Article

The Role of Forensic Anthropological Techniques in Identifying America's War Dead from Past Conflicts

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  • Defense POW/MIA Accounting Agency
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Abstract

The Scientific Analysis Directorate of the U.S. Department of Defense's (DoD) Defense POW/MIA Accounting Agency (DPAA) is a unique entity within the U.S. Government. This agency currently houses the world's largest, accredited skeletal identification laboratory in the world, in terms of the size of the scientific staff, global mission, and number of annual identifications. Traditional forensic anthropology is used for the formation of a biological profile (biological sex, stature, population affinity/ancestry, and age) as well as trauma and pathologies that may be compared with historical records and personnel files. Since World War II, various scientists associated with DoD have conducted base‐line research in support of the identification of U.S. war dead, including, but not limited to, histology, the use of chest radiography and clavicle comparison, and statistical models to deal with commingling issues. The primary goal of the identification process of the Scientific Analysis Directorate is to use all available historical, field, and forensic methods to establish the most robust and defendable identification as scientifically and legally possible. This article is categorized under: Forensic Anthropology > Age Assessment Forensic Anthropology > Sex Assessment Forensic Anthropology > Ancestry Determination

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... Forensic Odontology and dental records are further sources of identifying information. In mass casualty cases with victims of similar biological profiles, dental conditions can be more varied, allowing greater insight into individual identities than the biological profile alone (Belcher et al., 2021). ...
... Antemortem and postmortem dental charts may be compared, with matches contributing to a positive identification. Dental charts are common in individual personnel files, documenting any dental anomalies (i.e., cavities, crowns, missing teeth, etc.) noted via dental chart, although x-rays were extremely uncommon if nonexistent throughout WWII (Belcher et al., 2021;Shiroma, 2016). In a study on identifications of victims of the USS Oklahoma, Shiroma notes that common discrepancies such as differences in documentation of surface restorations, antemortem tooth loss, interproximal restorations, errors in the dental charts themselves, and dental restorations performed on deciduous teeth may all be factors that may affect the accuracy of identification (Shiroma, 2020). ...
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... Forensic anthropology played a very important role in cases of human rights violations both in historical and forensic context. The field's contribution is especially evident in cases of personal identification of US soldiers that died during World War II [12]. Another example are the results of search, recovery and analysis that lead to identification of the Second World War heroes of different nationalities that died across the present country's borders [13]. ...
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This edition of the internationally acclaimed guide to forensic stable isotope analysis uses real-world examples to bridge discussions of the basic science, instrumentation and analytical techniques underlying forensic isotope profiling and its various technical applications. Case studies describe an array of applications, many of which were developed by the author himself. They include cases in which isotope profiling was used in murder, and drugs-related crime investigations, as well as for pharmaceutical and food authenticity control studies. Updated with coverage of exciting advances occurring in the field since the publication of the 1st edition, this 2nd edition explores innovative new techniques and applications in forensic isotope profiling, as well as key findings from original research. More than a simple update, though, this edition has been significantly revised in order to address serious problems that can arise from non-comparable and unfit-for-purpose stable isotope data. To that end, Part II has been virtually rewritten with greater emphasis now being placed on important quality control issues in stable isotope analysis in general and forensic stable isotope analysis in particular. Written in a highly accessible style that will appeal to practitioners, researchers and students alike Illustrates the many strengths and potential pitfalls of forensic stable isotope analysis Uses recent case examples to bridge underlying principles with technical applications Presents hands-on applications that let experienced researchers and forensic practitioners match problems with success stories Includes new chapters devoted to aspects of quality control and quality assurance, including scale normalisation, the identical treatment principle, hydrogen exchange and accreditation Stable Isotope Forensics, 2nd Edition is an important professional resource for forensic scientists, law enforcement officials, public prosecutors, defence attorneys, forensic anthropologists and others for whom isotope profiling has become an indispensable tool of the trade. It is also an excellent introduction to the field for senior undergraduate and graduate forensic science students.
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This chapter provides some background and applications of stable isotope analysis in the context of humanitarian forensic science to illustrate the contribution that isotopic profiling can make to an investigation of an unidentified decedent. It also describes a few of the isotopic tools and data resources currently available to forensic investigators. Human tissues can be categorized into tissues with a longer‐term memory such as bone and teeth and tissues with a shorter‐term memory such as hair and nail. Isotopic profiling of human hair has received a good deal of attention in the past decade, and a rich repository of published knowledge exists on the particular challenge that is H isotope abundance analysis of hair. The isotopic profiles of human tissues such as bone, teeth, hair and nail hold great potential for forensic investigations by providing valuable information regarding an unidentified decedent's life history that cannot be obtained by any other analytical method.
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Rationale Strontium isotope ratios (⁸⁷Sr/⁸⁶Sr) in human fingernail keratin tissues have been underexplored for region of origin and travel history reconstruction studies. Here we investigated ⁸⁷Sr/⁸⁶Sr ratios in fingernail keratin to establish baseline measurements in a resident group and to examine how ⁸⁷Sr/⁸⁶Sr ratios changed with relocation. Methods Fingernail clippings were collected from resident (n=10) and non‐resident/traveler groups (n=4 and n=4) that were part of a larger study in Salt Lake City (UT, USA) from 2015 to 2016. Strontium abundance and ⁸⁷Sr/⁸⁶Sr ratios were determined via MC‐ICP‐MS. ⁸⁷Sr/⁸⁶Sr and oxygen (δ¹⁸O) isotope ratios from the traveler participants were compared to examine temporal patterns. Results Strontium abundance and ⁸⁷Sr/⁸⁶Sr ratios in fingernails from the resident group established a baseline against which we could evaluate potential differences in non‐resident/traveler groups. Resident ⁸⁷Sr/⁸⁶Sr ratios remained constant over the study period and were consistent with previously measured tap waters for the area. ⁸⁷Sr/⁸⁶Sr ratio changes in non‐resident/traveler groups were rapid and reflected the current location of the individual within 4‐5 weeks of arrival. Lastly, δ¹⁸O and ⁸⁷Sr/⁸⁶Sr ratios of the same fingernail clippings did not exhibit similar temporal patterns, since fingernail δ¹⁸O ratios required more time to attain values characteristic of the new environment. Conclusion Our findings suggest that strontium is incorporated into fingernail tissues differently from hair and this could be advantageous to forensic investigations. We found that ⁸⁷Sr/⁸⁶Sr and δ¹⁸O ratios of the same fingernail clippings revealed two different time points reflecting an individual's residence over a short (4‐5 weeks, ⁸⁷Sr/⁸⁶Sr ratios) and long‐term (3‐5 months, δ¹⁸O values) time scales. It is likely that the ⁸⁷Sr/⁸⁶Sr ratios of fingernail clippings reflect exogenous signals that are incorporated through bathing waters and that these signals change rapidly with movement to a new location. Our results may aid future forensic studies in the determination of region of origin in unidentified remains.
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Mass fatality events can result in the intermixing, or commingling, of human remains. Commingling of human remains presents an added challenge to all phases of the forensic process. As the number of individuals increases, so does the complexity of the forensic investigation and the skills needed for case resolution. In Recovery, Analysis, and Identification of Commingled Human Remains, top professionals illustrate successful techniques for sorting and determining the number of individuals, the role of DNA, ethical considerations and data management. Instrumental to the forensic community, Recovery, Analysis, and Identification of Commingled Human Remains provides case examples and an in depth review of experiences, methods, and research related to commingling. © 2008 Humana Press, a part of Springer Science+Business Media, LLC. All rights reserved.
Article
As of August 2014, the Joint POW/MIA Accounting Command has identified the remains of 1980 previously unknown U.S. service members; 280 were from the Korean War. To determine the accuracy and completeness of the available antemortem (AM) dental records, a review of the AM/postmortem (AM/PM) dental record comparisons from 233 Forensic Odontology Reports written in support of remains identified from the Korean War was performed. Seventy-two AM/PM comparisons resulted in exact dental chartings while 161 contained discrepancies which were explainable. Explainable discrepancies include undocumented treatment (103), incorrectly charted third molars as missing (82), differing opinions of specific molars present/missing (20), and erroneous treatment documentation and/or misidentification of teeth present/missing (22, other than molars). Reassessment has revealed varying levels of completeness for our available AM dental records, the need to thoroughly review our computerized comparisons, adjust our comparisons to include molar pattern variations/third molars, and updating our database comparison program.
Chapter
Assemblages of commingled human remains present special problems for the identification process. Complete biological profiles of individuals cannot be developed until the remains have been segregated into individuals. Cause and manner of death cannot be fully evaluated without access to segregated, complete skeletons. Segregation is also a goal for reasons extending beyond the need for comprehensive analysis, as in forensic applications when remains must be returned to next of kin for disposition. Although the presence of soft tissue can, in some cases, usefully inform the process (e.g., hair color and texture, skin tone), most of the information used to resolve commingling is obtained from the skeleton. Charles Snow (1948) provided an early comprehensive overview of methods for sorting commingled skeletons that is still relevant today, although it predates some important new technologies such as DNA analysis. The primary categories of information used to sort commingled remains are age, articulation, visual pair matching, size (osteometric sorting), robusticity (“build”), taphonomy, and DNA profile data. Osteometric sorting is but one of the many tools available to the anthropologist for sorting commingled remains.
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This is a comprehensive and accessible overview of what is known about the structure and mechanics of bone, bones, and teeth. In it, John Currey incorporates critical new concepts and findings from the two decades of research since the publication of his highly regarded The Mechanical Adaptations of Bones. Crucially, Currey shows how bone structure and bone's mechanical properties are intimately bound up with each other and how the mechanical properties of the material interact with the structure of whole bones to produce an adapted structure. For bone tissue, the book discusses stiffness, strength, viscoelasticity, fatigue, and fracture mechanics properties. For whole bones, subjects dealt with include buckling, the optimum hollowness of long bones, impact fracture, and properties of cancellous bone. The effects of mineralization on stiffness and toughness and the role of microcracking in the fracture process receive particular attention. As a zoologist, Currey views bone and bones as solutions to the design problems that vertebrates have faced during their evolution and throughout the book considers what bones have been adapted to do. He covers the full range of bones and bony tissues, as well as dentin and enamel, and uses both human and non-human examples. Copiously illustrated, engagingly written, and assuming little in the way of prior knowledge or mathematical background, Bones is both an ideal introduction to the field and also a reference sure to be frequently consulted by practicing researchers.
Chapter
The mission of the Joint POW/MIA Accounting Command (JPAC) is to conduct global search, recovery, and laboratory operations to identify unaccounted-for Americans from past conflicts in order to support the Department of Defense’s personnel accounting efforts. Currently, JPAC focuses mainly on World War II, the Korean War, and the Vietnam War. Of these major conflicts, the Korean War generates about half or more of JPAC’s identifications each year. There are multiple ways JPAC gets access to the unaccounted-for American remains from the Korean War, such as exhumations of the Korean War unknown remains from the National Memorial Cemetery of the Pacific or recovery missions in North Korea. In this chapter, we focus on a large-scale commingled assemblage of missing Americans from the Korean War called “the Korea 208” (K208) that the United States government received from the North Korean government in the early 1990s. The goal of this chapter is to present the analytical procedure of the K208 assemblage that originally contained as many as 600 commingled individuals.
Article
Identification of Pathological Conditions in Human Skeletal Remains provides an integrated and comprehensive treatment of pathological conditions that affect the human skeleton. There is much that ancient skeletal remains can reveal to the modern orthopaedist, pathologist, forensic anthropologist, and radiologist about the skeletal manifestations of diseases that are rarely encountered in modern medical practice. Beautifully illustrated with over 1,100 photographs and drawings, this book provides essential text and materials on bone pathology, which will improve the diagnostic ability of those interested in human dry bone pathology. It also provides time depth to our understanding of the effect of disease on past human populations.
Article
The Armed Forces DNA Identification Laboratory (AFDIL) is one of the leading laboratories in the world for the processing of degraded skeletal remains. Extended efforts have been made to develop protocols and standards that will hold up to the intense scrutiny of both the scientific world and the U.S. legal system. Presented in this paper are the specifics of the in-house systems and procedures that have allowed AFDIL to streamline the processing of degraded skeletal remains and family references for mitochondrial DNA (mtDNA) analysis. These include the development of our in-house bioinformatics systems by which every package and sample that passes through the laboratory is tracked; protocols designed specifically for both questioned and reference samples; and the difficulties inherent in this type of organization. Two case studies presented involve one of ancient remains and one on the recent event of September 11, 2001. Finally, future directions available to both AFDIL and the DNA analysis community as a whole are discussed. Copyright © 2004 Central Police University.
Article
During the Korean War, the Office of the Quartermaster General's Graves Registration Service (GRS) was responsible for the recovery, processing, identification, and repatriation of US remains. In January 1951, the GRS established a Central Identification Unit (CIU) at Kokura, Japan. At the Kokura CIU, postmortem dental examinations were performed by the dental technicians. Thirty-nine postmortem dental examinations performed at the CIU were compared to the findings documented in the Forensic Odontology Reports written at the JPAC Central Identification Laboratory (CIL). Differences were noted in 20 comparisons (51%). The majority of the discrepancies was considered negligible and would not alter the JPAC decision to disinter a set of unknown remains. Charting discrepancies that were considered significant included the occasional failure of the Kokura technicians to identify teeth with inter-proximal or esthetic restorations and the misidentification of a mechanically prepared tooth (i.e., tooth prepared for a restoration) as a carious surface. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.
Article
Diet is a leading modifiable risk factor for chronic disease, but it remains difficult to measure accurately due to the error and bias inherent in selfreported methods of diet assessment. Consequently, there is a pressing need for more objective biomarkers of diet for use in health research. The stable isotope ratios of light elements are a promising set of candidate biomarkers because they vary naturally and reproducibly among foods, and those variations are captured in molecules and tissues with high fidelity. Recent studies have identified valid isotopic measures of short- and long-term sugar intake, meat intake, and fish intake in specific populations. These studies provide a strong foundation for validating stable isotopic biomarkers in the general US population. Approaches to improve specificity for specific foods are needed; for example, by modeling intake using multiple stable isotope ratios or by isolating and measuring specific molecules linked to foods of interest. Expected final online publication date for the Annual Review of Nutrition Volume 35 is July 17, 2015. Please see http://www.annualreviews.org/catalog/pubdates.aspx for revised estimates.
Article
The objective of this paper is to compare patterns of osteon organization in human and nonhuman bone. A linear organization of Haversian systems in nonhuman bone, where osteons line up in rows, has been reported but has not been quantified. The present research provides a quantitative examination of this observation through a comparative analysis of the femoral midshaft from human and nonhuman bone. Femoral midshaft thin sections from 60 humans were compared to femoral midshaft sections from nine sheep and six miniature swine. The presence or absence of osteon banding was recorded and, if present, described. Results indicate that 2 out of 60 human sections and 5 out of 15 nonhuman sections exhibit osteon banding (chi2 = 9.46; p < 0.01). Further, the type of banding present in the human and nonhuman samples is easily distinguished, indicating that human and nonhuman bone can be distinguished where handing is present in this study.
Chapter
This review covers isotope ratio and trace element analyses of human and animal hair. The topic areas include hair structure and its survival in archaeological and forensic contexts, isotope ratios of animal hair, both in controlled, experimental situations and through collections from wild populations, anthropological studies on modern and historically collected primate and human hair, health and medical applications of hair isotope and trace element analysis, applications to archaeological hair samples, the use of isotope ratios from hair in forensic investigations, and finally a discussion of geography and temporal dynamics in hair oxygen isotope ratios.
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Ancestry assessments using cranial morphoscopic traits currently rely on subjective trait lists and observer experience rather than empirical support. The trait list approach, which is untested, unverified, and in many respects unrefined, is relied upon because of tradition and subjective experience. Our objective was to examine the utility of frequently cited morphoscopic traits and to explore eleven appropriate and novel methods for classifying an unknown cranium into one of several reference groups. Based on these results, artificial neural networks (aNNs), OSSA, support vector machines, and random forest models showed mean classification accuracies of at least 85%. The aNNs had the highest overall classification rate (87.8%), and random forests show the smallest difference between the highest (90.4%) and lowest (76.5%) classification accuracies. The results of this research demonstrate that morphoscopic traits can be successfully used to assess ancestry without relying only on the experience of the observer.
Book
Forensic Anthropology: Current Methods and Practice-winner of a 2015 Textbook Excellence Award (Texty) from The Text and Academic Authors Association-approaches forensic anthropology through an innovative style using current practices and real case studies drawn from the varied experiences, backgrounds, and practices of working forensic anthropologists. This text guides the reader through all aspects of human remains recovery and forensic anthropological analysis, presenting principles at a level that is appropriate for those new to the field, while at the same time incorporating evolutionary, biomechanical, and other theoretical foundations for the features and phenomena encountered in forensic anthropological casework. Attention is focused primarily on the most recent and scientifically valid applications commonly employed by working forensic anthropologists. Readers will therefore learn about innovative techniques in the discipline, and aspiring practitioners will be prepared by understanding the necessary background needed to work in the field today. Instructors and students will find Forensic Anthropology: Current Methods and Practice comprehensive, practical, and relevant to the modern discipline of forensic anthropology. Winner of a 2015 Most Promising New Textbook Award from the Text and Academic Authors Association Focuses on modern methods, recent advances in research and technology, and current challenges in the science of forensic anthropology Addresses issues of international relevance such as the role of forensic anthropology in mass disaster response and human rights investigations Includes chapter summaries, topicoriented case studies, keywords, and reflective questions to increase active student learning.
Article
This paper describes a computerized clavicle identification system primarily designed to resolve the identities of unaccounted-for U.S. soldiers who fought in the Korean War. Elliptical Fourier analysis is used to quantify the clavicle outline shape from skeletons and postero-anterior antemortem chest radiographs to rank individuals in terms of metric distance. Similar to leading fingerprint identification systems, shortlists of the top matching candidates are extracted for subsequent human visual assessment. Two independent tests of the computerized system using 17 field-recovered skeletons and 409 chest radiographs demonstrate that true-positive matches are captured within the top 5% of the sample 75% of the time. These results are outstanding given the eroded state of some field-recovered skeletons and the faintness of the 1950's photofluorographs. These methods enhance the capability to resolve several hundred cold cases for which little circumstantial information exists and current DNA and dental record technologies cannot be applied.
Article
During the Korean War, the American Graves Registration Service, U.S. Army Quartermaster Corps, developed an innovative system to recover, identify, and repatriate deceased U.S. servicemen. In doing so, the U.S. armed forces returned their dead to the United States during major combat operations for the first time in military history. This article describes and analyzes the handling of the Korean War dead. It concludes that the wartime program exemplified the country's adaptation to limited war during a time of prosperous insecurity.
Article
This study is novel in that it is the first of its kind to compile a reference sample of isotopic values associated with known natal regions to be utilized in forensic work. Stable isotopes of carbon, oxygen, strontium, and lead were examined to determine if natal origins could be assessed isotopically between Southeast Asian and American dental remains as well as regionally within the United States. Teeth believed to be of East Asian origin were compared to the extracted third molars of recent American dental patients. Living subjects completed surveys detailing physiological, behavioral, and residential information that affect isotope values. The least squares means for all isotope values examined exhibited significant differences between the East Asian and American cohorts. Based on this information, a discriminant function was created that correctly classified individuals, through resubstitution and cross-validation, as belonging to one of these two groups by 95% or better. American strontium values displayed a distinct trend toward homogenization, with the mean value for Sr87/Sr86 varying only slightly from that of seawater. In order to identify natal origin among Americans, nine regions were created within the United States based on O18 values. Good discrimination was noted between the mountain states and the southern states. A discriminant function analysis proved disappointing though, and additional sampling from most states is needed to improve the statistical robusticity of the model. The results of this study will have wide-reaching effects across the medico-legal spectrum. This body of research will serve as the foundation for a database of modern, human, geolocational isotope values that will assist not only in the identification of fallen servicemen and women, but in the identification of victims of mass fatality incidents, undocumented aliens who perish attempting entry into the U.S., and local skeletal "Jane and John Doe" cases.