ArticlePDF Available
Techno-Science Research Journal 8 (2020) 64-68
Content list available at ITC
Techno-Science Research Journal
Journal Homepage: www.ric.itc.edu.kh
64
Market Study of Soy Sauces in Cambodia
Luka Ly1, Monychot Tepy Chanto2,3, Chanthol Peng2,3, Reasmey Tan2,3*
1 Graduate School, Institute of Technology of Cambodia, Russian Federation Blvd., P.O. Box 86, Phnom Penh, Cambodia
2 Food Technology and Nutrition Research Unit, Research and Innovation Center, Institute of Technology of Cambodia,
Russian Federation Blvd., P.O. Box 86, Phnom Penh, Cambodia
3 Faculty of Chemical and Food Engineering, Institute of Technology of Cambodia, Russian Federation Blvd.,
P.O. Box 86, Phnom Penh, Cambodia
Received: 29 June 2020; Accepted: 08 November 2020; Available online: xxxx
Abstract: Soy sauce is a liquid condiment originated from China and is widely used not only in China, but also in Asia including
Cambodia. Although some soy sauces have been produced in Cambodia, their quality has not yet been widely recognized in Cambodia and
the study of soy sauces in Cambodia is still limited. The objective of this survey aimed to collect information about the current status on soy
sauce consumption in Cambodia, for future research and development of Cambodian soy sauce. Survey’s participants were selected from the
age of 11 years old and up. Each participant was asked to answer all 12 questions before submitting their answers. As a result, among 655
respondents, the average of household size was 4.49 people.Approximately 53.4% of participants consume less than one bottle per month
with their family and 41.9% of consumers used soy sauce between one and three bottles of soy sauce with the daily consumption in this group
being about 4.6 mL per day. Salty-sweet soy sauce is the most preferable taste followed by the slightly salty taste. The volumes of the their
favorite soy sauce bottle were 450 mL, 500mL and 250 mL. Out of 50 soy sauce brands, the soy sauces that had the code C2 (Cambodia’s soy
sauce), V3 (Vietnam’s soy sauce), TH5 and TH3 (Thailand’s soy sauce), are the brands that many respondents in the survey have used, and
83.80% of respondents have consumed the soy sauce that costs between 2500 riels and 7900 riels. In conclusion, the soy sauce brands (C2,
V3, TH5 and TH3) are the brands that are consumed by most of the respondents. Among them, TH5 has higher quality by comparing with the
Cambdodian soy sauce standard. Salty sweet taste is the favorite taste by the respondents.
Keywords: Soy sauce; Survey; Cambodia; Brand; Taste
1. INTRODUCTION1
Soy sauce has been a liquid condiment originated from
China since approximately 2200 years ago. It is produced by
mixing the steamed soybeans with roasted and grided wheat,
and Koji mold spores and incubate for 3 to 5 days followed
by fermentation in brine for about 3 to 6 months (Leboffe
and Pierce, 2006). People in Asian countries such as China,
Japan, Korea, Thailand, the Philippines, Indonesia, and more
than 90% of Cambodian people consumed soy sauce
(Theary et al., 2013). Soy sauce is normally used in cooking,
such as in soups, frying, roasting, marinating, and also for
dipping. It is widely used in the purpose of food, for
improving the taste, flavor and aroma, as it is the main
source of natural umami taste (Marcus, 2019). Not only that,
scientists found that soy sauce also contains bioactive
compounds with anticarcinogenic, antimicrobial,
* Corresponding author: Reasmey Tan
E-mail: rtan@itc.edu.kh; Tel: +855-12-602-202
antioxidative, and antiplatelate activities (Murooka and
Yamshita, 2008) and also antiallergernic activity
(Kobayashi, 2005). The soy sauce consumption and variety
in Cambodia is unknown. Moreover, the quality and safety
of the soy sauces has limited study. The objective of this
market study of soy sauces in Cambodia was to collect
information on the current status on soy sauce consumption
in Cambodia for future research and development of
Cambodian fermented soy sauce.
2. METHODOLOGY
2.1 Survey
The online survey was conducted from 01 February
2020 until 15 June 2020 (using Google forms). Aiming on
participants whose age are from 11 years old and up. The
survey link was sent through Facebook, e-mail, asking
consumers directly, or by telephone calls for collecting the
data of current status on soy sauce consumption in
Ly et al./Techno-Science Research Journal 8 (2020) 64-68
65
Cambodia. There were 12 questions containing in survey
including three open-ended and nine close-ended questions:
1. Sex, 2. Occupation, 3. Age, 4. Frequency of using soy
sauce, 5. Favorite taste, for knowing which taste that
Cambodian people like to eat, 6. which brand of soy sauce
that they like, production’s country and reason of their
preference, 7. Family members that they are living with, 8.
Volume of soy sauce per bottle that they use, 9. How many
bottles of soy sauce they buy per month, 10. Purchasing
location, 11. Price of soy sauce, and 12. Their support on
future soy sauce product with the good quality and safety for
comsumer.
2.2. Data analysis
Survey data were exported from online survey Google
form to an excel file, then data in excel file were transferred
to the statistical Package for Social Science (SPSS) Version
20.0 (SPSS Inc., Chicago, IL, USA). Descriptive statistic are
reported for all variables such as calculate the percentage,
validate the percentage. Pearson chi-square test was used to
evaluate associations between categorical variables.
Differences were considered statistically significant for
p < 0.05. OriginPro was used for drawing Pie diagrams
(Version 2018, OriginPro Inc.). Average daily soy sauce
comsumption was calculated by diving the total volume of
soy sauce used per month in the household with the total
members within 30 days.
2.3. Physicochemical analysis of soy sauces
The favorite soy sauce brands used by respondents were
bought from the markets to analyze their physicochemical
characteristics. The salt concentration was analyzed by using
salt-meter (Atago model ES-421), pH measurement was
analysed by using pH meter (OHAUS, Starter300), soluble
salt free solid content was analysed by following the method
as described in (GB/T, 18186-2000), amino nitrogen was
analysed as described in (GB/T, 18186-2000), and total
nitrogen was analysed as described in (AOAC, 991.20). The
soy sauce samples were analysed in triplicate and data was
represented in the form of mean ± standard deviation (SD).
Analysis of variance (ANOVA) with Tukey's test was
performed to evaluate significant difference in the
physicochemical parameters from different samples, and a
significant difference was defined as p < 0.05, and one-way
ANOVA was conducted using SPSS software.
3. RESULTS AND DISCUSSION
In the survey of 655 people, 64.4% were female and
35.6% were male. Participants were mostly in the age
between 18 to 49 years old (Table 1).
Table 1. Age of the respondents
Age
Participants
Percentage (%)
11 17
8
1.2
18 29
496
75.7
3049
130
19.8
50 64
13
2.0
65+
8
1.2
Total
655
100
According to the survey data as shown in Fig. 1, the
consumption of soy sauce in Cambodia varied. 38.8% of
respondents consumed soy sauce 2 or 3 times per week, and
27.2% of respondents consumed soy sauce 2 to 4 tims per
month. 18% of respondents consumed soy sauce 1 time per
month or least, while 16% of respondents consumed soy
sauce every day.
18%
27.2%
38.8%
16% Daily
2 or 3 times per week
2 to 4 times per month
Once a month or less
Fig. 1. Consumption of soy sauce by the respondents
The rate of soy sauce consumption in each family was
different; there were 53.4% of participants consumed soy
sauce less than one bottle per month, followed by 26.4%
who consumed soy sauce one bottle per month (Table 2).
Table 2. Consumption of soy sauce by respondents per
month
Frequency
Participants
One bottle per month
173
Two bottles per month
75
Three bottles per month
31
Least than one bottle per month
350
Other
26
Total
655
For estimating daily consumption, the total household
size, number of soy sauce bottles used per month and the
volume of soy sauce per bottle were used. As shown in
Table 3, the minimum, maximum and average household
Ly et al./Techno-Science Research Journal 8 (2020) 64-68
66
sizes in the survey consisted of 1, 14, and 4.5 people,
respectively. This number was very close with the average
number of Cambodian household size of 4.6 based on the
census data from the national institute of statistcs (NIS,
2013).
Table 3. Total number of residents currently residing in the
household
Household size
Participants
Percentage
(%)
*Valid
percentage (%)
1
42
6.4
6.5
2
76
11.6
11.7
3
83
12.7
12.8
4
132
20.2
20.3
5
146
22.3
22.5
6
75
11.5
11.6
7
49
7.5
7.6
8
20
3.1
3.1
9
7
1.1
1.1
10
15
2.3
2.3
11
2
0.3
0.3
Missing
6
0.9
Total
655
100
*Valid percentage is the percent when missing data are excluded
from the calculations.
The volume of soy sauce bottle and the design of the
bottle were also very important in attracting the consumers
as it was an innovativeness (Goldsmith et al., 2010). As
shown in Table 4, about 31.3% of respondents used 450 mL
soy sauce bottle, followed by 500 mL (20.8%) and 250 mL
(18.5%) bottles, respectively.
Table 4. The volume of soy sauce per bottle
Soy sauce
volume
Participants
Percentage
(%)
*Valid
percentage (%)
450
205
31.3
32.0
500
136
20.8
21.3
250
121
18.5
18.9
200
58
8.9
9.1
600
31
4.7
4.8
150
17
2.6
2.7
300
14
2.1
2.2
350
10
1.5
1.6
330
9
1.4
1.4
680
7
1.1
1.1
Missing
15
According to Table 2, about 53.4% of people surveyed
consumed soy sauce less than one bottle per month. As the
consumption of soy sauce in Cambodia was still low, big
bottles of soy sauce were not often produced by Cambodian
soy sauce manufacturers. Approximately 42.6% of
respondents consumed one to three bottles of soy sauce per
month and the average daily consumption is 4.6mL. Even
though soy sauce has been consumed by almost all
Cambodian people according to the survey, the consumption
of soy sauce in Cambodia was still low about 4.6 mL or
lower by comparing with the average of daily soy sauce
consumption in China 8.1 mL (CCDCP, 2002), while the
daily consumption of soy sauce in Japan was about 30 mL
per person (Kobayashi, 2013). Based on the study of fish
sauce, soy sauce, and vegetable oil fortification in Cambodia
in 2013, the average daily soy sauce consumption in
Cambodia was about 10 mL (Theary et al., 2013); therefore,
the daily soy sauce consumption in Cambodia could be
around 4.6 mL to 10 mL. Nowadays there are many soy
sauces sold in Cambodia; soy sauce has many tastes and
flavors such as saltiness, sweetness, sourness and the other
tastes that were determined by sensory test. Some soy sauces
had high amount of salt and sugars, while some other soy
sauces had low amount of sugars. For example, Shiro shoyo
or white soy sauce had salt concentration from 15% to 20%
and 16.7% reducing sugars. On the other hand, Koikuchi
shoyu or dark colored soy sauce reducing salt had salt
concentration only 8.9% and 3.53% reducing sugars
(Yokotsuka, 1986). In the survey, taste of soy sauce was
divided into 6 groups for finding the soy sauce taste that
Cambodian people like to eat such as salty, sweet, slightly
salty, slightly sweet, salty sweet, and slightly salty and
slightly sweet. As shown in Table 5, salty sweet is the taste
that the respondents like the most, followed by slightly salty.
Soy sauce brands and the soy sauce tastes were compared by
using Chi square test with the signification p < 0.05. High
consumption of salt or sodium can be very harmful for the
health, especially for the people suffering from high blood
pressure (He and MacGregor, 2010).
Table 5. Favorite taste for the respondents
Taste
Participants
Percentage (%)
p-value
Salty
31
4.7
0.007*
Sweet
31
4.7
Salty and sweet
400
61.1
Slightly salty
141
21.5
Slightly sweet
16
2.4
Slightly sweet and
slightly salty
36
5.5
Total
655
100
* Soy sauce brands and tastes were compared using Chi-square test
(Pearson) with the signification p < 0.05.
For making soy sauce, high amount of salt is needed but
it is not good for health if consume much salty soy sauce.
Seeing this issue, there is more and more interesting research
on reducing salt in soy sauce fermentation for making a
slightly salty soy sauce (Song et al., 2015). In the future as
people become more knowledgeable with healthcare, low
sodium soy sauce could be a popular product in Asian
countries.
The price of soy sauce could effect the consumption of
soy sauce in Cambodia. There were 53.10% of respondents
Ly et al./Techno-Science Research Journal 8 (2020) 64-68
67
who consumed the soy sauces that cost 2500 3900 riels
and 30.70% of respondents consumed the soy sauce that cost
4000 7900 riels, thus there were 83.8% of respondents
who consumed soy sauces that have a price between 2500
and 7900 riels. Soy sauce brands and the cost per bottle were
compared by using Chi square test with the signification
p < 0.05 (Table 6).
Table 6. Soy sauce’s cost per bottle
Price
Participants
Percentage (%)
p- value
2500 3900 Riel
348
53.10
0.001*
4000 7900 Riel
201
30.70
8000 11900 Riel
51
7.80
12000 15900 Riel
31
4.70
16000 19900 Riel
9
1.40
20000 24000 Riel
3
0.50
Higher than 240000 Riel
12
1.80
Total
655
100
*Soy sauce brands and prices were compared using Chi-square test
(Pearson) with the signification p < 0.05.
There are many brands of soy sauce sold in Cambodia
however, there are just 50 brands of soy sauce that were
mentioned by 655 respondents. There are 9 soy sauce brands
that have the percentage of consumption higher than 1%.
Among that, soy sauces C2 (Cambodia’s soy sauce), V3
(Vietnam’s soy sauce), TH5 and TH3 (Thailand’s soy
sauces) had the most consumption (Table 7).
Table 7. Favorite soy sauce brands used by respondents
Brand
Participants
Percentage
(%)
*Valid percentage
(%)
C2
212
32.4
33.4
V3
106
16.2
16.7
TH5
86
13.1
13.5
TH3
46
7.0
7.2
C5
35
5.3
5.5
J1
19
2.9
3.0
C10
15
2.3
2.4
F
10
1.5
1.6
C11
8
1.2
1.3
Missing
20
*Valid percentage (%) is the percent when missing data are
excluded from the calculations.
As described above, the favorite taste consumed by the
respondents was salty and sweet, followed by the slightly
salty. Four soy sauce brands that had the most consumption
were selected to investigate their physicochemical
characteristics to give some insight of the saltiness and their
quality. As shown in Table 8, C2 and TH3 had the salt
amount about 16.17% and 20.63%, respectively. These soy
sauces brands which were also mentioned by the
respondents in the survey that had the salty sweet taste. V3
and TH5 had salt content approximately 9.83% and 8.77%,
respectively. These soy sauce brands of the slightly salty
taste were mentioned by the participants that they like. By
comparing the results with the Cambodia soy sauce standard
CS 066:2011, TH5 was a soy sauce falling in the
specification defined by the Cambodia soy sauce standard
CS 066:2011). Not only taste and quality but also the price,
the innovativeness, and the brand loyalty that are very
important to attract the consumers (Goldsmith et al., 2010).
By looking at the price, C2 had a cheap price which costed
2000 to 2500 riels per 450 mL plastic bottle and their
handicrafts has established since 1981. V3 costed 3000 to
3500 riels per 250mL plastic bottle; TH5 costed 7000 to
8000 riels per 600mL glass bottle and TH3 costed 3500 to
4000 riels per 300 mL plastic bottle. Therefore, the price, the
taste, the design of the bottle, innovativeness, brand loyalty
and the quality are very important to attract the consumers.
Table 8. Physicochemical quality of the most consumed soy
sauces
Parameters
Soy sauce brands
C2
V3
TH5
TH3
CS
Salinity (%)
16.17± 0.06
9.83 ± 0.06
8.77 ± 0.06
20.63±0.12
≤ 20%
SSFSC (%)
2.3 ± 0.05
8.17 ± 0.14
27.72±0.18
2.97±0.61
≥ 15%
AN (%)
0.19 ± 0.03
0.25 ± 0.01
0.93 ± 0.02
0.10±0.02
≥ 0.26%
TN (%)
0.41 ± 0.05
0.6 ± 0.05
1.98±0.02
0.25±0.05
≥ 0.8%
pH
4.35 ± 0.01
4.44 ± 0.01
4.85±0.03
4.07±0.02
4.2-4.6
CS: Cambodia soy sauce standard CS 066:2011. SSFSC: Soluble
salt free solid contents. AN: Amino nitrogen. TN: Total nitrogen.
All samples were performed in triplicate, all results were
significant different (p <0.05).
Soy sauces can be found and bought at many places in
Cambodia such as home store, supermarket, local market
etc. As shown in Fig. 2, 38.46% of respondents bought soy
sauce from local markets, followed by 31.47% of
respondents who bought from the home store, 26.97% of
respondents bought from supermarket and 3.1% of
respondents bought soy sauce from other sources or
homemade.
26.97%
3.1%
38.46%
31.47%
Home store
Market
Others
Supermarket
Fig. 2. Purchasing soy sauce location by the respondents
Ly et al./Techno-Science Research Journal 8 (2020) 64-68
68
In the survey, there were 98.90% of respondents who
support the development of new soy sauce product that is
safe and has high quality. Therefore, a good quality of
Cambodian soy sauce made by fermentation should be
developed.
4. CONCLUSIONS
According to the survey, soy sauce brands (C2, V3,
TH5 and TH3) were the brands that were consumed by most
of the respondents. Among them, C2 (Cambodia’s soy
sauce) and TH5 (Thailand’s soy sauce) had salt amount
more than V3 (Vietnam’s soy sauce) and TH3 (Thailand’s
soy sauce). By comparing with Cambodia soy sauce
standard, TH5 (Thailand’s soy sauce) had higher quality
than C2, V3, and TH5. Salty sweet taste was the favorite
taste mentioned by the respondents followed by the slightly
taste. The price, the taste, the quality and the design of the
bottle are also very important to attract the consumers.
ACKNOWLEDGMENTS
We would like to acknowledge the Ministry of
Education, Youth and Sport for financial support through
Higher Education Improvement Project Research Grant.
REFERENCES
CCDCP. (2002). Food Fortification Office, Chinese Center
for Disease Control and Prevention. Retrieved on 28
August 2020 from
http://www.chinanutri.cn/FFO/related/yyzspj/201010/t2
0101025_192009.html.
Goldsmith, R.E., Flynn, L.R. & Kim, D. (2010). Status
consumption and price sensitivity. J. Mark. Theory
Pract. 18, 323338.
He, F.J. & MacGregor, G.A. (2010). Reducing Population
Salt Intake Worldwide: From Evidence to
Implementation. Prog. Cardiovasc. Dis., 52, 363–382.
https://doi.org/10.1016/j.pcad.2009.12.006
Kobayashi, M. (2005). Immunological functions of soy
sauce: Hypoallergenicity and antiallergic activity of soy
sauce. J. Biosci. Bioeng. 100, 144151
Leboffe, M.J. & Pierce, B.E. (2006). Microbiology:
Laboratory Theory and Application, Essentials -
Michael J. Leboffe, Burton E. Pierce - Google Bøker.
Morton Publishing Company.
Marcus, J.B. (2019). Flavor Enhancement Ingredients, in:
Aging, Nutrition and Taste. Elsevier, pp. 173206.
Murooka, Y. & Yamshita, M. (2008). Traditional healthful
fermented products of Japan. J. Ind. Microbiol.
Biotechnol. 35, 791798
NIS.(2013). Release of Preliminary Results of 2013 Inter-
censal Population Survey of the Kingdom of Cambodia.
Retrieved on 28 August 2020 from
http://nis.gov.kh/nis/censusinfo2.0.
Song, Y.R., Jeong, D.Y. & Baik, S.H. (2015). Effects of
indigenous yeasts on physicochemical and microbial
properties of Korean soy sauce prepared by low-salt
fermentation. Food Microbiol, 51, 171178.
Theary, C., Panagides, D., Laillou, A., Vonthanak, S.,
Kanarath, C., Chhorvann, C., Sambath, P., Sowath, S. &
Moench-Pfanner, R. (2013). Fish sauce, soy sauce, and
vegetable oil fortification in Cambodia: where do we
stand to date? Food Nutr. Bull. 34.
Yokotsuka, T. (1986). Soy sauce biochemistry. Adv. Food
Res. 30, 195–329.
GB/T 18186-2000. (2000). Nation standard of the people's
republic of china., Fermented soy sauce. Chinese
Standard Publishing House.
AOAC. (2000). Official Methods of Analysis 17th Ed.
AOAC IN-TERNATIONAL, Gaithersburg, MD,
Method 991.20.
CS 066:2011. (2012). Institue of standards of Cambodia.,
Soy sauce standard.
ResearchGate has not been able to resolve any citations for this publication.
Article
Full-text available
This paper describes a study of status consumption and price sensitivity. The pervasive desire for social prestige motivates consumers to pay higher prices for goods that confer status. We suggest that three constructs—involvement, innovativeness, and brand loyalty—mediate this relationship. We test a model using data from 409 U.S. college students. Status consumption does influence price sensitivity; and this influence is largely, but not totally, mediated by involvement, innovativeness, and brand loyalty. The results are consistent with previous studies and theories regarding these variables and attest to the importance of status consumption in consumer behavior. Understanding how such psychological constructs interact to produce their effects improves consumer theory and marketing practice.
Article
Full-text available
The prevalence of micronutrient deficiencies in Cambodia is among the highest in Southeast Asia. Fortification of staple foods and condiments is considered to be one of the most cost-effective strategies for addressing micronutrient deficiencies at the population level. The Government of Cambodia has recognized the importance of food fortification as one strategy for improving the nutrition security of its population. This paper describes efforts under way in Cambodia for the fortification of fish sauce, soy sauce, and vegetable oil. Data were compiled from a stability test of Cambodian fish sauces fortified with sodium iron ethylenediaminetetraacetate (NaFeEDTA); analysis of fortified vegetable oils in the Cambodian market; a Knowledge, Attitudes, and Practices (KAP) study of fortified products; and food fortification program monitoring documents. At different levels of fortification of fish sauce with NaFeEDTA, sedimentation and precipitation were observed. This was taken into consideration in the government-issued standards for the fortification of fish sauce. All major brands of vegetable oil found in markets at the village and provincial levels are imported, and most are nonfortified. Fish sauce, soy sauce, and vegetable oil are widely consumed throughout Cambodia and are readily available in provincial and village markets. Together with an effective regulatory monitoring system, the government can guarantee that these commodities, whether locally produced or imported, are adequately fortified. A communications campaign would be worthwhile, once fortified commodities are available, as the KAP study found that Cambodians had a positive perception of fortified sauces.
Article
Full-text available
A variety of fermentation products, such as foods containing probiotic bacteria, black rice vinegar (kurosu), soy sauce (shoyu), soybean-barley paste (miso), natto and tempeh, are sold in food stores in Japan. These fermented food products are produced by traditional methods that exploit mixed cultures of various non-toxic microorganisms. These microorganisms include lactic acid bacteria, acetic acid bacteria, sake yeast, koji molds and natto bacteria. Many traditional fermented foods have been studied and their effects on metabolism and/or immune system have been demonstrated in animal and/or human cells. This review summarizes the scientific basis for the effects of these traditional food products, which are currently produced commercially in Japan.
Article
Raised blood pressure is a major cause of cardiovascular disease, responsible for 62% of stroke and 49% of coronary heart disease. There is overwhelming evidence that dietary salt is the major cause of raised blood pressure and that a reduction in salt intake lowers blood pressure, thereby, reducing blood pressure-related diseases. Several lines of evidence including ecological, population, and prospective cohort studies, as well as outcome trials, demonstrate that a reduction in salt intake is related to a lower risk of cardiovascular disease. Increasing evidence also suggests that a high salt intake may directly increase the risk of stroke, left ventricular hypertrophy, and renal disease; is associated with obesity through soft drink consumption; is related to renal stones and osteoporosis; is linked to the severity of asthma; and is probably a major cause of stomach cancer. In most developed countries, a reduction in salt intake can be achieved by a gradual and sustained reduction in the amount of salt added to foods by the food industry. In other countries where most of the salt consumed comes from salt added during cooking or from sauces, a public health campaign is needed to encourage consumers to use less salt. Several countries have already reduced salt intake. The challenge now is to spread this out to all other countries. A modest reduction in population salt intake worldwide will result in a major improvement in public health.
Article
This chapter explains that there are major differences between the saccharification process in traditional food preparation of Western countries and that of the Orient. Shoyu is the Japanese name for soy sauce, a popular liquid condiment used in oriental cuisine. Many varieties of shoyu are produced in Japan and other oriental countries. Japanese-fermented shoyu of the koikuchi type involves five main processes—the treatment of raw materials, the making of koji, the making and aging of mash, pressing, and refining. Fifty years ago, only whole soybeans were used as the raw material for shoyu. The color and flavor of shoyu are very closely related, as both are affected by the aging of mash and the pasteurization of raw shoyu. The color of shoyu is an important attribute to Japanese dishes although it has become lighter in recent years. Using a multivariate analysis, Tanaka indicated that among the factors by which preference for a given shoyu was formed, its chemical composition as a whole contributed only 46.3%. More than 20 Japanese investigators had isolated about 130 flavor compounds from fermented shoyu by the time Goto first introduced the gas chromatography mass spectrometry (GCMS) method into this area of research in 1973, adding six new volatile compounds using this method. The precise mixture of soybeans and wheat used as the raw materials in shoyu production is the result of technological know-how developed over hundreds of years.
Article
Soy sauce is a traditional fermented seasoning of East Asian countries and is available throughout the world. In Japanese soy sauce (shoyu), soybeans and wheat are the two main raw materials, used in almost the same quantity. Proteins of the raw materials are completely degraded into peptides and amino acids by microbial proteolytic enzymes after fermentation, and no allergens of the raw materials are present in soy sauce. In contrast, polysaccharides originating from the cell wall of soybeans are resistant to enzymatic hydrolyses. These polysaccharides are present in soy sauce even after fermentation and termed shoyu polysaccharides (SPS). Soy sauce generally contains about 1% (w/v) SPS and SPS exhibit potent antiallergic activities in vitro and in vivo. Furthermore, an oral supplementation of SPS is an effective intervention for patients with allergic rhinitis in two double-blind placebo-controlled clinical studies. In conclusion, soy sauce would be a potentially promising seasoning for the treatment of allergic diseases through food because of its hypoallergenicity and antiallergic activity.
Food Fortification Office, Chinese Center for Disease Control and Prevention
CCDCP. (2002). Food Fortification Office, Chinese Center for Disease Control and Prevention. Retrieved on 28 August 2020 from http://www.chinanutri.cn/FFO/related/yyzspj/201010/t2 0101025_192009.html.
Microbiology: Laboratory Theory and Application
  • M J Leboffe
  • B E Pierce
Leboffe, M.J. & Pierce, B.E. (2006). Microbiology: Laboratory Theory and Application, Essentials -Michael J. Leboffe, Burton E. Pierce -Google Bøker. Morton Publishing Company.