Introduction: The present work describes a calorimetry-founded characterization and design of a potential surfactant based colloidal drug delivery system. However, numerous research groups have published their outcomes according to the thermodynamic characterization of mixed micelle formation but only a few articles provide deeper information on the temperature and composition dependence micellization in mixed surfactant systems combining the advantages of both aspects. For self-assembled surfactant systems, the most important parameter is the critical micelle concentration (cmc) value, which quantifies the tendency to associate and gives the value of Gibbs energy of micellization. Several techniques are known for determining cmc, but the isothermal titration calorimetry (ITC) deserves special attention in comparison to other procedures. The calorimetry gives an absolute straightforward thermodynamic characterization in a single experiment, includes cmc and binding enthalpy and this is it does not require other label material such as fluorophore, chromophore etc. as heat is a universal signal.