Article

In vitro digestibility, physicochemical and sensory properties of a gluten‐free biscuit from blends of cassava and African walnut flour

Wiley
Journal of Food Processing and Preservation
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Abstract

The study assessed the nutritional and sensory properties of a gluten‐free biscuit from blends of high‐quality cassava flour (HQCF) and walnut flour (WF). Design expert software was used to determine the compositing level with HQCF (minimum of 70 g and maximum of 80 g) and WF (minimum of 15 g and maximum of 30 g). Supplementation of HQCF with WF significantly influenced the water absorption capacity of the flours. Carbohydrate (58.04‐67.72%), diameter (2.98‐3.70 mm) and thickness (0.54‐ 0.79 mm) content of the biscuit decreased with increasing WF substitution, while protein (7.04 ‐ 10.50%), in vitro protein digestibility (16.99 ‐ 18.61 %) and fat (17.00 ‐ 22.33 %) of the biscuit samples increased with increasing levels of WF. Antinutrient content including oxalate (0.15 ‐ 0.25 mg/g), phytate (0.07 ‐ 0.25 mg/g), cyanide (0.03 ‐ 0.11 mg/g) and tannin (0.10 ‐ 0.36 mg/g) of the biscuits were within recommended safe limits.

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... Cassava not only provides daily calorific requirements of about 300 kcal/person/day but is also a source of income to more than 70% of value chain actors mainly farmers and wholesalers) (UBOS 2019). With an increasing demand for gluten-free high-quality cassava flour (HQCF) in industries ranging from pharmaceutical to paperboards, confectionary, and breweries (Nuwamanya et al. 2019;Oyeyinka et al. 2022), the adherence to quality standards, such as CODEX STAN 193 (FAO-WHO 2017), adopted by East African Community has pushed for production of quality cassava flour both for domestic and industrial use. ...
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Egyptian dieticians typically rely on foreign databases to find out oxalate content of food due to unavailability of local databases. The soil, fertilizers, climate and cultivars are often very different. Therefore, the purpose of this study is to establish a local database of oxalate content in Egyptian grown fruits and vegetables and selected daily common herbs. The current study analysed the total and the soluble oxalate in 37 Egyptian grown fruits, vegetables and 9 commonly used herbs. Two methods were used for screening the Egyptian foods for oxalate concentration; the first method was AOAC 1999 and the second was enzymatic method. Total oxalate varied greatly among the vegetables examined, ranging from 4 to 917 mg/100 g F.W. Total oxalate of analysed fruits ranged from 9 to 50 mg/100 g F. W. There is a strong correlation found between the two methods used. Vegetables were classified into 4 categories; low oxalate concentration containing less than 10 mg of oxalic acid /100 g F.W., such as cabbage, courgette, cucumbers, garlic, spring onions and turnip. Moderate oxalate concentration vegetables containing 10-25 mg/100 g F. W., such as aubergine, field bean, corn, peppers and watercress. High oxalate concentration vegetables containing 26-99 mg/100g F.W., such as f?l, green beans, celery, mallow, okra and sweet potatoes. Very high oxalate concentration containing 100-900 mg/100g F.W. such as Swiss chard, molokhia, purslane and vine leaves (fresh). Extensive amounts of total oxalate (201-4014 mg/100 g D.W.) were found in daily common herbs such as caraway seed, green cardamom, cinnamon, coriander seeds, cumin, curry powder, ginger and turmeric powder.
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Cassava (Manihot palmata) and soybean were processed into flours and used to substitute wheat flour as composite flour. The wheat flour (WF) was substituted by cassava flour (CF) at levels of 0, 10, 20, 30, 40, 50, 60, and 70%; while the resulting composite flours at levels above 40% were replaced with 10% soy flour (SBF) to increase their protein levels for biscuit production. This study evaluated the physicochemical and sensory qualities of biscuits baked from the various formulations of the flours. The proximate composition, hydrocyanic acid (HCN), spread ratio, biscuit weight and sensory properties of the biscuits were measured. Trained panelist evaluated the differences in organoleptic properties using a consumer sensory panel. Colour, crispiness, taste and flavour of biscuit reduced with increase in cassava flour but the diameter, spread ratio and shape of the biscuit improved. Increase in the levels of cassava flour resulted in decrease in the protein content progressively from 13.04% in 100%WF to 8.4% in biscuit with 40% cassava flour. However, addition of 10% soybean flour to composite flour above 40% CF resulted in progressive rise in the protein content of the biscuit from 8.41% in the 40% cassava flour biscuits to 11.39% in biscuits with 50%CF with subsequent reduction as the cassava flour increased to 70% level. The hydrogen cyanide content of the biscuits was increased with the increase in level of cassava flour in the formulations with maximum level of 0.20 mg/kg and in biscuit with 70% cassava flour. There was no significant difference in the overall acceptability between the biscuit from the control (100% wheat flour) and the composite flours up to 7040% cassava substitution level. Further addition of soy flour above 40%CF levels also improved the overall acceptability and there was no significant difference in the overall acceptability sensory qualities and other biscuit without soy flour enrichment up to 60% cassava flour. This indicates the feasibility of producing nutritious biscuits with desirable organoleptic qualities from cassava/wheat/soy composite flour up to 7060% cassava substitution level.
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original scientific paper Summary The biscuit making potentials of wheat and plantain (stages 1 and 3 ripeness) flour blends were evaluated. Composite flours were formulated using wheat (white) and plantain flour in ratio 90/10 1 , 80/20 1 , 50/50 1, 100/0, 90/10 3 , 80/20 3 and 50/50 3 at stages 1 and 3 respectively with the superscripts representing the stage of ripeness. The functional properties of the flours, proximate composition, mineral composition and sensory attributes of the biscuit were evaluated. The loose bulk density (LBD) of flours ranged from 0.46-0.53 g/mL, the packed bulk density (PBD) ranged from 0.64-0.76 g/mL. The water absorption capacity (WAC) ranged from 1.19-2.02 g water/g flour and oil adsorption capacity (OAC) ranged from 0.50-1.00 g oil/g flour. The LBD and PBD values of the composite blends were higher than the control (100 % white wheat flour-sample D). The proximate composition ranged from 11.00 %-9.96 % for moisture, 1.21 %-1.27 % for ash, 2.98 %-4.30 % for fat, 11.82 %-12.64 % for protein, 0.97 %-1.52 % for fibre and 69.56 %-71.21 % for carbohydrate. The protein and carbohydrate value of biscuit produced from composite flour 80/20 3 (sample F) was significantly (P ≤ 0.05) lower than other biscuits. Composite flour 50/50 3 had the highest protein content (12.64 %). The observed mineral composition varied among the samples. The content of calcium, iron and phosphorus ranged from 5.89-6.14, 0.48-0.78 and 21.97-23.46 mg/100g respectively. The calcium and iron contents of biscuit produced from flour blends F was significantly (P ≤ 0.05) higher than other biscuits, while the phosphorus content of biscuit produced from composite flour 90/10 1 (sample C) was higher than other biscuit samples. Sensory evaluation of the formulated biscuit samples showed similar sensory attributes with the control.
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Cookies were produced from germinated pigeon pea, fermented sorghum, and cocoyam flour (CF) blends to determine their potentials in cookie manufacture. Ten flour formulations were produced and they were evaluated for their proximate and functional properties. Protein content ranged from 4.85% to 19.89% with 100% CF (100CF) having the least value, while 100% germinated pigeon pea flour (100GPF) had the highest value. Increase in levels of GPF to the flour blends resulted in increase in protein content of the blends. Cookies made with 100% fermented sorghum flour (100FSF) had the highest ash content of 2.73%, while cookies made with 100GPF had the least ash content. Energy values of the cookies ranged between 369.37 and 376.56 kcal/100 g, with cookie formulation 50%CF:50%FSF having the least value and cookies made with 16.7%CF:16.7%FSF:66.6GPF having the highest value. The control (cookies made with wheat) had the highest spread ratio of 24.13, while cookies made with 100FSF had the least spread ratio of 14.97. Cookies made with 100CF were the least fragile. Sensory ratings revealed that cookies containing up to 50% CF and above, compared favorably with those made with wheat flour.
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Cookies were produced from pigeon pea (Cajanus cajan), sorghum (Sorghum bicolor) and cocoyam (Xanthosoma sagittifolium) flour blends. Ten different cookie formulations were produced. The cookies were evaluated for proximate composition, antinutritional factors and protein quality. The protein content ranged from 6.40% for cookies from 100% cocoyam flour (CF) to 12.97% for cookies from 100% pigeon pea flour (PF). Only four out of the ten cookie formulations had the FAO/WHO minimum recommended protein content of 10%. Low levels of antinutritional factors such as tannins, oxalate, saponins, trypsin inhibitors, hydrogen cyanide and phytates were observed. Diets made from cookies with minimum protein content of 10% were similar to the casein diet in maintenance weight, food intake, digested nitrogen, nitrogen balance, biological value and net protein utilization when fed to rats. It is concluded that cookies from the flour blends showed promise in helping to combat protein malnutrition in those countries where protein malnutrition poses a threat.
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Sorghum seeds of cultivar Wad Ahemed (phytate: 2.7 mg/g, tannin: 0.96% and 2 h pepsin digestion: 18%) were germinated for three days to obtain 1-, 2- and 3- days old sorghum malts. Sorghum malt was added in concentrations of 1, 2.5, 5, 7.5 or 10% to sorghum flour. The mixtures were incubated with shaking for 0, 30, 60, 90 or 120 min. Phytic acid and in vitro protein digestibility were assayed for all treatments. The results revealed that phytate content was significantly reduced. The 10% 3-day-old malt after 120 min incubation, reduced the phytate content by 83%. The in vitro protein digestibility (IVPD) was significantly improved as a result of malt pretreatment. The rate of reduction of phytate content and the rate of increment in IVPD increased with time of incubation, age and concentration of the malt.
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Objective There is no enough information on the classification of oxalate content in several foods, particularly in ethnic foods, to recommend their consumption in subjects with urolithiasis (UL). The objective of the present study was to generate reliable information on the oxalate content and antioxidant activity in different foods and classify them by very low, low, medium, high and very high oxalate content and antioxidant activity. Methods The oxalate content of 109 foods including ethnic foods was assessed by an enzymatic assay, and the antioxidant activity was measured by the oxygen radical absorbance capacity to determine the oxalate/antioxidant activity ratio. Oxalate consumption was then evaluated in 400 subjects with overweight and obesity using 24-h dietary recalls. Results The main foods with high oxalate content were raw spinach, huanzontle, purslane, chard, almond, and toasted and sweetened roasted amaranth. The highest antioxidant activity was found in strawberries, all types of chocolates, roselle, morita peppers, and pinolillo. Subjects with overweight or obesity exceed the dietary oxalate daily intake recommendation. Conclusions The classification of foods by their oxalate content and antioxidant activity will be very useful to generate nutritional recommendation in different diseases, mainly UL.
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The effect of wet cooking on the nutritional and functional properties of two Bambara groundnut landraces and a reference dry bean was studied. Wet cooking increased the amino acid concentration of the Bambara landraces for most of the amino acids tested, but the methionine content of the two Bambara landraces and the reference bean decreased by approximately 90, 90 and 20%, respectively. Carbohydrate and fiber contents of all the samples increased after cooking. Cooking led to a significant increase in the total phenolic content of the grains and decreases in phytic acid and tannin contents, which seems to have resulted in an increase in the in vitro starch and protein digestibility of all the samples. Thermal processing had varying effects on the conformation of proteins as observed in the FTIR spectra of each sample. However, nutrients were well retained and antinutrients reduced significantly.
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The cassava flours and starches have elicited great use in the food and non-food industry. The diversity in cassava genotypes accounts for differences in end-product properties, and would require characterization of cassava varieties for suitability of culinary and processing. This review showed that screening criteria of cassava cultivars end-user properties include proximate contents, amylose content, structural, swelling, gelatinization and pasting characteristics, including freeze–thaw stability properties of cassava-derived flours and starches. Literature shows that the physiochemical properties vary with genetic factors (i.e. genotype). In this review, the amylose content was found to be the main genetic trait for discriminating the cassava varieties for gelatinization and pasting processes including resistant starches. Moreover, cassava derived raw materials (flours and starches) were found to have various application in baking, edible film, syrup, glucose, alcohol, and soups production.
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The effect of processing methods on the nutritional, anti-nutritional and functional properties of African walnut was investigated. The proximate composition of African walnut (Tetracarpedium conophorum) on % wet basis showed protein (14.92), oil (45.84), fibre (1.14), ash (3.52) and carbohydrate (15.38). Evaluation of its anti-nutritional factors (mg/100g) revealed tannins (0.89), oxalate (1.28), phytic acid (3.105), trypsin inhibitors (1.84), saponin (985.0) and alkaloid (40.91) as being inherent to the edible nut. These constituents were significantly (p>0.05) decreased by cooking while toasting resulted in their increased concentrations but to a level found not harmful to the human body. The functional properties; water absorption capacity (1.68m/g), oil absorption capacity (1.06ml/g) and foaming capacity (6.2%) were generally improved by processing. Refrigeration, storage at room temperature (27-32°C) and addition of 0.01% ascorbic acid to walnut flour effectively extended the shelf life of the product.
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Nutritional quality of snacks prepared from cereals can be improved by the addition of crude lycopene (CL), tomato powder (TP) and saffron extracts (SE) which are known for their high photochemical content. The study was undertaken to prepare extruded snacks from whole wheat flour enriched with CL, TP and SE. Effect of extrusion on physicochemical, antioxidant and sensory properties of snacks was also investigated. Hardness of snacks containing CL and TP was higher than the control. Pasting properties of the formulations were significantly (p < 0.05) reduced upon addition of CL and TP. Total phenolic content (TPC) of the formulations with added TP increased significantly (p < 0.05). Antioxidant properties of snacks enhanced after enrichment. Extrusion significantly (p < 0.05) reduced the TPC, DPPH scavenging activity and pasting properties of snacks while, reducing power and inhibition of lipid peroxidation increased. Storage period lowered the hardness and antioxidant properties of snacks.
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With the utilization of enzymes including endoxylanase, glucose oxidase (GOX) and transglutaminase (TG), and emulsifiers comprising sodium stearoyl lactate (SSL) and soy lecithin, the microstructural, textural, and sensory properties of whole-wheat noodle (WWN) were modified. The development time and stability of whole-wheat dough (WWD) were enhanced by TG due to the formation of a more compact gluten network, and by SSL resulting from the enhanced gluten strength. Microstructure graphs by scanning electron microscopy (SEM) verified that TG and SSL promoted the connectivity of gluten network and the coverage of starch granules in WWN. TG increased the hardness and elasticity of cooked WWN, while two emulsifiers increased the noodle cohesiveness. Additionally, TG and SSL improved the sensory properties of noodle such as bite, springiness, and mouth-feel. The results suggest that TG and SSL are effective ingredients in enhancing the gluten strength of WWD and improving the qualities of WWN.
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The amino acids content of conophor nut (Tetracarpidum conophorum) was determined. The total essential amino acids (TEAA) was 274mg/g crude protein out of a total amino acids of 573mg/g. The most concentrated amino acid was glutamic acid (134mg/g). Leucine has the lowest essential amino acid score of 0.32 thereby making it the first limiting amino acid. Isoleucine had the highest level of score (0.80) followed by lysine with a score of 0.70. The leucine/isoleucine ratio was 0.71. Since most of the essential amino acids were high, it could serve as a food supplement.
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The production of high quality functional pasta from non-conventional raw materials represents a challenge. A partial substitution (15 g/100 g) of durum wheat semolina with long-chain inulin (HPX) and short-chain inulin (GR), Glucagel, psyllium and oat material (added individually and in combinations) was performed in order to increase the level of dietary fibre intake. The cooking, textural and colour characteristics of the pastas were evaluated and compared to control sample containing exclusively durum wheat semolina. Generally, material addition to the durum wheat pasta increased cooking losses, swelling index and water absorption, whilst reduced firmness and resistance to uniaxial extension of pastas. Raw spaghetti samples resulted significantly darker (L*) and more redness (a*) than control pasta. In the cooked pasta, all inulin enriched samples were brighter than semolina pasta. Pasta prepared with 15 g/100 g semolina of oat flour showed the best performance (except for the colour) compared to the other experimental pasta samples, but was significantly different to control durum wheat sample. Combinations of fibre rich additions were studied with the inclusion of inulin GR having a less deteriorating effect when added in combination with oat flour. This illustrates that some fibre rich sources may act better in combinations than separately.
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Anti-nutritional factors (ANF) are compounds which reduce the nutrient utilization and/or food intake of plants or plant products used as human foods or animal feeds and they play a vital role in determining the use of plants for humans and animals. Apart from cyanogenic glycosides, food poisoning arising from anti-nutritional factors, otherwise known as plants' secondary metabolites has not been properly addressed in most parts of the developing world. People have died out of ignorance, poverty and inadequate nutrition information and education, especially within the African societies. There are reports from time to time of deaths after consumption of some type of beans despite cooking. Also, cases of renal and liver diseases are increasing and this calls for a need to properly address the issue of thorough and adequate processing of foods/feeds before consumption. The aim of this review is to emphasize on the adequate processing of foods/feeds and to educate the people on the dangers of consuming improperly processed foods especially legumes which are reported to contain very high concentrations of anti-nutritional factors.
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Cassava South China genotype cultivars were released by Chinese Academy of Tropical Agricultural Sciences and their physicochemical traits had been described. However, the physicochemical traits of these cultivars affected by environmental factors were poorly understood. This study aimed to investigate the effects of interrelationship between genotypes and environments (G and E) on physicochemical traits of cassava roots and starches. Fourteen quantitative characteristics were assessed in seven genotypes grown in eight locations in China. The influences due to G and E upon the physicochemical traits of cassava roots and starches were evaluated using the general linear models procedure. The results indicated that the examined traits could be significantly affected by the factors of G and E, and the different contribution of G and E to total variation was evidenced. A most important economic trait dry matter content, ranging from 18.3 to 31.9% during seven genotypes, was negatively correlated to rainfall in cassava growing environments, but insignificantly correlated to temperature. Average starch granule sizes ranged between 9.5 and 12.7 µm. Granules were mainly truncated in shape and similar across all cultivars, and were insignificantly affected by rainfall and temperature. Significant correlations were observed among starch properties including paste clarity and viscosity, which was positively correlated to granule surface area. Principal component analysis demonstrated that SC8 is the best genotype suited for industrial applications and all cassava genotypes responded differently in various environmental climate conditions for the examined traits.
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Cassava is a valuable source of food for developing countries, but it contains highly toxic cyanogen compounds and antinutrients. Cyanogens are found in 3 forms in cassava: cyanogenic glucoside (95% linamarin and 5% lotaustratin), cyanohydrins, and free cyanide. Different processing techniques exist to remove cyanogens and their effectiveness depends on the processing steps and the sequence utilized, and it often is time-dependent. Pounding or crushing is the most effective for cyanogenic glucoside removal because it ruptures cell compartments, thus allowing direct contact between linamarin and the enzyme linamarase that catalyzes the hydrolytic breakdown. Crushing and sun-drying cassava roots made into flour removes 96% to 99% of total cyanogens, whereas soaking and sun-drying into lafun or fufu, or soaking and fermenting and roasting into gari or farina, removes about 98% of cyanogens. For cassava leaves, which have 10 times more cyanogens than roots, pounding and boiling in water is an efficient process to remove about 99% of cyanogens. Other strategies to reduce toxicity include development of low-cyanogen cassava varieties and cassava transgenic lines with accelerated cyanogenesis during processing. Although phytate and polyphenols have antioxidant properties, they interfere with digestion and uptake of nutrients. Fermentation and oven-drying are efficient processing methods to remove phytate (85.6%) and polyphenols (52%), respectively, from cassava roots. Sun-drying the leaves, with or without prior steaming or shredding, removes about 60% phytate. Cassava is a nutritionally strategic famine crop for developing countries and, therefore, reducing its toxicity and improving its nutritional value is crucial.
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Cookies (soft type biscuits) were produced from blends of wheat flour containing graded levels (0–25%) of protein concentrates prepared from ungerminated and germinated fluted pumpkin (Telfairia occidentalis Hook) seeds and evaluated for nutritional, baking and sensory properties. Protein quality was investigated using weanling albino rats fed diets that were formulated to supply 10% protein using cookie samples, with casein as a control. Cookies produced from blends containing protein concentrates from germinated seeds had higher contents of crude protein and lower levels of polyphenol and phytic acid, compared with cookies supplemented with concentrates from ungerminated seeds. The use of up to 15% concentrate from ungerminated seeds in the blends produced cookies with spread ratio, hardness, colour and flavour similar to the 100% wheat flour (control) cookies. Cookies supplemented with concentrates from germinated seeds at 15–25% levels were nutritionally comparable to diets based on casein, but at the expense of acceptability. Copyright © 2004 Society of Chemical Industry