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Cryptorchidism is a common congenital abnormality encountered in veterinary clinics. The treatment of choice for this condition is a surgical procedure named orchiectomy or orchidectomy, where the retained testicle is removed. Surgical placement and fixation of the cryptorchid testicle into the scrotum, referred to as orchiopexy, is used in humans. However, due to the hereditary nature of cryptorchidism in dogs, this treatment option has not been proposed in veterinary clinics. Two adult Poodle dogs were referred to our research facility for a sperm parameter evaluation check. The two dogs were unilateral cryptorchid dogs treated with orchiopexy before the age of 6 months. Their sperm kinematics and morphology were within normal ranges, and their libido and testicles sizes were normal. Treatment of unilateral cryptorchidism by orchiopexy in dogs before the age of 6 months successfully restored spermatogenic function and sperm quality-related parameters. However, due to the nature of this condition, orchiectomy remains the treatment of choice.
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CASE REPORT
published: 13 October 2021
doi: 10.3389/fvets.2021.750019
Frontiers in Veterinary Science | www.frontiersin.org 1October 2021 | Volume 8 | Article 750019
Edited by:
Marcelo Horacio Miragaya,
University of Buenos Aires, Argentina
Reviewed by:
Maria Alejandra Stornelli,
National University of La
Plata, Argentina
Izhar Hyder Qazi,
Shaheed Benazir Bhutto University of
Veterinary & Animal
Sciences, Pakistan
*Correspondence:
Min Jung Kim
mjbiogen@gmail.com
Specialty section:
This article was submitted to
Animal Reproduction -
Theriogenology,
a section of the journal
Frontiers in Veterinary Science
Received: 30 July 2021
Accepted: 13 September 2021
Published: 13 October 2021
Citation:
Mahiddine FY and Kim MJ (2021)
Case Report: Orchiopexy in Two
Poodle Dogs and Its Effect on Their
Sperm Quality Parameters.
Front. Vet. Sci. 8:750019.
doi: 10.3389/fvets.2021.750019
Case Report: Orchiopexy in Two
Poodle Dogs and Its Effect on Their
Sperm Quality Parameters
Feriel Yasmine Mahiddine and Min Jung Kim*
Department of Research and Development, Mjbiogen Corp., Seoul, South Korea
Cryptorchidism is a common congenital abnormality encountered in veterinary clinics.
The treatment of choice for this condition is a surgical procedure named orchiectomy or
orchidectomy, where the retained testicle is removed. Surgical placement and fixation of
the cryptorchid testicle into the scrotum, referred to as orchiopexy, is used in humans.
However, due to the hereditary nature of cryptorchidism in dogs, this treatment option
has not been proposed in veterinary clinics. Two adult Poodle dogs were referred to our
research facility for a sperm parameter evaluation check. The two dogs were unilateral
cryptorchid dogs treated with orchiopexy before the age of 6 months. Their sperm
kinematics and morphology were within normal ranges, and their libido and testicles sizes
were normal. Treatment of unilateral cryptorchidism by orchiopexy in dogs before the age
of 6 months successfully restored spermatogenic function and sperm quality-related
parameters. However, due to the nature of this condition, orchiectomy remains the
treatment of choice.
Keywords: cryptorchidism, orchiopexy, dog, orchiectomy, sperm
INTRODUCTION
Cryptorchidism is a congenital urological condition characterized by incomplete or absent
testicular descent. In dogs, the testicles pass through the inguinal canal 34 days after birth
and reach their final position in the scrotum on day 35 of life (1). Abnormal abdominal
translocation and transinguinal migration result from incomplete or absent testicular descent due
to abnormalities in the gubernaculums outgrowth and regression or the persistence of cranial
gonadal suspensory ligaments (1,2). Different forms of cryptorchidism have been described based
on whether one or both testicles are involved and where their position is (1,3). Depending on the
side, cryptorchidism can be bilateral or unilateral (1). The latter form is the most common (75%
of the cases), with the right testicle being twice as likely to be retained (4,5). Depending on the
position, high abdominal, low abdominal, or inguinal cryptorchid testicles are the different forms
that can be found (4,6).
It is commonly encountered in small animal veterinary clinics, with an incidence of 1.212.9%
in dogs (4,5) and 1.73.8% in cats (4,5). In dogs, it is an inherited, autosomal recessive trait, with a
higher incidence reported in small breeds than in large breeds (5), especially in breeds such as Boxer,
Cairn Terrier, Chihuahua, English Bulldog, Maltese, Miniature Poodle, Miniature Schnauze, Old
English Sheepdog, Pekingese, Pomeranian, Shetland Sheepdog, and Toy Poodle (7). In general, a
higher incidence of cryptorchidism in purebred dogs than in crossbred dogs has been reported (8).
Mahiddine and Kim Orchiopexy Effects on Sperm Parameters
The treatment of cryptorchidism by orchiopexy in dogs is
a controversial topic, as cryptorchidism is a hereditary trait,
and the risks of testicular neoplasia are still present in these
animals (9). This surgery is no longer performed in veterinary
hospitals, and cryptorchid dogs should not be included in
reproductive programs. Although the treatment of choice for
cryptorchidism is orchiectomy, we recently encountered two
cases of orchiopexy performed in a private clinic. Kawakami et al.
(10) reported that spermatogenesis was preserved after surgery
in young dogs (10). However, no studies have been conducted
on canine sperm morphology and kinematic parameters after
orchiopexy. In this case report, we evaluated the morphological
and kinematic parameters of sperm from two dogs who had
undergone orchiopexy surgery.
CASE PRESENTATION
Two unilateral cryptorchidic Toy Poodle dogs that underwent
orchiopexy were referred to our research facility (Mjbiogen) in
Seoul, South Korea, for the evaluation of their sperm kinematic
and morphological parameters. Orchiopexy was performed at 2
months of age in a private clinic 2 years ago. No information
about the surgical procedure or the location of the cryptorchid
testicles was disclosed, and the owners gave their approval for the
use of these results for publication. After reaching puberty, the
owners reported successful pregnancies and delivery of healthy
offsprings, using semen from these dogs. To assess the effects of
the surgery on sperm quality, we collected semen from these dogs
(n=2), analyzed the sperm quality parameters, and compared
them with those of normal, age-matched toy poodles (n=3)
from the same owner. Statistical analysis was performed using
GraphPad Prism 5.0 (GraphPad, CA, San Diego, USA), and
data were analyzed using one-way analysis of variance (ANOVA)
following by a Tukeys multiple comparison test. All dogs were
fed commercial adult dry food, and water was provided ad
libitum. Semen was obtained by digital manipulation in the
presence of a bitch in estrus, and only the second fraction of
the ejaculate was collected and processed for evaluation in the
laboratory. During collection, libido in the operated dogs was not
diminished nor lower than that of the control dogs. To assess any
difference in the testicles sizes, the lengths of the longitudinal and
transverse axes of the right and left testicles were measured using
a simple measuring flat ruler. The results for each testicle were
transcribed into a table, and the orchiopexy and normal testicles
were compared (Table 1). At palpation, the operated testicle had
TABLE 1 | Lengths of the longitudinal and transverse axes of the right and left testicles in orchiopexy and control dogs.
Parameters Orchiopexy dog 1 Orchiopexy dog 2 Control dogs
Right longitudinal axis (cm) 1.9 2 2.4 ±0.1
Left longitudinal axis (cm) 2.4 3 2.7 ±0.1
Right transverse axis (cm) 1.1 1.3 1.4 ±0.1
Left transverse axis (cm) 1.3 1.2 1.5 ±0.1
no deformation, but in one of the dogs the right testicle was
slightly smaller than the left testicle (Orchiopexy dog 1).
Each collected semen sample (n=3) was diluted with
Tris-extender (1:1 [v/v]—distilled water, Tris (hydroxymethyl)
aminomethane 24 g/L, citric acid 14 g/L, fructose 8 g/L, and
kanamycin sulfate 0.15 g/L; pH 6.6, 290 mOsm) and centrifuged
at 700 ×gfor 1 min. The supernatant was collected and
centrifuged (500 ×g/5 min), and only the pellet was resuspended
in Tris-extender to achieve a concentration of 200 ×106
sperm/ml. Transportation media54% (v/v) Tris-extender, 40%
(v/v) egg yolk, and 6% (v/v) glycerol—was added, and the
samples were stored at 4C to be transported to the laboratory,
1 h away from the breeding facility. Once in the laboratory,
each sample was washed and resuspended in Tris-extender
before proceeding for further analysis. The sperm quality-related
parameters assessed were motility and kinematic parameters,
viability, and morphology parameters, using a computer-assisted
sperm analysis (CASA; Sperm Class Analyzer R
System version
6.4.0.93, Microptic, Barcelona, Spain). The system included a
Nikon Eclipse ci-L microscope (Nikon, Tokyo, Japan) with a
×10 phase-contrast objective. Leja 20-µm chamber slides (Leja,
Gynotec Malden, Nieuw Vennep, the Netherlands) were used
for the analysis, and the frame rate was set at 25 frames/s.
Sperm motility, progressive motility, curvilinear velocity (VCL),
straight-line velocity (VSL), average path velocity (VAP), linearity
(LIN), straightness (VSL/VAP) (STR), wobble VAP/VCL (WOB),
the amplitude of lateral head, and beat cross frequency were
analyzed (Table 2). No differences were observed between the
orchiopexy dogs and the control dogs, indicating that dogs that
underwent orchiopexy surgery have sperm kinematic parameters
within the normal ranges.
Eosin Y staining was used to determine the percentage of
live sperm cells. In brief, the samples were washed, and a
drop of 10 µlfrom the sperm pellet with an equal amount
of 0.5% eosin Y solution was mixed and smeared onto
warm glass slides. The slides were then air-dried, and the
sperm were evaluated. For each stained smear, 200 sperm
were examined with a light microscope (Eclipse Ts 2, Nikon,
Tokyo, Japan) with an oil immersion objective lens (×1000
magnification) (11). The unstained sperm were counted as
alive, and the stained sperm were counted as dead cells. The
results are expressed as the percentage of live sperm cells
(12) (Table 2) and showed no difference between orchiopexy
dogs and control dogs. The sperm acrosome membrane was
analyzed using fluorescein isothiocyanate-conjugated peanut
agglutinin (FITC-PNA), as previously described (13). In brief,
Frontiers in Veterinary Science | www.frontiersin.org 2October 2021 | Volume 8 | Article 750019
Mahiddine and Kim Orchiopexy Effects on Sperm Parameters
TABLE 2 | Kinematic parameters, live cell, and intact acrosome percentages in orchiopexy and control dogs.
Parameters Orchiopexy dog 1 Orchiopexy dog 2 Control dogs
Concentration (M/ml) 355.1 ±167.7 577.1 ±51.9 592.6 ±71.9
Semen volume (ml) 1.8 ±0.4 1.5 ±0.1 0.7 ±0.1
Motility (%) 89.7 ±5.3 99.1 ±0.5 96.8 ±1.4
Progressive motility (%) 49.8 ±9.8 61.4 ±2.4 54.6 ±6.8
VCL (µm/s) 80.5 ±12.9 119.8 ±3.5 103.0 ±9.6
VAP (µm/s) 49.6 ±6.5 64.5 ±1.4 58.9 ±5.5
VSL (µm/s) 34.7 ±3.0 39.6 ±2.4 37.1 ±3.5
LIN (%) 42.2 ±4.7 31.8 ±1.1 34.8 ±1.9
STR (%) 66.2 ±5.6 57.6 ±1.9 59.0 ±2.6
WOB (%) 61.2 ±1.9 53.7 ±0.5 57.2 ±0.9
ALH (µm) 2.1 ±0.4 3.1 ±0.1 2.7 ±0.2
BCF (Hz) 10.4 ±1.1 10.5 ±1.0 10.8 ±0.8
Live cell percentage (%) 75.3 ±8.0 76.8 ±2.5 72.9 ±3.8
Intact acrosome (%) 89.7 ±4.4 93.5 ±3.0 94.6 ±1.1
VCL, curvilinear velocity; VAP, average path velocity; VSL, straight-line velocity; LIN, linearity; STR, straightness (VSL/VAP); WOB, wobble (VAP/VCL); ALH, amplitude of lateral head
displacement; BCF, beat cross frequency.
semen was smeared on glass slides, air-dried, fixed in absolute
methanol, stained, and mounted with anti-fade mounting
medium (VECTASHIELD R
, Vector Laboratories, Burlingame,
CA, USA). The integrity of the sperm acrosome membrane was
analyzed using an epifluorescence phase-contrast microscope
(Eclipse Ts 2, Nikon, Tokyo, Japan) and classified as intact
acrosome (strong green fluorescence) or non-intact acrosome
(partial or no fluorescence) (14) (Table 2 and Figure 1).
Our results showed that acrosome integrity was preserved
in orchiopexy dogs. Diff-Quick staining was used to assess
morphological defects. Each sample was washed and smeared
onto warm glass slides, fixed in methanol, and stained with
eosin as an anionic/acidic dye and with methylene blue as
a cationic dye. The slides were air-dried, and morphology
was assessed using a light microscope (Eclipse Ts 2, Nikon,
Tokyo, Japan) with an oil immersion objective lens (×1,000
magnification). Head defects, droplet, coiled, and bent tail defects
were evaluated in each sample (15) (Table 3). Morphology
parameters were similar in orchiopexy and control dogs,
which shows that orchiopexy at a young age in dogs has no
negative effects on sperm morphology and that spermiogenesis
is preserved.
DISCUSSION
An orchiopexy is the surgical fixation of testicles in the scrotum
(16). It can be performed through open surgery techniques or
laparoscopy (17). The incidence of undescended testis (UDT)
in humans is 1% in male infants, and it is considered as one
of the most common congenital abnormalities in males (18).
However, unlike cryptorchidism in dogs, UDT is corrected with
orchiopexy. The hereditary character of cryptorchidism in dogs
makes it hard, and almost unethical, to perform this surgery
on cryptorchid males (8,19). Moreover, cryptorchidism can be
associated with other congenital abnormalities (8,20), and the
FIGURE 1 | Sperm acrosome stained with fluorescein
isothiocyanate-conjugated peanut agglutinin (FITC-PNA). Sperm with (A) intact
acrosome and (B) non-intact acrosome.
risks of developing a neoplasia in testicles subjected to orchiopexy
are higher (6,9). The aim of this case study is to assess the
sperm parameters in two cryptorchid dogs; however, for all the
aforementioned reasons, orchiopexy should not be suggested by
practicians to cryptorchid dogs owners.
In humans, early orchiopexy (<1 year old) was associated with
significantly higher sperm count and motile sperm in comparison
with cases where orchiopexy was performed later (1–2 years of
age) (21). As orchiopexy is a controversial surgery in veterinary
medicine, the ideal age to perform this surgery has never been
established. In dogs, the final diagnosis of cryptorchidism can
only be made after 6 months of age (22). However, since testicles
Frontiers in Veterinary Science | www.frontiersin.org 3October 2021 | Volume 8 | Article 750019
Mahiddine and Kim Orchiopexy Effects on Sperm Parameters
TABLE 3 | Percentage of morphological defects in orchiopexy and control dogs.
Parameter Orchiopexy dog 1 Orchiopexy dog 2 Control dogs
Morphological defects 2.5 ±1.3 1.0 ±0.3 2.2 ±1.0
are expected to reach their final position around day 35 of life
(23), cryptorchidism can be suspected in pups aged 2 months.
In this case, according to the owners, orchiopexy was
performed on the two Poodle dogs at 2 months of age to ensure a
higher percentage of full recovery of spermatogenetic function.
When the testicles stay in the abdominal cavity, where the
temperature is higher, spermatogenesis is compromised. Since
testicles’ sensitivity to temperature is high, a surgery performed
later could result in the absence of spermatogenesis or the
presence of poor quality sperm with low fertilizing ability (24).
From our own data, a beagle dog with unilateral cryptorchidism
showed motility and progressive motility parameters of 24.7
±29.4% and 29.8 ±22.3%, respectively (unpublished data),
which shows that the sperm kinematic parameters of unilateral
cryptorchid dogs are lower than those of normal dogs.
In humans, males who underwent unilateral orchiopexy at
a young age (before 8 years of age) have a good prognosis for
fertility compared to males who were operated on later or for
both testicles (25). In this case, dogs who underwent unilateral
orchiopexy before the age of 6 months had sperm kinematic and
morphological parameters within the normal range. It should
be noted that Orchiopexy dog 2 sperm parameters, especially
sperm total motility and progressive motility, were slightly higher
than those of the other dogs but were not statistically significant
(p-value >0.05). Morphological defects in Orchiopexy dog 2
sperm samples were also lower than those of the control dogs
and Orchiopexy dog 1 (Table 3). In addition, Orchiopexy dog
1 sperm concentration and acrosome integrity were lower than
those of control dogs and Orchiopexy dog 2, without statistical
significance (p-value >0.05) (Table 2). This difference in sperm
concentration between Orchiopexy dog 1 and the other dogs
could also be attributed to the difference in testicular sizes
(Table 1) (26). From these results, unilateral cryptorchid dogs
treated with early orchiopexy may have a good prognosis for
fertility, and their sperm quality parameters are similar to those
of fertile control dogs.
CONCLUSION
Here, we report two cases of dog orchiopexy in which the
sperm kinematic and morphological parameters were similar to
those from healthy age-matched dogs of the same breed. Like in
humans, orchiopexy at a young age in dogs seems to preserve
sperm quality parameters as our results show that orchiopexy
before the age of 6 months did not alter sperm quality parameters
in these two dogs. This makes dogs as one potential orchiopexy
study model for humans. However, this remains a controversial
surgery due to the hereditary aspect of cryptorchidism and the
risk of testicular neoplasia. The results from this case study
should not encourage dog owners or practitioners to perform it.
Therefore, we recommend veterinarians to warn owners about
this surgery and its dangers and dissuade them from considering
it as a treatment option.
DATA AVAILABILITY STATEMENT
The original contributions presented in the study are included
in the article/supplementary material, further inquiries can be
directed to the corresponding author/s.
ETHICS STATEMENT
Ethical review and approval was not required for the animal study
because it is a clinical case and the owners gave their permission.
Written informed consent was obtained from the owners for the
participation of their animals in this study.
AUTHOR CONTRIBUTIONS
MK participated in collecting owners approval, semen samples,
and revising the manuscript. FM participated in collecting semen
samples and results and drafting and revising the manuscript.
Both authors contributed to the article and approved the
submitted version.
FUNDING
Cooperative research program of Rural Development
Administration (#PJ014786012021).
ACKNOWLEDGMENTS
The authors would like to thank Hee Kee Park for his help
and assistance.
REFERENCES
1. Johnston SD, Root Kustritz MV, Olson PS. Canine and feline theriogenology.
Saunders. (2001).
2. Kersten W, Molenaar GJ, Emmen JM, Van Der Schoot P. Bilateral
cryptorchidism in a dog with persistent cranial testis suspensory ligaments
and inverted gubernacula:report of a case with implications for understanding
normal and aberrant testis descent. J Anat. (1996) 189:171.
3. Griffin B, White S, Kustritz MVR. Disorders of sexual development and
common reproductive pathologies. High-Quality, High-Volume Spay and
Neuter and Other Shelter Surgeries. (2020) 27–51. doi: 10.1002/9781119646
006.ch2
Frontiers in Veterinary Science | www.frontiersin.org 4October 2021 | Volume 8 | Article 750019
Mahiddine and Kim Orchiopexy Effects on Sperm Parameters
4. Yates D, Hayes G, Heffernan M, Beynon R. Incidence of cryptorchidism
in dogs and cats. Veter Record. (2003) 152:502–4. doi: 10.1136/vr.152.
16.502
5. Tobias KM, Johnston SA. Veterinary surgery: small animal: 2 volume set.
Elsevier Health Sciences. (2013).
6. Mattos MRF, Simões-Mattos L, Domingues SFS. Cryptorchidism in dog.
Ciência Animal. (2000) 10:61–70.
7. Spangenberg C. Canine cryptorchidism: a concise review of its origin,
diagnosis and treatment caroline spangenberg. Biol, Eng, Med Sci Rep. (2021)
7:1–3. doi: 10.5530/bems.7.1.1
8. Khan FA, Gartley CJ, Khanam A. Canine cryptorchidism: an update. Reprod
Domest Animals. (2018) 53:1263–70. doi: 10.1111/rda.13231
9. Higgins M, Smith DE, Gao D, Wilcox D, Cost NG, Saltzman AF. The impact
of age at orchiopexy on testicular cancer outcomes. World J Urol. (2020)
38:2531–6. doi: 10.1007/s00345-019-03034-9
10. Kawakami E, Tsutsui T, Yamada Y, Ogasa A, Yamauchi M. Spermatogenic
function in cryptorchid dogs after orchiopexy. Japanese J Veter Sci. (1988)
50:227–35. doi: 10.1292/jvms1939.50.227
11. Eliasson R, Treichl L. Supravital staining of human spermatozoa. Fertil Steril.
(1971) 22:134–7. doi: 10.1016/S0015-0282(16)38049-9
12. O’Connell M, McClure N, Lewis SE. The effects of cryopreservation on
sperm morphology, motility and mitochondrial function. Hum Reprod. (2002)
17:704–9. doi: 10.1093/humrep/17.3.704
13. MahiddineFY, Kim JW, Qamar AY, Ra JC, Kim SH, Jung EJ, et al. Conditioned
medium from canine amniotic membrane-derived mesenchymal stem cells
improved dog sperm post-thaw quality-related parameters. Animals. (2020)
10:1899. doi: 10.3390/ani10101899
14. Ren F, Fang Q, Feng T, Li Y, Wang Y, Zhu H. Lycium barbarum and
Laminaria japonica polysaccharides improve Cashmere goat sperm quality
and fertility rate after cryopreservation. Theriogenology. (2019) 129:29–36.
doi: 10.1016/j.theriogenology.2019.02.011
15. Kruger TF, Ackerman SB, Simmons KF, Swanson RJ, Brugo SS, Acosta AA. A
quick, reliable staining technique for human sperm morphology. Arch Androl.
(1987) 18:275–7. doi: 10.3109/01485018708988493
16. Rajfer J. Technique of orchiopexy. Urol Clin North Am. (1982) 9:421–
7. doi: 10.1016/S0094-0143(21)01357-4
17. Shirazi M, Safavi S, Makarem A, Malekmakan L. Comparison between
processus vaginalis sac tightening technique and the conventional technique
in orchiopexy surgery over 10 years. Research and reports in urology. (2020)
12:129. doi: 10.2147/RRU.S237824
18. Yang Z, Li S, Yin J, Bao J, Zeng H, Xu W, et al. A prediction model for risk
factors of testicular atrophy after orchiopexy in children with undescended
testis. Transl Pediatr. (2021) 10:882. doi: 10.21037/tp-20-473
19. Niemand HG, Suter PF. Pratique de la clinique canine. Vigot. (1992).
20. Pendergrass TW, Hayes Jr HM. Cryptorchism and related defects in
dogs:epidemiologic comparisons with man. Teratology. (1975) 12:51–
5. doi: 10.1002/tera.1420120107
21. Chan E, Wayne C, Nasr A. Ideal timing of orchiopexy:a systematic
review. Pediatr Surg Int. (2014) 30:87–97. doi: 10.1007/s00383-013-
3429-y
22. Memon M. Tibary A. Canine and feline cryptorchidism. Ivis (En línea). (2001).
23. Goericke-Pesch S, Wehrend A. Cryptorchidism in dogs and cats-definition,
prevalence, diagnosis and therapy. Praktische Tierarzt. (2013) 94:974–81.
24. Setchell B. The effects of heat on the testes of mammals. Animal Reproduction
(AR). (2018) 3:81–91.
25. Trsinar B, Muravec UR. Fertility potential after unilateral
and bilateral orchidopexy for cryptorchidism. World
J Urol. (2009) 27:513–9. doi: 10.1007/s00345-009-
0406-0
26. Olar T, Amann R, Pickett B. Relationships among testicular size,
daily production and output of spermatozoa, and extragonadal
spermatozoal reserves of the dog. Biol Reprod. (1983) 29:1114–
20. doi: 10.1095/biolreprod29.5.1114
Conflict of Interest: The authors were employed by company Mjbiogen Corp.
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Background Undescended testis (UDT) is a common congenital urogenital anomaly that is treated by orchiopexy. We aimed to introduce patent processus vaginalis (PPV) sac tightening (PVST) technique and compare it to the conventional technique. Methods We retrospectively studied all the operated UDT patients during 10 years. In the conventional technique, it was necessary to ligate PPV sac after being peeled off from the spermatic cord. PVST was dissected longitudinally from the two sides of where the PPV sac wall was attached to the spermatic cord till the proximal part, and only a narrow thin layer sticking to the spermatic cord was left and the proximal PVV sac opening was tightened as much as possible with vicryl suture at the internal inguinal ring level. The significance level was <0.05. Results Of 821 orchiopexy (mean age 24.5±24.2 months), 36.3% were done by conventional and 63.7% by PVST technique. Hematoma, edema, hydrocele, and wound infection were lower in the PVST technique, but it was not significant (p>0.05). Testicular atrophy and operation time were significantly lower in the PVST than the conventional technique (p<0.001). Conclusion The orchiopexy PVST technique has lower complications and seems to be easier, faster and safer than the conventional technique.
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Purpose To estimate how many boys with UDT must undergo orchiopexy to prevent one case of TC, one death from TC and one exposure to TC treatment beyond radical orchiectomy as compared to being treated at an older age. Methods This retrospective study utilized data from a 2007 Swedish study of males who underwent orchiopexy for UDT (Pettersson et al.). TC incidence for boys undergoing orchiopexy for UDT was assessed based on the age at orchiopexy (0–6 years, 7–9 years, 10–12 years, 13–15 years). The incidence of TC in each age cohort was calculated and used to determine the number needed to treat (NNT) for each age group using assumptions based on published TC outcomes. Results For an index patient ≤ 6 years, 372 boys need to undergo orchiopexy to prevent a single case of TC, 1488 boys to prevent exposure to TC therapy beyond radical orchiectomy, and 5315 boys to prevent a single TC-related death compared to treatment at an older age. Conclusion While there is evidence supporting benefits of early orchiopexy, the NNT to affect TC outcomes is very high. Even those with delayed orchiopexies have low risk for TC poor outcomes. This information can be used when counseling patients and families faced with UDT about the risks related to TC, especially with comorbidities.
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Contents This review article is aimed at providing an update on canine cryptorchidism based on the research studies published in this area over the last 26 years. Although a majority of these research studies were directed at investigating the genetic and molecular basis of cryptorchidism, the mechanisms underlying this problem in dogs are still not clear. Future studies using advanced genomic and molecular approaches and including a larger number of dogs of multiple breeds are required to further our understanding of canine cryptorchidism. Diagnosis and treatment of cryptorchidism in dogs have not received much attention. Research advancements include investigation of ultrasonography and anti‐Müllerian hormone assay as potential diagnostic methods. Advancements in treatment include the use of laparoscopic‐assisted cryptorchidectomy. Based on the available literature, this method appears to be the best for removal of abdominal cryptorchid testes from dogs. However, future studies that compare laparoscopic‐assisted cryptorchidectomy with the traditional approaches are required for a more conclusive determination of the best method for cryptorchidectomy in dogs.
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The ideal management of cryptorchidism is a highly debated topic within the field of pediatric surgery. The optimal timing of orchiopexy is particularly unclear, as existing literature reports mixed recommendations. The aim of this study was to determine, based on a systematic review, the most favorable age at which orchiopexy should be performed. We conducted a systematic search of MEDLINE, Embase, CINAHL, and the Cochrane Library to find relevant articles. Two researchers quality assessed each study using the following tools: AMSTAR (systematic reviews), Jadad (RCTs), and MINORS (non-RCTs). We developed a conclusion based on the highest quality studies. We found one relevant systematic review, one RCT, and 30 non-RCTs. Fertility potential was greatest when orchiopexy was performed before 1 year of age. Additionally, orchiopexy before 10-11 years may protect against the increased risk of testicular cancer associated with cryptorchidism. Orchiopexy should not be performed before 6 months of age, as testes may descend spontaneously during the first few months of life. The highest quality evidence recommends orchiopexy between 6 and 12 months of age. Surgery during this timeframe may optimize fertility potential and protect against testicular malignancy in children with cryptorchidism.