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coatings
Article
In Vitro Microleakage Evaluation of Bioceramic and
Zinc-Eugenol Sealers with Two Obturation Techniques
Francesco De Angelis *, Camillo D’Arcangelo, Matteo Buonvivere, Rachele Argentino and Mirco Vadini
Citation: De Angelis, F.;
D’Arcangelo, C.; Buonvivere, M.;
Argentino, R.; Vadini, M. In Vitro
Microleakage Evaluation of
Bioceramic and Zinc-Eugenol Sealers
with Two Obturation Techniques.
Coatings 2021,11, 727. https://
doi.org/10.3390/coatings11060727
Academic Editor: Jasmina Primožiˇc
Received: 22 May 2021
Accepted: 15 June 2021
Published: 17 June 2021
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conditions of the Creative Commons
Attribution (CC BY) license (https://
creativecommons.org/licenses/by/
4.0/).
Unit of Restorative Dentistry and Endodontics, Department of Medical, Oral and Biotechnological Science,
School of Dentistry, “G. D’Annunzio” University of Chieti, 66100 Chieti, Italy; cdarcang@unich.it (C.D.);
matteo.buonvivere@unich.it (M.B.); rachele.argentino@studenti.unich.it (R.A.); m.vadini@unich.it (M.V.)
*Correspondence: f.deangelis@unich.it; Tel.: +39-(0)85-4549652
Abstract:
Aim of the study is to compare the quality of the apical seal offered by a zinc-eugenol and
a tricalcium-silicate-based sealer, both used with the single-cone or with the continuous wave of
condensation technique. Forty central incisors were divided into four groups (n = 10), according
to the two sealers and the two obturation techniques under investigation, and their outer surface
was isolated with nail varnish. After endodontic treatment, samples were immersed in methylene
blue dye for 72 h, then included in self-curing resin and sectioned to longitudinally expose the
canal apical third. The depth of dye penetration was measured in each group. Mean values were
compared by two-way-ANOVA test. Multiple comparisons were performed by Tukey test. The level
of significance was set at 0.05 in all tests. The continuous wave of condensation technique led to
reduced microleakage. Moreover, dye penetration values were reduced for the tricalcium-silicate
sealer. In terms of microleakage, the warm continuous wave of condensation technique seems
promising even when combined to a bioceramic sealer.
Keywords: bioceramic; continuous wave condensation; microleakage; single-cone; zinc-oxide-eugenol
1. Introduction
The ultimate aim of the root canal treatment, after an adequate disinfection carried out
by mechanical instruments [
1
] and chemical solutions [
2
–
5
], is to fill in a three-dimensional
way the endodontic space, in order to obtain a fluid-tight barrier, stable over time, with
the purpose of protecting the periradicular tissues from microorganisms present in the
oral cavity.
The most common endodontic filling material is constituted by a combination of a
semi-solid core (gutta-percha) and a fluid sealer [
6
], which have been classically used
following cold gutta-percha techniques (single-cone or lateral condensation) warm com-
paction techniques. In cold obturation techniques, the three-dimensional effectiveness of
the apical seal relies exclusively upon the sealer [
7
,
8
], while in warm techniques a hermetic
apical seal is achieved both owing to the sealer and the heated gutta-percha.
The ideal properties of an effective endodontic sealer were first suggested by Grossman
in 1978 [
9
]. Several sealers have been proposed over time: zinc oxide–eugenol formulations,
calcium hydroxide sealers, glass ionomers, epoxy resin-based, methacrylate resin-based,
and the recently introduced calcium silicate-based sealers [10].
Zinc oxide–eugenol-based sealers are obtained mixing a liquid preparation of eugenol
with a powder containing zinc oxide stably crystallized in a hexagonal wurtzite structure,
as confirmed by X-ray diffraction (XRD) analysis [
11
,
12
]. The setting reaction of zinc
oxide–eugenol is a classical acid–base reaction giving a salt (amorphous chelate of zinc
eugenolate) and water [
13
]. They are widely used in endodontics due to many advantages:
antimicrobial activity [
14
,
15
], resorption in case of extrusion into periradicular tissues [
16
],
and conveniently slow setting time [
17
]. However, they also have some disadvantages:
possible discoloration of tooth structure [
18
], shrinkage on setting [
19
], cytotoxic potential,
Coatings 2021,11, 727. https://doi.org/10.3390/coatings11060727 https://www.mdpi.com/journal/coatings
Coatings 2021,11, 727 2 of 12
and interference with adhesive procedures due to the free eugenol release in tissue fluids
and dental tissues [20,21].
With the recent introduction of calcium silicate-based endodontic sealers, a more
biological approach has taken hold [
22
]. As confirmed by XRD phase observations, these
innovative materials are based on tricalcium silicate, calcium phosphate, zirconium oxide,
and calcium hydroxide [
23
], and hence they are also known as “bioceramics” or “hydraulic
calcium silicate-based sealers” [
24
] or “calcium phosphate-based root canal sealers” [
25
].
Bioceramics raised great interest because of their bioactivity when in contact with tissue
fluids [
22
], their high biocompatibility (with no irritation of periradicular tissues) [
26
],
their slight expansion after setting (which helps to create a hermetic barrier) [
27
], and their
content of calcium phosphate (which allows to create a crystalline structure similar to tooth
and bone apatite materials, improving adhesion to the root dentin) [28].
In some particular situations, such as in presence of widely oval canals or extremely
complex anatomies [
29
], cold gutta-percha techniques have been criticized as they would
hardly allow the complete filling of the whole endodontic space [
30
], while warm obtura-
tion techniques would appear more suitable to achieve a hermetic and three-dimensional
apical seal [
31
]. However, most manufacturers, in agreement with recent literature re-
views [
32
], strongly recommend the use of bioceramics according to the single-cone obtura-
tion technique. Many properties of tricalcium-silicate-based sealers render them particu-
larly suitable to be used in combination with such a technique: they show no shrinkage, no
resorption, and they may undergo some degree of expansion upon setting [
33
]. Several
in vitro
studies [
33
–
39
] investigated the possible improvement of the apical seal quality
by combining tricalcium-silicate-based root canal sealers to the warm vertical compaction,
but leading to contrasting results. Camilleri [36] keeps on recommending the use of these
type of sealers exclusively with cold obturation techniques; Viapiana at al. [
38
], on the
other hand, claimed the absence of obvious differences when using bioceramic sealers with
cold or warm obturation techniques. Thus, further research seems still required to help
clarifying such an ongoing debate.
On the above bases, the aim of the present
in vitro
study is to compare the extent of
methylene blue dye penetration through the apical foramen following endodontic obturation
procedures performed employing a zinc-eugenol and a bioceramic root canal sealer, in
combination with the single-cone and with the continuous wave of
condensation techniques
.
The null hypotheses to be tested were that there were no statistically significant differences
in terms of microleakage between the tested sealers and between the obturation techniques.
2. Materials and Methods
The present
in vitro
research has been conducted in full accordance with the World
Medical Association Declaration of Helsinki and with the rules of the local ethic committee
of “G. D’Annunzio” University of Chieti, Italy. All human biological materials were
obtained during routine dental procedures, with patients’ agreements. All teeth used
for the experimental procedures were extracted for periodontal reasons and they would
have been extracted anyhow, even if not included in the present investigation: they were
not thrown away but were kept with the aim of being employed for the experimental
procedures, with patients’ agreement.
Forty extracted human upper central incisors (n = 40) were selected. All teeth were
inspected with a stereomicroscope 20
×
(MDG17, Wild Heerbrugg, Heerbrugg, Switzerland)
to detect the presence of caries, open apices, fractures, or resorption areas, that represented
exclusion criteria. The specimens were carefully cleansed with ultrasound to remove
extraneous tissue and calculus, and then stored in an aqueous solution of 0.4% chloramine-
T before the use.
The crowns were horizontally sectioned at the canal entrance level using a cylindrical
diamond bur operated with a high-speed electric handpiece (200,000 rpm) and under abun-
dant irrigation, so that the length of all roots was adjusted to approximately
12 mm
. The
work length was determined by a K-file # 15 (Dentsply Maillefer, Ballaigues, Switzerland)
Coatings 2021,11, 727 3 of 12
introduced in the canal space until the tip was just visible at the apical foramen. Then the
roots were cleaned and shaped with Mtwo rotary system files (Sweden & Martina SpA,
Padova, Italy) alternating each instrument with a profuse irrigation of a 5.25% sodium
hypochlorite (NaOCl) solution. All canals were enlarged to size 50, 0.04 taper, to working
length. After preparation, the canals were irrigated with 5 mL 5.25% NaOCl followed
by 5 mL 17% ethylenediaminetetraacetic acid (EDTA) for 60 s to remove the smear layer.
Successively, the specimens were irrigated with distilled water to avoid the prolonged
effect of the EDTA and NaOCl solutions, then the canals were dried with paper points.
Finally, external surfaces of the roots were coated with three layers of clear nail varnish
except for the apical foramen, which was kept patent by introducing a size 25 k-file in the
apex prior to the application of the nail varnish.
The roots were randomly divided into four groups, according to the root canal sealer
and the obturation technique used.
Information about the endodontic sealers investigated in this study (Figure 1) are
summarized in Table 1.
Coatings 2021, 11, x FOR PEER REVIEW 3 of 12
The crowns were horizontally sectioned at the canal entrance level using a cylindrical
diamond bur operated with a high-speed electric handpiece (200,000 rpm) and under
abundant irrigation, so that the length of all roots was adjusted to approximately 12 mm.
The work length was determined by a K-file # 15 (Dentsply Maillefer, Ballaigues, Switzer-
land) introduced in the canal space until the tip was just visible at the apical foramen.
Then the roots were cleaned and shaped with Mtwo rotary system files (Sweden & Mar-
tina SpA, Padova, Italy) alternating each instrument with a profuse irrigation of a 5.25%
sodium hypochlorite (NaOCl) solution. All canals were enlarged to size 50, 0.04 taper, to
working length. After preparation, the canals were irrigated with 5 mL 5.25% NaOCl fol-
lowed by 5 mL 17% ethylenediaminetetraacetic acid (EDTA) for 60 s to remove the smear
layer. Successively, the specimens were irrigated with distilled water to avoid the pro-
longed effect of the EDTA and NaOCl solutions, then the canals were dried with paper
points. Finally, external surfaces of the roots were coated with three layers of clear nail
varnish except for the apical foramen, which was kept patent by introducing a size 25 k-
file in the apex prior to the application of the nail varnish.
The roots were randomly divided into four groups, according to the root canal sealer
and the obturation technique used.
Information about the endodontic sealers investigated in this study (Figure 1) are
summarized in Table 1.
(a)
(b)
Figure 1. Root canal sealers investigated in the study: (a) zinc-oxide-eugenol-based canal sealer; (b)
tricalcium-silicate-based sealer.
Table 1. Information about the root canal sealers investigated in the study.
Type
Product Name (Man-
ufacturer, Country)
Composition
Lot. No
Zinc-oxide-eugenol-based
sealer
Pulp Canal Sealer
(Kerr, Orange, CA,
USA)
Powder
Liquid
Zinc oxide, precipitated silver, oleoresin, thy-
mol iodide
Oil of cloves, Canada balsam
7,929,829
Tricalcium-silicate-based sealer
BioRoot RCS
(Septodont, Saint-
Maur-des-Fossés,
France)
Powder
Liquid
Tricalcium silicate, zirconium oxide
Aqueous solution of calcium chloride
B26389
2.1. Group 1: Zinc-Oxide-Eugenol-Based Canal Sealer and Single-Cone Technique
A single cone of gutta-percha tapered with diameter and conicity corresponding to
the final shaping instrument (# 50.04) was tried in the root canal after visual and tug-back
control. The accuracy of the working-length was assessed by inspecting the tooth through
the apex with a stereomicroscope (20× magnifications) and by periapical radiography. The
canal was dried and the sealer (Pulp Canal Sealer, Kerr, Orange, CA, USA) was mixed
according to the manufacturer’s instructions. Then, the single cone was coated with sealer
and inserted to the working-length with slow movement in order to allow the sealer to
Figure 1.
Root canal sealers investigated in the study: (
a
) zinc-oxide-eugenol-based canal sealer; (
b
) tricalcium-silicate-
based sealer.
Table 1. Information about the root canal sealers investigated in the study.
Type Product Name
(Manufacturer, Country) Composition Lot. No
Zinc-oxide-eugenol-based sealer Pulp Canal Sealer
(Kerr, Orange, CA, USA)
Powder
Liquid
Zinc oxide, precipitated
silver, oleoresin,
thymol iodide
Oil of cloves,
Canada balsam
7,929,829
Tricalcium-silicate-based sealer
BioRoot RCS
(Septodont,
Saint-Maur-des-Fossés, France)
Powder
Liquid
Tricalcium silicate,
zirconium oxide
Aqueous solution of
calcium chloride
B26389
2.1. Group 1: Zinc-Oxide-Eugenol-Based Canal Sealer and Single-Cone Technique
A single cone of gutta-percha tapered with diameter and conicity corresponding to
the final shaping instrument (# 50.04) was tried in the root canal after visual and tug-back
control. The accuracy of the working-length was assessed by inspecting the tooth through
the apex with a stereomicroscope (20
×
magnifications) and by periapical radiography. The
canal was dried and the sealer (Pulp Canal Sealer, Kerr, Orange, CA, USA) was mixed
according to the manufacturer’s instructions. Then, the single cone was coated with sealer
and inserted to the working-length with slow movement in order to allow the sealer to
Coatings 2021,11, 727 4 of 12
flow back coronally and avoid apical extrusion. The coronal excess of gutta-percha was
finally removed with a heated instrument.
2.2. Group 2: Tricalcium-Silicate-Based Sealer and Single-Cone Technique
In this group all the sample preparation procedures were exactly the same as those
described for to the previous group, but a tricalcium-silicate-based sealer (BioRoot RCS,
Septodont SAS, Saint-Maur-des-Fossés, France) was used.
2.3. Group 3: Zinc-Oxide-Eugenol-Based Canal Sealer and Continuous Wave of
Condensation Technique
A master gutta-percha cone tapered with diameter and conicity corresponding to the
final shaping instrument (# 50.04) was selected, then the tip was trimmed with a scalpel
in order to obtain a proper tug-back at 0.5 mm from the working length. The zinc-oxide-
eugenol-based sealer was manipulated with 1:1 proportion according to the manufacturer’s
instructions. After setting the System B (Sybrondental, Orange, CA, USA) temperature at
200
◦
C, a Buchanan plugger was mounted on the device’s handle and inserted at 5 mm
short of the working length without binding on the canal walls; at this level, a rubber
stopper was positioned to mark the coronal reference point. The master cone was then
coated with sealer and fitted into the canal space in order to place the material on the
dentinal walls up to the apical level. Afterwards, the plugger was activated and inserted
into the canal filled with the gutta-percha point until the rubber stopper reached the coronal
reference point. The heat source was deactivated and the plugger was pushed in apical
direction for 10 s with the aim of compensating the gutta-percha shrinkage and allowing
the penetration of the thermoplastic gutta-percha (and eventually of the sealer) in the
accessory canals. The power of the heat source was activated again for 1 s to separate the
plugger from the compacted gutta-percha; then the plugger was extracted and a manual
plugger was immediately inserted to verify the presence of the core material at the apical
level and its adequate compaction [
40
]. Back-filling of the rest of the canal space was
achieved by injecting (Obtura II; Obtura Corporation, Fenton, MO, USA) and compacting
warm gutta-percha with a pre-fitted plugger.
2.4. Group 4: Tricalcium-Silicate-Based Sealer and Continuous Wave of Condensation Technique
In this group the root canal obturation was achieved following the technique described
for Group 3, but the same tricalcium-silicate-based root canal sealer used in Group 2
was employed.
Specimens from every group were stored at 37
◦
C and 100% humidity for 72 h, prior
to be subjected to the subsequent experimental procedures.
After completing the isolation of the root by covering the coronal surface with three
additional layers of nail varnish, the teeth were immersed in an aqueous solution of 2%
methylene blue dye and stored in an incubator, at 37
◦
C for 72 h. Following exposure to
dye, the roots were meticulously rinsed under running water.
The samples were included in self-curing acrylic resin and then put on a coverslip.
The experimental teeth were sectioned longitudinally using a low-speed diamond saw
(Micromet M; Remet S.p.A., Casalecchio di Reno, Italy) in a direction approximately
parallel to the long axis of the tooth and through the apex in order to expose the canal
space. Sectioned samples were placed on a piece of millimetric paper and examined under
a stereomicroscope at 20
×
magnifications. Images were captured and then saved using
a digital device. To estimate the microleakage, the images were imported in a specific
software (Image J version 1.42q; National Institutes of Health, Bethesda, MD, USA) and
analyzed after setting a scale using the millimetric paper on the background of every
sample. The calculation of the microleakage was made by measuring the distance (mm)
between the apical foramen and the most coronal point of dye infiltration.
Means and standard deviations were calculated for each experimental group. The
differences between the means were statistically analyzed by two-way analysis of vari-
ance (ANOVA), in order to assess the effect of the two factors under investigation (the
Coatings 2021,11, 727 5 of 12
“endodontic sealer” and the “obturation technique”) on the microleakage mean values.
Multiple comparisons were performed by a Tukey Test. The level of significance was set at
p< 0.05 in all tests.
After microleakage evaluation, representative samples from every experimental group
were dehydrated in progressive concentrations of ethyl alcohol (70%, 80%, 90%, and 100%)
and sputter-coated with a 300-A
◦
-thick gold layer. They were then observed under a
scanning electron microscope (SEM) (EVO 50 XVP LaB6; Carl Zeiss SMT Ltd., Cambridge,
UK) at 1000
×
and 3500
×
magnification, in order to assess the potential sealer penetration
into the dentinal tubules.
3. Results
The obtained microleakage mean values (and standard deviations), organized accord-
ing to the factors under investigation (endodontic sealer and obturation technique), are
shown in Table 2and graphically depicted in Figure 2.
Table 2.
Mean values (mm) and standard deviations (mm) of the microleakage in each experimental
group organized according to the considered factors: endodontic sealer and obturation technique.
Endodontic Sealer
Obturation Technique
Single Cone Continuous Wave
Zinc-oxide-eugenol-based
sealer (ZOE)
Group 1
3.63 1a(0.82)
Group 3
2.46 2a(1.29)
Tricalcium-silicate-based
sealer (BioRoot)
Group 2
2.33 1b(0.85)
Group 4
1.02 2b(0.60)
a,b
Different superscript letters suggest statistically significant differences in vertical comparisons.
1,2
Different
numbers in pedex suggest statistically significant differences in horizontal comparisons.
Coatings 2021, 11, x FOR PEER REVIEW 5 of 12
Means and standard deviations were calculated for each experimental group. The
differences between the means were statistically analyzed by two-way analysis of vari-
ance (ANOVA), in order to assess the effect of the two factors under investigation (the
“endodontic sealer” and the “obturation technique”) on the microleakage mean values.
Multiple comparisons were performed by a Tukey Test. The level of significance was set
at p < 0.05 in all tests.
After microleakage evaluation, representative samples from every experimental
group were dehydrated in progressive concentrations of ethyl alcohol (70%, 80%, 90%,
and 100%) and sputter-coated with a 300-A°-thick gold layer. They were then observed
under a scanning electron microscope (SEM) (EVO 50 XVP LaB6; Carl Zeiss SMT Ltd.,
Cambridge, UK) at 1000× and 3500× magnification, in order to assess the potential sealer
penetration into the dentinal tubules.
3. Results
The obtained microleakage mean values (and standard deviations), organized ac-
cording to the factors under investigation (endodontic sealer and obturation technique),
are shown in Table 2 and graphically depicted in Figure 2.
Table 2. Mean values (mm) and standard deviations (mm) of the microleakage in each experimental
group organized according to the considered factors: endodontic sealer and obturation technique.
Endodontic Sealer
Obturation Technique
Single Cone
Continuous Wave
Zinc-oxide-eugenol-based sealer (ZOE)
Group 1
3.63 1 a (0.82)
Group 3
2.46 2 a (1.29)
Tricalcium-silicate-based sealer (BioRoot)
Group 2
2.33 1 b (0.85)
Group 4
1.02 2 b (0.60)
a,b Different superscript letters suggest statistically significant differences in vertical comparisons.
1,2 Different numbers in pedex suggest statistically significant differences in horizontal compari-
sons.
Figure 2. Apical microleakage mean values (mm) and standard deviations (mm) of the studied sam-
ples, organized according to the considered factors (endodontic sealer and obturation technique).
3.63 mm
2.46 mm 2.33 mm
1.02 mm
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
GRUPPO
ZoE-SingleCone ZoE-ContinuousWave
BioRoot-SingleCone BioRoot-ContinuousWave
Figure 2.
Apical microleakage mean values (mm) and standard deviations (mm) of the studied
samples, organized according to the considered factors (endodontic sealer and obturation technique).
The maximum infiltration values (3.63 mm) were observed in Group 1 (zinc-oxide-
eugenol-based sealer and single cone technique), significantly higher compared to all the
other experimental groups.
Coatings 2021,11, 727 6 of 12
The lowest apical microleakage mean values (1.02 mm) were found in Group 4
(tricalcium-silicate-based sealer and the continuous wave of condensation technique),
significantly reduced compared to the remaining experimental conditions tested.
No statistically significant differences were observed when comparing Group 2
(tricalcium-silicate-based sealer and single-cone technique) and Group 3 (zinc-oxide-
eugenol-based canal sealer and continuous wave of condensation technique).
SEM analysis showed an evident penetration of the bioceramic root canal sealer into
the dentinal tubules (Figure 3), whereas in samples obturated with zinc-oxide-eugenol-
based sealer dentinal tubules appeared empty (Figure 4).
Coatings 2021, 11, x FOR PEER REVIEW 6 of 12
The maximum infiltration values (3.63 mm) were observed in Group 1 (zinc-oxide-
eugenol-based sealer and single cone technique), significantly higher compared to all the
other experimental groups.
The lowest apical microleakage mean values (1.02 mm) were found in Group 4 (trical-
cium-silicate-based sealer and the continuous wave of condensation technique), signifi-
cantly reduced compared to the remaining experimental conditions tested.
No statistically significant differences were observed when comparing Group 2
(tricalcium-silicate-based sealer and single-cone technique) and Group 3 (zinc-oxide-eu-
genol-based canal sealer and continuous wave of condensation technique).
SEM analysis showed an evident penetration of the bioceramic root canal sealer into
the dentinal tubules (Figure 3), whereas in samples obturated with zinc-oxide-eugenol-
based sealer dentinal tubules appeared empty (Figure 4).
Figure 3. Scanning electron microphotograph ((A), original magnification 1000×; (B), original magnification 3500×) of a
sample obturated with a bioceramic-based root canal sealer and warm vertical condensation. Sealer penetration into the
dentinal tubules may be observed (arrows).
Figure 4. Scanning electron microphotograph ((A), original magnification 1000×; (B), original magnification 3500×) of a
sample obturated with zinc-oxide-eugenol-based root canal sealer and warm vertical condensation. Dentinal tubules ap-
pear empty.
4. Discussion
The null hypotheses tested in the present study had to be rejected: there were statis-
tically significant differences in terms of microleakage between the tested sealers and ob-
turation techniques.
Figure 3.
Scanning electron microphotograph ((
A
), original magnification 1000
×
; (
B
), original magnification 3500
×
) of a
sample obturated with a bioceramic-based root canal sealer and warm vertical condensation. Sealer penetration into the
dentinal tubules may be observed (arrows).
Coatings 2021, 11, x FOR PEER REVIEW 6 of 12
The maximum infiltration values (3.63 mm) were observed in Group 1 (zinc-oxide-
eugenol-based sealer and single cone technique), significantly higher compared to all the
other experimental groups.
The lowest apical microleakage mean values (1.02 mm) were found in Group 4 (trical-
cium-silicate-based sealer and the continuous wave of condensation technique), signifi-
cantly reduced compared to the remaining experimental conditions tested.
No statistically significant differences were observed when comparing Group 2
(tricalcium-silicate-based sealer and single-cone technique) and Group 3 (zinc-oxide-eu-
genol-based canal sealer and continuous wave of condensation technique).
SEM analysis showed an evident penetration of the bioceramic root canal sealer into
the dentinal tubules (Figure 3), whereas in samples obturated with zinc-oxide-eugenol-
based sealer dentinal tubules appeared empty (Figure 4).
Figure 3. Scanning electron microphotograph ((A), original magnification 1000×; (B), original magnification 3500×) of a
sample obturated with a bioceramic-based root canal sealer and warm vertical condensation. Sealer penetration into the
dentinal tubules may be observed (arrows).
Figure 4. Scanning electron microphotograph ((A), original magnification 1000×; (B), original magnification 3500×) of a
sample obturated with zinc-oxide-eugenol-based root canal sealer and warm vertical condensation. Dentinal tubules ap-
pear empty.
4. Discussion
The null hypotheses tested in the present study had to be rejected: there were statis-
tically significant differences in terms of microleakage between the tested sealers and ob-
turation techniques.
Figure 4.
Scanning electron microphotograph ((
A
), original magnification 1000
×
; (
B
), original magnification 3500
×
) of
a sample obturated with zinc-oxide-eugenol-based root canal sealer and warm vertical condensation. Dentinal tubules
appear empty.
4. Discussion
The null hypotheses tested in the present study had to be rejected: there were sta-
tistically significant differences in terms of microleakage between the tested sealers and
obturation techniques.
Coatings 2021,11, 727 7 of 12
Several methods have been proposed in the literature to investigate microleakage:
Fluid filtration or transportation method, which consists in measuring an air bubble move-
ment within a capillary tube [
41
,
42
]; dye extraction or dissolution method, in which the
teeth are dissolved in acids to release the dye from the interfacial areas and obtain a solution
whose optical density is determined with a spectrophotometer [
41
]; bacteria and toxin
infiltration method, a qualitative evaluation whereby penetration of bacterial organisms is
estimated [
43
]; glucose penetration model, which relies on the assessment of the filtration
rate of glucose along the root canal obturation material [
44
,
45
]; protein microleakage test,
that is performed using bovine serum albumin in a dual-chamber apparatus and estimated
using a spectrophotometer [
46
,
47
]; electrochemical microleakage method, which consists
in the diffusion of ions through narrow spaces [
48
]; three-dimensional methods carried out
using cone-beam computed tomography (CBCT) [
49
] and SEM evaluations [
50
]; dye pene-
tration methods, in which teeth are immersed in various types of dyes and then sectioned
(longitudinally or transversely) or cleared to record linear penetration of the dye [
43
,
51
]. To
date, there is still some debate about the most reliable method for investigating the apical
microleakage in endodontics. In this study, the dye penetration method was preferred
because of its relative simplicity and easy replicability.
In the present research, the highest dye infiltration values were observed in samples
from Group 1, probably because they combined the intrinsic limits of both a conventional
sealer and a cold compaction technique. Among the main disadvantages of a single-cone
technique, the lack of a sufficiently homogeneous obturation has been often underlined,
together with the reduced chance of filling every void, which ultimately provide an apical
seal whose predictability ends up relying just on the specific properties of the endodontic
sealer. Clinically, this could represent a limit in case of resorbable sealers [
7
,
8
]. In Group 1, a
conventional zinc-oxide-eugenol-based canal sealer was used: based on previous research,
such a material shows a certain degree of shrinkage after setting [
19
], which might lead
to the generation of voids. In Group 2, a tricalcium-silicate-based sealer was used in
combination with the single-cone technique and the dye penetration turned out to be
significantly lower than in Group 1. Tricalcium-silicate sealers have the ability to undergo
a slight expansion after setting [
27
], which probably helped increasing the tightness of the
apical seal even if combined with a particularly inefficient obturation strategy. The present
results would support the wide-spread habit of employing bioceramic sealers together
with a single cold gutta-percha cone [
52
], as the many limitations of such an extremely
simplified obturation technique seem to be overcome owing to the advantageous properties
of those innovative materials.
Tricalcium-silicate-based sealers seem to positively interact with dentin fluid, poten-
tially inducing biomineralization with the formation of mineral tags within the dentinal
tubules, thus enhancing the biological activity within the root canal [
53
], due to the pres-
ence of calcium phosphate in their composition that enhances the setting mechanism [
28
]
and improves the chemical bonding between the sealer and the root dentin [
26
]. Calcium
phosphate is also the main reason for the outstanding bioceramic’s biocompatibility, that
is their ability to not trigger adverse reaction when in contact with tissues [
28
]. Calcium
phosphate is, in fact, one of the major inorganic constituents of hard tissues. Indeed, it
has been shown that these materials can even promote bone regeneration [
54
] when acci-
dentally extruded through the apical foramen during root canal filling procedures [
55
,
56
].
These materials have also the ability to establish a micromechanical retention by tag sealer
penetration in dentinal tubules [
57
]. In the present study, the scanning electron microscope
analysis confirmed a visible penetration of the bioceramic root canal sealer into the denti-
nal tubules: this behavior may help establishing a stronger physical barrier, improving
retention of the root filling, entombing possible residual bacteria into dentinal tubules
and, probably, enhancing the ultimate quality of the apical seal [
35
]. Among the other
interesting properties of bioceramic-based sealers, the following should be underlined:
An adequate setting time [
26
,
58
,
59
]; a reduced risk of discoloration of tooth structures
when compared with other conventional root canal sealers [
26
]; a high radiopacity due to
Coatings 2021,11, 727 8 of 12
the presence of bismuth trioxide [
60
,
61
]; an antimicrobial activity, owing to the high pH
values and the high tendency to release calcium ions [
62
,
63
]. Of course, these materials
are not completely free from possible shortcomings: a major drawback is their complex
removal in case of orthograde endodontic retreatments [
64
]. Moreover, endodontically
treated teeth may often benefit from the use of adhesively luted fiber-post to effectively
support the coronal restoration [
65
–
70
] but a thorough cleaning of the root canal walls,
which is required for an appropriate post-space preparation, might become definitely
challenging when a bioceramic sealer is used [
64
]. Finally, some doubts seem to come from
a few studies that showed how certain properties of bioceramics, such as flow [
62
,
71
,
72
] or
solubility [73,74], do not steadily fulfil the ISO standard requirements.
In Group 3, the zinc-oxide-eugenol-based canal sealer was used together with the
continuous wave of condensation technique and the microleakage results were comparable
to Group 2, again significantly enhanced compared to Group 1. In this case, the improved
performances can be explained owing to the advantages of the continuous wave of conden-
sation, which typically lead to a denser and more homogeneous filling and allow a better
sealing [
7
,
75
], in presence of less voids, when compared to cold obturation techniques [
76
].
The use of warm gutta-percha allows to fill even the endodontic areas of challenging access,
due to the reduced viscosity and owing to the vertical compaction loads.
In Group 4 (tricalcium-silicate-based sealer and continuous wave of condensation
technique), the dye penetration values were the lowest among all the experimental groups.
According to these authors, such a finding could be explained taking into account the
combined benefits coming from both the warm obturation technique and the advantageous
properties of the bioceramic-based sealer.
5. Conclusions
As previously mentioned, the current literature is not unanimous when dealing with
the association of a bioceramic-based sealer to a warm obturation techniques. Qu et al. [
37
]
showed that after heat application, bioceramic-based root canal sealer exhibited a reduction
in setting time and flow, which could negatively influence the performance of obturation
and the procedure outcome. Camilleri et al. [
36
] observed how the material chemistry
and the physical properties were not affected by the heat, but still suggested to follow the
guidelines of the manufacturers, which recommend the use of a single cone technique.
Other studies [
33
–
35
] affirmed that the obturation technique does not affect the efficacy
of the bioceramic sealers. Hadis et al. [
39
] compared a new bioceramic-based root canal
sealer, designed to be used with a warm obturation technique, with one recommended
for the single cone technique; the results showed no differences between the two sealers,
when they were used following a warm filling technique. Viapiana et al. [
38
] observed
bioceramic sealers to be stable even when used with a warm vertical compaction, but
they also underlined the need for a better investigation about the effect of heat on sealer
properties. Atmeh et al. [
77
] claimed that even if heat does not cause changes in the
bioceramic chemical structure, it seems to produce some microstructural changes due to
water loss. Specifically, a Fourier-transformed infrared spectroscopy (FT-IR) analysis of an
heated bioceramic sealer revealed a drop in the peaks representing vibrational modes of
the OH group in water, thus proving an irreversible water loss above 100
◦
C by means of
evaporation [78].
Within the limits of the present study, it seems that in terms of microleakage of recently
sealed samples a warm obturation technique is promising even when used in association
with a tricalcium-silicate root canal sealer. However, it must be underlined that no attempt
was made herein to artificially simulate any effect of specimen aging. Thus, the long-
term stability of the apical seal obtained with tricalcium-silicate-based sealers subjected
to heat sources should be still verified, and it could represent an interesting subject for
further research.
Based on the results of the present study, the following conclusions can be drawn:
Coatings 2021,11, 727 9 of 12
•
The obturation technique is a factor able to significantly affect the quality of the apical
seal in terms of microleakage and the dye penetration is reduced with a warm wave
of condensation technique, compared to a cold single-cone obturation technique;
•
Obturation technique being equal, tricalcium-silicate-based root canal sealers appear
to be more performing than conventional zinc-eugenol-based sealers;
•
In terms of microleakage, warm obturation techniques seem promising even when
used in combination with a tricalcium-silicate-based root canal sealer.
Author Contributions:
Conceptualization, C.D., F.D.A. and M.V.; data curation, C.D., F.D.A. and
M.V.; formal analysis, F.D.A.; investigation, F.D.A., M.B. and R.A.; methodology, F.D.A. and M.B.;
project administration, C.D. and M.V.; resources, F.D.A. and C.D.; supervision, F.D.A., C.D. and
M.V.; validation, C.D.; visualization, F.D.A. and M.B.; writing—original draft, F.D.A., M.B. and R.A.;
writing—review and editing, F.D.A., M.B. and R.A. All authors have read and agreed to the published
version of the manuscript.
Funding: This research received no external funding.
Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Data Availability Statement:
The data presented in this study are available on request from the
corresponding author.
Conflicts of Interest: The authors declare no conflict of interest.
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