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The Eect of Royal Jelly on Depression and Anxiety in an Animal Model of Alzheimer’s Disease
Maryam Azimpour1, Mohammad Fathi*2, Omid Dezfoulian3
1Department of Physical Education and Sport Sciences, Faculty of Literature, Lores
tan University, Khorramabad, Iran
2Department of Physical Education, Faculty of Humanities, Lores
tan University, Khorramabad, Iran
3Department of Pathobiology, School of Veterinary Medicine, Lores
tan University, Khorramabad, Iran
*Corresponding Author: Mohammad Fathi
Email: Fathi.m@lu.ac.ir
Keywords:
1. Depression
2. Anxiety
3. Swimming
Introduction: Royal Jelly (RJ) may exert positive eects on the function of the central nervous
sys
tem. The aim of the present s
tudy was to inves
tigate the eect of RJ on depression and
anxiety in trimethyltin (TMT)-induced Alzheimer’s disease (AD) model in rats. Materials
and Methods: 32 rats were randomly divided into four groups; healthy (HC), AD, sham (SH),
and RJ-treated. Forced Swimming Tes
t (FST) for depression and Elevated Plus-Maze (EPM)
for anxiety assessment were performed. Gene expression in the samples was measured using
Real-Time PCR. Results: The dependent variable of immobility signicantly increased in
the AD group compared to the HC and RJ groups. Furthermore, the SH group has shown
greater immobility than the HC group. The antioxidant indexes of superoxide dismutase and
glutathione peroxidase were signicantly greater in the RJ group compared to the SH and
AD groups. These indexes were also higher in the HC group than in the SH and AD groups.
The RJ and HC groups exhibited a signicant reduction in anxiety behaviours compared to
the other groups. Conclusion: RJ supplementation has the ability to modulate the mood in
the TMT model of AD in rats and may exert benecial eects in the treatment of AD.D
ABSTRACT
Article Info:
Received: 28 June 2020 Revised: 29 Nov 2020 Accepted: 14 Dec 2020
[ DOI: 10.52547/shefa.9.2.79 ] [ Downloaded from shefayekhatam.ir on 2022-11-28 ]
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Fathi.m@lu.ac.ir
ه ديكــــــــچ
SHADHC
FSTRJ
EMP
SHRJHCAD
HC
ADSHRJ
ADSHHC
HCRJ
RJ
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AD
AD
AD
AD
TMT
TMT
CNS
–
TMT
TMT
1 Dementia
2 Alzheimer›s disease; AD
3 Organotin
4 Trimethyltin chloride; TMT
5 PVC
6 Central nervous sys
tem; CNS
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TMT
TMT
TMT
HC
TMT
ADC
SHTMT
RJ
RJTMT
Luacra
RJ
mgkg
FST
TMT
RJRJ
RJ
RJ
RJ
TMT
–
7 Royal Jelly; RJ
8 Sprag dowley
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EMP
EMP
OAE
OAT
GPXSOD
–
RNA
RBBuer
mercaptoethanol–β
CollectionTube FilterColumn
RNAElutionTube
cDNA
K
RealtimePCR
β2mAlleleIDv
RNAcDNADNA
PCR
cDNA
DNAPCRRNA
PCR
RealQ2x Mas
ter mix Green DyePCR
AMPLQON
ΔΔCt
9 Elevated Plus Maze
10 Open Arm Entries
11 Open Arm Time
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MANOVA
SODGPX
OATOAE
B2m
12 Multivariate analysis of variances
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P>
SOD
F GPX OAE OAT
P>
SOD
13 Shapiro-Wilk Tes
t
14 Levene’s Tes
t
15 Pillai’s Trace
16 Wilks’ Lambda
17 Hotelling’s Trace
18 Roy’s Larges
t Root
19 Scheee pos
t hoc tes
t
OATSODGPX
OAE
P>
SOD
GPX
OAE
OAT
TMT
RJ
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TMT
CNS
TMTTMT
EMP
TMT
TMT
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OAEOAT
TMT
OATOAE
TMT
RJ
RJ
TMT
GPXSOD
SODGPX
RJ
RJ
RJ
RJ
RJ
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RJ
SOD
RJ
ER
RJ
TMT
TMT
TMT
RJ
RJ
RJ
AMPN1oxideHDA
HDEA
HDEA
BDNF
RJ
tyr c
HDA
AMPRJ
adenosinemonophosphateAMPN1oxide
RJ
RJ
RJ
RJ
RJ
RJ
20 Brain-Derived Neurotrophic Factor
21 Tigo
22 Yangming
23 Es
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