.7%) received 3 or more
lines of therapy.
Clinical features
Data on symptoms at presentation were available for 31 out of 36 patients (86.1%). Majority of the patients (15 of the 31 evaluable patients
(48.4%)) presented with symptoms of altered higher mental functions or seizures. Full data on symptoms at presentation is presented in
Table 3. The diagnosis of LMM was made by CSF analysis alone in 10 patients (27.8%), while imaging was performed in 18 patients (50%).
Diagnosis by both CSF analysis and imaging was made in 8 patients (22.2%). Only 1 of the 18 patients (5.5%) who were diagnosed by CSF
analysis required 2 lumbar punctures. Systemic progression was present in 20 out of 36 patients (55.6%) of NSCLC at the time of diagnosis
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ecancer 2022, 16:1414; www.ecancer.org; DOI: https://doi.org/10.3332/ecancer.2022.1414 4
of LMM out of which 10 patients (20%) had brain progression. The median time for the development of LMM was 14.92 months (IQR:
7.7–21.84). Out of the 36 patients, 10 patients (27.8%) had brain progression or new-onset brain metastasis. The details of the diagnosis of
LMM are incorporated and presented in Table 3.
Figure 1. Incidence of leptomeningeal metastasis in the whole cohort. The green line depicts the incidence of leptomeningeal metastasis and the red line
depicts the occurrence of a compeng event – death.
Table 1. Factors predicting the development of leptomeningeal metastasis.
Variable Variable factors Leptomeningeal metastasis (%) OR (95%CI) p-value
Age Elderly (n = 439) 12 (2.7) 0.89 (0.44–1.8) 0.745
Non-elderly (n = 709) 24 (3.4) Reference
Gender Male (n = 744) 23 (3.1) 0.567 (0.283–1.135) 0.109
Female (n = 404) 13 (3.2) Reference
ECOG PS ECOG PS 0-1 (n = 1,033) 30 (2.9) 0.674 (0.269–1.687) 0.399
ECOG PS 2 (n = 115) 8 (5.2) Reference
Histopathology Non-squamous (n = 858) 35 (4.1%) 2.731 (0.32–23.318) 0.359
Squamous (n = 290) 1 (0.3%) Reference
Driver mutation status Present (n = 658) 31 (4.7) Reference 0.064
Absent (n = 490) 5 (1) 0.379 (0.136–1.058)
Brain metastasis Present (n = 153) 10 (6.5) Reference 0.035
Absent (n = 995) 26 (2.6) 0.45 (0.214–0.946)
ECOG PS, Eastern Oncology Cooperative Group performance status; OR, odds ratio; CI, confidence interval.
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Table 2. Patient characteristics.
Variable Value (n = 36)
Age
Elderly
Non-Elderly
13 (36%)
23 (64%)
Gender
Male
Female
22 (61%)
14 (39%)
ECOG PS
0–1
2–3
29 (81%)
7 (19%)
Histology
Squamous
Non-squamous
1 (2.8%)
35 (97.2%)
Driver Mutation
EGFR mutation
Exon 19 deletion
Exon 21 L858R
Others
31 (86.1%)
19 (61.3%)
10 (32.3%)
2 ( 6.4%)
Brain metastasis
Yes
No
9 (25%)
27 (75%)
Treatment
Twenty-three patients out of the 36 (63.9%) patients with leptomeningeal metastasis received some form of treatment. Details are pre-
sented in Table 3. Out of the 36 patients, 6 received chemotherapy (16.7%) for leptomeningeal progression. Eighteen out of 31 EGFR
mutation-positive patients (58.1%) received first-generation TKIs; 7 received high-dose TKI (erlotinib/gefitinib) (38.9%) and the rest stan-
dard dose (61.1%). Only 6 (19.4%) out of 31 EGFR-mutated patients received osimertinib. Intrathecal treatment was administered to
11 of the 36 patients (30.6%) of which 4 received triple intrathecal therapy (36.4%) (methotrexate, cytarabine and hydrocortisone) and
7 received single-agent methotrexate (63.6%). Patients with objective responses were treated with osimertinib either with standard-dose
or high-dose intrathecal therapy. Patients with a stable disease were commonly treated with a combination of intrathecal therapy, TKI
(high-dose erlotinib or gefitinib) and chemotherapy (most commonly combining TKI and intrathecal therapy). The details of the responses
to intrathecal therapy are highlighted in Table 4.
Response
Response assessment was conducted in all 23 patients (63.9%) who were treated. Response was seen in 3 patients (13%), stable disease in
11 (47.8%) and in the remaining 9 (39.2%) had a progressive or refractory disease.
Overall survival
The median OS after development of LM was 61 days (95% CI: 38.95–83.05) (Figure 2). None of the tested factors, including age (Hazard
ratio = 0.829; 95% CI: 0.383–1.792, p-value = 0.633), ECOG PS (Hazard ratio = 0.745; 95% CI: 0.306–1.813, p-value = 0.517), driver
mutation status (Hazard ratio = 2.188, 95% CI: 0.756–6.336, p-value = 0.149), presence of brain metastasis (Hazard ratio = 1.432, 95%
CI: 0.609–3.365, p-value = 0.411) or presence of positive CSF cytology (Hazard ratio = 1.026; 95% CI: 0.455–2.315, p-value = 0.95), had
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an impact on OS. The median OS in driver mutated patients was 66 days (95% CI: 14.74–117.26) versus 51 days (95% CI: 14.5–87.5)
(p-value = 0.201) in non-driver mutated patients. Among the driver mutated patients, patients treated with osimertinib had a median OS
of 245 days (95% CI: 215.48–274.52) versus 52 days (95% CI: 22.62–81.38) (p-value = 0.327) for those without.
Table 3. Clinical features, diagnosis and treatment details.
Variable Value (n = 36)
Symptoms
Altered higher mental functions(memory defects/altered
sensorium) and seizures
Cerebrovascular accident like episodes/visual disturbances
Headache and dizziness
15 (48.4%)
6 (19.4%)
10 (32.2%)
Diagnosis
CSF analysis
Imaging
CSF analysis and Imaging
10 (27.8%)
18 (50%)
8 (22.2%)
Number of other sites of metastasis
1
2
3
5 (13.8%)
21(58.3%)
10 (27.8%)
Brain metastasis prior to leptomeningeal metastasis
Yes
No 9 (25%)
27 (75%)
Number of previous lines of therapy
1
2
3
24 (66.6%)
6 (16.7%)
6 (16.7%)
Brain metastasis or progression at the time of
leptomeningeal metastasis
Yes
No
10 (27.8%)
26 (72.2%)
Intrathecal therapy
Methotrexate
Triple intrathecal therapy
11 (47.8%)
7 (63.6%)
4 (36.45)
Chemotherapy
Pemetrexed
Docetaxel
Paclitaxel
6 (26%)
2 (33.3%)
3 (50%)
1 (16.7%)
Tyrosine kinase inhibitor
Erlotinib
High
Standard
Gefitinib
High
Standard
Osimertinib
High
Standard
18 (78.2%)
3 (16.7%)
-
3 (33.3%)
6 (66.7%)
1 (16.7%)
5 (83.3%)
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Table 4. Responses in patients receiving intrathecal therapy.
Response Number of patients Intrathecal therapy
(Number of patients)
Complete response 1 Intrathecal methotrexate (1)
Partial response 2 Intrathecal therapy (2)
Triple intrathecal therapy (1)
Intrathecal methotrexate (1)
Stable disease 11 Intrathecal therapy (6)
Triple intrathecal therapy (2)
Intrathecal methotrexate (4)
No intrathecal therapy (5)
Progressive disease 17 Intrathecal therapy (2)
Triple intrathecal therapy (0)
Intrathecal methotrexate (2)
No intrathecal therapy (15)
Figure 2. OS graph. OS in days with its 95% condence interval is depicted. The median OS value is depicted with dashed lines.
Discussion
LMM is a devastating complication associated with solid malignancies with limited treatment options and poor OS. To the best of authors’
knowledge, this is one of the few studies that has retrospectively looked into four prospective single-centre trials on LMM in NSCLC. The
incidence of LMM in the current study is 0.03%. This is much lower than the incidence reported in multiple studies which ranges between
3% and 9% [4, 5]. The question is why was the incidence of leptomeningeal metastasis low in our study. The reason may be manifold. Firstly,
evaluation of the craniospinal axis was not carried out routinely. It was performed by physicians only when brain metastasis or LMM were
suspected. Hence, asymptomatic LMM was missed. This would have led to falsely low rates of LMM. Secondly, we used strict criteria for
diagnosis of LMM which were either cytological positivity or radiological evidence. The diagnosis of LMM based on biochemical or clinical
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parameters was avoided even though these recommendations are provided by the NCCN and the ESMO-EANO group [13, 14]. Thirdly,
access to second and third-generation TKIs and immunotherapy was restricted in our studies. Therefore, the 18-month OS rate in EGFR
mutation-positive patients in India is between 48.7 and 74.3% [9, 10] as opposed to the 18-month OS rate with third-generation TKIs of 85%
[15, 16]. Probably because of this, patients died due to extracranial progression (competing causes), thus limiting the probability of progres-
sion to LMM. Fourthly, our data were from a prospective cohort of four studies. As opposed to this, the incidence data in most of the studies
has come from retrospective studies with its likelihood of selection bias. Lastly, there might be a lower occurrence of LMM in Indian patients.
Time to develop LMM in the current study was 14.92 months. This is similar to the time to development seen in other studies. In a ret-
rospective analysis from Kuiper et al [17], time to development of leptomeningeal metastasis was 13·3 months. In our study, the trend of
occurrence of LMM was more in driver mutation-driven patients, which is in concurrence with previous studies. In a study by Li et al [6],
the incidence of LMM was found to be 9.4% in patients with EGFR mutations versus 0.7% in wild-type EGFR patients (p < 0·001). The
higher occurrence of LMM in patients with brain metastasis suggests that it is important to consider the occurrence of LMM in patients
with brain metastasis on follow-up [18].
The clinical features seen in our study had a predominance (nearly half) of higher mental function disturbance and seizures. This is in line
with the data published by Pan et al where adenocarcinoma-related LMM mainly leads to higher mental function symptoms [19]. Another
interesting aspect of the data was presentation of LMM with cerebrovascular accident-like features. This suggests that LMM probably should
be a provisional diagnosis when CVA-like symptoms are seen in NSCLC patients.
Nearly 40% of the patients in the current study did not receive cancer-directed treatment at LMM. This is in line with the data across the
world [20]. The outcomes of patients with poor performance status, fixed extensive neurological deficits or with extracranial progression are
dismal [21]. Hence, patients with these features are not treated routinely at our centre. The variable use of intrathecal therapies with sys-
temic therapies seen in the study is in concurrence with the limited efficacy of intrathecal therapies seen in the literature [1, 22]. There is no
evidence of improvement in OS or PFS with the use of intrathecal therapies [1, 22]. The median OS after development of LMM was around
2 months, which is consistent with that quoted in the literature [21–23]. As per our study, the use of intrathecal therapy had some form of
response among patients as compared to those who did not receive intrathecal therapy.
Our results are inferior to those reported in studies focussing on novel agents in driver mutated patients. The recently published BLOOM
study has shown promising results with the use of high-dose osimertinib (80 mg BID) in EGFR-mutated NSCLC with LMM [25]. In our study
also there was a trend towards higher OS in patients receiving osimertinib. However, the numbers were few (6 out of 31) to have adequate
power for a statistical analysis. Thus, our data along with the literature suggests that non-driver mutated patients have poor prognosis and
even driver mutated patients would have similar prognosis if exposure to third-generation novel agents is not possible. The outcomes of
EGFR-mutated NSCLC treated with first-generation tyrosine kinase inhibitors can be improved by administering either VEGF inhibitors [26]
or with the addition of chemotherapy [10] or alternatively by substituting it with novel third-generation agents [18]. Moreover, the results
imply that in patients with LMM, the use of agents like osimertinib is warranted.
The study limitations are that it was carried out among the Indian population and in a single centre. Access to third-generation therapies and
immunotherapy was limited. However, this is also a strength as it is a pointer towards the likely outcomes in LMICs where access to these
agents are limited. The strength of the study is the strict criteria used for LMM diagnosis and the radiology review for confirmation of the
presence of LMM.
Conclusion
The incidence of LMM is low in the Indian population. In our study, there was no single factor which impacted survival in patients who developed
LMM. This suggests that the overall prognosis is poor in patients with LMM where access to newer therapeutic modalities is limited.
Funding
No funding was received for this study.
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Conicts of interest
The authors declare that they have no conflicts of interest.
Authors’ contribuons
Vijay Patil and Kumar Prabhash – concept, design, execution, data collection, analysis, interpretation, first draft and final draft.
Vanita Noronha – execution, data collection, interpretation, first draft and final draft.
Dilip Harindran Vallathol – concept, data collection, interpretation, first draft and final draft.
Nandini Menon, Abhishek Mahajan, Amit Janu and Nilendu Purandare – execution, data collection and final draft
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