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XVIII МЕЖДУНАРОДНАЯ МОЛОДЁЖНАЯ ЭКОЛОГИЧЕСКАЯ КОНФЕРЕНЦИЯ «ЧЕЛОВЕК И БИОСФЕРА». БУДУЩЕЕ ЗАВИСИТ ОТ НАС [XVIII INTERNATIONAL YOUTH ECOLOGICAL CONFERENCE "MAN AND BIOSPHERE": FUTURE DEPENDS ON US]. Chief-Editor N.S. Vshivkova

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Abstract

The Abstract Book contains materials of the XVIII International Youth Ecological Conference "Man and Biosphere". The results of original research works and reviews on regional and global problems of ecology, biodiversity of flora and fauna, in various areas of practical ecology, ecotourism, ecological education and upbringing are presented. The book is intended for representatives of public environmental organizations, teachers and students of secondary and higher education institutions, for all who are interested in environmental and environmental issues.
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Article
Background: Distinguishing among species in the genus Lepista is difficult because of their similar morphologies. Methods: To identify a suitable DNA barcode for identification of Lepista species, we assessed the following five regions: internal transcribed spacer (ITS), the intergenic spacer (IGS), nuclear ribosomal RNA subunit, mitochondrial small subunit rDNA, and tef1. A total of 134 sequences from 34 samples belong to eight Lepista species were analyzed. The utility of each region as a DNA barcode was assessed based on the success rates of its PCR amplification and sequencing, and on its intra- and inter-specific variations. Results: The results indicated that the ITS region could distinguish all species tested. We therefore propose that the ITS region can be used as a DNA barcode for the genus Lepista. In addition, a phylogenetic tree based on the ITS region showed that the tested eight Lepista species, including two unrecognized species, formed eight separate and well-supported clades.
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