First Report of Octospora neerlandica from Asian Continent

Abstract

The bryophillic ascomycete species, Octospora neerlandica Benkert & Brouwer, is reported as a new record from Turkey, based on the identification of the samples collected from Niğde province. A brief description and photographs, related to the macroscopy and microscopy of the species, are provided.

Full text

MANTAR DERGİSİ/The Journal of Fungus Nisan(2021)12(1)61-64
61
Geliş(Recevied)
:10.12.2020
Research Article
Kabul(Accepted)
:28.01.2021
Doi: 10.30708.mantar.838701
First Report of Octospora neerlandica from Asian Continent
Osman BERBER1, Yasin UZUN2*
Abdullah KAYA3
*Sorumlu yazar: yuclathrus@gmail.com
1Karaman Provincial Directorate of Agriculture and Forestry, 70100, Karaman, Turkey
Orcid ID: 0000-0002-0265-4441 / ober70@hotmail.com
2Karamanoğlu Mehmetbey University, Ermenek Uysal & Hasan Kalan Health Services Vocational
School, 70400, Karaman, Turkey
Orcid ID:0000-0002-6423-6085 / yuclathrus@gmail.com
3Gazi University, Science Faculty, Department of Biology, 06560 Ankara, Turkey
Orcid ID: 0000-0002-4654-1406 / kayaabd@hotmail.com
Abstract: The bryophillic ascomycete species, Octospora neerlandica Benkert & Brouwer,
is reported as a new record from Turkey, based on the identification of the samples collected from
Niğde province. A brief description and photographs, related to the macroscopy and microscopy
of the species, are provided.
Key words: Biodiversity, bryophillic ascomycete, new record, Pyronemataceae, Turkey
Octospora neerlandica'nın Asya Kıtasından İlk Kaydı
Öz: Briyofilik askomiset türü olan, Octospora neerlandica Benkert & Brouwer, Niğde’den
toplanan örneklerin teşhis edilmesiyle, Türkiye’den yeni kayıt olarak rapor edilmiştir. Türün kısa
bir betimlemesi ve makroskobi ve mikroskobisine ilişkin fotoğrafları verilmiştir.
Anahtar kelimeler: Biyoçeşitlilik, briyofilik askomiset, yeni kayıt, Pyronemataceae, Türkiye
Introduction
Octospora Hedw is an ascomycete genus within
the family Pyronemataceae Corda. Moss associated
apothecia, typical marginal hyphae, and ellipsoid to
globose or rounded, sometimes ornamented, guttulate
spores generally characterize the genus (Yao and
Spooner, 1996). Kirk et al. (2008) reports the presence of
84 species of the genus, eighty one of which also exist in
Europe (Benkert, 2007).
Octospora itzerottii Benkert and O. leucoloma
Hedw. were the first two Octospora species reported in
Turkey (Çolak and Kaygusuz, 2017; Uzun et al., 2017). In
the following two years, eleven members of the genus, O.
areolata (Seaver) Caillet & Moyne, O. axillaris (Nees)
M.M. Moser, O. coccinea (P. Crouan & H. Crouan)
Brumm., O. excipulata (Clem.) Benkert, O. gemmicola
Benkert, O. grimmiae Dennis & Itzerott, O. lilacina
(Seaver) Svrček & Kubička, O. musci-muralis Graddon,
O. orthotrichi (Cooke & Ellis) K.B. Khare & V.P. Tewari,
O. polytrichi (Schumach.) Caillet & Moyne and O. rustica
(Velen.) J.Moravec were also presented as new records
for the mycobiota of Turkey by Uzun et al. (2018) and
Uzun and Kaya (2019). But the current checklists (Sesli
and Denchev, 2014; Solak et al., 2015) on Turkish
macromycota and the later contributions (Alkan et al.,
2018; Doğan et al., 2018; Işık and Türkekul, 2018; Acar
et al., 2019; Allı et al., 2019; Berber et al., 2019; Keleş,
2019; Şelem et al., 2019; Türkekul and Işık, 2019; Akçay,
2020; Çağli and Öztürk, 2020; Çelik et al., 2020; İleri et
al., 2020; Sadullahoğlu and Uzun, 2020; Sesli, 2020;
Uzun et al., 2020), indicate that, O. neerlandica hasn’t
been reported from Turkey before.
The study aims to make a contribution to the
mycobiota of Turkey.
Material and method
The fruit body of O. neerlandica was collected from
Ulukışla district of Niğde province, in 2018, during a
routine field trip performed to determine the macrofungal
biodiversity of the district. The sample was photographed
at its natural habitat, and ecological characteristics and
geographic position were noted. Then it was put in a

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paper box and transferred to the fungarium where it was
dried and prepared as fungarium material. Microscopic
investigations were carried out on dry samples. A Nikon
Eclipse Ci-S trinocular microscope was used for
microscopic investigation and a DS-Fi2 digital camera
was used to get microstructural photographs. The sample
was identified with the help of Benkert and Brouwer
(2004). The specimen is kept at Karamanoğlu
Mehmetbey University, Kâmil Özdağ Science Faculty,
Department of Biology.
Results
Ascomycota Caval-Sm
Pezizomycetes O.E.Erikss. & Winka
Pezizales J.Schröt.
Pyronemataceae Corda
Octospora neerlandica Benkert & Brouwer,
Persoonia 18(3): 381 (2004)
Macroscopic and microscopic features:
Apothecium 1.8 mm, margin fimbriate, membranaceous,
hymenial surface pinkish-orange to pale orange (Figure
1a), outside portion somewhat paler. Asci 210-290  13-
18 μm, cylindrical, 8-spored, spores uniseriate
Paraphyses slender, generally straight, some slightly
curved, enlarged up to 3-7 μm, at the apex (Figure 1b).
Ascopores 16-18  11-12.8 μm, ellipsoid, one oil drop
generally accompained by smaller droplets, spore
ornamentation constists of an irregular reticulum with
variably formed meshes (Figure 1c).
Figure 1. Ascocarp (a), asci and paraphyses (b) and ascospores (c) of Octospora neerlandica (bars: a- 1 mm, b,c- 10 μm)

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Octospora neerlandica was reported to grow on
sandy soil among or directly on the the stems of the
mosses Tortula ruralis (Hedw.) Gaertn., Meyer, &
Scherb., T. ruraliformis (Besch.) Ingham or T. virescens
(De Not.) De Not. (Benkert and Brouwer, 2004).
Specimen examined: Turkey, Niğde, Ulukışla,
Çiftehan village, on soil among mosses under Pinus sp.,
37°30′N, 34°47′E, 930 m, 07.01.2018, O.Ber-108.
Discussion
Octospora neerlandica is reported for the first time
for the mycobiota of Turkey. It is the 14th member of the
genus Octospora in Turkey (Çolak and Kaygusuz, 2017;
Uzun et al., 2017, 2018; Uzun and Kaya, 2019).
Macroscopic, microscopic and habitat characteristics of
Turkish collection are in agreement with Benkert and
Brouwer (2004).
Due to their morphological similarities and very
small size, Octospora species can easily be overlooked
and it is very hard to separate their species from each
other morphologically (Itzerott, 1977). But the spore
characteristics and substrate preferation of O.
neerlandica makes it a very distinctive species. The spore
ornamentation of this species constists of an irregular
reticulum with variably formed meshes. Though
Lamprospora seaveri Benkert has a remarkable similar
spore ornamentation, it has globose spores and is
associated with other moss species. Likewise very little is
known about the infection of the memsers of the moss
genus Tortula Hedw. by Octospora species. Although O.
crosslandii (Dennis & Itzerott) Benkert, has been
observed in association with Tortula species together with
many other moss species, the globose spores of this
species easily differentiates it from O. neerlandica
(Benkert and Brouwer, 2004).
References
Acar, İ., Uzun, Y., Keleş, A. and Dizkırıcı Tekpınar, A. (2019). Suillellus amygdalinus, a new species record for Turkey from
Hakkari Province. Anatolian Journal of Botany, 3(1): 25-27.
Akçay, M.E. (2020). A new record for the Mycota of Turkey. Anatolian Journal of Botany, 4(1): 8-10.
Alkan, S., Aktaş, S. and Kaşık, G. (2018). Tuber species in Turkey and a new locality for Tuber aestivum. Selcuk University
Journal of Science Faculty, 44(1): 25-29.
Allı, H., Tevlim, G. and Şen, İ. (2019). A new record for Turkey's mycobiota from an interesting habitat in the Muğla province:
Hortiboletus bubalinus (Oolbekk. & Duin) L. Albert & Dima. Muğla Journal of Science and Technology, 5(1): 114-118.
Benkert, D. (2007). Zur Kenntnis des Vorkommens bryophiler Pezizales (Ascomycota) in Südost-Europa. Mycologia
Montenegrina, 10: 7-21.
Benkert, D. and Brouwer, E. (2004). New species of Octospora and some further remarkable bryoparasitic Pezizales from
the Netherlands. Persoonia, 18(3): 381-391.
Berber, O., Uzun, Y. and Kaya, A. (2019). Genea lobulata, a new hypogeous ascomycete record for Turkish Mycobiota.
Süleyman Demirel University Journal of Natural and Applied Sciences, 23(3): 922-924.
Çağli, G. and Öztürk, A. (2020). Macromycetes determined in Muradiye (Van) district. Anatolian Journal of Botany, 4(1):
57-64.
Çelik, A., Uzun, Y. and Kaya, A. (2020). Macrofungal biodiversity of Güneysınır district (Konya-Turkey). The Journal of
Fungus, 11(1): 75-83.
Çolak, Ö.F. and Kaygusuz, O. (2017). Octospora leucoloma (Pyronemataceae): a new bryoparasitic genus record for
Turkish mycobiota. Phytologia Balcanica, 23(3): 345-348.
Doğan, H.H., Bozok, F. and Taşkın, H. (2018). A new species of Barssia (Ascomycota, Helvellaceae) from Turkey. Turkish
Journal of Botany, 42(5): 636-643.
Işık, H. and Türkekul, İ. (2018). New additions to Turkish macrofungi from Tokat and Yozgat Provinces. Mycotaxon, 133(4):
697-709.
Itzerott, H. (1977). The genus Octospora and an attempt to solve its taxonomic problems. Kew Bulletion, 31(3): 497-500.
İleri, R., Uzun, Y. and Kaya, A. (2020). Macromycetes of Karadağ (Karaman) and its environs. The Journal of Fungus,
11(1): 57-63.
Keleş, A. (2019). New records of macrofungi from Trabzon province (Turkey). Applied Ecology and Environmental
Research, 17(1): 1061-1069.
Kirk, P.M., Cannon, P.F., Minter, D.W. and Stalpers, J.A. (2008). Dictionary of the Fungi, 10th ed. CAB International,
Wallingford.
Sadullahoğlu, C. and Uzun, Y. (2020). Karz Dağı (Tatvan-Bitlis) ve çevresinde belirlenen makrofunguslar. Mantar Dergisi,
11(1): 1-11.
Sesli, E. (2020). Clitopilus cystidiatus (Entolomataceae), Türkiye Mikotası için yeni bir kayıt. Mantar Dergisi, 11(1): 90-93.

MANTAR DERGİSİ/The Journal of Fungus Nisan(2021)12(1)61-64
64
Sesli, E. and Denchev, C.M. (2014). Checklists of the myxomycetes, larger ascomycetes, and larger basidiomycetes in
Turkey. 6th edn. Mycotaxon Checklists Online (http://www.mycotaxon.com/resources/checklists/sesli-v106-
checklist.pdf): 1-136.
Solak, M.H., Işıloğlu, M., Kalmış, E. and Allı, H. (2015). Macrofungi of Turkey, Checklist. Volume- II. Üniversiteliler Ofset,
İzmir.
Şelem, E., Keleş, A., Acar, İ. and Demirel, K. (2019). Edible macrofungi determined in Gürpınar (Van) district. Anatolian
Journal of Botany, 3(1): 7-12.
Türkekul, İ. and Işık, H. (2019). Macrofungal biodiversity of Reşadiye (Tokat) district. Acta Biologica Turcica, 32(2): 95-101.
Uzun, Y. and Kaya, A. (2019). New Additions to Turkish Pezizales from East Blacksea Region. Turkish Journal of Botany,
43(2): 262-270.
Uzun, Y., Acar, İ., Akçay, M.E. and Sadullahoğlu, C. (2020). Kağızman (Kars) yöresi makrofungusları. Mantar Dergisi, 11(1):
19-28.
Uzun, Y., Karacan, İ.H., Yakar, S. and Kaya, A. (2017). Octospora Hedw., A New Genus Record for Turkish
Pyronemataceae. Anatolian Journal of Botany, 1(1): 18-20.
Uzun, Y., Karacan, İ.H., Yakar, S. and Kaya, A. (2018). New bryophillic Pyronemataceae records for Turkish Pezizales from
Gaziantep province. Anatolian Journal of Botany, 2(1): 28-38.
Yao, Y.J. and Spooner, B.M. (1996). Notes on British species of Octospora. Mycological Research, 100: 175-178.