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Biophoton Imaging Evaluation of the Process of Rheumatoid Arthritis in Rats

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https://www.scirp.org/journal/ns Natural Science, 2021, Vol. 13, (No. 10), pp: 451-456
https://doi.org/10.4236/ns.2021.1310037 451 Natural Science
Biophoton Imaging Evaluation of the Process of
Rheumatoid Arthritis in Rats
Chengming Xia1,2, Jinzhong Li1,2, Yi Yue1,2, Linhua Chen1,2, Jiapei Dai1,2
1Wuhan Institute for Neuroscience and Neuroengineering (WINN), South-Central University for Nationalities,
Wuhan, China; 2Department of Neurobiology, College of Life Sciences, South-Central University for Nationalities,
Wuhan, China
Correspondence to: Jiapei Dai,
Keywords: Rheumatoid Arthritis, Freunds Adjuvant, Biophoton, Biophoton Imaging, Rat
Received: August 24, 2021 Accepted: October 5, 2021 Published: October 8, 2021
Copyright © 2021 by author(s) and Scientific Research Publishing Inc.
This work is licensed under the Creative Commons Attribution-NonCommercial International License (CC BY-NC 4.0).
http://creativecommons.org/licenses/by-nc/4.0/
ABSTRACT
Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly
causes the destruction of small joints. The development of this disease is a relatively secret
and repeated process, and therefore early diagnosis and evaluation of the disease is usually
difficult. In this study, an arthritis model was successfully induced by injecting complete
Freunds adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injec-
tion of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accom-
panied by more sensitivity to thermal stimulation. Using the ultraweak biophoton imaging
system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7
days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats
showed significantly higher biophoton emissions compared with the control group. The
results of this study may provide a basis for further research and development of early di-
agnosis and assessment of lesion progression of rheumatoid arthritis.
1. INTRODUCTION
Rheumatoid arthritis (RA) is the most common chronic inflammatory disease [1], and its main clini-
cal manifestations are symmetry persistent joint swelling and pain. It is also considered a syndrome, in-
cluding extraarticular manifestations, such as rheumatoid nodules, pulmonary involvement or vasculitis,
and systemic complications. With the progress of disease course, patients with RA will have limited joint
function due to joint pain and stiffness [2-6], however, early diagnosis and evaluation of the disease process
are usually difficult, and comprehensive evaluation needs to be combined with multiple detection me-
thods. Therefore, it is of great clinical value to establish a simple technique for early diagnosis and evalua-
tion of disease progress.
Open Access
https://doi.org/10.4236/ns.2021.1310037 452 Natural Science
Ultraweak photon emission (UPE), also known as biophoton, exists in almost all organisms with a
spectral range of 200 - 800 nm, and an intensity of 102 - 103 photons/(cm2·s) [7-10]. Biophoton emissions
are directly related to the free radical activity in the process of oxidative metabolism, as well as the physio-
logical and pathological changes of the body, whereas the process of inflammation is often accompanied
by the increase of free radical activity. Therefore, the detection of biophoton emission has a certain value
in the pathological diagnosis in animals and plants [11-16]. In this study, by detecting and analyzing the
intensity of biophoton emissions in the toe joint area of lower limbs in rats, we strived to establish a bio-
photon imaging technology that can be applied to the early diagnosis and disease process evaluation in
patients with arthritis.
2. MATERIAL AND METHODS
2.1. Rat Model of RA
Male Wistar rat (3 - 4 weeks, 62 ± 5 g) were purchased from Hubei Provincial Laboratory Animal
Public Service Center (Wuhan, China) and housed under standard conditions (12-h light/dark cycle, room
temperature 18˚C - 25˚C, 40% - 50% humidity) with access to food and water ad libitum. The protocols
were approved by the committee on the Ethics of Animal Experiments of South-Central university for Na-
tionalities. Ten rats were divided into two groups (5 in each group and numbered). Complete Freunds
adjuvant (CFA) was injected subcutaneously in the plantar part of the right hind paw of one group and the
left hind paw of the other group, and the contralateral hind paw was set as the control. The dose of the first
injection was 0.25 ml/100 g and the control was injected with the same dose of normal saline. The changes
in diet and activity and the state of lower limb joints of rats in two groups were observed every day.
2.2. Pain Sensitivity Test
Heat sensitivity of the rat hind paw was determined using a Plantar Test Apparatus (ZH-200, Zheng-
huabiologic, China). Rat was placed in a plastic box with a glass floor. After a 3-min habituation period,
the plantar surface of hind paw was exposed to a beam of radiant heat through the glass floor. The baseline
latencies were averaged over 4 trials, separated by a 10-min interval. The test was carried out once a day. A
total of 17 tests were conducted, including the first one before CFA injection.
2.3. Biophoton Imaging
The rats hind paw was imaged with UBIS according to the previous report [17]. The rat was fixed on
a device and then put into the dark box of UBIS. The biophoton imaging was carried out with an EM-
CCD. The specific imaging parameters were as follows: 1) EM-CCD cooling temperature is 95˚C; 2)
1200× gain; 3) the exposure time is 900 s for each frame image; 4) the time course of imaging is 1.5 h.
2.4. Image Processing and Data Analysis
A series of images were processed using the previously reported methods [17], including the removal
of bright spots caused by the cosmic radiation, and the extraction of gray values from the region of inter-
esting (ROI), such as the plantar joint of the hind limb of rat and background area.
Relative gray values (RGVs) = the gray values of the region of interesting (ROI)
the gray values of background area.
2.5. Statistics
Statistical analyses were performed using Microsoft Excel and two-tailed paired T-test was used to
compare the differences between the treated group and control group. Probability values of
p
< 0.05 were
regarded as significant.
https://doi.org/10.4236/ns.2021.1310037 453 Natural Science
3. RESULTS
3.1. CFA-Induced Rat Model of RA
During the experiment, except that the experimental group had different degrees of claudication in
the lower limbs after injection of CFA for a period of time, no obvious behavioral abnormalities and
weight changes were found in two groups. Seven days after injection of CFA, the plantar joint of rat hind
limb presented swelling (Figure 1(A)), whereas the hind paws in controls had no such phenomenon
(Figure 1(B)).
3.2. Changes in Nociceptive Threshold
As shown in Figure 2, there was no difference between the plantar part of the right and left hind paw
in two groups before CFA injection. Heat hyperalgesia was found in the CFA injection hind paw lasting
from the second day after CFA injection to the 16th days. The most obvious pain sensitive reaction was at
the 7th day after injection. A slow recovery process occurred until 12th day, and then there was a period of
increased sensitivity.
The dynamic changes in the reaction time of the hind paw both in RA and control groups.
Figure 1. The changes in CFA-induced rat model of RA. (A) A representative
regular image of the RA rat’s hind paw, showing obvious swelling 7 days after
injection of CFA; (B) A representative image of the normal rat’s hind paw.
Figure 2. The hind paw reaction time induced by thermal stimulus.
https://doi.org/10.4236/ns.2021.1310037 454 Natural Science
3.3. Biophoton Emissions Were Higher in RA
A swollen hind paw picture (Figure 3(A)) in a CFA treated rat corresponds to an image of biophoton
imaging (Figure 3(B)). The regular picture and the image of biophoton imaging in a control rat hind paw
are shown in Figure 3(C) and Figure 3(D), respectively. There was a significant decline of biophoton
emission during the first 30 min, then a slightly decay was observed within the next 60 min. The biopho-
ton emissions were significantly higher in the CFA treated paws than that in the controls at the different
time points (Figure 3(E), Table 1, n = 7).
Table 1. Comparison of relative gray values between the CFA treated and control hind paws at the
different time points.
Time (min)
Treated
Control
P
value
15
104.8 ± 37.90
84.54 ± 23.78
0.29
30
55.71 ± 14.39
34.62 ± 9.86
0.012**
45
42.33 ± 7.52
26.11 ± 6.64
0.0019***
60
36.56 ± 3.87
20.18 ± 4.32
0.000016***
75
29.85 ± 4.94
17.45 ± 5.07
0.0011**
90
29.67 ± 5.32
15.13 ± 6.79
0.0014**
Asterisks indicate a significant difference, n = 7, **
p
< 0.01; ***
p
< 0.001.
Figure 3. Biophoton imaging of hind paw in rats treated with CFA and saline. (A) A representative
regular image of rat hind paw treated with CFA; (B) The corresponding image of biophoton imaging
to A, showing obvious biophoton emissions in the region of hind paw (bright area). (C) A repre-
sentative regular image of control rat hind paw. (D) The corresponding image of biophoton imaging
to C, showing relative weak biophoton emissions in the region of hind paw (bright area) as com-
pared to B. (E) The dynamic changes in biophoton emissions from the hind legs both the treated and
control groups at the different time points. Exposure time = 900 s for each frame image. RGVs: Rel-
ative gray values, n = 7, **:
p
< 0.01, ***:
p
< 0.001.
https://doi.org/10.4236/ns.2021.1310037 455 Natural Science
4. DISCUSSION
Rheumatoid arthritis is the most commonly diagnosed systemic inflammatory arthritis, and it carries
substantial burden for both the individual and society. For individual, they may suffer from some muscu-
loskeletal deficits, cumulative comorbid risk and quality of life [18]. For society, not only it will cost medi-
cal resources, but also, as a consequence of functional disability, it may directly relate to the reduced work
capacity and decreased societal participation [19]. However, up to now there are no reliable diagnostic cri-
teria for rheumatoid arthritis [20], and it needs to combine many parameters including the clinical symp-
toms and the various experimental tests and imaging techniques such as radiological imaging and blood
and serology assessment, etc. [21, 22], therefore, the early diagnosis and evaluation of the disease process is
usually difficult. In this study, we found that the biophoton emission in the hind paws of RA rats was sig-
nificantly higher than that of the control group, indicating that this novel biophoton imaging technique
may give a new way to solve this problem.
Many studies have shown that nonspecific inflammation plays an important role in the development
of RA, accompanied by increased formation of free radicals [23]. The generation of free radicals is also an
important source of biophotons [10]; therefore, by monitoring the degree of joint biophoton emission, it is
not only possible to realize the early diagnosis of RA, but also to monitor the development of disease. In
addition, this technique also provides a new method for further using RA animal model to study its pa-
thogenesis and develop new drugs.
ACKNOWLEDGEMENTS
This work was supported by the research funds of South-Central University for Nationalities
(XTZ15014 and CZP 18008).
CONFLICTS OF INTEREST
The authors declare no conflicts of interest regarding the publication of this paper.
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