Effect of Plasma Activated Water (PAW) on Fruits and Vegetables

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American Journal of Food and Nutrition, 2021, Vol. 9, No. 2, 60-68
Available online at http://pubs.sciepub.com/ajfn/9/2/1
Published by Science and Education Publishing
DOI:10.12691/ajfn-9-2-1
Effect of Plasma Activated Water (PAW)
on Fruits and Vegetables
Harsh P. Sharma1, Arpit H Patel2, Mahendra Pal3,*
1Department of Food Plant Operations, College of Food Processing Technology and Bio-energy, AAU, Anand, India
2Department of Food Processing Technology, College of Food Processing Technology and Bio-energy, AAU, Anand, India
3Narayan Consultancy on Veterinary Public Health and Microbiology, Anand, India
*Corresponding author:
Received February 14, 2021; Revised March 17, 2021; Accepted March 22, 2021
Abstract In recent time, the consumption of fruits and vegetables has raised, the variety of pathogens of fresh
fruits and vegetables has enhanced, and it's believed that pathogens are capable to resisting the stress conditions that
are major causes of fresh produce related food-borne ill health. In order to ensure microbial safety and reduce
nutrient loss, non-thermal plasma technology has received increasing attention in food preservation applications.
Non-thermal plasma has high reactivity and has potential applications in food safety, nutritional quality and
environmental safety. Plasma activated water (PAW) abundant source of reactive oxygen species (ROS) and reactive
nitrogen species, which can inactive the microorganisms. In addition to its bactericidal activity, it can also be used to
degrade pesticide residues and antibiotic residues in water and packaging materials. Non-thermal plasma is applied
to water to generate plasma-activated water, potentially applied in fruits and vegetables in recent years. PAW has
been successfully applied as washing agent or disinfect agent in fruits and vegetables. In addition, it can inactivate
food-borne pathogens on fruit and vegetable contact surfaces and on fruits and vegetables without adverse effect on
the environment and human health. Reported findings indicates that plasma activated water has the least affect the
sensory parameters and quality of fruits and vegetables. Therefore, it can be potentially applied in fruits and
vegetables industry as substitute of traditional washing agent i.e. chlorinated water, quaternary ammonium salts etc.
Furthermore, High bactericidal ability and easy to produce plasma activated water function can be used in every
food field, such as meat, dairy products, fruits and vegetables and grains. However, the chemistry of PAW is taken
into account to be extraordinarily complicated, and controlling the reaction is one in all the challenges for future
analysis. Furthermore, it also requires from regulatory agencies to generally recognize as a safe (GRAS) status.
Keywords: Fruits, Non thermal plasma (NTP), Plasma activated water (PAW), reactive oxygen species, reactive
nitrogen specie, vegetables
Cite This Article: Harsh P. Sharma, Arpit H Patel, and Mahendra Pal, “Effect of Plasma Activated Water
(PAW) on Fruits and Vegetables.” American Journal of Food and Nutrition, vol. 9, no. 2 (2021): 60-68.
doi: 10.12691/ajfn-9-2-1.
1. Introduction
Fruits and vegetables are known for their health
benefits. However, the modern food supply chain is
inherently very complex, so most foods require a certain
unit operations of processing to maintain their freshness.
Such processing may change the functional composition
of fruits and vegetables [1]. Recently, the consumption of
fresh fruits and vegetables in human diets worldwide has
rapidly increased, and consumers continue to eat more
fruits due in part to the reported health benefits. However,
minimal processing of fruits and vegetables including
washing, peeling and cutting can cause mechanical
damage to the tissue and even cause adverse consequences
such as high microbial load, browning of the cut surface,
texture destruction and off- odor development during
storage. The minimally treated surface will facilitate the
growth of certain pathogenic bacteria and spoilage
microorganisms [2]. Microbial contaminations of fresh
produce as well as foods are the main concerned in food
safety. Due to contamination, it will result in various food
born infections as well as illness. The US Centers for
Disease Control and Prevention (CDC) reports that 48
million people suffer from food-borne illnesses, of which
128,000 are hospitalized each year and approximately
3,000 die. According to the World Health Organization
(WHO) report, 600 million people worldwide experienced
food borne illness in 2010 and 420,000 deaths [3].
Pathogenic bacteria, such as Listeria monocytogenes,
Escherichia coli O157:H7, Salmonella spp., and Shigella
spp. may contaminate fresh-cut fruits and vegetables.
Therefore, it is necessary to effectively inactivate food
borne pathogens on fresh products before reaching the
final consumer, which is essential for safe food in any
food industry [4]. To ensure microbiological safety, various
convectional thermal processing have been utilized such

61 American Journal of Food and Nutrition
as pasteurization, sterilizations, canning etc. However, this
technique has been negative impact on food quality and
nutritional view. Because of this, food industry is looking
for an alternative for thermal processing. Hence, research
has been focused on novel non thermal technology for
food preservation. This non thermal technology preserved
food with minimum nutritional loss of food [5].
Non-thermal techniques can be defined as preserved
food with eliminating or minimized negative thermal
effects on nutritional and quality of food and are efficient
at ambient or sub lethal temperature. So far, the most
concerned non-thermal technologies are high pressure
processing, irradiation, pulsed electric field, ozonations,
cold plasma, etc. Among them, cold plasma technology
has been widely used in food surface decontaminations. In
addition to solids, liquids and gases, plasma is taken into
account the fourth state of matter. By addition of enough
amount of energy to material, eventually generate
electrons and ionic gases, which is called “plasma”. It
consists of positive and negative ions, free radicals,
excited and neutral atoms, electrons, ultraviolet photons,
and ground and excited molecules. According to the
thermodynamic temperature equilibrium of composition,
the plasma is divided into thermal plasma (hot) and
non-thermal (cold) plasma [6,7]. If a gas is heated to a
high enough temperature (usually around 20,000 K) for
achieve ionization of the gas, this type of plasma will be
called as “thermal plasma.” In thermal plasma, all the
chemical species compositions such as electrons and
ions are in thermodynamic temperature equilibrium.
In non-thermal plasma, it is obtained by releasing
electricity in gases and also called non equilibrium plasma
or cold plasma. The cooling of ions and uncharged
molecules is more effective than electrons to transfer
energy, and the gas remains at low temperature [6].
The temperature of the cold plasma is below 60°C,
depending on the type of plasma jet used. This cold plasma
technology is mainly used to inactivate microorganisms
and enzyme, enhance seed germinations and improve
cooking quality of rice. However, some research reports
said that due to etching and degradations of bioactive
compounds after surface treatment, some negative effects
may occur, such as loss of colour, surface topography
changes. To overcome this cold plasma problem, an
alternative method for disinfections of food is needed.
Therefore, one of the potential uses of cold plasma includes
generating plasma activated water to replace chemical
disinfectants. This plasma activated water is also called
plasma acid, plasma activated liquids, plasma treated
water containing reactive oxygen and nitrogen species,
which can be used for foods sterilization. Plasma activated
water is very easy to use and replace traditional disinfection
solutions, used for disinfections of foods [7,8].
Plasma activated water is produced using water, air and
electricity. Ambient air enters the plasma phase together
with electrical energy to produce plasma activated air. The
plasma activated air comes into contact with water.
Reactive nitrogen and oxygen (RNOS) are dissolved in
water to produce plasma activated water. The plasma
activation process takes place in the absence of any other
chemical substances and results in the product with
obvious broad-spectrum bactericidal activity. It is also
worth noting that the activity of PAW is temporary, and
PAW will return to pure water after a period of time,
further confirming the potential of this method as a green
method for inactivating pathogens [9,10].
PAW has many advantages as a disinfectant in fruits
and vegetables. Compared with traditional chemical
disinfectants, PAW is a more environmentally friendly
disinfectant [11], that is, chlorine-related products have
the risk of forming carcinogenic by-products, which has
caused more and more environmental and public health
problems. When the plasma interacts with water, various
reactive substances and some free electrons are generated.
The reactive substances present in PAW, such as active
oxygen and nitrogen substances are mainly responsible for
the antibacterial efficiency of PAW [9]. This review
focuses on the impact of plasma activated water on quality
of fruits and vegetables in terms of microbial inactivation,
quality parameters as well as sensory parameters.
2. Producing Systems of Plasma Activated
Water
The reactive species present in PAW are mainly responsible
for the inactivation of microorganisms, and their concentration
and type are affected by the gas and liquid used to generate
the plasma, the chemical environment, the excitation
voltage and the generation method. Three types of cold
plasma discharge generations are mainly used. 1) Direct
discharge 2) Indirect discharge 3) Multi-phase discharge.
Plasma jet, sliding arc discharge, dielectric barrier
discharge (DBD) and surface micro discharge (SMD) are
the most common plasma sources used to produce PAW.
This is because these types of plasma can effectively
transfer RONS from gaseous plasma to liquid phase [12].
2.1. DBD (Dielectric Barrier Discharge) and
Plasma Jet System
Basically, cold atmospheric plasma is generated by two
methods, particularly direct discharge and indirect
discharge. In indirect discharge, the active plasma species
are transported by the gas flow from the main discharge
arc. In the case of direct discharge, the product is one of
the electrodes, which is the active part of the discharge.
On the basis of these principle methods, plasma jet and
dielectric barrier discharge have been developed and
widely used in plasma food. In these two devices, a violet
plasma is generated between associate electrode i.e. anode
and cathode. Either anode or cathode is covered by a layer
of dielectric materials. Generally, quartz is used as
dielectric materials.
2.1.1. Plasma Jet Device
In these devices, the anode is connected to high voltage
and the cathode is grounded. In some plasma jet devices,
the hollow quartz tube is surrounded by a metal cathode
(i.e., copper) as a dielectric material. In addition, gases
like argon or helium are carried to maintain the formation
of cold atmospheric plasma generation. Due to the
continuous flow of the carrier gas, a cold atmospheric
plasma jet is formed. Therefore, sample is processed by
this plasma jet (Figure 1).

American Journal of Food and Nutrition 62
Figure 1. Plasma Jet and DBD Setup (Adopted from [13])
2.2.2. Dielectric Barrier Discharge
In DBD, the configuration is the same as that of plasma
jet equipment. However, this device can directly generate
plasma in the air. In some cases, carrier gases (such as
oxygen and nitrogen) have been used to produce specific
chemical components in the plasma. DBD devices tend to
produce short and wide plasmas. The main difference
between plasma jet and DBD is that the sample are part of
the discharge in case of DBD. If the sample is not close
enough to the second electrode, the cold atmospheric
plasma in the DBD will not be generated [13].
3. Physicochemical Properties of Plasma
Activated Water
When the plasma interacts with the liquid, various
complex chemical reactions occur in the interface area
between the two media, which leads to the production of
reactive substance and the physical and chemical
properties of the treated solution (including its pH,
oxidation-reduction potential (ORP)) and conductivity.
3.1. pH
pH is a measure of the concentration of hydrogen ions
in a solution. pH value is mainly caused by the formation
of nitric and nitrous acids as well as ONOOH, generated
from the NO, NO2, and NOx formed in the plasma phase.
In addition, the generation of acidic hydronium ions (H3O+)
by the reaction of the water molecules with H2O2
generated in air or liquid might also contribute to the
decrease in the pH value. Therefore, acidic pH is play a
critical role in the inactivation of microorganisms by PAW
[14].
3.2. ORP
ORP reflects the oxidation or reduction ability of a
solution, and it is related to the concentration of the
oxidant and its strength or activity. The ORP values of
PAW displayed a significant increase, mainly depending
on the plasma activation time. Of all the ROS generated in
PAW, H2O2 is believed to be mainly responsible for ORP
because it can act as an oxidizing agent (E0 = 1.77 V) or as
a reducing agent (E0= −0.7 V). Higher the ORP value,
stronger is the oxidizing capability, and higher is the
antimicrobial property [9].
3.3. Electrical Conductivity
Electrical conductivity, a measure of the ability of an
aqueous solution to conduct electricity, depends on the
types of ions, their concentrations, and the solution
temperature. The presences of extraneous ions in water
greatly affect the conductivity. The formation of ROS and
RNS during plasma activation of water will contribute to
an increase in the conductivity of PAW. The electrical
conductivity of PAW increased dramatically with the
activation time. As the pH drops, the electrical
conductivity of the solution increases which is due to the
higher mobility of H+ relative to OH− ions.
3.4. Reactive Species
During plasma discharge, various species are generated
in the gas, such as nitric oxide radical (·NO), hydroxyl
radical (·OHˉ), superoxide anion radical (·O2ˉ), atomic
oxygen (O), singlet oxygen (1O2), nitrogen ions (N2+), and
excited nitrogen molecules (N2+). When these reactive
species come into contact the liquids, numerous long-lived
reaction products are formed, e.g., hydrogen peroxide
(H2O2), nitrate (NO2ˉ), nitrite (NO3ˉ), ozone (O3),
peroxynitrite anion (ONOOˉ), and peroxynitrous acid
(ONOOHˉ). The type and concentration of the reactive
species present in the PALs are significantly affected by
the plasma device, the working gas, and the liquids used
[15].
4. Applications of Plasma Activated
Water in Food Industries
Food is contaminated and spoiled by pathogenic
organisms during the process of growth, storage,
transportation and sales, leading to major health problems
and losses of economic. Recently, PAW has shown

63 American Journal of Food and Nutrition
excellent antibacterial and anti-bio film activities and
potentially applied for removing food borne
microorganisms or disinfects food borne pathogen in
various food industries (Figure 3). In agriculture, PAW
has been used to germinate seeds and promote plant
growth. The presence of H2O2 in PAW can increase
catalase, leading to the synthesis of new proteins, thereby
helping to enhance seed germination [16]. Reference [17]
reported that rye seed germination increased by 50% when
treated with PAW for 5 minutes compared with the
control sample. In addition, the germination amount of
PAW-treated soybean seeds also increased [18].
PAW also successfully applied for the degradations of
pesticides, as a thawing agent and curing agent in meat
and meat products [12,19]. The Institute for Plasma
Research has developed a novel device and method that
can use non-thermal plasma to produce plasma activated
water. They reported that potatoes treated with PAW
shows higher germinations compared to potatoes washed
with tap water (Figure 2). In FCIPT (Facilitation Centre
for Industrial Plasma Technologies) journal organization
reported that tomatoes and potatoes washed with PAW
will make them look and maintain their freshness for
nearly 40 days at room temperature [20].
Figure 2. Potato washed with PAW and with tap water
Figure 3. Schematic representations of applications of PAW in various
food industries
As far as the fruit and vegetable industry is concerned,
the biggest challenge is its high sensitivity to post-harvest
spoilage, which leads to huge losses. Washing is one of
the important post-harvest operations to prevent fruit from
decay and obtain reasonable prices from the market.
However, this may leads in residual chlorine on the
surface of fruits and vegetables, which may cause serious
health issues. In this case, PAW is used as a substitute for
chlorinated water because of bactericide activity of
species generated during PAW generations. PAW not only
helps to reduce microorganisms, but also does not cause
changes in firmness of fruits and vegetables.
5. Effect of PAW on Microbial
Inactivation of Fruits and Vegetables
PAW has been with success applied to decontamination
of fruits like strawberries, bayberry, grapes and vegetables
(such as spinach and lettuce). Therefore, the time period
of fruits and vegetables is enhanced and therefore the
production of waste is reduced. Studies have reported that
PAW effectively inactivates aerobic bacteria, yeasts and
molds in fruits and vegetables. In addition, PAW is also
used to preserve fresh-cut fruits, such as apples, pears and
kiwis [2,21,22]. The microbial inactivation depended on
the critical parameters such as fruits and vegetables
surface texture, treatment or exposure time and power
during PAW generations. Therefore, for simultaneously
inactivate pathogenic microorganisms, optimizing these
key parameters is necessary to maintain the quality of
fruits and vegetables [3]. PAW for the inactivation of
fruits and vegetables products is outlined in Table 1.
The first study on decontamination of fruits was
reported by [4] on strawberry fruits. They found that
during processing and storage, PAW activated for 20
minutes can reduce the rate of Staphylococcus aureus
bacteria on strawberries (about 3.4 log CFU/g). Later,
similar authors reported that compared with the control,
Plasma Activated Water treatment was able to reduce the
rot of Chinese berries at the end of storage by about 50%,
indicating that PAW treatment had no effect on the
physical quality of processed foods. In case of bacteria
and fungi 1.1 log CFU/g maximum reduction was reported
at the end of storage [23]. Moreover, grapes treated with
PAW for 60 min were able to reduce Saccharomyces
cerevisiae count up to 0.53 log CFU/mL [24]. Plasma
Activated Acidified Buffer and Plasma Activated Water
were also used for decontaminations of E. aerogenes on
spiny gourds, grape tomatoes and limes [25]. The efficacy
of microbial decontamination influenced by the skin
surface, the surface of fruits and vegetables, and the
composition of texture [24]. Similarly, the smoothest
surface showed the highest reduction rate (6.32 log
CFU/surface) compared with the roughest surface (2.52
log CFU/surface) after plasma activated acidification
buffer treatment [25]. In addition to surface roughness,
other surface features, such as surface hydrophobicity and
the presence of cuticular waxes, may also influenced
inactivation efficiency [3].

American Journal of Food and Nutrition 64
Table 1. Microbial inactivation in fruits and vegetables using PAW or PAL
Fruits and Vegetables Treatment Conditions Microorganisms Log reduction References
Fresh cut iceberg lettuce Immerse the sample in the
Plasma Activated Water for 1,
3 or 5 minutes
E. coli K12, P.
fluorescens, P. marginalis,
L. innocua and P.
carotovorum
1.8 to 6.1 log [26]
Strawberries
Sample were immersed in 5, 10
and 15 min, stored 4 days S. aureus 1.7 to 3.4 log CFU/g at day 4 [4]
Chinese bayberry Samples were soaked for 0.5, 2,
or 5 min in PAW and stored at
3 C for 8 days
Total aerobic bacteria and
fungi
On days 8,
1.1 log for bacteria
1.1 log for fungi [23]
Fresh cut radicchio Treated sample with PAW for
5, 20, 40 and 60 min Listeria monocytogenes 5 log CFU/g [27]
Fresh cut celery Treated sample with PAW for
5, 20, 40 and 60 min
Listeria monocytogenes 0.57 CFU/g [27]
Grapes Soaked in PAW for 30 min S. cerevisiae
0.38 log for 30 min
0.53 log for 60 min [24]
Limes, Grape tomatoes
and spiny gourds Washed with PAW for 3 min at
50 rpm E. aerogenes B 199 A 1.03 log for spiny gourds
1.77 log for lime
1.98 for grape tomatoes [25]
Fresh cut endive lettuces Washed with or without PAW,
stored at 2 C for 7 days Total viable count 0.27 to 0.95 log CFU/g at days 7 [28]
Limes, Grape tomatoes
and spiny gourds Washed with Plasma activated
buffer for 3 min at 50 rpm E. aerogenes B 199 A 1.62 for spiny gourds
1.97 log for lime
2.00 for grape tomatoes [25]
Fresh cut kiwifruits Sprayed PAW with 1 mL on
sample and stored 8 days at 4 C S. aureus 1.8 log CFU/g at 8 th days [22]
Fresh cut iceberg lettuce Washing with PAW for 0 and
10 min P. fluorescens and L.
innocua
L. innocua : 2.4 log after 5 min
P. fluorescens: Reduce below
detectable limit [29]
Fresh cut pears Immersed in Plasma Activated
Water for 5 min and stored 12
days at 4 C
Total aerobic bacteria,
yeast and molds
Total aerobic bacteria: 0.11 to 0.65 log
Molds: 0.31 to 0.77
Yeast: 0.84 to 1.04 log
(After 12 days)
[21]
Fresh cut red leaf and
iceberg lettuce Washing with PAW for 1 or 3
min S. typhimurium
Red leaf lettuce: 2.6 log
Iceberg lettuce: 3.0 log [30]
Fresh cut spinach leaves
Rinsed the sample with Plasma
Activated Water at 120 rpm for
2 minutes and store at 4 C for 8
days
Total aerobic bacteria 1 log at 8 days [31]
Fresh cut endive lettuces
Washing with PAW with
different treatment Total aerobic bacteria
Maximum 2 log reduction at pilot
scale [32]
Fresh cut lettuces Washing with PAW with
different treatment Total Plate Count Maximum 5 log [33]
Grapes Immersed in PAW for 30 min S. cerevisiae 0.39 log [8]
Fresh cut apple Immersed apple in PAW for 5
min, stored 12 days at 4 C Total aerobic bacteria,
yeast, molds and coliform
Yeast: 1.04 log
Molds: 0.64
Total aerobic bacteria: 1.05
Coliform: maximum 5 log
[2]
Plasma activated water can be used for washing of
fresh-cut fruits and vegetables instead of chlorinated water.
For instance, fresh-cut celery and chicory treatment with
plasma activated water for 60 minutes can efficiently
decrease the amount of Listeria monocytogenes and
E. coli [27]. Reference [22] reported that PAW treatment
can reduce the populations of Staphylococcus aureus in
fresh-cut kiwifruit by approximately 1.80 log CFU/g.
Reference [21] found that PAW successfully reduced the
number of bacteria, molds, and yeasts in fresh-cut pears
Similarly, after 12 days of PAW treatment, the maximum
reduction of aerobic bacteria (number of aerobic plates),
mold, yeast and coliform in fresh-cut apples was 1.05,
0.64, 1.04 and 0.86 log CFU/g, respectively [2]. PAW
washing has also been tested on fresh-cut lettuce on a
laboratory scale and a pilot scale. Studied have shown that
total aerobic count reduced maximum 5 log and
approximate 2 log at lab scale and pilot scale, respectively.
Additionally, PAW washing treatment not adversely
effects on color, texture, surface structure and lettuce
tissue cells [33]. At the same time, by washing baby
spinach leaves with PAW, the total number of bacteria
was reduced by about 1 log CFU/mL [31].
6. Impact of PAW on Quality of Fruits
and Vegetables
Reactive oxygen and nitrogen substances present in
plasma activated water are the main factors for the change
of all the chemical properties of the processed products,

65 American Journal of Food and Nutrition
such as the nutrients and antioxidants of fruits and
vegetables. Table 2 summarizes the detailed chemical
properties of fruits and vegetables treated with PAW.
6.1. pH and Acidity
pH and acidity are key factors in most processed foods
because they reflect changes in metabolism. Various
species present in PAW, such as oxidation reduction
potential, reactive oxygen species, reactive nitrogen
species and food ingredients, including nutrients present
in processed foods, will cause pH changes [34]. Several
previous studies have reported that in fruits and vegetables,
PAW treatment does not significantly change the pH.
For instance, Reference [2] and [8] reported no difference
between fresh-cut pears and grapes respectively. Similarly,
reference [35] reported that compared with untreated
samples, the acidity of Chinese cabbage shreds
treated with plasma activated water was not statistically
different.
6.2. Vitamins
Vitamins are essential micronutrients in fruits and
vegetables. Generally, majority of vitamins are unstable
during processing, therefore it’s necessary to look into
how plasma activated water affects stability of vitamins.
According to Reference [21], there was no considerable
difference in the ascorbic acid concentration of
PAW-treated fresh-cut pears after 6 days storage period as
compared to the control. Similarly, no significant
difference observed in levels of ascorbic acid in plasma
activated water treated grapes compared to DIW and
unprocessed groups reported by [36] and [37]. However,
the ascorbic acids concentration in fresh-cut apples treated
with plasma activated water decreased after 12 days of
storage [2]. Overall, these finding show that PAW
does not significantly affect ascorbic acids levels in fruits
and vegetables. However, further research is needed to
investigate the impact of plasma activated water on other
vitamins.
6.3. Protein and enzymes
Protein and enzymes found in fruits and vegetables had
both positive and negative effects on quality of fruits and
vegetables. Enzymes are major concern in fruits and
vegetables. Peroxidase (POD) is an enzyme that causes
degradation in processed fruits and vegetables, affecting
flavor, texture, appearance, and nutrition. Reference [35]
reported that there was a statistically significant reduction
in peroxidase action in shredded salted Chinese cabbage
after plasma activated water treatment. The antioxidant
effect of enzymes is necessary to preserve the quality of
fruits and vegetables and to extend their shelf life.
Superoxide dismutase (SOD) is an enzyme that protects
fruits and vegetables from free radical damage [38].
Reference [36] reported that there was no significant
difference in superoxide dismutase activity in PAW-
soaked grapes compared with the unprocessed and DIW
groups. Additionally, superoxide dismutase activity
increased in fresh cut kiwifruits after plasma activated
water treatment [22]. Overall, PAW is a promising
technology to enhance the activity of antioxidant enzymes
and reduce certain undesirable enzymes.
6.4. Sugar Content
Carbohydrates are the most common sugar type found
in most foods. PAW not significantly affected reducing
sugar content in shredded salted Chinese cabbage
compared with control groups [35]. In addition, Reference
[36] and Xiang [8] also reported that there was no
significant change in the sugar content in grapes treated
plasma activated water as compared to commercial fresh
detergents and DIW. Although the research on the effect
of PAW on sugar content is very limited, this will be one
of the areas of interest for food scientists.
Table 2. Impact of Plasma Activated Water on quality of fruits and vegetables
Fruits and
Vegetables Conditions Protein and
enzymes Lipid/MDASugar
pH &
acidity Ascorbic
Acid
Total
phenolic References
Fresh-cut pear Plasma Activated
Water Not Available Not Available + + - [21]
Fresh-cut
kiwifruit Plasma Activated
Water
SOD (+),
POD(+),
CAT (+) Not Available Not
Available Not
Available Not Available [22]
Fresh-cut apple Plasma Activated
Water Not Available Not Available Not
Available − + [2]
Grape
Plasma Activated
Water+ mild
Heat Not Available Not Available / (+) + + + [8]
Grape Plasma Activated
Water SOD (+) Not Available / (+) Not
Available + Not Available [36]
Grape
Plasma Activated
Water
Not Available Not Available
Not
Available
Not
Available
Not Available [24]
Shredded salted
kimchi cabbage
Plasma Activated
Water+ mild
heat
POD (-) Not Available / (+) + Not
Available Not Available [35]
Note: (+): stable, Compared with the control (untreated), the treated sample has no significant difference or even improvement; (−): Significant
difference compared to control (untreated).

American Journal of Food and Nutrition 66
7. Impact of Plasma Activated Water on
Sensory Attribute of Fruits and
Vegetables
Sensory attributes include color, appearance, aroma and
taste, which are used for the evolution of sensory
performance. Several authors evaluated sensory
performance based on this parameter of PAW-treated
fruits and vegetables [4,36,39]. From the perspective of
consumers, the acceptance of fruits and vegetables mainly
depends on these sensory parameters.
7.1. Color
The presence of pigments and chemical reactions such
as enzymatic and non-enzymatic browning of fruits and
vegetables are primarily responsible for their colour. From
the consumer's point of view, color is a key parameter of
fruits and vegetables, and it is the main element of the
appearance of fruits and vegetables [40]. Color variations
in fruits and vegetables are directly observed in their
appearance and have a huge effect on customer acceptance.
The effect of PAW on the colour of fruits and
vegetables has been noted, depending on the treatment
conditions [4]. According to reference [24], there was no
major difference in the colour of grapes treated with
plasma activated water compared to control grapes. In
addition, anthocyanin content in grapes has not been
significantly affected. Reference [41] reported variations
in color index in tomato after PAW treatment. At the same
time, reference [27] noted that the color of fresh-cut
radicchio treated with PAW changed significantly. Several
studied reported no significant losses in colour of fresh cut
apple [2], fresh cut kiwifruits [22], grapes [8,24,36],
chinese berry [23], fresh cut endive lettuce [32], shredded
salted kimchi cabbage [35] and strawberries [4]. Overall,
PAW treatment did not significantly affected on color
changes in fruits and vegetables. However, plasma
activated water treatment parameters such as treatment
time, reactive substances critical factors affect the color
changes in fruits and vegetables.
7.2. Aroma and Taste
Sensory characteristics; the taste and aroma of food will
particularly affect consumers' decisions regarding food
material preferences. These characteristics guide
consumers to food sources, preferences, choices, and food
satisfaction [42]. Reference [32] reported that the test and
aroma of fresh-cut endive lettuce treated with PAW were
not significantly different from the control. This result
indicates that PAW treatment has no adverse effect on
sensory attributes.
8. Future Outlook and Concluding
Remarks
In recent years, the antibacterial activity of PAW on a
variety of fruits and vegetables has been extensively
studied. The data collected so far indicate that PAW can
be used to assure the microbiological safety and quality of
fruits and vegetables. However, the practical application
of PAW in fruits and vegetables preservation and
processing still needs further research. In contrast to
plasma treatment, PAW is considered to be cheaper and
easier to obtain. Therefore, its application has the potential
to be used on a commercial scale. However,
comprehensive safety assessment PAW must be carried
out before practical applications in different areas of the
food industry, and regulatory guidelines should be
established. Besides that, the toxicity of reactive species
and final reaction products created by chemical
decontamination, further study into PAW's applications in
food is needed. The surface of fruits and vegetables will
also affect the inactivation efficiency of PAW, which will
attract more attention in future research. Thus, optimizing
processing parameters such as surface characteristics of
fruits and vegetables, PAW production, exposure time, etc.
will require further research for effective inactivation of
microorganisms in fruits and vegetables. In the future, it is
necessary to combine other antiseptic technologies (such
as ultrasound, mild temperature, etc.) to pay attention to
the synergistic effect of microbial safety in fruits and
vegetables. Therefore, the impact on quality attributes is
minimal. However, the sustainability of this technology is
dependent on its ability to expand in the future and to
operate continuously with minimal maintenance.
In concluding remarks, PAW is an emerging technology
that can disinfect the surfaces of fruits and vegetables with
minimal impact on product quality. In addition, the
literature cited in this review indicates that that PAW have
also been applied for inactivation of microorganisms on
fresh cut agriculture produce. Therefore, its wide
application in fruits and vegetables has attracted
widespread attention. In addition, it has minimum adverse
effect on the nutritional as well as sensory attribute of
fruits and vegetables as compared to commercial
disinfectant agent i.e. chlorinated water. Therefore, PAW
potentially leading to positive consumer acceptance as
compared with product treated with commercial disinfect
agent or washing agent. However, further investigations
still needed on established of legislation or regulatory
guidance specific to PAW treatment on food can be
developed by any of the regulatory bodies around the
world. In addition, optimization studies are needed to
achieve maximum inactivation efficiency and to better
understand the toxicity of reactive species generated when
PAW is produced. Overall, plasma activated water
appears to be a promising environment friendly and cost-
effective disinfectant with the potential to improve the
safety and quality of fruits and vegetables.
Contribution of Authors
All the authors contributed equally. They read the final
version, and approved it for the publication.
Conflict of Interest
The authors declare that they do not have conflict of
interest.

67 American Journal of Food and Nutrition
Source of Financial Grant
There was no financial support for this manuscript.
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