Technical ReportPDF Available

DAIMON Ecotox Toolbox in a nutshell Short practical guidance to explain and apply the Ecotox Toolbox (in simple version)

January 2021

DOI:10.13140/RG.2.2.28450.50886

Affiliation: Thünen Institute

Authors:

Thünen Institute

For a certain marine area you want to know if there is dumped munition present, and if substances are leaking and may affect biota. You want to use the Ecotox Toolbox. This short manual explains how to to so.

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Technical Report

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DAIMON ECOTOX TOOLBOX Toolbox for the assessment of marine munitions' impact on biota

January 2021

With this modular Toolbox we provide a strategy and methodological tools to answer the question whether the marine ecosystem might be affected by dumped munitions and potentially hazardous chemical munitions components. The Toolbox is designed for use in monitoring and assessment as well as supporting decision making.

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Presentation

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DAIMON -EcoTox Toolbox (Kurz) Entscheidungshilfe zur Beurteilung der Auswirkungen versenkter Munitio...

November 2020

Entscheidungshilfe zur Beurteilung der Auswirkungen versenkter Munition in der Ostsee

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Conference Paper

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With PAH metabolites all around Europe

May 2013

Ulrike Kammann

Polycyclic aromatic hydrocarbons (PAH) are important environmental contaminants which may lead to reproductive effects or formation of liver tumours and associated lesions in fish. Therefore PAH and their biological effects are part of international monitoring programmes and recommendations (e.g. COMBINE, JAMP/CEMP and CORESET). In the light of the European Marine Strategy Framework Directive (EU ... [Show full abstract] MSRL) monitoring data and assessment criteria (AC) are brought together for evaluating the environmental status in marine and coastal regions. Usually this is done on a regional scale. ACs for biota are species specific and may differ between the regions. PAHs are rapidly metabolised and excreted via bile in vertebrates such as fish. To assess the PAH exposure of fish, concentrations of the main metabolite 1-hydroxypyrene, was determined in bile of different marine fish species by HPLC with fluorescence detection. The absence of time trends suggested that results of different sampling campaigns and years could be combined. The map of the PAH metabolite 1-hydroxypyrene in fish from different European regions gave a comprehensive picture, even with ACs applied. The results show that PAH metabolite can be used to create a spatial overview on PAH contamination in European marine fish. Concentrations of PAH metabolites differed primarily according to sampling region and secondarily to species. The results are compared to recently developed assessment criteria.

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Poster

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Assessing the Ecotoxicological Potential of Carbamazepine – Evaluation of a Behavioural Parameter in...

May 2017

The pollution of the aquatic environment is currently characterised by a great number of contaminants, especially by mixtures of micro-pollutants. The ecotoxicological implications of this burden is not yet fully assessable. The anticonvulsant carbamazepine was analysed using a newly developed test. The change of frequency of spontaneous tail movements occurring during embryogenesis of Zebrafish ... [Show full abstract] (Danio rerio) was utilised as a sensitive endpoint to assess the neurotoxic potentials of substances. The method was validated using a positive control. After fertilisation, Zebrafish embryos were transferred to 24-microwell-plates and exposed to carbamazepine. The embryos were incubated at 28,5 ± 0,5°C. Effects on behaviour were examined after 24 hours of exposure by videotaping (30 seconds) each well and quantifying spontaneous locomotion in comparison to a negative control. Carbamazepine exposure showed decreasing, concentration-dependent effects on embryonic locomotion. The determined data was compared to acute and chronic ecotoxicity data found in literature. The results of the behavioural based assay displayed a considerable increase of sensitivity compared to results from the Fish Embryo Acute Toxicity Test and the chronic Fish Early-Life Stage Toxicity Test. This work illustrates the sensitivity and specificity of the established endpoint within the developed test method which makes it possible to reliably assess adverse, neurotoxic effects of compounds on the model organism Danio rerio. This can be a substantial contribution to an effect-based risk assessment of contaminants in aquatic ecosystems.

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DAIMON Ecotox Toolbox in a nutshell Short practical guidance to explain and apply the Ecotox Toolbox (in simple version)

Abstract

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DAIMON ECOTOX TOOLBOX Toolbox for the assessment of marine munitions' impact on biota

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