ArticlePDF AvailableLiterature Review

Cinnamon as a Useful Preventive Substance for the Care of Human and Plant Health


Abstract and Figures

Cinnamon is widely used as a food spice, but due to its antibacterial and pharmacological properties, it can also be used in processing, medicine and agriculture. The word “Cinnamon” can refer to the plant, processed material, or an extract. It is sometimes used as a substance, and sometimes used as a mixture or as compounds or a group. This article reviews research into the effectiveness of various forms of cinnamon for the control of plant diseases and pests in crops and during storage of fruit and vegetables. Cinnamon acts on pests mainly as a repellent, although in higher doses it has a biocidal effect and prevents egg-laying. Cinnamon and its compounds effectively hinder bacterial and fungal growth, and the phytotoxic effects of cinnamon make it a possible herbicide. This article presents the wide practical use of cinnamon for various purposes, mainly in agriculture. Cinnamon is a candidate for approval as a basic substance with protective potential. In particular, it can be used in organic farming as a promising alternative to chemical pesticides for use in plant protection, especially in preventive treatments. The use of natural products is in line with the restriction of the use of chemical pesticides and the principles of the EU’s Green Deal.
Content may be subject to copyright.
Molecules 2021, 26, 5299.
Cinnamon as a Useful Preventive Substance for the Care
of Human and Plant Health
Jolanta Kowalska 1,*, Józef Tyburski 2, Kinga Matysiak 3, Magdalena Jakubowska 4, Joanna Łukaszyk 1
and Joanna Krzymińska 1
1 Department of Organic Agriculture and Environmental Protection, Institute of Plant ProtectionNational
Research Institute, Władysława Węgorka 20, 60-318 Poznań, Poland; (J.Ł.); (J.K.)
2 Department of Agroecosystems and Horticulture, University of Warmia and Mazury in Olsztyn,
Michała Oczapowskiego 2, 10-719 Olsztyn, Poland;
3 Department of Weed Science and Plant Protection Techniques, Institute of Plant ProtectionNational
Research Institute, Władysława Węgorka 20, 60-318 Poznań, Poland;
4 Department of Monitoring and Signalling of Agrophages, Institute of Plant Protection National Research
Institute, Władysława Węgorka 20, 60-318 Poznań, Poland;
* Correspondence:
Abstract: Cinnamon is widely used as a food spice, but due to its antibacterial and pharmacological
properties, it can also be used in processing, medicine and agriculture. The word “Cinnamoncan
refer to the plant, processed material, or an extract. It is sometimes used as a substance, and some-
times used as a mixture or as compounds or a group. This article reviews research into the effec-
tiveness of various forms of cinnamon for the control of plant diseases and pests in crops and
during storage of fruit and vegetables. Cinnamon acts on pests mainly as a repellent, although in
higher doses it has a biocidal effect and prevents egg-laying. Cinnamon and its compounds effec-
tively hinder bacterial and fungal growth, and the phytotoxic effects of cinnamon make it a possi-
ble herbicide. This article presents the wide practical use of cinnamon for various purposes, mainly
in agriculture. Cinnamon is a candidate for approval as a basic substance with protective potential.
In particular, it can be used in organic farming as a promising alternative to chemical pesticides for
use in plant protection, especially in preventive treatments. The use of natural products is in line
with the restriction of the use of chemical pesticides and the principles of the EUs Green Deal.
Keywords: basic substance; human health; insecticidal activity; microbial activity; plant diseases
1. Introduction
Cinnamon (Cinnamomum zeylanicum L. and Cinnamon cassia L.), a species of the
Lauraceae family, is an evergreen tree of the tropics, which is widely used in medicine,
and offers a rich variety of applications worldwide. The word was adopted by English
towards the end of 14th century as a loanword from the old French “cinnamone”, which
in term came from Latin via the Greek-Phoenician word “kinnamomon” and supposed
to be from Semitic cf. Hebrew “qinamon”. The first appearance of the word in print dates
to 1430, in John Lydgate Bochas“Fall of Princes” [1].
Cinnamon contains manganese, iron, dietary fibre, and calcium. It has derivatives,
such as cinnamaldehyde (CNAD), cinnamic acid, cinnamate, and many other ingredi-
ents, such as polyphenols and antioxidants, with anti-inflammatory, antidiabetic, anti-
microbial, and anticancer properties. Several reports have shown numerous properties of
cinnamon in the form of bark and bark powder. Essential oils and phenolic compounds
in cinnamon contribute positively to human health. Studies have recently shown the
positive influence of cinnamon in the treatment of Alzheimer’s disease, diabetes, arthri-
tis, and arteriosclerosis [2].
Kowalska, J.; Tyburski, J.;
Matysiak, K.; Jakubowska, M.;
zyk, J.; Krzymińska, J.
Cinnamon as a Useful Preventive
Substance for
the Care of Human
and Plant Health.
Molecules 2021, 26,
Academic Editor
s: Gianfranco
and Silvia Bautista-Baños
Received: 28 June 2021
Accepted: 24 August 2021
31 August 2021
Publisher’s Note:
MDPI stays
neutral with regard to jurisdictional
claims in published maps and
institutional affiliations.
© 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
This article is an open access article
distributed under the terms and
conditions of the Creative Commons
Attribution (CC BY) license
Molecules 2021, 26, 5299 2 of 14
Wang et al. [3] reported other major compounds in cinnamon: coumarin, cinnamyl
alcohol, cinnamaldehyde, cinnamic acid, eugenol, and cinnamyl acetate [3]. Tung et al.
[4] have also reported the presence of a wide range of essential oils in cinnamon, such as
trans-cinnamaldehyde, cinnamyl acetate, eugenol, L-borneol, caryophyllene oxide,
b-caryophyllene, L-bornyl acetate, E-nerolidol, α-cubebene, α-terpineol, terpinolene, and
α-thujene. Cinnamon consists of a variety of resinous compounds (Table 1).
Table 1. Various chemical constituents of the cinnamon plant [5].
Part of the Plant
Cinnamaldehyde: 1.00 to 5.00%
Eugenol: 70.00 to 95.00%
Cinnamaldehyde: 65.00 to 80.00%
Eugenol: 5.00 to 10.00%
Root bark
Camphor: 60.00%
Fruit trans-Cinnamyl acetate (42.00 to 54.00%)
nd caryophyllene
(9.00 to 14.00%)
Buds (C. zeylanicum)
Terpene hydrocarbons: 78.00%
α-Bergamotene: 27.38%
α-Copaene: 23.05%
Oxygenated terpenoids: 9.00%
Flowers (C. zeylanicum)
(E)-Cinnamyl acetate: 41.98%
trans-α-Bergamotene: 7.97%
Caryophyllene oxide: 7.20%
According to other sources, ground cinnamon contains carbohydrates, fibre, mois-
ture, protein, fat, and ash. It also contains vitamins A, B, C, E, K, and lipids. The compo-
sition is different depending on the geographical origin and the processing methods [6,7].
As a plant, cinnamon contains many substances and substance groups. Among
these, there are essential oils, diterpenes, catechins, proanthocyanidins, tanning agents,
colouring agents, phenolic carboxylic acids, lignans, and mucins. Cinnamon's essential
oils mainly have antifungal and antibacterial properties and, similarly to cinnamon bark
extract, are characterized by antioxidant activity [8]. Moreover, essential oils have anti-
inflammatory, antidiabetic, antitumor, antimutagenic, and memory-enhancing proper-
ties. Cinnamaldehyde and eugenol are active components against Gram-positive and
Gram-negative bacteria [9].
Sharifi-Rad et al. 2021 [10] showed that the bioactive compounds of Cinnamomum
species possess antimicrobial, antidiabetic, antioxidant, anti-inflammatory, anticancer,
and neuroprotective effects.
Incomplete knowledge about the safe consumption of higher doses of cinnamon on
a daily basis makes it necessary to assess the occurrence of this risk, and therefore, the
long-term use of a high amount of cinnamon should be monitored. The tolerable daily
intake for coumarin (0.1 mg/1 kg body weight) can be regarded as safe in terms of daily
cinnamon intake without the risk of adverse effects [11]. According to the scientific data
currently available, a risk assessment must be focused on the problematic ingredients of
cinnamon extract, especially on coumarin, trans-cinnamaldehyde, safrol, and styrene,
which are toxic.
Molecules 2021, 26, 5299 3 of 14
Cinnamon bark is obtained twice a year, closely following each of the rainy seasons,
when the air humidity facilitates bark harvesting. The first harvest is done when the trees
are three years old, a year after pruning. The side stems that are about three-years-old are
cut off, and the bark is pulled off. Cinnamon bark is gained only from stems that are
between 1.2 and 5 cm in diameter.
Cinnamon is often ground to a powder before sale. The powder should be packed in
moisture-proof wrapping (polypropylene bags) to keep the flavour. Polyethylene pack-
aging is not advisable, as the flavour components diffuse through it [12].
2. Culinary and Medicinal Use
Cinnamon bark is commonly used as a spice. It is principally used in cooking as a
condiment and flavouring agent. It is used in the production of chocolate, especially in
Mexico, which is the biggest importer of true cinnamon (C. zeylanicum L.). It is also added
to desserts, such as apple pie, donuts, and cinnamon buns, as well as spicy candies, tea,
hot cocoa, and liqueurs. True cinnamon and not cassia (C. cassia L.) is better for use in
sweet dishes. In the Middle East, it is often used in savoury dishes of chicken and lamb.
In the USA, cinnamon is often used as an additive to flavour cereals, bread-based dishes,
and fruit, especially apples; and a cinnamonsugar mixture is on sale in grocery stores.
Another use of cinnamon is in pickling.
Cinnamon bark is one of the rare spices that can be consumed directly; cinnamon
powder has long been an important spice in Persian cuisine, added to various thick
soups, drinks, and sweets. It is often used as a mixture with rosewater or other spices to
make a cinnamon-based curry for stews or just sprinkled on sweet desserts [13].
2.1. Effects in Humans
Cinnamaldehyde (CNAD) lowers inflammatory reactions, oxidative stress, and
apoptosis of the liver in Salmonella typhimurium-infected mice. Supplementation of
CNAD might be a good preventive method to alleviate the liver damage caused by Sal-
monella typhimurium infection in humans and animals [14]. Moreover, cinnamon bark
essential oils (EOs) have been shown to cause oxidative stress to Klebsiella pneumoniae,
resulting in the loss of cell viability [15]. Both oregano and cinnamon bark EOs have
strong antibacterial properties. Aljaafari et al. [16] have shown that the antimicrobial
properties of essential oils (EOs) are based on the mode of action in relation to membrane
disruption, efflux inhibition, the increase in membrane permeability, and the decrease in
intracellular ATP. These essential oil compounds can be used as potential agents against
bacteria, fungi, and viruses. In the future, the integration of EOs uses can lead to many
clinical applications.
In medicine, the essential oils in cinnamon behave like other volatile oils. It has also
been used in the treatment of digestive system problems and colds. The essential oils in
cinnamon also have antimicrobial properties and are used as a preservative in some
foods. Cinnamon has been reported to have remarkable pharmacological effects in the
treatment of diabetes type 2 resistant to both mellitus and insulin; however, the plant
material used in the study was mainly from cassia and only some of the plant material
was from C. zeylanicum. Cinnamon has traditionally been used for toothache and to fight
bad breath, and its regular use is thought to cure the common cold and support digestion
[17]. It is noted that regular drinking of C. zeylanicum tea made from the bark could be
helpful in oxidative-stress-related illness in humans, since it has considerable antioxidant
potential. Cinnamon may also act as an aphrodisiac. One teaspoon of cinnamon has as
many antioxidants as a cup of pomegranate juice and half a cup of blueberries [17].
Nanocapsules with cinnamon-thyme-ginger composite essential oils prepared with
chitosan as the wall via ionic gellification reaction were tested in medicine and revealed
durable antibacterial activity against Escherichia coli, Bacillus subtilis, and Staphylococcus
aureus. Composite essential oil nanocapsules CEO-NPs can be applied as a strong
long-lasting natural preservative [18].
Molecules 2021, 26, 5299 4 of 14
2.2. Adverse Effects Reported in Humans
Scientific research has confirmed the effectiveness of cinnamon in fighting microbes,
viruses, fungi, oxidants, tumours, and hypertension. It also has antidiabetic, gas-
tro-protective, and immune modulatory potential [17]. However, the popular use of
cinnamon has also resulted in several reports of side effects from its short- and long-term
use. The most common negative effects were disorders of the stomach and bowels, as
well as allergic reactions, which were self-controlling in most cases. Although cinnamon
is safe as a spice and/or flavour, prolonged and enlarged use may be a health risk, and
hence, in medicinal uses, it should be clinically monitored [19].
Cinnamon coats and dries the mouth and throat, leading to coughing, gagging,
vomiting, and the inhalation of cinnamon, causing throat irritation, breathing difficulties,
and a risk of pneumonia or lung collapse [20,21]. Cinnamon contact stomatitis (CCS) is
also a sporadic reaction to the consumption of foods containing artificial cinnamon fla-
vour. Physicians who treat patients with oral conditions ought to be aware of CCS to
correctly diagnose and manage this condition [22]. Contact stomatitis, which is related to
the use of cinnamon flavourings, is rather rare. The symptoms, as well as the histopath-
ologic features of this disease are non-specific. They may be similar to other inflamma-
tory illnesses of the oral mucosa, which causes problems in diagnosis. Patients develop
white and erythematous spots of rapid occurrence, with an associated sensation of
burning. High levels of coumarin and cinnamaldehyde might be associated with mouth
sores [23]. High levels of coumarin and cinnamaldehyde might be also correlated to liver
damage and low blood sugar [24]; such high levels may increase the risk of cancer,
breathing problems, and interaction with certain medications [11].
Oral lesions caused by a reaction to cinnamon flavouring agents are rather uncom-
mon and are probably unrecognized by many physicians. Most patients feel a “burning
sensation”, which is the primary symptom. Clinically, lesions present as erythematous
patches with different degrees of superimposed keratosis or ulceration. The lesions are
usually limited to the buccal mucosa and lateral border of the tongue. The responsible
agent was most frequently cinnamon-flavouring chewing gum, and symptoms usually
eased within 1 to 2 days after the last use of the product containing cinnamon [25].
3. Agricultural Purpose
3.1. Fungicidal Activity
Cinnamon oils and extracts possess antifungal properties against serious plant dis-
eases (Table 2). Wilson et al. [26] indicated that, out of 49 essential oils tested, cinnamon
leaf C. zeylanicum showed the strongest antifungal activity against Botrytis cinerea. Mon-
tes-Belmont and Carvajal [27] found that Aspergillus flavus was fully inhibited by C.
zeylanicum. In other studies, C. zeylanicum proved to be fungicidal towards pathogens
(Colletotrichum musae, Lasiodiplodia thebromae, and Fusarium proliferatum) isolated from
bananas [28]. Cinnamon also had an antifungal effect against Oidium murrayae [29] and
harnessed conidial germination of Colletotrichum gloesporioides [30]. In in vitro tests, it
proved to have a significant mycelial inhibition in corn rot Fusarium oxysporum f. sp.
gladioli [31] and to be very effective against the growth of Rhizoctonia solani [32]. Moreo-
ver, cinnamon has powerful antifungal activity towards early tomato blight (Alternaria
solani) [33]. Botrytis cinerea is a serious problem, especially in horticultural crops. The in-
vestigations of Wang et al. [34] demonstrated that cinnamon microemulsions possessed
high in vivo control properties against gray mould of pears, B. cinerea. The influence of C.
zeylanicum organic powder on the growth of B. cinerea and its effect on tomato plants
have also been assessed. Cinnamon bark powder and also its water suspensions and fil-
trates controlled B. cinerea; moreover, tomato plants sprayed with cinnamon developed
better than the control plants [35].
An in vitro study on extracts from the cinnamon bark of C. cassia and clove (Syzyg-
ium aromaticum L.) on the growth of B. cinerea colonies showed their antifungal activity,
Molecules 2021, 26, 5299 5 of 14
causing a slow growth of this pathogen and can be applied to control strawberry gray
mould. The clove extract consisted of 52.88% eugenol, and the cinnamon extract con-
sisted of 74.67% cinnamaldehyde. The efficacy of the extracts on detached strawberry
leaves showed that a 12 mL/L concentration of clove extract was effective in suppressing
grey mould infection. It is worth noting that the results indicate that the antifungal
properties of the clove extract were more effective (even applied in a lower concentra-
tion) than that of the cinnamon extract. Grey mould infection on detached strawberry
leaves was inhibited by the use of clove oil at the higher investigated concentration (12
mL/L) [36]. The cinnamon extract applied at a rate of 12 mL/L proved to be less effective
at inhibiting the spread of grey mould on strawberry leaves [37], and Allam et al. [38]
reported that a higher concentration of 20 mL/L of cinnamon entirely inhibited the my-
celial growth of B. cinerea in vitro.
Cinnamaldehyde has been used effectively as a bio-fungicide for controlling other
plant fungal diseases. For example, it showed good inhibitory activity against Colleto-
trichum lagenarium, a significant plant-pathogenic fungus leading to anthracnose of cu-
cumber. Other studies indicate that it markedly inhibited zoospore germination and the
rapid mycelial development of Phytophthora capsici, a pathogenic fungus causing phy-
tophthora blight of pepper [39]. Essential oil extracted from C. zeylanicum (CEO) leaves
was identified as having the active constituents eugenol and trans-cinnamaldehyde,
which had, respectively, minimal inhibitory concentrations (MICs) of 250 and 62.5 μg/mL
against Alternaria alternata, while, under the same conditions, the MICs for a commercial
fungicide and CEO were 1250 and 500 μg/mL, respectively [40].
Cinnamon essential oil proved to be more capable of limiting the incidence and
progress of fungal disease in commercial tangerine orchards than copper fungicide and
was effective at a similar level as a commercial plant activator. Both essential oil and
cinnamaldehyde (additional to the direct way of action, inhibiting fungal growth), posi-
tively influence the plant defense system, causing a significant rise in enzyme levels [40].
It was conferred that cinnamon oil has powerful antifungal activity against these four
species of fungi: Aspergillus niger, Penicillium notebookum, Mucora heimalis, and Fusarium
oxysporum. Cinnamon oils and cinnamon extracts have demonstrated good antifungal
properties against economically important plant diseases [41].
The effectiveness of essential oil from clove and cinnamon against fungi resulting in
the postharvest decay of grapes: A. niger, A. alternata, Colletotrichum gloeosporioides, L.
theobromae, Phomopsis viticola, and Rhizopus stolonifer has also been investigated. In the
study of Udomlak Sukatta [42] the antifungal activity of clove oil against all the
above-mentioned fungi showed minimal inhibitory concentrations (MICs) of: 200, 200,
400, 800, 200, and 200 mg/mL, respectively, whereas the MICs obtained from cinnamon
oil were 50, 100, 200, 200, 100, and 800 mg/mL, respectively. Investigation of the syner-
gistic effect of clove and cinnamon oil showed three optimum ratios: 3:7, 2:8, and 1:9 and
MICs for all fungi obtained from these ratios for the inhibition of the growth of six fungi
was 400 mg/mL. A study of the synergistic effect of clove and cinnamon oil indicated
three optimum proportions: 3:7, 2:8, and 1:9, and the MICs for all fungi obtained from
these ratios to inhibit the development of six fungi was 400 mg/ mL [42].
A further study determined if essential oils can be applied as a contact fungicide
seed treatment for organic maize. The sowing date for organic maize (Zea mays L.) is de-
layed to avoid the coldness and wetness of spring soils. Conventional farmers can apply
chemical fungicide seed treatments to protect the emerging seedling but almost no or-
ganic fungicides are on the market. Eighteen plant essential oils were studied for their
fungicidal activities against three common maize pathogens: Penicillium spp., Fusarium
spp., and Pythium spp. Five oils fully controlled all three pathogens in vitro. These oils
were cinnamon, clove, oregano, savory, and thyme. The MIC for all pathogens was 800
μL/L, and no phytotoxicity was detected in the germination test at doses up to 16,000
μL/L (MIC x 20). The field emergence of inbred and hybrid seeds treated with the essen-
Molecules 2021, 26, 5299 6 of 14
tial oil were considerably decreased compared to seed treated with the commercial,
conventional fungicides and one organic fungicide [43].
Fusarium wilt caused by F. oxysporum f. sp. lycopersici is the most serious disease of
the tomato. In the study of Prashant et al. [44], seven plant extracts were screened against
F. oxysporum. Among these, the antifungal properties of S. aromaticum and C. zeylanicum
extracts were comparable to the efficiency of chemical fungicides. Methanol (MeOH) ex-
tract of S. aromaticum demonstrated the widest range of inhibition as compared to other
extracts assessed, including those from C. zeylanicum. Solvent extracts of cinnamon and
clove proved to be 100% inhibitory against F. oxysporum spores at 5 and 10 mL/L rates
Another pathogen, Fusarium verticillioides, is a filamentous fungus and a commonly
occurring pathogen with the ability to infect and destruct economically important crops
by producing fumonisin mycotoxins. Xing et al. [45] carried out a study to assess the in-
hibitory properties of cinnamon and clove, eucalyptus, anise, spearmint, and camphor
oils on F. verticillioides. Cinnamon oil proved to have the strongest inhibition properties.
The antifungal potential of cinnamon oil was investigated with a special focus on its
mechanism of inhibition of F. verticillioides development at the morphological and ul-
tra-structural planes. For F. verticiliides, the minimal inhibitory concentrations (MICs) of
cinnamon oil (85% cinnamaldehyde), natural cinnamaldehyde (95%), and synthetic cin-
namaldehyde (99%) were 60, 50, and 45 mL/L, respectively. The same authors [45] used
scanning electron microscopy and its transmission version of F. verticillioides in the
presence of MIC of cinnamaldehyde and showed irreversible deleterious morphological
and ultra-structural changes, such as a lack of cytoplasmic content, depravation of integ-
rity and rigidity of the cell wall, plasma membrane disintegration, mitochondrial de-
struction, and the folding of the cell. These alterations caused by cinnamaldehyde may
result from its interference with the enzymatic reactions of cell wall synthesis, thus neg-
atively influencing the morphogenesis and development of the fungus. These outcomes
further emphasized the toxicity of cinnamon oil towards F. verticillioides attacking grains.
It shows that cinnamon oil can be safely applied as an alternative to chemical fungicides
during grain storage. The cinnamon oil concentration proved to be effective on the de-
velopment of F. verticillioides with fumigation. The inhibitory effect of cinnamon oil rose
proportionally to its concentration and proportionally to treatment duration. An increase
in cinnamon oil concentration resulted in a delay in conidia germination and showed
different inhibitory reactions. At a rate of 40 mL per Petri dish after 6 days of incubation,
the cinnamon oil fully inhibited F. verticillioides mycelial growth. After 20 days of incu-
bation, visible development of F. verticillioides did not take place. These results indicate
the fungistatic properties of cinnamon oil at lower ratios and fungicidal properties at
higher ratios [45]. The ability of cinnamon, clove, lemongrass, oregano, and palmarosa
essential oils to prevent the growth of fumonisin B1 (FB1) production by F. verticillioides
at different water activities (0.95 and 0.995 aw) and temperatures (20 and 30 °C) in irra-
diated maize grain was also evaluated by Velluti et al. [46]. All of the essential oils inhib-
ited growth of F. verticillioides isolates under all conditions tested, but FB1 production
was only inhibited at 30 °C and 0.995 aw.
The antifungal properties of cinnamon against other pathogens have been shown by
other researchers [47]. The in vitro efficacy of cinnamon essential oil was tested at appli-
cation rates of 100, 250, 500, 1000, and 2000 μL/L for controlling fruit rot. The mycelium
growth of Colletotrichum gloeosporioides sp., Fusarium solani, and Phytophthora palmivora
was inhibited at application rates of 1000 μL/L [47].
The antifungal effect of cinnamon oil has been studied with special reference to its
mechanism of inhibition on F. verticillioides growth at the morphological and ul-
tra-structural levels. The MICs of cinnamon oil (85% cinnamaldehyde), natural cin-
namaldehyde (95%), and synthetic cinnamaldehyde (99%) were 60, 50, and 45 μL/L, re-
spectively. The antifungal activity of cinnamon oil was proportional to its cinnamalde-
hyde concentration [45].
Molecules 2021, 26, 5299 7 of 14
A significant antifungal effect was observed with the essential oil of C. zeylanicum on
mycelial growth using bioassays of Fusarium oxysporum f. sp. gladioli at 100, 150, 200, 250,
and 300 ppm [31]. The important antifungal potential of cinnamon oil (both in vitro and
in vivo) in proportion to its concentration towards various Fusarium species was con-
firmed. In in vitro studies by Horváth et al. [48], the cinnamon oil effectively controlled
mycelial development of Fusarium head blight of winter wheat. Jiang et al. [49] demon-
strated that C. cassia oil has a significant antifungal effect against S. sclerotiorum with a
minimum inhibitory concentration (MIC) of 256 μg/mL in agar and 64 μg/mL in air. In a
further study, trans-cinnamaldehyde exhibited the highest antifungal activity among the
three cinnamaldehydes tested. Al-Taisan et al. [50] noted that cinnamon, clove, and mint
oils completely inhibited in vivo mycelial growth of S. sclerotiorum at 10500 ppm con-
centrations. A soil application containing cinnamon oil significantly reduced the inci-
dence of disease caused by S. sclerotiorum, producing 75% plant survival compared to the
control [43]. Moraes et al. [51] investigated the inhibitory properties of cinnamon (C. cas-
sia) and citronella (Cymbopogon winterianus) essential oils in the in vitro control of Asper-
gilus sp. and S. sclerotiorum fungi. The effectiveness of cinnamon and other essential oils
and microelements against Sclerotinia sclerotiorum was shown in in vitro tests. Cinnamon
and citronella essential oils were used in doses of 0.2, 0.4, 0.8, and 1.6 mL/L. The dose of
1.6 mL/L of both oils fully inhibited the mycelial development of Aspergillus sp. and S.
sclerotiorum fungi.
The continuous spread and evolution in the development of natural plant protec-
tive means as alternatives to synthetic fungicides attracts attention today. Combrinck et
al. [52] evaluated the antifungal properties of eighteen essential oils and their impact on
the growth of five pathogens in vitro (Lasiodiplodia theobromae, Colletotrichum gloeospori-
oides, Alternaria citrii, B. cinerea, and Penicillium digitatum) isolated from mango, avocado,
citrus, grapes, and cactus pear. Most of the oils were chosen because of their commercial
availability and the content of a predominant compound. A visual inspection of the
fungal growth was conducted, and the lowest ratio where the fungal growth was fully
stopped was noted. Rich in eugenol (81.2%), cinnamon oil showed a strong fungicidal
effect [45]. In other research, the antifungal properties of cinnamon extract (CE) were
assessed on banana crown rot fungi. The antifungal activities of cinnamon extract, pep-
per extract (PE), and garlic extract (GE) were evaluated on banana crown rot fungi
(Colletotrichum musae, Fusarium spp., and L. theobromae) in vitro. The assay was conducted
with extracts of CE, PE, and GE with concentrations of 0, 0.1, 0.5, 1.0, 5.0, 10.0, and 0.75
g/L of carbendazim (CBZ) on potato dextrose agar at room temperature. CE completely
inhibited the conidial germination and mycelial growth of all three fungi at 5.0 g/L. PE
totally suppressed mycelial growth of all fungi at 5.0 g/L and conidial germination at 10.0
g/L except for Fusarium spp. GE had no significant effects, but low concentrations (0.1
and 0.5 g/L) enhanced germ tube elongation of the three fungi. Crown rot growth was
also evaluated during banana storage at 13 °C for 7 weeks. The disease development was
weakest (25%) on CE-treated fruit after inoculation and stronger when CE was applied
prior inoculation. CE had no negative effects on the quality of the fruit, but CE used to-
gether with hot water treatment led to unacceptable skin browning [53]. The aim of an-
other study was to assess the antifungal potential of crude extracts of cinnamon and
rosemary against three isolates of sclerotinia carrot rot both under in vitro and in vivo
conditions. The extracts were obtained by applying two different solvents with ethyl ac-
etate (EA) and ethanol. The results indicated that crude extracts of cinnamon can reduce
the mycelial development of one isolate at the volatile and contact phase by 35.4% and
78.2%, respectively. Although crude extracts of cinnamon and rosemary could lower the
severity of carrot rot during carrot storage in contrast to EA, an extract of cinnamon
(2 g/ L) was confirmed to have a significant effect against this disease [54].
Molecules 2021, 26, 5299 8 of 14
Table 2. Selected examples of fungicidal activity of cinnamon components for agricultural purposes.
Form of Cinnamon
Effective Dose
Fusarium oxysporium
Essential oil
100300 ppm
Botrytis cinerea
Extract C. cassia
20 mL/L
Fusarium verticillioides
Essential oil
Cinnamon oil with cinnamaldehyde
60 μL/L
4560 μL/L
Colletotrichum gloerpoides
Phytophthora palmivora
Fusarium solani
Essential oil 1000 μL/L [47]
Sclerotinia sclerotiorum
Essential oil
10500 ppm
Sclerotinia sclerotiorum
C. cassia oil
256 μg/mL of agar
Sclerotinia scleriotiorum
Aspergillus sp.
C. cassia oil 1.5 mL/L [51]
Lasidiploidia theobromae
Alternaria citrii
Essential oil 1000 μL/L [54]
Colletotrichum musae
Lasidiploidia theobromae
Cinnamon extract 5 g/L [55]
Sclerotinia sclerotiorum
ethyl acetate cinnamon extract
2 g/L
Mohammed et al. [55] studied the potential of E-cinnamaldehyde (EC) against S.
sclerotiorum on potatoes and also the induction of glutathione S-transferase genes. The
findings indicated that EC could inhibit the mycelial growth of S. sclerotiorum.
E-cinnamaldehyde decreased white mould on potatoes in greenhouse trials; additionally,
E-cinnamaldehyde considerably strengthened the activity of glutathione S-transferase
(GST)-like genes identified from the pathogen genome [55].
Another in vitro study indicated the antifungal activity of five plant extracts, in-
cluding C. zeylanicum, conducted with either cold distilled water (CDW) or boiling water
(BDW) on two pathogenic fungi, A. alternata and F. oxysporum. The results indicate that
plant extracts, especially those treated with CDW, revealed a powerful antifungal activity
with significant inhibition of the development of the two tested fungi [56].
3.2. Bactericidal Activity
Imad et al. 2016 [57] established that C. zeylanicum possesses remarkable antimicro-
bial activity, which is predominantly due to E-cinnamaldehyde. These findings indicate
that the methanolic extract of C. zeylanicum is antifungal and antibacterial. Natural bio-
cides with C. zeylanicum bark essential oil have major potential as antimicrobials; this
potential is reduced due to volatility and the fast decomposition of the essential oil. To
prevent this and to lengthen the efficacy of this biocide, cinnamaldehyde (CNAD) was
encapsulated into mesoporous silica nanoparticles (MSNPs) in order to handle the
problem. To eliminate seedborne diseases, CNAD-MSNPs were dressed in a sodium al-
ginate seed coating. This system was tried against Pseudomonas syringae pv. pisi, respon-
sible for pea bacterial blight. However, the concentration of CNAD in the alginate coating
was <0.0000034% (v/v); this was up to 90,000-fold lower than the concentrations of free
cinnamon oil reported earlier to control some bacterial diseases [58].
Molecules 2021, 26, 5299 9 of 14
3.3. Insecticidal Activity
Cinnamon has been suggested for use as a repellent against insects. Cinnamon oils
and their components, such as cinnamaldehyde, are insecticidal compounds that have
been used against a variety of insects [41] (Table 3).
Table 3. Insecticidal activity of cinnamon.
Insect Pest Cinnamon Substance/Form
Effective Dose for
Reference No.
Mosquito larvae
Aedes aegyptii
Cinnamon oil C. cassia 58.41 mg/L [59]
Thrips tabaci Essential oil C. zeylanicum
Trade product with
70% oil
Mechoris ursulus C. cassia bark extract
2.55 mg/paper
Plutella xylostella
Tetranychus urticae
Essential oil C. cassia 5.96 μg/cm2 [61]
Myzus persicae
Essential oil C. cassia
6.50 μg/cm
Sitophilus granarius
Essential oil
1000 μL/L
Acanthoscelides obtectus
Essential oil C. zeylanicum
46.8 μL/kg beans
Tribolium castaneum
Sitophilus zeamais
Rhyzopertha dominica
Essential oil 0.10.2% mixed
with flour [63]
Sitophilus oryzae
Callosobruchus chinensis
Extract of C. sieboldii root bark,C.
cassia extract and oil
0.7 mg/cm2 [64]
Cydalima perspectalis
Cinnamon oil as deterrent
1.5 mL in dispenser
Source: own study.
Cinnamon leaf oil proved to be very effective as a killing agent for mosquito larvae.
The compounds cinnamaldehyde, cinnamyl acetate, eugenol, and anethole, which are
ingredients of cinnamon leaf oil, were found to be the most effective against mosquito
larvae. The insecticidal and fumigant properties of C. cassia bark-derived materials
against the oak nut weevil (Mechoris ursulus) were examined using filter paper diffusion
and fumigation methods. In a test with the filter paper diffusion method,
trans-cinnamaldehyde showed 100 and 83.3% mortality at rates of 2.5 and 1.0 mg/filter
paper, respectively. At 2.5 mg/paper, strong insecticidal activity was produced from eu-
genol (90.0% mortality) and salicylaldehyde (88.9%), whereas trans-cinnamic acid had a
moderate activity (73.3%). At 5 mg/paper, weak insecticidal activity (50.0%) was pro-
duced from cinnamyl alcohol. In a fumigation study, the cinnamon bark-derived com-
ponents were considerably more effective in closed cups than in open ones. The results
indicated that the insecticidal activity of the tested compounds was attributable to fu-
migant action, but significant contact toxicity also occurred [59].
Cinnamon oil has been applied to control thrips on onions. The effects of orange and
cinnamon oil (from C. zeylanicum) on the presence and damage of Thrips tabaci on onions
was studied. The results (expressed as the number of thrips caught with sweeping nests)
confirm that both orange and cinnamon oil considerably reduced the number of adults
on onion plants [60]. To test the controlling potential against agricultural pests, such as
the diamondback moth (Plutella xylostella), green peach aphid (Myzus persicae), and two
spotted spider mite (Tetranychus urticae), the essential oils of Coriandrum sativum and C.
cassia obtained from steam distillation, hexane extraction, and supercritical extraction
were assessed. Using the contact bioassay, the LD50 values of C. cassia oils prepared by
steam distillation and hexane extraction methods were 5.96 and 4.64 μg/cm2, respectively,
against T. urticae adults, and the LD50 value of the essential oil by the supercritical ex-
traction method was 6.50 μg/cm2 against M. persicae adults. Finally, the research indicated
that C. cassia oils are a promising natural acaricide and insecticide against pests [61].
Molecules 2021, 26, 5299 10 of 14
In other studies the toxicity and deterrence of the terpenoid constituents of cinna-
mon oil have been evaluated. The toxic effects, repellent properties, and respiration rate
influenced by terpenoid components of cinnamon essential oil were assessed, as well
their influence on Sitophilus granarius L. The lethal concentrations (LC50 and LC90), repel-
lence in general, and behaviour repellence reaction of adult S. granarius in the presence of
six concentrations of respective essential oils, as well as terpenoids, were assessed. The
chemical constituents of the cinnamon oil were established, and its primary compounds
were eugenol (10.5%), trans-3-caren-2-ol (10.2%), benzyl benzoate (9.99%), caryophyllene
(9.34%), eugenyl acetate (7.71%), α-phellandrene (7.41%), and α-pinene (7.14%). The
toxicity of cinnamon essential oil against S. granaries was showed. Among the toxic ter-
penoid components, eugenol has the most powerful contact toxicity against S. granarius
in comparison to, in decreasing order, caryophyllene oxide, α-pinene, α-humulene, and
α-phellandrene. Cinnamon essential oils, as well as their terpenoid compounds, proved
toxic and had repellent properties against adult S. granarius and thus have preventive
and retarding properties for the development of resistance to insecticide [62].
In another study with storage pests, the bean weevil Acanthoscelides obtectus, which is
the cause of heavy post-harvest losses in the common bean, Phaseolus vulgaris, the essen-
tial oil of C. zeylanicum was tested for insecticidal (lethal toxicity, disturbances to repro-
duction, and persistence of action) and repellent activities. The study revealed toxicity at
LD50 46.8 μL/kg beans, steadily reduced the growth rate of A. obtectus in a dose-related
manner, and showed a similar loss of its insecticidal potential with time. Cinnamon oil
also repelled the bean weevil. The results showed that cinnamon essential oil is an effec-
tive tool for protecting stored beans against A. obtectus in small storage facilities. Cinna-
mon oil inhibited the reproduction ability of the flour beetle (Tribolium castaneum), the
maize weevil (Sitophilus zeamais), and the lesser grain borer (Rhyzopertha dominica) at a
rate of 0.10.2%, mixed with wheat or wheat flour [63].
The insecticidal properties towards adults of S. oryzae L. and Callosobruchus chinensis
were tested by Soon-Il Kim et al. [64]. In a test with a filter paper diffusion method at 3.5
mg/cm2, an extract from C. cassia bark and oil was used. An extract from C. sieboldii root
bark gave 100% mortality at two days after treatment. At 0.7 mg/cm2, extracts from C.
cassia, C. sieboldii, and cinnamon oil were highly effective against both species. In a fu-
migation test with S. oryzae adults, the oils were much more effective in closed containers
than in open ones, indicating that the insecticidal activity of the oils was attributable to
fumigant action. The authors concluded that these plant extracts and essential oils could
be helpful in controlling field populations of S. oryzae and C. chinensis. In another paper
on Cydalima perspectalis, the results point to cinnamon oil as a good deterrent with the
strongest oviposition-deterring effect [65].
3.4. Nematicidal Activity
The nematicidal activity of C. cassia and C. zeylanicum oils (bark and green leaf) and
their chemical compounds against adult Bursaphelenchus xylophilus was tested by a direct
contact bioassay. The LC50 values for cassia oils (0.0840.085 mg/mL) and for cinnamon
oils (0.0640.113 mg/mL) were toxic towards adult B. xylophilus. Of 45 tested compounds,
trans-cinnamaldehyde (0.061 mg/mL) was the most active nematicide, followed by ethyl
cinnamate, α-methyl-trans-cinnamaldehyde, methyl cinnamate, and allyl cinnamate
(0.1140.195 mg/mL). Potent nematicidal activity was also observed with
4-methoxycinnamonitrile, trans-4-methoxycinnamaldehyde,
trans-2-methoxy-cinnamaldehyde, ethyl α-cyanocinnamate, cinnamonitrile, and cin-
namyl bromide (0.2240.502 mg/mL). The tested compounds have been described as po-
tential nematicides to combat B. xylophilus, which causes pine wilt disease [66].
Molecules 2021, 26, 5299 11 of 14
4. Effect of Cinnamon Oil on Plant Growth
The impact of seed dressing with various concentrations of cinnamon oil and tea tree
oil on the field emergence and yield of parsley var. Berlinska and lettuce var. Ewelina is
presented in the literature [67]. Cinnamon oil lowered the lettuce and parsley field
emergence. The toxicity was the greatest at 15% concentration. Tee tree oil showed no
toxicity in 55 and 70% concentrations and increased the field emergence ability, particu-
larly in lettuce. A similar relation was established for the speed and spread of emergence.
A 15% concentration of cinnamon oil caused a decrease in the number of plants for both
species [67].
In laboratory and greenhouse investigations, the allelopathic effect of essential oils
extracted from aromatic plants (cinnamon, lavender, and peppermint) on the seed ger-
mination of mediterranean weed species (Amaranthus retroflexus L., Solanum nigrum L.,
Portulaca oleracea L., Chenopodium album L., Sinapis arvensis L., Lolium spp., and Vicia sativa
L.) was examined. Each essential oil was examined at four concentrations in controlled
conditions (germination chamber: 0.2, 0.6, 1.8, and 5.4 mg/L) and in a semi-controlled
condition (green house: 5.4, 21.6, 86.4, and 345.6 mg/L). In the controlled conditions, the
1.8 and 5.4 mg/L concentrations stopped seed germination. In the greenhouse
(semi-controlled conditions), the 345.6 mg/L concentration of cinnamon essential oil
stopped the seed germination of Amaranthus retroflexus L. The concentration of essentials
oils had a greater effect on weed susceptibility than the type of oil used. However, cin-
namon oil had drastic inhibitory effects on germination.
The possible use of essential oils as natural herbicides to control different weeds for
the sustainable cropping system has been discussed in relation to their low persistence
(due to biodegradability and easy catabolism in the environment); they have no toxicity
towards vertebrates, fishes, birds, and mammals and are of importance in plant protec-
tion [68].
In conclusion, cinnamon is a suitable candidate for approval as a basic substance
with protective potential. In particular, it can be used in organic farming as a promising
alternative to chemical pesticides for use in plant protection, especially in preventive
treatments. The use of natural products is in line with the restriction on the use of chem-
ical pesticides and the principles of the EUs Green Deal [69].
Author Contributions: J.K. (Jolanta Kowalska), J.Ł., and J.T. designed and drafted the work. K.M.
and J.K. (Joanna Krzymińska) contributed to the revision of the manuscript. M.J. substantively and
technically adapted the final version of the manuscript to the requirements of the editorial office.
All authors have read and agreed to the published version of the manuscript.
Funding: The funding body had no role in the design of the study; in the collection, analyses or
interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Data Availability Statement: Not applicable.
Acknowledgments: This study was a part of the statutory activity of the Institute of Plant Protec-
tionNational Research Institute in Poland. The project “Development of a strategy for the use of
microorganisms and natural products in organic farming” (No. EKO 01) was supported by the
Polish Ministry of Science and Higher Education. The work was prepared as part of the Eupresco
BasicS project.
Conflicts of Interest: The authors declare no conflict of interest.
1. Lankage, J. Cinnamon, tree that gave the name to the country and changed the course of history. J. Organ. Prof. Assoc. Sri Lanka
2013, 28, 4048.
2. Hariri, M.; Ghiasvand, R. Cinnamon and chronic diseases. Drug discovery from mother nature. Adv. Exp. Med. Biol. 2016, 929,
Molecules 2021, 26, 5299 12 of 14
3. Wang Y.-H.; Avula, B.; Nanayakkara, N.D.; Zhao, J.; Khan, I.A. Cassia cinnamon as a source of coumarin in cinnamon-flavored
food and food supplements in the United States. J. Agric. Food Chem. 2013, 61, 44704476, doi:10.1021/Jf4005862.
4. Tung, Y.-T.; Yen, P.-L.; Lin, C.-Y.; Chang, S.-T. Anti-inflammatory activities of essential oils and their constituents from
different provenances of indigenous cinnamon (Cinnamomum osmophloeum) leaves. Pharm. Biol. 2010, 48, 11301136.
5. Rao, P.V.; Gan, S.H. Cinnamon: A multifaceted medicinal plant. Evid. Based Complement. Altern. Med. 2014, 2014, 642942,
6. United States Department of Agriculture; Agricultural Research Service. National Nutrient Database for Standard Reference
Release Legacy April 2018. Basic Report 02010, Spices, Cinnamon, Ground. Available online:
https://Www.Ars.Usda.Gov/Arsuserfiles/80400525/Data/SR-Legacy/SR-Legacy_Doc.Pdf (accessed on 13 May 2021).
7. Senanayake, U.M.; Wijesekera, R.O.B. Chemistry of cinnamon and cassia. Cinnamon and cassia. The genus cinnamomum, 80
121. In Medicinal and Aromatic PlantsIndustrial Profiles; Ravindran, P.N., Ed.; CRC Press LLC: Boca Raton, FL, USA, 2004; p.
8. Perdones, A.; Vargas, M.; Atarés, L.; Chiralt, A. Physical, antioxidant and antimicrobial properties of chitosan-cinnamon leaf
oil films as affected by oleic acid. Food Hydrocoll. 2014, 36, 256264,
9. Sanla-Ead, N.; Jangchud, A.; Chonhenchob, V.; Suppakul, P. Antimicrobial activity of cinnamaldehyde and eugenol and their
activity after incorporation into cellulose-based packaging films. Packag. Technol. Sci. 2011, 25, 717,
10. Sharifi-Rad, J.; Dey, A.; Koirala, N.; Shaheen, S.; El Omari, N.; Salehi, B.; Goloshvili, T.; Cirone Silva, N.C.; Bouyahya, A.;
Vitalini, S.; et al. Cinnamomum species: Bridging phytochemistry knowledge, pharmacological properties and toxicological
safety for health benefits. Front Pharmacol. 2021, 12, 600139, doi:10.3389/fphar.2021.600139.
11. Abraham, K.; Wöhrlin, F.; Lindtner, O.; Heinemeyer, G.; Lampen, A. Toxicology and risk assessment of coumarin: Focus on
human data. Mol. Nutr. Food Res. 2010, 54, 228239, doi:10.1002/mnfr.200900281.
12. Azam-Ali, S. Practical Action. Cinnamon Processing. 2007. Available online: https://Core.Ac.Uk/Download/Pdf/48027458.Pdf
(accessed on 13 May 2021).
13. Cinnamon. Available online: https://Recipes.Fandom.Com/Wiki/Cinnamon (accessed on 12 May 2021).
14. Wang, R.; Li, S.; Jia, H.; Si, X.; Lei, Y.; Lyu, J.; Dai, Z.; Wu, Z. Protective effects of cinnamaldehyde on the inflammatory
response, oxidative stress, and apoptosis in liver of Salmonella typhimurium-challenged mice. Molecules 2021, 26, 2309,
https://Doi.Org/10.3390/Molecules26082309. (accessed on 23 June 2021).
15. Yang, S.-K.; Yusoff, K.; Ajat, M.; Thomas, W.; Abushelaibi, A.; Akseer, R.; Lim, S.-H.E.; Lai, K.-S. Disruption of kpc-producing
Klebsiella pneumoniae membrane via induction of oxidative stress by cinnamon bark (Cinnamomum verum ) essential oil. PLoS
ONE 2019, 14, E0214326.
16. Aljaafari, M.N.; Alali, A.O.; Baqais, L.; Alqubaisy, M.; Alali, M.; Molouki, A.; Ong-Abdullah, J.; Abushelaibi, A.; Lai, K.-S.; Lim,
S.-H.E. An overview of the potential therapeutic applications of essential oils. Molecules 2021, 26, 628,
17. Encyclopedia of Life EOL. Cinnamon. 2018. Available online: (accessed on 12 May 2021).
18. Jing, H.; Yudi, Z.; Zuobing, X.; Xuge, W. Preparation and properties of cinnamon-thyme-ginger composite essential oil
nanocapsules. Ind. Crops Prod. 2018, 122, 8592, doi:10.1016/J.Indcrop.2018.05.058.
19. Hajimonfarednejad, M.; Ostovar, M.; Raee, M.J.; Hashempurd, M.H.; Mayer, J.G.; Heydari, M. Cinnamon: A systematic review
of adverse events. Clin. Nutr. 2018, 38, 594602, https://Doi.Org/10.1016/J.Clnu.2018.03.013.
20. Cinnamon Challenge. Available online: Https://En.Wikipedia.Org/Wiki/Cinnamon_Challenge (accessed on 13 May 2021).
21. Grant-Alfieri, A.; Schaechter, J.; Lipshultz, S.E. Ingesting and aspirating dry cinnamon by children and adolescents: The
“cinnamon challenge”. Pediatrics 2013, 131, 833835, doi:10.1542/Peds.2012-3418.
22. Georgakopoulou, E.A. Cinnamon contact stomatitis. J. Dermatol. Case. Rep. 2010, 4, 2829, doi:10.3315/Jdcr.2010.1047.
23. Vivas, A.P.M.; Migliari, D.A. Cinnamon-induced oral mucosal contact reaction. Open Dent. J. 2015, 9, 257259.
24. Deng, R. A review of the hypoglycemic effects of five commonly used herbal food supplements. Recent Pat. Food Nutr. Agric.
2012, 4, 5060, doi:10.2174/2212798411204010050.
25. Allen, C.M.; Blozis, G.G. Oral mucosal reactions to cinnamon-flavored chewing gum. J Am. Dent. Assoc. 1998, 116, 664667,
26. Wilson, C.L.; Solar, J.M.; Ghaouth, A.E.; Wisniewski, M.E. Rapid evaluation of plant extracts And essential oils for antifungal
activity against Botrytis cinerea. Plant Dis. 1997, 81, 204210.
27. Montes-Belmont, R.; Carvajal, M. Control of Aspergillus Flavus in maize with plant essential oils and their components. J. Food.
Prot. 1998, 61, 616619.
28. Ranasinghe, L.; Jayawardena, B.; Abeywickrama, K. Fungicidal activity of essential oils of Cinnamomum zeylanicum (L.) and
Syzygium aromaticum (L.) Merr et L.M. Perry against crown rot and anthracnose pathogens isolated from banana. Lett. Appl.
Microbiol. 2002, 35, 208211.
29. Chu, Y.L.; Ho, W.C.; Ko, W.H. Effect of Chinese herb extracts on spore germination of Oidium murrayae and nature of inhibitory
substance from Chinese rhubarb. Plant Dis. 2006, 90, 858861.
30. Barrera-Necha, L.L.; Bautista-Bańos, S.; Flores-Moctezuma, H.E.; Rojas-Estudillo, A. Efficacy of essential oils on the conidial
germination, growth of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc and control of postharvest diseases in papaya
(Carica papaya L.). Plant Pathol. J. 2008, 7, 174178.
Molecules 2021, 26, 5299 13 of 14
31. Barrera-Necha, L.L.; Garduno-Pizana, C.; Garcia-Barrera, L.J. In Vitro antifungal activity of essential oils and their compounds
on mycelial growth of Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen. Plant Pathol. J. 2009, 8, 1721.
32. Nguyen, V.-N.; Seo, D.-J.; Park, R.-D.; Jung, W.-J. Antimycotic activities of cinnamon-derived compounds against Rhizoctonia
solani In Vitro. BioControl 2009, 54, 697707.
33. Yeole, G.J.; Teli, N.P.; Kotkar, H.M.; Mendki, P.S. Cinnamomum zeylanicum extracts and their formulations control early blight of
tomato. J. Biopestic. 2014, 7, 110.
34. Wang, Y.; Zhao, R.; Yu, L.; Zhang, Y.; He, Y.; Yao, J. Evaluation of cinnamon essential oil microemulsion and its vapor phase for
controlling postharvest gray mold of pears (Pyrus pyrifolia). J. Sci. Food Agric. 2014, 94, 10001004.
35. Kowalska, J.; Tyburski, J.; Krzymińska, J.; Jakubowska, M. Cinnamon powder: An In Vitro and In Vivo evaluation of antifungal
and plant growth promoting activity. Eur. J. Plant Pathol. 2020, 156, 237243, doi:10.1007/S10658-019-01882-0.
36. Oliveira, M.S.; Costa, W.A.; Pereira, D.S.; Botelho, J.R.S.; Menezes, T.O.A.; Andrade, E.H.A.; Silva, S.H.M.; Filho, A.P.S.S.;
Carvalho Junior, R.N. Chemical composition and phytotoxic activity of clove (Syzygium aromaticum) essential oil obtained with
supercritical CO2. J. Supercrit. Fluids 2016, 118,185193, doi:10.1016/j.supflu.2016.08.010.
37. Sernaite, L.; Rasiukevičiūtė, N.; Valiuškaitė, A. The extracts of cinnamon and cloves as potential biofungicides against
strowbery grey mould. Plants 2020, 9, 613, doi:10.3390/Plants9050613.
38. Allam, S.A.; Elkot, G.A.; Elzaawely, A.A.; El-Zahaby, H.M. Potential control of postharvest gray mold of pomegranate fruits
caused by Botrytis cinerea. Environ. Biodivers. Soil Secur. 2017, 1, 145156, doi:10.21608/JENVBS.2017.1822.1011.
39. Yan-Feng, X.; Meng, Z.; Zhong-Qiang, Q.; You-Qin, L.; Zhiqi, S.; Jian, C. Cinnemaldehyde promotes root branching by
regulating endogenous hydrogen sulfide. J. Sci. Food Agric. 2015, 96, 909914, doi:10.1002/Jsfa.7164.
40. Perina, F.J.; Lage De andrade, C.C.; Intra Moreira, S.; Nery, E.M.; Ogoshi, C.; Alves, E. Cinnamomun zeylanicum oil and
trans-cinnamaldehyde against alternaria brown spot in tangerine: Direct effects and induced resistance. Phytoparasitica 2019,
47, 575589, doi:10.1007/S12600-019-00754-X.
41. Haddi, K.; Faroni, L.R.A.; Oliveira, E.E. Cinnamon oil. In Green Pesticides Handbook. Essential Oils for Pest Control; Nollet,
L.M.L., Rathore, H.S., Eds.; CRC Press: New York, NY, USA, 2017; Chapter 7, p. 570, doi:10.1201/9781315153131.
42. Udomlak, S.; Vichai, H.; Walairut, C.; Panuwat, S. Antifungal activity of cinnamon oil and their synergistic against postharvest
decay fungi of grape In Vitro. Kasetsart J. Nat. Sci. 2008, 42, 169174.
43. Christian, E.J. Plant Extracted Essential Oils as a Contact Fungicide Seed Treatment for Organic Corn. Master’s Thesis, Iowa
State University, Ames, IA, USA, 2007, doi:10.31274/Rtd-180813-15735.
44. Yeole, G.J.; Kotkar, H.M.; Teli, N.P.; Mendki, P.S. Herbal fungicide to control fusarium wilt in tomato plants. Biopestic. Int.
2016, 12, 2535.
45. Xing, F.; Huijuan, H.; Selvaraj, J.N.; Yueju, Z.; Lu, Z.; Xiao, L.; Yang, L. Growth inhibition and morphological alterations of
Fusarium verticillioides by cinnamon oil and cinnamaldehyde. Food Control 2014, 46, 343350, doi:10.1016/J.Foodcont.2014.04.037.
46. Velluti, A.; Sanchis, V.; Ramos, A.J.; Marín, S. Effect of essential oils of cinnamon, clove, lemon grass, oregano and palmarosa
on growth of and fumonisin B1 production by Fusarium verticillioides in maize. J. Sci. Food Agric. 2004, 84, 11411146, doi:
47. Sarkhosh, A.; Schaffer, B.; Vargas, A.I.; Palmateer, A.J.; Lopez, P.; Soleymani, A. In Vitro evaluation of eight plant essential oils
for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya. Plant
Prot. Sci. 2018, 54, 153162, doi:10.17221/49/2017-Pps.
48. Horváth, A.; Kovács, B.; Nagy, G. Application of mint and cinamon against fusarium disease of winter wheat. Episteme
Czasopismo Naukowo-Kulturalne 2013, 18, 297304.
49. Jiang, Z.; Jiang, H.; Xie, P. Antifungal activities against Sclerotinia sclerotiorum by Cinnamomum cassia oil and its main
components. J. Essent. Oil Res. 2013, 25, 444451, doi:10.1080/10412905.2013.782475.
50. Al-Taisan, W.A.A.; Bahkali, A.H.; Elgorban, A.M.; El-Metwally, M.A. Effective influence of essential oils and microelements
against Sclerotinia sclerotiorum. Int. J. Pharmacol. 2014, 10, 275281, doi:10.3923/Ijp.2014.275.281.
51. Moraes, S.P.C.B.; Bucker, M.W.; Bucker, M.W.; De Resende, C.G.; Maciel, K.S.; De Lima, P.A.M. Cinnamon and citronella
essential oils in the In Vitro control of the fungi aspergillus sp. and Sclerotinia sclerotiorum. Afr. J. Agric. Res. 2018, 13, 18111815,
52. Combrinck, S.; Regnier, T.; Kamatou, G.P.P. In Vitro activity of eighteen essential oils and some major components against
common postharvest fungal pathogens of fruit. Ind. Crops Prod. 2011, 33, 344349, doi:10.1016/J.Indcrop.2010.11.011.
53. Kyu Kyu Win, N.; Jitareerat, P.; Kanlayanarat, S.; Sangchote, S. Effects of cinnamon extract, chitosan coating, hot water
treatment and their combinations on crown rot disease and quality of banana fruit. Postharvest Biol. Technol. 2007, 45, 333340,
54. Ojaghian, M.R.; Chen, Y.; Chen, S.; Cui, Z.-Q.; Xie, G.L.; Zhang, J. Antifungal and enzymatic evaluation of plant crude
extracts derived from cinnamon and rosemary against sclerotinia carrot rot. Ann. Appl. Biol. 2014, 164, 415429.
55. Mohammad, R.; Ojaghian, X.; Liang, Z.; Xiaolin, L.; Guan-Lin, X.; Jingze, Z.; Li, W. Effect of E-Cinnamaldehyde against
Sclerotinia sclerotiorum on potato and induction of glutathione s-transferase genes. Physiol. Mol. Plant Pathol. 2015, 91, 6671,
56. Fawzi, E.M.; Khalil, A.A.; Afifi, A.F. Antifungal effect of some plant extracts on Alternaria alternata and Fusarium oxysporum.
Afr. J. Biotechnol. 2009, 8, 25902597. www.Ajol.Info/Index.Php/Ajb/Article/View/60778.
Molecules 2021, 26, 5299 14 of 14
57. Imad, H.H.; Huda, J.A.; Ghaidaa, J.M. Evaluation of antifungal and antibacterial activity and analysis of bioactive
phytochemical compounds of Cinnamomum zeylanicum (Cinnamon Bark) using gas chromatography-mass spectrometry.
Orient. J. Chem. 2016, 32, 17691788, doi:10.13005/Ojc/320406.
58. Bravo, C.M.; Preston, G.M.; Renier, A.L.; Van Der Hoorn, R.A.; Flanagan, N.A.; Townley, H.E.; Thompson, I.P. Enhancing
cinnamon essential oil activity by nanoparticle encapsulation to control seed pathogens. Ind. Crops Prod. 2018, 124, 755764,
59. Il-Kwon, P.; Hoi-Seon, L.; Sang-Gil, L.; Ji-Doo, P.; Young-Joon, A. Insecticidal and fumigant activities of Cinnamomum cassia
bark-derived materials against Mechoris ursulus (Coleoptera: Attelabidae). J. Agric. Food Chem. 2000, 48, 25282531.
60. Pobożniak, M.; Grabowska,D.; Olczyk, M. Effect of orange and cinnamon oil on the occurrence and harmfulness of Thrips
tabaci Lind on onion—Preliminary results. Acta Horticulturae et Regiotecturae 2016, 19, 1314, doi:10.1515/Ahr-2016-0016.
61. Bueyong,P.; Myung-Ji, L.; Sang-Ku, L.;Sang-Bum,L.; In-Hong, J.; Se-Keun, P.; Ye-Jin, J.; Hoi-Seon, L. Insecticidal activity of
coriander and cinnamon oils prepared by various methods against three species of agricultural pests (Myzus persicae,
Tetranychus urticae and Plutella xylostella). Appl. Biol. Chem. 2017, 60, 137140, doi:10.3839/Jabc.2017.023.
62. Plata-Rueda, A.; Campos; J.M.; Rolim, G.S.; Martínez, L.C.; Santos, M.H.D. Terpenoid constituents of cinnamon and clove
essential oils cause toxic effects and behavior repellency response on granary weevil, Sitophilus granarius. Ecotoxicol. Environ.
Saf. 2018, 156, 263270, doi:10.1016/J.Ecoenv.2018.03.033.
63. Jumbo, L.O.V.; Faroni, L.R.A.; Oliveira, E.E.; Pimentel, M.A.; Silva, G.N. Potential use of clove and cinnamon essential oils to
control the bean weevil, Acanthoscelides obtectus say in small strong units. Ind. Crops Prod. 2014, 56, 2734.
64. Soon-Il, K.; Jung-Yeon, R.; Do-Hyoung, K.; Han-Seung, L.; Young-Joon, A. Insecticidal activities of aromatic plant extracts and
essential oils against Sitophilus oryzae and Callosobruchus chinensis. J. Stored Prod. Res. 2003, 39, 293303,
65. Szelényi, M.O.; Erdei, A.L.; Jósvai, J.K.; Radványi, D.; Sümegi, B.; Vétek, G.; Molnár, B.P.; Kárpáti, Z. Essential oil headspace
volatiles prevent invasive box tree moth (Cydalima perspectalis) ovipositionInsights from electrophysiology and behaviour.
Insects 2020, 11, 465, doi:10.3390/Insects11080465.
66. Kong, J.-O.; Lee, S.-M.; Moon, Y.-S.; Lee, S.-G.; Ahn, Y.-J. Nematicidal activity of cassia and cinnamon oil compounds and
related compounds toward Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae). J. Nematol. 2007, 39, 3136.
67. Orzeszko-Rywka, A.; Rochalska, M.; Lewicka, J. Wpływ zaprawiania nasion olejkiem cynamonowym i olejkiem z drzewa
herbacianego na wschody polowe i plonowanie pietruszki i sałaty. J. Agric. Eng. Res. 2012, 57, 5258.
68. Cavalieri, A.; Caporali, F. Effect of essential oils of cinnamon, lavender and peppermint on germination of mediterranean
weeds. Allelopath. J. 2010, 25, 441451.
69. The EU Green DealA Roadmap to Sustainable Economies. Available online: (accessed on 3 August 2021).
ResearchGate has not been able to resolve any citations for this publication.
Full-text available
Salmonella typhimurium infection is associated with gastrointestinal disorder and cellular injury in the liver of both humans and animals. Cinnamaldehyde, the main component of essential oil from cinnamon, has been reported to have anti-inflammatory, anti-oxidative, and anti-apoptotic effects. However, it remains unknown whether cinnamaldehyde can alleviate Salmonella typhimurium infection-induced liver injury in mice. In the present study, we found that cinnamaldehyde attenuated Salmonella typhimurium-induced body weight loss, the increase of organ (liver and spleen) indexes, hepatocyte apoptosis, and the mortality rate in mice. Further study showed that cinnamaldehyde significantly alleviated Salmonella typhimurium-induced liver injury as shown by activities of alanine transaminase, aspartate transaminase, and myeloperoxidase, as well as malondialdehyde. The increased mRNA level of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ) and chemokines (CCL2 and CCL3) induced by Salmonella typhimurium were significantly abolished by cinnamaldehyde supplementation. These alterations were associated with a regulatory effect of cinnamaldehyde on TLR2, TLR4, and MyD88. 16S rDNA sequence analysis showed that Salmonella typhimurium infection led to upregulation of the abundances of genera Akkermansia, Bacteroides, Alistipes, Muribaculum, and Prevotellaceae UCG-001, and downregulation of the abundances of genera Lactobacillus, Enterorhabdus, and Eggerthellaceae (unclassified). These alterations were reversed by cinnamaldehyde supplementation. In conclusion, cinnamaldehyde attenuated the inflammatory response, oxidative stress, and apoptosis in the liver of Salmonella typhimurium-infected mice. Supplementation of cinnamaldehyde might be a preventive strategy to alleviate liver injury caused by Salmonella typhimurium infection in humans and animals.
Full-text available
The emergence of antimicrobial resistance (AMR) has urged researchers to explore therapeutic alternatives, one of which includes the use of natural plant products such as essential oils (EO). In fact, EO obtained from clove, oregano, thymus, cinnamon bark, rosemary, eucalyptus, and lavender have been shown to present significant inhibitory effects on bacteria, fungi, and viruses; many studies have been done to measure EO efficacy against microorganisms. The strategy of combinatory effects via conventional and non-conventional methods revealed that the combined effects of EO–EO or EO–antibiotic exhibit enhanced efficacy. This paper aims to review the antimicrobial effects of EO, modes of EO action (membrane disruption, efflux inhibition, increase membrane permeability, and decrease in intracellular ATP), and their compounds’ potential as effective agents against bacteria, fungi, and viruses. It is hoped that the integration of EO applications in this work can be used to consider EO for future clinical applications.
Full-text available
The box tree moth (Cydalima perspectalis Walker) is an invasive species in Europe causing severe damage both in natural and ornamental boxwood (Buxus spp.) vegetation. Pest management tactics are often based on the use of chemical insecticides, whereas environmentally-friendly control solutions are not available against this insect. The application of essential oils may provide effective protection against oviposition and subsequent larval damage. Oviposition deterrence of cinnamon, eucalyptus and lavender essential oils was tested on female C. perspectalis in behavioural bioassays. Our results indicate that all the studied essential oils may be adequate deterrents; however, cinnamon oil exhibited the strongest effect. To determine the physiologically active compounds in the headspace of the essential oils, gas chromatography coupled with electroantennography recordings were performed in parallel with gas chromatography-mass spectrometry to identify the volatile constituents. In addition, the release rates of various components from vial-wick dispensers were measured during the oviposition bioassay. These results may serve as a basis for the development of a practical and insecticide-free plant protection method against this invasive moth species.
Full-text available
Biofungicides from plants are a possibility for the biocontrol of fungal diseases, as chemical products may be harmful to the environment and humans. Strawberry is one of the many plants infected by grey mould (Botrytis cinerea), and innovative methods of biocontrol against B. cinerea are under investigation. Clove (Syzygium aromaticum L.) and cinnamon (Cinnamomum cassia L.) accumulate natural compounds, such as eugenol and cinnamaldehyde, which provide antimicrobial and antifungal properties; thus, extracts of these plants could be possibly used as biofungicides. During this study, the inhibition of B. cinerea by clove and cinnamon extracts was evaluated in vitro on Petri plates and detached strawberry leaves; additionally, the chemical composition of volatiles was identified. Clove extract consisted of 52.88% eugenol, and cinnamon consisted of 74.67% cinnamaldehyde. The efficacy of the extracts on detached strawberry leaves showed that 12 mL L−1 concentration of clove extract was effective in suppressing the grey mould infection. Clove and cinnamon extracts showed an equal ability to inhibit B. cinerea on Petri plates. However, the results of the detached strawberry leaves assay showed that clove extract was more effective as a biocontrol product. Overall, clove extract expressed a high potential for application in biofungicides formulations.
Full-text available
In this study, the effects of organic powder of Cinnamomum zeylanicum on the development of Botrytis cinerea and its influence on tomato plants were evaluated. The cinnamon bark powder and its water suspensions and filtrates were used at 0.5 and 1% rates. After 6 days of the start of an in vitro experiment the mycelium growth was inhibited by both 0.5 and 1% cinnamon water filtrates - to a greater degree in the case of the higher concentration, by 54.4 and 81.4%, respectively. Spraying with cinnamon water filtrates positively influenced the growth of plants both in the greenhouse and the field. Antifungal activity of cinnamon was proved in the greenhouse tests - the disease symptoms of grey mould on infected tomato plants decreased. The fresh weight of non-inoculated tomato plants treated with cinnamon filtrates was significantly higher than control plants (17.17 g compared to 12.83 g) showing a stimulating effect of cinnamon filtrates. In the case of inoculated plants due to treatment, their weight increased from 7.83 to 10.50 g. In the field experiment, tomato plants sprayed six times with cinnamon were better developed than the control plants. The most significant effect was observed for Hamlet variety - the mean number of leaves was higher by 27.3% and the mean number of branches by 19.7% compared to the untreated control plants. Thus it was proved that cinnamon powder has potential to inhibit B. cinerea growth and also has a stimulating effect for tomato plants.
Full-text available
Cinnamon essential oil (CEO) has proven activity against several plant-pathogenic fungi and can reduce fungal diseases to an extent comparable to that achieved using commercial fungicides. Thus, the goal of the present work was to identify the active constituents of this essential oil and determine the direct fungicidal mode of action as well as putative indirect action by activation of citrus plant resistance against Alternaria brown spot (ABS). Gas chromatography-mass spec-trometry (GC-MS) of essential oil extracted from Cinnamomum zeylanicum leaves resulted in the identification of the active constituents eugenol and trans-cinnamaldehyde, which had, respectively, minimal in-hibitory concentrations (MICs) of 250 and 62.5 μg mL −1 against Alternaria alternata, while, under the same conditions, the MICs for a commercial fungicide and CEO were 1250 and 500 μg mL −1 respectively. Fungal growth inhibition assays showed that eugenol and trans-cinnamaldehyde (CNE) prevent fungal growth by direct fungicidal action and proved that CNE is mainly responsible for the anti-fungal activity of C. zeylanicum essential oil (CEO). CEO decreased the incidence of ABS disease to a greater extent than a commercial copper fungicide and was comparable to a commercial plant activator. Both CEO and its active constituent showed the ability to activate plant defense enzymes, demonstrating a high capacity to induce the citrus plant defense response. Therefore, both the essential oil and its active constituent have potential use in the development of new organic structures and analogues to control ABS disease caused by A. alternata.
Full-text available
Klebsiella pneumoniae (KP) remains the most prevalent nosocomial pathogen and carries the carbapenemase (KPC) gene which confers resistance towards carbapenem. Thus, it is necessary to discover novel antimicrobials to address the issue of antimicrobial resistance in such pathogens. Natural products such as essential oils are a promising source due to their complex composition. Essential oils have been shown to be effective against pathogens, but the overall mechanisms have yet to be fully explained. Understanding the molecular mechanisms of essential oil towards KPC-KP cells would provide a deeper understanding of their potential use in clinical settings. Therefore, we aimed to investigate the mode of action of essential oil against KPC-KP cells from a proteomic perspective by comparing the overall proteome profile of KPC-KP cells treated with cinnamon bark (Cinnamomum verum J. Presl) essential oil (CBO) at their sub-inhibitory concentration of 0.08% (v/v). A total of 384 proteins were successfully identified from the non-treated cells, whereas only 242 proteins were identified from the CBO-treated cells. Proteins were then categorized based on their biological processes, cellular components and molecular function prior to pathway analysis. Pathway analysis showed that CBO induced oxidative stress in the KPC-KP cells as indicated by the abundance of oxidative stress regulator proteins such as glycyl radical cofactor, catalase peroxidase and DNA mismatch repair protein. Oxidative stress is likely to oxidize and disrupt the bacterial membrane as shown by the loss of major membrane proteins. Several genes selected for qRT-PCR analysis validated the proteomic profile and were congruent with the proteomic abundance profiles. In conclusion, KPC-KP cells exposed to CBO undergo oxidative stress that eventually disrupts the bacterial membrane possibly via interaction with the phospholipid bilayer. Interestingly, several pathways involved in the bacterial membrane repair system were also affected by oxidative stress, contributing to the loss of cells viability.
The genus Cinnamomum includes a number of plant species largely used as food, food additives and spices for a long time. Different traditional healing systems have used these plants as herbal remedies to cure diverse ailments. The aim of this comprehensive and updated review is to summarize the biodiversity of the genus Cinnamomum, its bioactive compounds, the mechanisms that underlie the pharmacological activities and toxicological safety. All the data in this review have been collected from databases and recent scientific literature including Web of Science, PubMed, ScienceDirect etc. The results showed that the bioactive compounds of Cinnamomum species possess antimicrobial, antidiabetic, antioxidant, anti-inflammatory, anticancer and neuroprotective effects. The preclinical (in vitro/in vivo) studies provided the possible molecular mechanisms of these action. As a novelty, recent clinical studies described in this paper support and confirm the pharmacological importance of the genus Cinnamomum. In conclusion, the obtained results from preclinical studies and clinical trials, as well as reduced side effects provide insights into future research of new drugs based on extracts and bioactive compounds from Cinnamomum plants.
The insecticidal and fumigant activities of Cinnamomum cassia (Blume) bark-derived materials against the oak nut weevil (Mechoris ursulus Roelofs) were examined using filter paper diffusion and fumigation methods and compared to those of the commercially available Cinnamomum bark-derived compounds (eugenol, salicylaldehyde, trans-cinnamic acid, and cinnamyl alcohol). The biologically active constituent of the Cinnamomum bark was characterized as trans-cinnamaldehyde by spectroscopic analysis. In a test with the filter paper diffusion method, trans-cinnamaldehyde showed 100 and 83.3% mortality at rates of 2.5 and 1.0 mg/filter paper, respectively. At 2.5 mg/paper, strong insecticidal activity was produced from eugenol (90.0% mortality) and salicylaldehyde (88.9%), whereas trans-cinnamic acid revealed moderate activity (73.3%). At 5 mg/paper, weak insecticidal activity (50.0%) was produced from cinnamyl alcohol. In a fumigation test, the Cinnamomum bark-derived compounds were much more effective against M. ursulus larvae in closed cups than in open ones. These results indicate that the insecticidal activity of test compounds was attributable to fumigant action, although there is also significant contact toxicity. As a naturally occurring insect-control agent, the Cinnamomum bark-derived materials described could be useful as a new preventive agent against damage caused by M. ursulus.