➢ Anti-biofilm activity of quercetin and umbelliferone against some multi-drug resistant bacteria (Poster Presentation)

Abstract

Microorganisms produce polysaccharide-based structures, which can be used to adhere to the surfaces to protect themselves. Due to the protective nature of these structures, microorganisms can produce immediate responses to the adverse effects in their environment. Therefore, microorganisms that protect themselves cannot be easily removed from the surface they attached and this can lead to resistance. As a result of this, problems are experienced mostly in dental treatments, medicine, the food industry, water systems, drug resistance, and sterilization processes. Various studies continue to be carried out to eliminate the formed biofilm or to prevent the formation of biofilm. This study aims to observe the in vitro anti-biofilm activity of quercetin and umbelliferone against some MDR strains. The bacteria used in the study are standard Esherichia coli ATCC 25922, three clinical isolate MDR E. coli strains, Acinetobacter baumannii (MDR), Klebsiella pneumoniae (MDR), MRSA, and Shigella flexneri (MDR). According to the results of the experiment, dose-dependent biofilm activation and inhibition effects were observed. Further studies are required to put forward the mechanism of action of quercetin and umbelliferone on bacterial biofilm formation.

Full text

4th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2021) November 24-26, 2021.
www.EurasianBioChem.org
1136
➢ POSTER PRESENTATION
Anti-biofilm activity of quercetin and umbelliferone against some multi-drug resistant bacteria
Eda Altınöz1* ( https://orcid.org/0000-0003-1277-2019), Ergin Murat Altuner2 (https://orcid.org/0000-
0001-5351-8071)
*1Kastamonu University, Faculty of Science and Arts, Department of Biology, Kastamonu, Turkey.
2Kastamonu University, Faculty of Science and Arts, Department of Biology, Kastamonu, Turkey.
* Corresponding author e-mail: altinozedaa@gmail.com
Abstract
Microorganisms produce polysaccharide-based structures, which can be used to adhere to the surfaces
to protect themselves. Due to the protective nature of these structures, microorganisms can produce
immediate responses to the adverse effects in their environment. Therefore, microorganisms that protect
themselves cannot be easily removed from the surface they attached and this can lead to resistance. As
a result of this, problems are experienced mostly in dental treatments, medicine, the food industry, water
systems, drug resistance, and sterilization processes. Various studies continue to be carried out to
eliminate the formed biofilm or to prevent the formation of biofilm. This study aims to observe the in
vitro anti-biofilm activity of quercetin and umbelliferone against some MDR strains. The bacteria used
in the study are standard Esherichia coli ATCC 25922, three clinical isolate MDR E. coli strains,
Acinetobacter baumannii (MDR), Klebsiella pneumoniae (MDR), MRSA, and Shigella
flexneri (MDR). According to the results of the experiment, dose-dependent biofilm activation and
inhibition effects were observed. Further studies are required to put forward the mechanism of action of
quercetin and umbelliferone on bacterial biofilm formation.
Keywords: Biofilm, anti-biofilm, inhibition, MDR, quercetin, umbelliferone