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Scientific evidence of mRNA and vectorial vaccines genotoxicity inducing tumors and psycho-neuro-behavioral disorders

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  • University Ambrosiana
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Abstract

Micro-RNAs (miR) are non-coding RNA filaments that control mRNA transcription. Micro-RNAs have been studied in cancer pathogenesis, metastasization, cancer therapy, the structuring of the central nervous system, diabetes, and heart disease. Mir-134-138 regulate the development of dendritic spines needed for synapses. Their silencing can lead to autistic spectrum disorders and mental retardation and damage to brains in evolution such as childhood and adolescence, producing learning problems and mood problems, and in adults for alterations of receptors for neurotransmission. It has been shown that N1-methyl pseudouridine binds to miR and induces silencing processes, increasing cell methylome at the origin of cancer. The production of mRNA vaccines replaces Uridine with N1-methyl-pseudouridine to escape innate immunity and implement rapid translation. N1-methyl-pseudouridine binding with mi-RNA alters the epigenetic transcription of oncosuppressor that, with the increase in cell methylation, could result in the induction of tumors and relapses, natural immunity inhibition, and neuro-behavioral disorders transmissible to progeny. Vectorial vaccines hybridize the host DNA with adenoviruses and induce tumors at the experimental level. Clinical reports and long-term epidemiological investigations are necessary to verify the impact of mRNA vaccines on health.
Scientific Reports of the Milan Shool of Medicine-Scuola Medica di Milano- November 26-2021
©Giuseppe R.Brera 2021
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Summary
Scientific evidence of anti COVID-19 mRNA and vectorial vaccines
genotoxicity inducing tumors and psycho-neuro-behavioral disorders.
Giuseppe R.Brera
1
Summary
. Micro-RNAs (miR) are non-coding RNA filaments that control mRNA
transcription. Micro-RNAs have been studied in cancer pathogenesis, metastasization,
cancer therapy, the structuring of the central nervous system, diabetes, and heart disease.
Mir-134-138 regulate the development of dendritic spines needed for synapses. Their
silencing can lead to autistic spectrum disorders and mental retardation and damage to
brains in evolution such as childhood and adolescence, producing learning problems and
mood problems, and in adults for alterations of receptors for neurotransmission. It has
been shown that N1-methyl pseudouridine binds to miR and induces silencing
processes, increasing cell methylome at the origin of cancer. The production of mRNA
vaccines replaces Uridine with N1-methyl-pseudouridine to escape innate immunity and
implement rapid translation. N1-methyl-pseudouridine binding with mi-RNA alters the
epigenetic transcription of oncosuppressor that, with the increase in cell methylation,
could result in the induction of tumors and relapses, natural immunity inhibition, and
neuro-behavioral disorders transmissible to progeny. Vectorial vaccines hybridize the
host DNA with adenoviruses and induce tumors at the experimental level. Clinical
reports and long-term epidemiological investigations are necessary to verify the impact
of mRNA vaccines on health.
The SARS-COV 2 pandemic developed in China most likely for a probable
laboratory induction linked to the attempt to create a hybrid virus "SARS-COV-HIV"
Scientific Reports of the Milan Shool of Medicine-Scuola Medica di Milano- November 26-2021
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for a vaccine or other purposes; the pandemic beginning determined a race to the
vaccine by "Big-pharma in opposition to the SARS-COV 1( 2002) and MERS
pandemics. (2009)" disappeared without mass vaccinations. Since 2002 until 2019, BIG-
Pharma omitted to invest in anti-SARS-COV vaccines aware that the speed of mutations
of SARS-COV like HIV would have made research unuseful. World countries public
health assessors, instead of determining a secondary prevention strategy to protect o
people with comorbidities at risk of lethality (92%) based on a careful study of the
literature on SARS-COV 1 for preventive purposes, wholly omitted the person-centered
indeterministic approach to medicine inspired to Person-Centered Medicine, the medical
science paradigm revolution
2
3
4
5
6
7
The omissions of the WHO and national
governments, based on an illiterate epistemological error leading to an approach to
pandemics with a mechanistic, linear model: "virus-infection- disease-death risk and not virus-
allostasis-natural immunity-vulnerability risk-disease risk- death risk" oriented only to a
mechanistic adaptive immunity induced by experimental genetic vaccines not tested for
long-term adverse effects with insufficient and criticized trials.
8
9
This illiteracy-based
error in promoting global health has resulted in a preventive strategy failure affecting
human rights and the economy, leading to 5 million deaths and in Italy to about 150,000
(at the date). In Italy, legislation induced vaccination with blackmail, such as the loss of
work which showed only for older than 39 a preventive efficacy in intensive care
admissions rate and lethality.
10
Epidemiological elaboration of data from the Italian "EPICENTRO Istituto Superiore
di Sanita'" ( October 9-November 10) shows in oldest people > 80 after two doses of
vaccines < 6 months an increase of lethality risk compared with unvaccinated (OR
1.59-IC q.1.2356 to 2.0587 P=0.0003 and a non-significant increase of lethality in the
vaccinated range 12-39: OR 1.2-CI 0.1691-8-8229
Table 1
In oldest people > 80, there is a higher mortality rate (OR = 1.5949, p < .001) for the
full vaccinated (two doses) within 6 months compared to unvaccinated and a tendency in
12-39.
Odds ratio
1.5949
95 % CI:
1.2356 to 2.0587
z statistic
3.584
Significance level
P = 0.0003
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There is a tendency to increase risk of lethality in the vaccinated compared to
unvaccinated in the range 60-79 close to 0,5 significance: OR 1.23 ( CI 0.5686 to 2.6962-
P =0,5904) present in 60-79 people.
In > 80, the lethality risk of vaccinated people is less than the full cycle of vaccination.
(OR = 1.07 ,CI 0.5397 to 2.1330P = 0.5904. In other ages, first doses and full cycle of
vaccination appear to be a protective factor from lethality, < 6 months from the first
dose.
To date, the vaccines' failure to determine a durable immunity longer than 3-4 months
for vectorial vaccines and six months for mRNA vaccines induced public health
assessors to induce a third dose boosting without any consideration of adverse effects
and possible alternative preventive measures.
11
The Italian population has been inundated with statements by the central health
government and regional governments driven media inducing vaccination or by mediatic
virologists also based on false public statements. It occurred with a vaccination
campaign for children, adolescents, and young people, not at risk of COVID-19, that if
rarely infected, they are asymptomatic and with a ratio of cases/fatality to zero or
almost. Roberto Speranza, the Italian Health Minister, declared "The full agreement of
all scientists" about the need for vaccination in all ages." Franco Locatelli, the director of
the Anti SARS-COV Italian Technical, stated on August 20 the inexistence of adverse
effects for adolescents to induce parents' authorization after the news of healthy
adolescents' deaths after vaccination from Italy and USA and signalization of adverse
effects by USA CDC, also in contrast with health policy of countries like the UK.
Lethality from COVID-19 concerns 92,8% of people with comorbidities
12
characterized by atherosclerosis which leads to an auto-immune phenotype and immune
anergy after the 7th day of the disease, the actual cause of lethality
13
confirming the
theories of the relativity of the infection to cholesterol concentration in lipid rafts and
the caveolar lipid rafts number and the probability of severe clinical syndrome relative to
the LDL/HDL ratio and phospholipase concentration in the cell membrane, one factor
altering the immune signal transduction.
14
Recently, Maurizio Federico, with a significant review
15
in a very profound and
straightforward way, highlighted that the mRNA vaccines have hard limits in
immunogenicity. Vaccines have limited usefulness in time and are restricted to RBD of
viral S-proteins of the original viral strain, losing effectiveness with variants because of
the "Original antigenic sin. Vaccines do not stop contagions because they do not
produce neutralizing antibodies (IGA) in mucous membranes of the pharynx and upper
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respiratory ways, so laws that lead to the obligation to vaccination certificates appear
without any scientific reason. They do not induce resident memory B-cells in lungs, not
preventing the first cause of lethality, but only IGG in the bloodstream. Moreover, they
select variants whose viral allostasis completely escape any previously vaccine-induced
immunity. Moreover, restrictions hamper the asymptomatics-and healed-induced herd
immunity, while there is the possibility to treat the infection early with efficacy,
16
identifying people at risk with the probability theory of the COVID-19 severity.
17
In the light of the only IGG stimulation and the lack of activation of resident
memory B-cells in the lung, the antiviral effectiveness of mRNA vaccines is a
conundrum.
Viruses lead to asymptomatic infections, depending on innate and adaptive
immunity, as happens for most people every week with different species of virus and
that for SARS_COV 2 is due to the immunization from other non-dangerous
coronaviruses, such as corona-adenoviruses which target at least 50% of people who
reach a partial immunity also to SARS-COV 2, because of common antigens. On the
other hand, infections depend on the protective factors that stimulate natural immunity,
eliminating the virus before it reaches the epithelial and endothelial cells. Infection is only
possible on the condition of degeneration of the cell membranes due to cholesterol and LDL, inducing the
formation of lipid rafts whose caveolae are the obligatory gates to the infection.
18
The oldest people
with atherosclerosisbased comorbidities are more at risk of lethality because of immune
anergy. Conversely, infections are rare and almost asymptomatic, with a low infectivity
index in children and young people with healthy cell membranes. It has been computed
that corona-adenoviruses immunize at least 50% of people. Antibodies against these
neutralize vectorial vaccines adenoviruses vectors. Vaccinations with viral vectors are
dangerous for children and adolescents closer to infections for the intensity of immunity
reactions leading to the risk of disease from immunocomplexes and an increased
thrombophilia.
19
20
In Italy and adolescents died of thrombosis after the boosting dose
of AstraZeneca.
The vaccines authorized by the Italian AIFA are genetic because they use genetic
mechanisms to induce an artificial immunity against a particular "Spike-protein" defined
by a messenger RNA code of the Huwan strain that allows the activation of adaptive cell
immunity through activated lymphocytes T and B that with their proliferation assure a
minimal immunity over time, not inhibiting the contagion.
21
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The mRNA vaccines are constructed with a sequence of bases homologous to
RBD of the viral spike protein. The code is then transcribed by the RNA transfert,
which allows the viral protein synthesis in the cytoplasm and in the organelles
(endoplasmic reticulum and Golgi apparatus) to form the new virus. The mRNA vaccine
is conveyed by lipid nano-particles that favor its entry into the cell membrane. The
infected caveolae of the lipid rafts alter the transduction of immunity signals inhibiting
proliferation and activation of lymphocytes T and B, but this is undoubtedly related to
the alteration of the cell membrane and the flooding of macrophages with cholesterol
and PH. Immunosenescence and the previous immune-atherosclerosis phenotype inhibit
adaptive immunity and memory-t and b cells production.
22
The virus dissemination is
relative to a previous immunosuppressive phenotype and "inflammaging" induced by
atherosclerosis inducing a lethality risk for the oldest people with comorbidities
associated with atherosclerosis, like diabetes, obesity, hypertension, cardiovascular
diseases. It explains the higher rate of mortality of the immunosenescent oldest people.
The SARS-COV 2 Immunity hijacking appears mediated by MTTL3, which blocks the
RIG-1 receptors recognition.
23
The mRNA vaccines induce a high IFN gamma reaction and stimulate CD4 TH1
cells in local lymph nodes, but neither induce the antiviral CD8+ mediated immunity
24
because they do not stimulate synthesis nor interact with the lung B-cell memory-
resident cells, not activating these. The immunity induction of mRNA vaccines is a
"conundrum."
25
The heterologous "mRNA" inoculated with the vaccine "infects" all the immune,
epithelial, endothelial cells, neurons in every anatomical structure, from the brain to the
heart, the endocrine organs, and the toe.
The problem that viro-immunologists
26
had to face was to prevent the inoculated
heterologous m-RNA from being recognized by Toll-like receptors (TLR).
27
For this
purpose, in m-RNA, they replaced a base: Uridine with n1-methyl pseudouridine, which
escapes immune control of the inoculated host and increases translation speed. In 2019 J.
Lockhart, J Canfield J, Mong EF et al.
28
demonstrated that the replacement of the Uridine of the
Spike Protein mRNA with the n1methyl-pseudouridine that is necessary for the mRNA production
alters the silencing of micro-RNA switches leading to a decrease in the activity of these molecular
switches, called "the dark matter" of the cell (about 50% of RNA) and thus altering the processes of
silencing. What happens if the repressor of an oncogenesis inhibitor is not silenced?
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N1-methyl-pseudouridine binds to miR, altering their action to silencing miRNAs,
possibly harming the organism's life. Micro-RNAs are filaments of non-coding RNA
that, thanks to the "Argonaut" proteins after joining the "RISC (RNA-induced silencing
complex, RNA-induced silencing complex), interact internally with the target RNA,
preventing transcription by preventing the synthesis of the protein with the specific
mRNA silencing. After their discovery in 1993 by Victor Ambros, Rosalind Lee, and
Rhonda Feinbaum, the role of particular microRNAs was studied in pathogenesis and
cancer therapy, in the structuring of the central nervous system, in diabetes, in heart
disease. For example, if mi-RNA 205 is inhibited in the pathogenesis of breast cancer,
carcinogenicity and metastasisation are encouraged. The same occurs for mi-RNA 21 for
liver cancer. In 2009 G. Schratt, with a great contribution, illustrated some fundamental
actions of miR in neurons. Mir-134-138 regulate the development of dendritic spines
needed for synapses.
29
Their alteration can lead to autistic spectrum disorders and mental
retardation and in brains in development as in childhood and adolescence, to learning
and mood problems, as well as in adults to neuro-transmission receptor alterations such
as CAMKII and CREB. Regulation of innate immunity involves mi-RNA 155-146 -132
as illustrated by J. Raisch, A.Darfeuille-Michaud, HT. Nguyen in their elegant review of
2013. Mir-155 regulates the suppression of the cytokine signaller (SOCS)-1, which
negatively regulates the capacity of the "Antigen Presenting Cells " APC to present
antigen and activate lymphocytes.
30
31
Cells with the lack of mir-155-show a defective
presentation of antigen and therefore cannot activate T cells to promote the TH1-
induced inflammation
32
: this could be the epigenetic pathogenesis of anergia and
immunosenescence. Another study has shown that the elimination of mir-155
expression significantly increases the expression of the pro-inflammatory IL1. These
observations depict how mRNA vaccines could induce paradoxical inhibition of innate
immunity, increasing people's vulnerability to infection and cancer. In atherosclerotic M2
immune phenotype, present in comorbidities at risk of COVID-19 severity,
33
it could
induce anergy when there are other infections with SARS-COV 2 variants escaping
previous and waning adaptive immunity in short-time, exposing oldest
immunosenescent people to a clinical syndrome severity up to lethality. Moreover, the
induced methylation by mRNA vaccines, resulting in an MTTL3 cellular increase,
supports the viral hijacking of immunity by SARS-COV 2 variants, and reducing natural
immunity. It means that more mRNA vaccines are inoculated, more natural immunity is
reduced, making easier variants’ immunity hijacking.
Numerous studies document how dysregulation of mi- RNA is associated with
cancer development and metastasis processes, as documented by the splendid review of
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G. Sotiropoulou, G. Pamplakis, E.Lianidou Pampalakis, Lianidou, Z. Mourelatos.
Cancer pathogenesis is associated with several bio-molecular processes such as genomic
alterations, transcription of oncogenic factors, and inhibition of repressors transcription,
such as P53 and hypoxia.
Epigenetic changes are regulated by micro-RNAs which are the arbiters of cell health as
on/of molecular switches of mRNAs.
Viral mRNAs such as mRNA vaccines act by altering microRNAs. The mRNA vaccines
act like a virus at the epigenetic level.
Recently E Karimi, H. Azari Yari, M, Tahmasebi, et al. identified 39 mi-RNA derived by
Sars-COV 2 inducing a viral allostasis inhibiting the innate immunity, altering Vit. D and
the lung cells metabolism through the transcription alteration.
34
The silencing of miR-223 appears to be caused by an epitranscryptomic alteration of pre-
micro-RNA, which produces an oncogenic factor that binds to its site, producing its
"switching off." This is associated with leukemia pathogenesis. An epidemiological study
on the incidence of post-vaccine leukemia and other cancers is needed. The direct
induction of miR dysregulation produced by mRNA vaccines can have dramatic
consequences for millions of young people and children by inducing the pathogenesis of
tumors or relapses and diseases of the central nervous system. What will be the effect of
mRNA vaccines on the brain of the ruling class since their action determines alterations
of the miRNA that control neuronal nuclei biological substrate of the cognitive and
subcortical sphere. What will happen with the impairment of the activity of the cerebral
cortex and the subcortical nuclei?
There is a pandemic of illiterate criminality in people who want to induce the vaccination of
adolescents and children who are not at risk of COVID-19. If rarely infected, children are
asymptomatic
35
thanks to their solid innate immunity and rapidly reduce the viral load in the oral and
nasal mucous membranes. Children and adolescents and asymptomatic people could act as "living
vaccines" contributing to the" herd immunity" as often occurs with other viruses. It has been well
highlighted that asymptomatic people relatively contribute to the virus diffusion. Vaccination exposes
children and adolescents to epigenetic and genetic damages whose impact must be studied with
epidemiological investigations but is sure.
Two meta-analyses show the reduced infectivity of asymptomatic people (AIC). Transmission rates of
AIC ranged from 02.2% compared to 0.815.4% for symptomatic (SIC)
36
and in the household from
04.9% compared to 18.0% of SIC.
37
.
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The infectivity reduction of asymptomatic people is probably due to the presence of
neutralizing IGA in mucous membranes, absent in vaccinated people.
A study that monitored 455 contacts exposed to the asymptomatic COVID-19 virus
carrier showed that nobody was infected.
38
The reduced viral load transmission by the upper respiratory and its disappearance in a
shorter time can explain this evidence and reverses the common belief that
asymptomatic people induce the pandemic. Conversely, a reduced transmissible viral
load to healthy people could activate the people's innate immunity and the progressive
loss of virulence by activating the tissue-resident memory T cells that block the virus
diffusion in the organism.
The right strategy to accelerate herd immunity is a health education campaign to educate
to assume immunogenic molecules (e.g., beta-glucans in bakers yeast) and inhale vapors
of powerful common natural antiviral substances at the first signals of infection of upper
respiratory ways.
39
The SARS-COV 1( 2002-2003) and MERS (2009-2010) disappeared without vaccines.
What will happen in millions of adolescents and young people not at risk of
COVID-19 that the Italian health management and other countries led to vaccination
with a legalized blackmail, because of the alteration of the regulation of mir-223, whose
alteration is linked to the pathogenesis of leukemia? In the USA, the FDA recently
approved mRNA vaccines in children based on a small Pfizer trial, which used children
as experimental animals and that did not monitor the adverse effects at the epigenetic
level and their manifestation after a long time.
The other process induced by mRNA vaccines is the cell's methylation induced by n1-
methylpseudouridine. The n1-methylpseudouridine (ɸ) stabilizes the RNA. It is naturally
present more in the RNAt, with implicit natural finalism to favor the coding probably.
In synthesizing the mRNA vaccines, the Uridine has been replaced with ɸ to increase
the translation speed and evade the natural immunity. However, introducing ɸ in each
cell produces cellular stress that could be equivalent to "heat stress" that produces an
'increase of cell methylome by methylating all the bases through the synthesis induction
of METTL3 (methyltransferase like 3) with the finalism to ensure an allostasis for
survival. The METTL3 binds to microRNA, causing the down-regulation of some and
up-regulation of others. The action of miR like that of antisense RNA could produce the
silencing of the P15 gene, which encodes a dependent cycline kinase involved as a
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repressor of malignant leukemic degeneration. W Yu, D Gius, P. Onyango, et al. in
2008 saw an inverse relationship between P15 and leukemia, highlighting the risk of its
repression. Antisense RNA also interacts directly or indirectly with DNA-
methyltransferase leading to DNA methylation and its consequences in the repression of
gene transcription and the recruitment of "histone-modifying enzymes" by modifying
chromatin.
This evidence means that in opposition to an illiterate bio-medical culture, mRNA
vaccines also induce genetic alteration that, in addition to induction or suppression of
miR, lead to DNA methylation and modification of chromatin.
Moreover, the increase in cell methylome could be caused by the lack of repression of
the MTTL3 synthesis by silencing its mRNA induced by a specific micro-RNA. The
MTTL 3 increase induces the general methylation of nucleotidic bases with catastrophic
effects, a sort of earthquake in the organism's life, with dramatic effects on the
pathogenesis of tumors.
In the tissues of patients with "Non-small cell lung cancer (NSCLC) (small cell lung
cancer) "N6-methyladenosine (m6A) methyltransferase-like 3 (METTL3)" regulates
microRNA-1246 (mir-1246) which is a well-documented tumor's progression and
metastases. More METTL3 and mir-1246 were found in these tissues in inverse ratio to
PEG-3 ( Paternally expressed gene-3).
40
The alteration of micro-RNA is, therefore, very dangerous. This confirms the aversion of Luc
Montagnier, Nobel Prize, against the use of genetic vaccines before knowing in depth their effects with
long-term epidemiological investigations.
Several deaths and adverse effects on mRNA vaccines have already been reported
worldwide,
41
such as the high incidence of pericarditis and myocarditis in young people,
which in some countries like the UK, has blocked any other vaccination in adolescents
and young people, not at risk of COVID-19. In Italy, a teenager died a few hours after
the second vaccination by m-RNA, another from the vectorial Astra-Zeneca. In the
USA, the CDC reported the deaths of 14 teenagers. The actual extent of these events is
entirely unknown because public health, organizations such as AIFA, the Ministry of
Health, the Italian Higher Institute of Health, to our knowledge, have not organized
epidemiologic research to study the adverse effects of these vaccines over time.
Undoubtedly the fear of COVID-19, induced by a virus quickly and destroyable and
neutralizable with natural antiviral remedies also immunogenic and drugs already in use,
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that prevents contact with epithelial and endothelial cells is derived from the lack of
public health orientation towards “Person Centered Prevention”
43
, and to failure in
primary and secondary prevention. This omission is due to ignorance and the non-
adoption in public health of the multi-factorial, multi-dimensional paradigm of "Person-
centered medicine," the paradigm change of medical science, that could have saved only
in Italy 150.000 and in the world millions of people. As Luc Montagnier claims, we need
analysis over a long time to control the existence of adverse effects, even fatal at a short
time. The only answer to the pandemic beginning and permanence has been the
adoption of "genetic" vaccines, which have been inadequately tested and limited in time,
with the scientific evidence of their danger. The adverse effects of these vaccines and the
induction of variants endanger the health of millions of people and must be prevented
or blocked in time to avoid a global health disaster, primarily protecting children and
adolescents whose health is to date menaced by mRNA vaccines.
The mRNA vaccines' dangers, as highlighted above, are shared by the viral vector
vaccines. These induce a modification of human DNA because of the recombination of
the animal or human DNA vector adenovirus with the host's DNA. This hybridization could lead
to auto-immune reactions and, at the experimental level, to an impressive induction of tumors.
44
Scientific evidence of the anti SARS_COV 2 vaccines- induced genetic damages must be studied with
clinical and epidemiological investigations. However, before it is necessary a total change of public health
administration resulting in the withdrawal of the authorizations to mRNA vaccines distribution and the
constitution of a metabolic and immune shield for the population by adopting the "Antiviral allostasis,
and immuno-stimulation"
45
strategy launched in Italy by the National Health Committee and in the
world by the World Health Committee.
Unfortunately, the prudence towards vaccines that led to their rejection only by a
minority of the population and health care workers has a sound scientific basis. The
dramatic suspension of health care workers who refused vaccination by a surprising (in
the negative sense) physician's council and discrimination of workers paradoxically
supported by unions - but unions should not defend workers? -that do not want to
vaccinate is without any scientific basis.
What can also happen to the brain, mind, and behavior of the national leadership,
institutional public or business, teachers, and in any context with the genes of the
adenovirus of chimpanzees integrated into the genome of cortical neurons or sub-
cortical brain (Astra-Zeneca vaccine) that, if in age, communicate to the progeny? Will
the COVID-19 contribute to human evolution?
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Clinical reports and long-term epidemiological investigations are necessary to verify the
impact of mRNA vaccines on health.
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Book
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. In the first part of the treatise, "The virus entry" the author had demonstrated how the SARS-COV 2 virus enters cells through pathological cell membranes characterized by lipid degeneration, thus explaining the vulnerability of the elderly with comorbidities that have atherosclerosis and protection against infection in young people as their common denominator. Adolescents and children are not subjected to immunosuppression due to a lack of alteration in the transduction of immune signals and the preserved validity of innate humoral and cellular immunity, verified by epidemiological research. This led Professor Brera to theorize an equation of relativity of the infection and probability theory of its severity, opening up a new perspective of prevention, capable of identifying subjects at risk of severity of COVID-19. In this second part of the treatise, the author deepened the new biochemical-immunological-virological perspective of viral allostasis, he introduced, the relationship between atherosclerotic pathology, immunosuppression, and immune anergia in people at risk, leading to lethality. The treatise completely falsifies the policy of even multiple vaccinations of the elderly, with or without atherosclerosis, because of immunosenescence and an immunosuppressive phenotype if with atherosclerosis- which results in lack of induction of immune memory, non-proliferation, and activation of T and B cells and induced immunity, if any, of a few weeks ( about three). In fact, the lethality of vaccinated elderly people is on the news. The treaty shows that the governments‘ policy of vaccinating young people and the elderly is wrong and exposes them to immediate (including death) and long-term adverse effects. This requires a total change of preventive strategy and effective early care, moving to antiviral allostasis and preventive immunostimulation with the resignation of the public health organizers of the wrong prevention strategy only based on dangerous vaccines, which alter cells’ epigenetic by inducing micro-RNA suppression increasing vulnerability to tumors. In the third part of the treatise the author analyses in-depth the biochemical and immunological (130 pages) the very powerful antiviral and immuno-stimulant, immunogenic properties of an easy diet containing particular natural metabolites and nutraceutical supplements. The diet-induced antiviral allostasis cancels the risk of infection by Sars-COV 2 and variants, and many viruses, suggesting the validity of primary and secondary prevention based on antiviral allostasis and preventive immunostimulation that would have led to the block of onset of the pandemic saving millions of lives-including 150,000 in Italy. “ Anti-viral allostasis” is a word unknown to organizers of public health for epistemological ignorance of the healthy ministerial bits of intelligence and media virologists-immunologists, not only Italians. The treatise theorizes the foundations of the antiviral strategy and preventive immunostimulation, not only for SARS-COV 2, but for many other viruses, and non-communicable diseases such as cancer that share in the first phase, of infection metabolic alterations (1) of SARS-COV 2 and that the World Health Committee is launching in the world as the only possibility to prevent further epidemics. Mass vaccination induces the production of variants. Moreover, mRNA vaccines cause epigenetic alterations that are transmitted to the offspring. These alterations pose serious questions about the present and future effects on the brain and immunity and therefore on vulnerability to learning difficulties and mental health, tumors, and auto-immunity for DNA hybridization by vectorial vaccines. These irrefutable data present situations of obligation to the choice of vaccination and its imposition (Green Pass), the induction to the vaccination of children and adolescents-young, also cause of deaths- as an expression of a real illiterate and insane abuse of power. The treatise is inspired by Person-Centered Medicine. change of paradigm of Medicine and medical science of which the author is the theorist and lecturer at the Medical School of Milan. In the introduction, Prof. Brera presents the conflict between a preventive-therapeutic biotechnological approach to the pandemic, the only necessary tool, and the person-centered change of the medicine and health paradigm. The shift allowed a new definition of health (2011) introduced by the author in WHO but to date not instituted: “ The choice of the best possibilities to be the best human person", and also a political program, basis of the "Charte Mondiale de la Santé" - "The World Health Charter" presented in Milan in 2017 and diffused in the world by the World Health Committee, an organization addressed to spread in the world the Person-Medicine Paradigm after the WHO failure in the COVID-19 prevention, ethical decay, and epistemological delay. (to adhere: www.worldhealthparadigm.net) The book lays the scientific foundations of the world health development program, called the " People and Person-Centered Prevention Program" promoted by the World Health Committee, and promotes the Person-Centered medicine paradigm spreading in public health. This is the exact opposite of health programs centered on "mechanistic-bio technological therapies and preventions", which override man’s fundamental right to freedom to choose what is most appropriate for personal health. This strategy induced a criminal vaccination of children and adolescents, without an epidemiological justification, with certain and even fatal adverse effects, and the dependence of states on health merchants and vaccines, interested in having a mass of chronic sufferers for profit reasons. Members of the consortium of the multinational drug companies were also able to also falsify scientific data to sell, as it appeared from the $250 million penalties given to Smith-Kline and Becham from the FDA in 2014 and present experimental programs of SARS-COV 2 vaccines of limited and wrong validity, omitting the study of adverse effects of vaccines. Other connected books of the same author 1. Person Centered Medicine and Person-Centered Medicine Clinical Method: Clinical results of the first Medicine unitary paradigm teaching and the SARS-COV 2 entry relativity inducing COVID-19 Person-Centered Prevention. 2. Rischio zero per il COVID-19 con la dieta anti virale.: Manuale di alllostasi anti-virale e immunostimolazione preventiva (Italian Edition)
Conference Paper
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1. Syndemic identity of COVID-19 pandemic: the experimental child" 2. Limits of the available anti COVID-19 vaccines 3. Genotoxicity of anti-COVID-19 vaccines
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Background: The pandemic of Coronavirus Disease 2019 (COVID-19) has been a threat to global health. In the US, the Centers for Disease Control and Prevention (CDC) has listed 12 comorbidities within the first tier that increase with the risk of severe illness from COVID-19, including the comorbidities that are common with increasing age (referred to as age-related comorbidities) and other comorbidities. However, the current method compares a population with and without a particular disease (or disorder), which may result in a bias in the results. Thus, comorbidity risks of COVID-19 mortality may be underestimated. Objective: To re-evaluate the mortality data from the US and estimate the odds ratios of death by major comorbidities with COVID-19, we incorporated the control population with no comorbidity reported and assessed the risk of COVID-19 mortality with a comorbidity. Methods: We collected all the comorbidity data from the public health websites of fifty US States and Washington DC (originally accessed on December 2020). The timing of the data collection should minimize bias from the COVID-19 vaccines and new COVID-19 variants. The comorbidity demographic data were extracted from the state public health data made available online. Using the inverse variance random-effects model, we performed a comparative analysis and estimated the odds ratio of deaths by COVID-19 with pre-existing comorbidities. Results: A total of 39,451 COVID-19 deaths were identified from four States that had comorbidity data, including Alabama, Louisiana, Mississippi, and New York. 92.8% of the COVID-19 deaths were associated with a pre-existing comorbidity. The risk of mortality associated with at least one comorbidity combined was 1113 times higher than that with no comorbidity. The comparative analysis identified nine comorbidities with odds ratios of up to 35 times higher than no comorbidities. Of them, the top four comorbidities were: hypertension (odds ratio 34.73; 95% CI 3.63-331.91; p = 0.002), diabetes (odds ratio 20.16; 95% CI 5.55-73.18; p < 0.00001), cardiovascular disease (odds ratio 18.91; 95% CI 2.88-124.38; p = 0.002), and chronic kidney disease (odds ratio 12.34; 95% CI 9.90-15.39; p < 0.00001). Interestingly, lung disease added only a modest increase in risk (odds ratio 6.69; 95% CI 1.06-42.26; p < 0.00001). Conclusion: The aforementioned comorbidities show surprisingly high risks of COVID-19 mortality when compared to the population with no comorbidity. Major comorbidities were enriched with pre-existing comorbidities that are common with increasing age (cardiovascular disease, diabetes, and hypertension). The COVID-19 deaths were mostly associated with at least one comorbidity, which may be a source of the bias leading to the underestimation of the mortality risks previously reported. We note that the method has limitations stemming primarily from the availability of the data. Taken together, this type of study is useful to approximate the risks, which most likely provide an updated awareness of age-related comorbidities.
Article
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The new coronavirus pandemic started in China in 2019. The intensity of the disease can range from mild to severe, leading to death in many cases. Despite extensive research in this area, the exact molecular nature of virus is not fully recognized; however, according to pieces of evidence, one of the mechanisms of virus pathogenesis is through the function of viral miRNAs. So, we hypothesized that SARS‐CoV‐2 pathogenesis may be due to targeting important genes in the host with its miRNAs, which involved in the respiratory system, immune pathways and vitamin D pathways, thus possibly contributing to disease progression and virus survival. Potential miRNA precursors and mature miRNA were predicted and confirmed based on the virus genome. The next step was to predict and identify their target genes and perform functional enrichment analysis to recognize the biological processes connected with these genes in the three pathways mentioned above through several comprehensive databases. Finally, cis‐acting regulatory elements in 5′ regulatory regions were analysed, and the analysis of available RNAseq data determined the expression level of genes. We revealed that thirty‐nine mature miRNAs could theoretically derive from the SARS‐CoV‐2 genome. Functional enrichment analysis elucidated three highlighted pathways involved in SARS‐CoV‐2 pathogenesis: vitamin D, immune system and respiratory system. Our finding highlighted genes' involvement in three crucial molecular pathways and may help develop new therapeutic targets related to SARS‐CoV‐2.
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Vigorous vaccination programs against SARS-CoV-2-causing Covid-19 are the major chance to fight this dreadful pandemic. The currently administered vaccines depend on adenovirus DNA vectors or on SARS-CoV-2 mRNA that might become reverse transcribed into DNA, however infrequently. In some societies, people have become sensitized against the potential short- or long-term side effects of foreign DNA being injected into humans. In my laboratory, the fate of foreign DNA in mammalian (human) cells and organisms has been investigated for many years. In this review, a summary of the results obtained will be presented. This synopsis has been put in the evolutionary context of retrotransposon insertions into pre-human genomes millions of years ago. In addition, studies on adenovirus vector-based DNA, on the fate of food-ingested DNA as well as the long-term persistence of SARS-CoV-2 RNA/DNA will be described. Actual integration of viral DNA molecules and of adenovirus vector DNA will likely be chance events whose frequency and epigenetic consequences cannot with certainty be assessed. The review also addresses problems of remaining adenoviral gene expression in adenoviral-based vectors and their role in side effects of vaccines. Eventually, it will come down to weighing the possible risks of genomic insertions of vaccine-associated foreign DNA and unknown levels of vector-carried adenoviral gene expression versus protection against the dangers of Covid-19. A decision in favor of vaccination against life-threatening disease appears prudent. Informing the public about the complexities of biology will be a reliable guide when having to reach personal decisions about vaccinations.
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It is urgent and important to understand the relationship of the widespread severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) with host immune response and study the underlining molecular mechanism. N⁶-methylation of adenosine (m6A) in RNA regulates many physiological and disease processes. Here, we investigate m6A modification of SARS-CoV-2 gene in regulating host cell innate immune response. Our data show that SARS-CoV-2 virus has m6A modifications which are enriched in the 3’-end of the viral genome. We find that host cell m6A methyltransferase METTL3 depletion decreases m6A levels in SARS-CoV-2 and host genes, and m6A reduction in viral RNA increases RIG-I binding and subsequently enhances downstream innate immune signaling pathway and inflammatory gene expression. METTL3 expression is reduced and inflammatory genes are induced in severe COVID-19 patients. These findings will aid to understand the COVID-19 pathogenesis and help in designing future studies of regulating innate immunity for COVID-19 treatment.
Article
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In this concise review, we summarize the concepts behind mRNA vaccination. We discuss the innate and adaptive immune response generated by mRNA vaccines in different animal models and in humans. We give examples of viral infections where mRNA vaccines have shown to induce potent responses and we discuss in more detail the recent SARS-CoV-2 mRNA vaccine trials in humans.
Article
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Background: The prevalence of true asymptomatic COVID-19 cases is critical to policy makers considering the effectiveness of mitigation measures against the SARS-CoV-2 pandemic. We aimed to synthesize all available research on the asymptomatic rates and transmission rates where possible. Methods: We searched PubMed, Embase, Cochrane COVID-19 trials, and Europe PMC (which covers pre-print platforms such as MedRxiv). We included primary studies reporting on asymptomatic prevalence where: (a) the sample frame includes at-risk population, and (b) there was sufficiently long follow up to identify pre-symptomatic cases. Meta-analysis used fixed effect and random effects models. We assessed risk of bias by combination of questions adapted from risk of bias tools for prevalence and diagnostic accuracy studies. Results: We screened 2,454 articles and included 13 low risk-of-bias studies from seven countries that tested 21,708 at-risk people, of which 663 were positive and 111 were asymptomatic. Diagnosis in all studies was confirmed using a RT-PCR test. The proportion of asymptomatic cases ranged from 4% to 41%. Meta-analysis (fixed effect) found that the proportion of asymptomatic cases was 17% (95% CI: 14% - 20%) overall; higher in aged care 20% (14% - 27%), and lower in non-aged care 16% (13% - 20%). Five studies provided direct evidence of forward transmission of the infection by asymptomatic cases. Overall, there was a 42% lower relative risk of asymptomatic transmission compared to symptomatic transmission (combined Relative Risk: 0.58; 95% CI 0.335-0.994, p=0.047). Discussion: Our estimates of the prevalence of asymptomatic COVID-19 cases and asymptomatic transmission rates are lower than many highly publicized studies, but still sufficient to warrant policy attention. Further robust epidemiological evidence is urgently needed, including in sub-populations such as children, to better understand the importance of asymptomatic cases for driving spread of the pandemic. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Article
This study assesses antispike (anti-S) IgG antibody titers before and after a third BNT162b2 dose (booster) in individuals aged 60 years and older in Israel.
Article
Importance Safety surveillance of vaccines against COVID-19 is critical to ensure safety, maintain trust, and inform policy. Objectives To monitor 23 serious outcomes weekly, using comprehensive health records on a diverse population. Design, Setting, and Participants This study represents an interim analysis of safety surveillance data from Vaccine Safety Datalink. The 10 162 227 vaccine-eligible members of 8 participating US health plans were monitored with administrative data updated weekly and supplemented with medical record review for selected outcomes from December 14, 2020, through June 26, 2021. Exposures Receipt of BNT162b2 (Pfizer-BioNTech) or mRNA-1273 (Moderna) COVID-19 vaccination, with a risk interval of 21 days for individuals after vaccine dose 1 or 2 compared with an interval of 22 to 42 days for similar individuals after vaccine dose 1 or 2. Main Outcomes and Measures Incidence of serious outcomes, including acute myocardial infarction, Bell palsy, cerebral venous sinus thrombosis, Guillain-Barré syndrome, myocarditis/pericarditis, pulmonary embolism, stroke, and thrombosis with thrombocytopenia syndrome. Incidence of events that occurred among vaccine recipients 1 to 21 days after either dose 1 or 2 of a messenger RNA (mRNA) vaccine was compared with that of vaccinated concurrent comparators who, on the same calendar day, had received their most recent dose 22 to 42 days earlier. Rate ratios (RRs) were estimated by Poisson regression, adjusted for age, sex, race and ethnicity, health plan, and calendar day. For a signal, a 1-sided P < .0048 was required to keep type I error below .05 during 2 years of weekly analyses. For 4 additional outcomes, including anaphylaxis, only descriptive analyses were conducted. Results A total of 11 845 128 doses of mRNA vaccines (57% BNT162b2; 6 175 813 first doses and 5 669 315 second doses) were administered to 6.2 million individuals (mean age, 49 years; 54% female individuals). The incidence of events per 1 000 000 person-years during the risk vs comparison intervals for ischemic stroke was 1612 vs 1781 (RR, 0.97; 95% CI, 0.87-1.08); for appendicitis, 1179 vs 1345 (RR, 0.82; 95% CI, 0.73-0.93); and for acute myocardial infarction, 935 vs 1030 (RR, 1.02; 95% CI, 0.89-1.18). No vaccine-outcome association met the prespecified requirement for a signal. Incidence of confirmed anaphylaxis was 4.8 (95% CI, 3.2-6.9) per million doses of BNT162b2 and 5.1 (95% CI, 3.3-7.6) per million doses of mRNA-1273. Conclusions and Relevance In interim analyses of surveillance of mRNA COVID-19 vaccines, incidence of selected serious outcomes was not significantly higher 1 to 21 days postvaccination compared with 22 to 42 days postvaccination. While CIs were wide for many outcomes, surveillance is ongoing.