Coupled effects of photoperiod, temperature and salinity on diapause induction were experimentally studied using a diploid clone of the parthenogenetic Artemia from Barkol Lake, Xinjiang, China. We conducted three experiments, each containing nine treatments (combinations of two of the above three factors, each with three levels). When photoperiod was set at 12L(light):12D(dark), combinations of ... [Show full abstract] salinity 70/140 ppt and temperature 19/23°C led most females to produce diapaused offspring (resting eggs / cysts; percentage of diapaused broods being 81.60±32.51% [mean±SD; same below] to 99.28±3.48%). Under combinations of salinity 70/140 ppt and 27°C most broods were nauplii (percentage of diapaused broods being 28.46±30.68% to 38.43±23.22%). When salinity was set to 70 ppt, treatment conditions of 19/23/27°C + 6L:18D and 19/23°C + 12L:12D induced high percentage of diapaused broods (72.11±36.42% to 100±0.00%). When temperature was set to 25°C, overwhelming cyst production was only observed under 6L:18D + 70/140 ppt (percentage of diapaused broods being 83.33±23.57% to 93.86±14.10%). These results suggest that the modes of offspring production in the studied Artemia clone are strongly influenced by photoperiod and temperature and there is significant interaction between the two factors. Lower temperature and shorter daylight tend to induce production of resting eggs, and the effect of photoperiod may dominate over the effect of temperature. Though an interaction between salinity and photoperiod was detected, a sound tendency for the salinity effect could not be determined.