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Grass pollen sensitization in dogs in Paraná, Brazil: comparison between percutaneous and intradermal testing



Canine atopic dermatitis is an inflammatory, genetic, pruritic and chronic dermatosis that affects between 10 and 30% of dogs and one of the most important allergens is grass pollen. The objective of this study was to evaluate the sensitization to grass pollen allergens in dogs with canine atopic dermatitis and to compare intradermal skin test (IDT) with percutaneous test (PT). For this study, ten healthy dogs and 39 dogs with atopic dermatitis were tested. Dogs were submitted to IDT and PT for Lolium multiflorum, Cynodon dactylon and Paspalum notatum. The IDT and PT tests were compared using the Proportion Test. All healthy dogs were negative to both tests. Ten atopic dogs (25.6%) responded positively to the PT and none were positive in IDT. C. dactylon, L. multiflorum and P. notatum were responsible for positive reactions in 70%, 70% and 30% of positive dogs, respectively. The number of positive reactions in PT were statistically higher than IDT (P<0.05). In conclusion, grass pollen can be important source of allergens for dogs in Paraná state (Brazil) and the PT showed higher sensitization to grass pollen in dogs with atopic dermatitis than IDT. Keywords: dogs; grass pollen; atopic dermatitis; prick test
Arq. Bras. Med. Vet. Zootec., v.73, n.4, p.821-826, 2021
Grass pollen sensitization in dogs in Paraná, Brazil: comparison between
percutaneous and intradermal testing
[Sensibilização a polens em cães no Paraná, Brasil: comparação entre o teste percutâneo
e o teste intradérmico]
A.P.S. Cerdeiro1, M.R. Farias2*, V.E.S. Cunha3, G.V. Secchi1, B.D. Pacheco1,
C.O.R. Alcalá1, R.G.R. Ganho4
1Aluna de pós-graduação ˗ Pontifícia Universidade Católica do Paraná ˗ Curitiba, PR
2 Pontifícia Universidade Católica do Paraná ˗ Curitiba, PR
3FDA Allergenic ˗ Rio de Janeiro, RJ
4Aluno de pós-graduação ˗ Pontifícia Universidade Católica do Paraná ˗ Curitiba, PR
Canine atopic dermatitis is an inflammatory, genetic, pruritic and chronic dermatosis that affects between
10 and 30% of dogs and one of the most important allergens is grass pollen. The objective of this study was
to evaluate the sensitization to grass pollen allergens in dogs with canine atopic dermatitis and to compare
intradermal skin test (IDT) with percutaneous test (PT). For this study, ten healthy dogs and 39 dogs with
atopic dermatitis were tested. Dogs were submitted to IDT and PT for Lolium multiflorum, Cynodon
dactylon and Paspalum notatum. The IDT and PT tests were compared using the Proportion Test. All
healthy dogs were negative to both tests. Ten atopic dogs (25.6%) responded positively to the PT and none
were positive in IDT. C. dactylon, L. multiflorum and P. notatum were responsible for positive reactions in
70%, 70% and 30% of positive dogs, respectively. The number of positive reactions in PT were statistically
higher than IDT (P<0.05). In conclusion, grass pollen can be important source of allergens for dogs in
Paraná state (Brazil) and the PT showed higher sensitization to grass pollen in dogs with atopic dermatitis
than IDT.
Keywords: dogs, grass pollen, atopic dermatitis, prick test
A dermatite atópica canina é uma dermatose inflamatória, genética, prurítica e crônica que afeta entre 10% e
30% dos cães, e um dos alérgenos mais importantes são os polens de gramíneas. O objetivo deste estudo é
avaliar a sensibilização a alérgenos de polens de gramíneas em cães com dermatite atópica e comparar o teste
intradérmico (TID) com o teste percutâneo (TP). Para o estudo, 10 cães hígidos e 39 cães com dermatite atópica
foram testados. Estes foram submetidos ao TID e ao TP para Lolium multiflorum, Cynodon dactylon e Paspalum
notatum. TID e TP foram comparados usando-se o teste de proporção. Todos os cães hígidos foram negativos
em ambos os testes. Dez cães atópicos (25,6%) responderam positivamente ao TP e nenhum ao TID. C. dactylon,
L. multiflorum e P. notatum foram responsáveis por reações positivas de 70%, 70% e 30% dos cães positivos,
respectivamente. O número de reações positivas no TP foi estatisticamente maior que no TID (P<0,05). Foi
concluído que os polens de gramíneas podem ser importantes fontes de alérgenos para cães no estado do Paraná
(Brasil) e que o TP mostrou maior sensibilização a polens em cães com dermatite atópica que o TID.
Palavras-chave: cães, polens de gramíneas, dermatite atópica, teste percutâneo
Canine atopic dermatitis (CAD) is a pruritic,
inflammatory, and chronic dermatopathy, with a
genetic predisposition, affecting between 10 and
Recebido em 29 de junho de 2020
Aceito em 6 de abril de 2021
*Autor para correspondência (corresponding author)
30% of the canine population (Carlotti, 2012).
CAD results in the loss of the integumentary
physical barrier function and increased
immunoreactivity. It is estimated that
approximately 80% of dogs with CAD have
A.P.S. Cerdeiro
M.R. Farias
V.E.S. Cunha
G.V. Secchi
B.D. Pacheco
C.O.R. Alcalá
R.G.R. Ganho
Cerdeiro et al.
822 Arq. Bras. Med. Vet. Zootec., v.73, n.4, p.821-826, 2021
increased levels of the specific antibody
immunoglobulin E (IgE), mainly as a response to
environmental, microbial and food allergens
(Pucheu-Haston et al. 2015). The environmental
major allergens inducing CAD come from house
dust mite allergens, weeds, shrubs, and grasses
(Prélaud, 2014). In people with atopic dermatitis
IgE mediated by extrinsic factors, it was found
that exposure to grass pollens induces a
significant worsening of cutaneous symptoms
(Werfel et al., 2016).
The major allergenic grasses in south Brazil are
Lolium multiflorum (LM) or Ryegrass, Cynodon
dactylon (CD) or Bermuda grass, and Paspalum
notatum (PN) or Bahia Grass. The subfamily
Pooideae, to which belongs the LM, has the major
allergens in groups 1 and 5 and may confer cross-
reactivity with other species of the same
subfamily and with species of other subfamilies
(Vieira, 2012). Among them, Lolium multiflorum
is considered to be the most important allergen in
humans in south Brazil (Rosário Filho, 2012).
Veterinary studies to verify the incidence of
pollinosis in Brazil are rare. A recent study that
used intradermal skin test (IDT) in 58 dogs with
atopic dermatitis in Rio Grande do Sul state,
demonstrated a positive response to CD (24.13%),
and PN (12%) (Pereira et al., 2015). Sensitization
to LM was not evaluated.
Percutaneos test (PT) has been used in medicine
since its first description by Jack Pepys in 1975 as
an alternative with lower risk of systemic
reactions compared to IDT. Studies performed in
cats and dogs showed papule reactions easier to
interpret, no cutaneous and systemic reactions and
good specificity (Rocha, 2012; Rossi et al., 2013,
Gentry e Messinger, 2016). The objective of this
study was to evaluate the sensitization to grass
pollen allergens in dogs with CAD and to compare
IDT with PT.
The present study was approved by the Ethics
Committee for the Use of Animals at the
Pontifical Catholic University of Paraná (registry
number: 1027/2016). It was a cross-sectional,
non-randomized and controlled study. Ten
healthy dogs, 5 females and 5 males, average age
of 5 years, and 39 dogs with atopic dermatitis, 26
females, average age of 5 years, irrespective of
breed, from the Pontifical Catholic University of
Paraná Veterinary Clinic School were included.
There were not included in this study dogs that
received antihistamines (1 week), oral and topical
glucocorticoids (2 weeks) or long-term injectable
glucocorticoids (4 weeks), according to Olivry’s
study of anti-allergic drugs optimal and minimal
withdrawal times before allergic tests in dogs
(2013). Females in estrus, gestation or lactation
and dogs with chronic systemic diseases or with
any other dermatopathy were also not included.
All dogs in this study were submitted to the IDT
and PT sequentially in the same day by the same
examinator, who previously did a proficiency test
as described by Cunha et al. (2015).
For IDT, the skin was shaved using a grooming
machine and blade 40 in the lateral region of the
thorax. After careful cleaning of the skin with a
physiological solution, 10 application points were
marked at an interval of 3cm with a dermographic
pen. For both groups, 0.9% phenolated saline and
a histamine solution (0.05 mg/ml) were used as a
negative and positive controls, respectively.
Sensitization to pollen was evaluated using
standardized extracts of LM, CD and PN at the
concentration of 100 UBE /ml. All extracts were
supplied by FDA Allergenic (Rio de Janeiro,
Brazil). The intradermal applications were
performed using 0.5-ml insulin syringes, attached
to hypodermic needles (13 x 4,5mm), containing
0.05ml of each grass pollen extract. Fifteen
minutes after application, the papules were
marked with a dermographic pen to facilitate the
reading. The cut-off value was established by
calculating the mean for the negative control (CN)
and positive control (CP) values. The responses to
the tested antigens were considered positive when
the mean diameter of the papules was ≥ the
calculated cut-off value.
In the PT, histamine hydrochloride (10 mg/ml)
and saline (containing 0.45% phenol) were used
as the positive and negative control, respectively.
Glycerinated allergenic extracts of LM, CD and
PN were used at the concentration of 10.000
UBE/mL. All the extracts were supplied by FDA
Allergenic (Rio de Janeiro, Brazil). After
applying a drop of each extract or control
solutions on the skin, the device (Duotip-test II®,
Multi-test Brazil) was passed through the drop at
a 45° to 60° angle to the skin. The skin was then
gently lifted, creating a small break in the
epidermis through which the suspected allergen
solution penetrates. Then an absorbent paper was
Grass pollen sensitization…
Arq. Bras. Med. Vet. Zootec., v.73, n.4, p.821-826, 2021 823
used to remove the excess of solutions. After 15
minutes, the test sites were observed, and the
papules were evaluated. Papules that were at least
3mm larger than the negative control were
considered positive (Van der Valk et al., 2016).
The results of the PT and IDT were compared in
a descriptive way and then by the proportion test
using the Minitab16 software (Minitab Inc., State
College, Pennsylvania), with significance level
set at α=0.05 (P≤0.05).
All dogs in Group 1 were negative to the allergen
extracts in the IDT and PT. The mean histamine
papule diameter in the IDT and PT was 15 and
7.45mm, respectively. The positive reactions in
PT ranged from 3 to 6mm, with formation of
pseudopods in some cases. The comparison
between papular histamine reaction in IDT and
positive reaction to grass pollen in PT is shown in
Figure 1. In DAC group, none of the dogs were
positive for the pollen tested in IDT. Ten dogs
(25.6%) reacted to grass pollen with PT. Seven
dogs (18%) were positive to CD and LM, while
three dogs (7.7%) were positive to PN. Two dogs
(5.1%) reacted to three species tested and three
dogs (7.7%) to two species. Monosensitization
was observed in five dogs (12.8%) as shown in
Table 1. The statistical analysis with Proportion
Test showed that the PT was more effective in
identifying sensitization to CD and LM
(p=0,002), and PN (p=0,036) compared to IDT.
Figure 1. Comparison of papular histamine reaction in the intradermal test (left) and positive for grass
pollen in the prick test (right) in millimeters. Note the formation of a pseudopod (arrow).
Table 1. Results of sensitization among ten reactive dogs in to grass pollen through the Percutaneous Test
CD Cynodon dactylon; LM Lolium multiflorum; PN Paspalum notatum.
In the present study, the PT and IDT showed high
specificity, justified by the lack of positive
animals in the control group; these solutions were
tested previously for irritative threshold in healthy
dogs (unpublished data) and were tested again in
this study to prove these findings. It is believed
that IDT based on the irritation threshold have
high specificity in the diagnosis of allergic
sensitization (Hensel et al., 2004). It is estimated
that the pollen sensitization in allergic people in
Brazil varies from 16% to 34%, being more
prevalent in the South and Center-West of the
country (Vieira et al., 2011; Vieira, 2014;
Sopelete et al., 2014). In dogs, the prevalence of
grass pollen sensitization ranges from 10 to 27.7%
(Masuda et al., 2000; Roussel et al., 2013). In
Brazil, one study using IDT identified 10.3% of
sensitization in atopic dogs using grass polens
extracts (Pereira et al., 2015). In the present study
using PT, the prevalence of sensitization to grass
pollen was (25.6%) and CD and LM were the
most important species.
Cerdeiro et al.
824 Arq. Bras. Med. Vet. Zootec., v.73, n.4, p.821-826, 2021
This could be explained by the wide distribution
of these species in the South region of Brazil,
which can be found along roads, backyards, and
parks (Viera, 2012). LM may provoke rhino
conjunctivitis and allergic asthma in people
(Rosário Filho, 2012) due to its wide distribution
and an increase in its cultivation over the years. It
has high allergenic potential due to its large-scale
production and rapid spread in the wind
(Bonissoni and Neto, 2014). In Curitiba, South of
Brazil, a study done in 1999 found positive results
using PT for LM in 4.7% of children aged 13 to
14 years and 15.4% of adults over 20 years of age
(Esteves et al., 1999). A study conducted in 2002
found positivity for Lolium perene of 16.5% in
skin allergic tests (Rosário Filho, 2002). In
veterinary medicine, the incidence of sensitization
to LM has not been evaluated so far and the
present study revealed a positive response to this
grass among atopic dogs (12.8%) similar to
previous findings.
Additionally, other species such as CD are
allergenic and produce allergy symptoms beyond
September and December (Rosário Filho, 2012).
Sensitization to CD was detected in 16.8 and 80%
of patients with rhinitis in Curitiba (Araújo et al.,
2015) and Rio Grande do Sul (Vieira et al..,
2011), respectively. A previous study using IDT
in Brazil found that 24.13% of atopic dogs were
sensitized to CD (Pereira et al., 2015). In the
present study, the positive response to this grass
was similar to the other studies cited. The lowest
incidence of positive response was observed with
PN. Positive reactions to PN were accompanied
by positive reactions to CD, suggesting the
possibility of cross-reaction between these
species. In dogs, the major allergens of grasses are
yet to be identified; hence we could not test the
hypothesis of cross-reaction between and PN in
this study.
Among the ten animals with positive pollen
responses through PT, 2 were positive only to CD,
2 to CD and LM, 2 to CD, LM and PN, 3 only LM
and 1 to CD and PN. Therefore, there was no
pattern of response suggestive of cross reactivity.
The exception was the 3 PN positive dogs that
were also CD positive. However, 4 dogs were CD
positive but not PN positive. These results may
indicate true sensitization to both species and
cross reactivity between species, however
suggesting that CD extracts may contain their own
antigens. Due to the small number of positive tests
(n=10) and the heterogeneous pattern of
responses, it was not possible to perform an
adequate statistical analysis for evaluation of
cross-reactivity among the species tested. For
cross-reactivity studies, the ideal would be the use
of immunochemical methods such as Cross-
Inhibition ELISA, which is beyond the scope of
this study.
The negative results in the present study can be
explained by the sensitization to other
environmental allergens, mainly from house dust
mites species, species, as Dermatophagoides
farinae, D. pteronyssinus and Blomia tropicalis, as
shown by Cunha et al. in studies done in Brazil
(Cunha et al., 2007; Cunha, 2012). Besides that, it
is estimated that about 30% of patients with
clinical diagnosis of atopic dermatitis have
negative skin tests (Bauer et al., 2010). This
suggests an innate, cellular inflammatory
component that mediates pruritus development
and symptoms related to atopic dermatitis in some
patients, with no clear relation with sensitization
and IgE formation (Halliwell, 2006). These
findings could also explain some negative results
observed. No systemic or local adverse reactions
were observed in the PT and IDT, suggesting the
safety of these tests in dogs. Some dogs presented
only local effects characterized by pruritus and
erythema, which disappeared in a few hours. In
humans, the PT generates fewer adverse reactions
than IDT, and fatal reactions can occur in the latter
(Bernstein et al., 2008).
The prick test was more effective in identifying
grass pollen sensitization in dogs with atopic
dermatitis than the intradermal skin test.
Sensitization to grass pollen occurred in about a
quarter of the subjects and was more common to
CD and LM through the prick test. Future studies
are recommended to evaluate the efficacy of
allergen-specific immunotherapy in dogs
sensitized to these grass species.
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... Trees, weeds, and grass also contribute to sensitization [34]. Our findings (Figure-7) revealed that oak (34.6%), white ash (21.6%), maple leaf sycamore (18.2), sheep's sorrel (25.0%), mugwort (18.0%), ...
Full-text available
Background and aim: Canine atopic dermatitis (CAD) is a hereditary susceptibility to the development of allergic symptoms in response to repeated exposure to generally innocuous substances known as "allergens." Allergens can be plants, animals, mold, mites, or milk. At present, serological enzyme-linked immunoassay (ELISA) kits are used for immunoglobulin E (IgE)-specific allergen detection due to their simplicity and accuracy. This study aimed to detect allergens in dogs with CAD and determine how they differ according to season, breed, age, and sex using a serological test in six provinces in South Korea for 12 months. This will allow practitioners to easily understand the risk factors related to CAD. Materials and methods: In this study, IgE allergen-specific ELISA kits were used. The allergens were detected in serum samples collected from different regions considering season, sex, breed, and age. Allergens were divided into the following Ten categories: 1. Dairy, yeast, and egg, 2. grains, 3. vegetables, 4. meat, 5. seafood, 6. animals, 7. mold, 8. insects, 9. mites, and 10. trees. Results: The percentage of allergens detected in males (54.8%) was higher than that of females (45.2%); 54.2% of allergens occurred in 3-year-old dogs or older. Moreover, regarding frequency, 65.6% of overall allergens occur during autumn; Chungcheongnam-do and Jeollabuk-do showed 20.7% and 20.9%, respectively. Additionally, among allergens categories, notable allergen occurrence was as follows: 38.3% corn; 28.7% potatoes; 22.7% duck; 24.4%,codfish; 31.2% animal wool; 95.6% Aspergillus fumigatus; 31.9% flea; 41.8% oak; and 25.0% sheep's sorrel grass. Conclusion: This study showcases the frequency of 60 allergens in six provinces detected in dogs with CAD; most likely from food or the environment using serological ELISA kits. Environmental sensitizer results can be considered for humans suffering from allergies to avoid a similar environment. A large-scale study can be performed to evaluate the allergens in the state. However, neither a skin test nor feed analysis was conducted, which is a limitation of this study.
Full-text available
Background: There are several methods to read skin prick test results in type-I allergy testing. A commonly used method is to characterize the wheal size by its ‘average diameter’. A more accurate method is to scan the area of the wheal to calculate the actual size. In both methods, skin prick test (SPT ) results can be corrected for histamine- sensitivity of the skin by dividing the results of the allergic reaction by the histamine control. The objectives of this study are to compare different techniques of quantifying SPT results, to determine a cut-off value for a positive SPT for histamine equivalent prick -index (HEP) area, and to study the accuracy of predicting cashew nut reactions in double- blind placebo-controlled food challenge (DBPCFC) tests with the different SPT methods. Methods: Data of 172 children with cashew nut sensitisation were used for the analysis. All patients underwent a DBPCFC with cashew nut. Per patient, the average diameter and scanned area of the wheal size were recorded. In addition, the same data for the histamine-induced wheal were collected for each patient. The accuracy in predicting the outcome of the DBPCFC using four different SPT readings (i.e. average diameter, area, HEP-index diameter, HEP- index area) were compared in a Receiver-Operating Characteristic (ROC) plot. Results: Characterizing the wheal size by the average diameter method is inaccurate compared to scanning method. A wheal average diameter of 3 mm is generally considered as a positive SPT cut-off value and an equivalent HEP- index area cut-off value of 0.4 was calculated. The four SPT methods yielded a comparable area under the curve (AUC) of 0.84, 0.85, 0.83 and 0.83, respectively. The four methods showed comparable accuracy in predicting cashew nut reactions in a DBPCFC. Conclusions: The ‘scanned area method’ is theoretically more accurate in determining the wheal area than the ‘average diameter method’ and is recommended in academic research. A HEP-index area of 0.4 is determined as cut-off value for a positive SPT. However, in clinical practice, the ‘average diameter method’ is also useful, because this method provides similar accuracy in predicting cashew nut allergic reactions in the DBPCFC. Trial registration: Trial number NTR3572
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A dermatite atópica canina (DAC) é uma dermatose comum, definida como doença de cunho genético que predispõe à inflamação e ao prurido cutâneo, associados à resposta IgE específica na maior parte dos casos. O diagnóstico da DAC é clínico e pode ser posteriormente complementado por testes alérgicos cutâneos e/ou sorológicos. O objetivo desses testes é identificar possíveis alérgenos e, com isso, possibilitar ao clínico a seleção de antígenos candidatos para a imunoterapia alérgeno-específica. No presente estudo, foram testados 58 animais diagnosticados para DAC. Todos os animais foram submetidos ao teste cutâneo intradérmico (TID), e amostras de sangue foram coletadas para a realização de testes sorológicos. Os resultados obtidos demonstraram elevada prevalência de sensibilização aos ácaros domiciliares e ao pólen da gramínea C. dactylon nos cães testados. Com este trabalho, foi possível identificar os principais alérgenos envolvidos na resposta imunológica de cães atópicos residentes na região central do Rio Grande do Sul.
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House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.
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The objective of the study was to evaluate whether allergenic extracts of five house dust and storage mite species standardized for humans might be used for the diagnosis of canine atopic dermatitis (CAD). Extracts of Dermatophagoides pteronyssinus (Pyroglyphidae), D. farinae (Pyroglyphidae), Blomia tropicalis (Glycyphagidae), Lepidoglyphus destructor (Glycyphagidae) and Tyrophagus putrescentiae (Acaridae) were evaluated by intradermal testing in 20 healthy dogs (control) and 25 dogs with allergic dermatitis. A significant difference in the response was observed between the two groups (p<0.05). Only one dog (5%) in the control group reacted to the intradermal test, whereas 14 dogs (56%) in the allergic group were positive for at least one extract (odds ratio = 24.2). Most of the positive reactions observed in the allergic group occurred against the extracts of T. putrescentiae or L. destructor, each inducing reactions in 10 dogs (40%). D. farinae, D. pteronyssinus e B. tropicalis extracts induced reactions in 7 (28%), 3 (12%) and 3 (12%) dogs, respectively. The allergenic extracts standardized for humans evaluated in the present study may be used as a tool to complement the diagnosis of the disease, as well as to select potential allergen candidates for allergen-specific immunotherapy.
Background: Intradermal testing (IDT) in cats has potential limitations; this has led to an interest in novel testing methods. A pilot study demonstrated that healthy cats produced reliable percutaneous glycerinated (PG) histamine wheals, whereas percutaneously applied glycerosaline did not lead to wheal formation. Hypothesis/objective: The purpose of this study was to determine if percutaneously applied aqueous and glycerinated allergens would lead to irritant reactions in healthy cats. Methods: Percutaneous testing (PCT) with both glycerinated and aqueous allergens and IDT were compared in twelve healthy cats. The lateral thorax was clipped and histamine, saline and nine allergens were tested in rows. Objective and subjective evaluations were performed at 15, 20 and 25 min, and 4 h. Results were evaluated as positive or negative at 15, 20, 25 min and 4 h. Results: Skin test reactions for intradermal (ID) histamine wheals were larger when compared to PG and percutaneous aqueous (PA) at the immediate reading points (P < 0.05) subjectively and objectively; however, PG was not significantly different from ID when compared as either positive (2-4) or negative (0-1). PG histamine and allergen reactions, when present, were larger than equivalent PA reactions. PG and PA allergens did not cause irritant reactions at tested concentrations. Bassia scoparia (kochia), when tested at 1000 PNU/mL with IDT, was suspected to be an irritant. Conclusions and clinical importance: Percutaneously (PCT) applied allergens did not cause irritant reactions in healthy cats. PG histamine wheals, although smaller than ID histamine wheals, were easily recognizable and PCT was simple to perform.
It has frequently been speculated that pruritus and skin lesions develop after topical exposure to aeroallergens in sensitized patients with atopic dermatitis (AD). We sought to study cutaneous reactions to grass pollen in adult patients with AD with accompanying clear IgE sensitization to grass allergen in an environmental challenge chamber using a monocenter, double-blind, placebo-controlled study design. Subjects were challenged on 2 consecutive days with either 4000 pollen grains/m(3) of Dactylis glomerata pollen or clean air. The severity of AD was assessed at each study visit up to 5 days after challenge by (objective) scoring of AD (SCORAD). Additionally, air-exposed and non-air-exposed skin areas were each scored using local SCORAD scoring and investigator global assessments. Levels of a series of serum cytokines and chemokines were determined by using a Luminex-based immunoassay. The primary end point of the study was the change in objective SCORAD scores between prechallenge and postchallenge values. Exposure to grass pollen induced a significant worsening of AD. A pronounced eczema flare-up of air-exposed rather than covered skin areas occurred. In grass pollen-exposed subjects a significantly higher increase in CCL17, CCL22, and IL-4 serum levels was observed. This study demonstrates that controlled exposure to airborne allergens of patients with a so-called extrinsic IgE-mediated form of AD induced a worsening of cutaneous symptoms. Copyright © 2015 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.
Prevalence of respiratory allergic diseases has increased worldwide. Identification of the aeroallergens involved in allergenic sensitisation is important for diagnosis, treatment and prevention. To verify the molecular pattern of sensitisation to aeroallergens in patients with allergic respiratory diseases using microarray technique for specific IgE antibody detection. Cross-sectional study of 101 children with allergic rhinitis was followed in an outpatient clinic. All patients had positive skin prick tests (SPT) to at least one of the following antigens: Dermatophagoides pteronyssinus, Blomia tropicalis, Blattella germanica, Lolium multiflorum, and dog and cat epithelium. Serum specific IgE antibodies (sIgE) to mites, animal epithelia, fungi, cockroach and pollens components were determined by ImmunoCAP ISAC. sIgE to group 1 and 2 mite allergens showed higher positive rates: Der p 1 (74.2%), Der p 2 (73.3%), Der f 1 (74.2%), Der f 2 (72.3%). sIgE to animal epithelia were less frequent, Can f 1, Can f 2, Can f 3 in 4.9%, 2.9%, 1.9% respectively and Fel d 1, Fel d 2, Fel d 4 in 16.8%, 0.9% and 1.9%. respectively. Sensitisation to fungi and cockroach were rare, except for Bla g 7, to which 16.8% were positive. There was no significant recognition for tree pollens group. For grass, sIgE were detected to Cyn d 1 in 16.8%, Phl p 1 and Phl p 4 in 14.8% and 12.9%, respectively. Knowing that the pattern of allergic sensitisation varies according to environment and population, our results reinforce the need for local studies, using molecular-based diagnosis. Copyright © 2014 SEICAP. Published by Elsevier Espana. All rights reserved.
Canine atopic dermatitis (AD) is considered to be an immunoglobulin E (IgE)-mediated hypersensitivity response to environmental allergens. The role of other antibody isotypes and nonenvironmental allergens in disease pathogenesis remains unclear. The objective of this review is to provide an update on advances in the understanding of the relevance of specific antibody isotypes, autoallergens and nonenvironmental allergens in the pathogenesis of canine AD. Citation databases, abstracts and proceedings from international meetings published between 2001 and 2013 were reviewed. Where necessary, older articles were included for background information. Neither total nor allergen-specific IgE necessarily correlates with clinical disease in canine AD. Some dogs exhibit clinical signs that are indistinguishable from AD but have no demonstrable allergen-specific IgE (atopic-like dermatitis). Allergen-specific immunoglobulin G may be demonstrated in canine AD, but there is no evidence that this isotype plays a role in disease development. Although humans with AD may develop serum IgE against autoallergens, this finding has not been substantiated in the dog. In contrast, adverse food reactions are frequently co-associated with AD in the dog. Ingestion of food and environmental allergens may trigger exacerbations of AD. Determination of the role of IgE in the pathogenesis of canine AD still requires clarification. Clinical trials and research studies must distinguish atopic dogs with allergen-specific IgE or skin test reactivity from those without. There is no convincing evidence demonstrating a pathogenic role for either allergen-specific immunoglobulin G or autoallergens in canine AD, but food items may be triggers for disease flares in certain individuals. © 2015 ESVD and ACVD.
218 Prezado Editor, As diversas espécies de gramíneas da família Poaceae (Gramineae) estão largamente distribuídas ao redor do mundo e permanecem responsáveis pela maioria dos casos de polinose. Um clássico representante no Sul do Brasil é o Lolium multiflorum (Lol m), pertencente à subfamília Pooideae, denominado azevém anual, considerado atu-almente nosso principal agente de polinose. Possui os maiores antígenos polínicos localizados nos grupos 1 e 5. É capaz de produzir elevada reatividade cruzada com seu homônimo Lolium perene (Lol p) identificado como azevém perene e com mistura de extratos polínicos de espécies pertencentes à subfamília Pooideae 1 . Essas são, em geral, oriundas de clima temperado europeu e distribuídas no Brasil por laboratórios "em pacotes", tanto para testes cutâneos quanto para imunoterapia específica. Cynodon dactylon (Cyn d) e Paspalum notatum (Pas n), de forma distinta, são gramíneas pertencentes, respectivamen-te, às subfamílias (Chloridoideae e Panicoideae), mantendo também elevados potenciais alergênicos. Ambas apresentam ausência ou diminuta sensibilização cruzada com a subfamília Pooideae, na qual se inclui o Lol m, possuem como principal alergeno o grupo 1, existindo a falta do grupo 5 2,3 . Esses conceitos devem ser relacionados tanto para diagnósticos como no tratamento 2 . Elas são consideradas excelentes pastagens e forrageiras que se adaptam ao calor, entretanto, podem difundir-se largamente como invasoras em outras áreas, tais como: beira de estradas, terrenos abandonados, lavouras de di-versas culturas, entre outras, e se comportar como uma "erva daninha".
Intradermal testing is useful for the identification of environmental allergens to which cats could be hypersensitive; intradermal test reactions are often subtle and difficult to interpret in cats. Percutaneous testing is the standard technique for the detection of significant environmental allergens in people, but it has not yet been evaluated in cats with hypersensitivity dermatitis. The purpose of this study was to evaluate and compare the skin test responses of healthy cats to percutaneous application and intradermal injections of control solutions. Ten clinically healthy cats were studied. Percutaneous applications of 0.0275 and 0.1 mg/mL aqueous histamine, 6 mg/mL glycerinated histamine, 0.9% buffered saline and 50% glycerosaline solution were performed using Greer Pick (Greer Laboratories, Lenoir, NC, USA) and Duotip-Test II (Lincoln Diagnostics, Decatur, IL, USA) percutaneous applicators. Reactions were compared with intradermal injections of 0.0275 mg/mL aqueous histamine and 0.9% buffered saline as controls. Positive responses to histamine solutions were significantly greater with the Greer Pick than with the Duotip-Test II. There were no significant differences between the histamine reactions using the Greer Pick applicator and the intradermal injections. Percutaneous reactions to histamine were more well demarcated and easier to read than intradermal injection reactions. Reactions to the saline controls were not noted. Percutaneous application of 6 mg/mL glycerinated histamine solution, 50% glycerosaline solution and 0.9% buffered saline produced similar positive and negative control wheals. These observations warrant further studies of percutaneous allergen testing in cats with hypersensitivity dermatitis.