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Genetic Characterization of a Fusarium Head Blight Resistance QTL from Triticum turgidum ssp. dicoccoides

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  • Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS), Universidad Nacional del Sur (UNS)-CONICET, Bahía Blanca, Argentina
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Qfhs.ndsu-3AS in wild emmer wheat (Triticum turgidum L. ssp. dicoccoides) is a major quantitative trait locus associated with type II resistance to Fusarium head blight (FHB). It was mapped in a population obtained from durum wheat Langdon (Triticum turgidum L. ssp. durum) and disomic Langdon-T. dicoccoides 3A substitution lines. Langdon(Dic-3A)-10 showed better performance against Fusarium graminearum (Fg) infection than the Langdon variety. To identify the genes underpinning Qfhs.ndsu-3AS, we used RNA-Seq approach in Langdon(Dic-3A)-10 and Langdon at 72 h after inoculation. The reference genomes of Svevo and Zavitan representing ssp. durum and ssp. dicoccoides, respectively, were used in parallel to align the reads and identify the genes expressed during the infection. The size of the Qfhs.ndsu-3AS region spans about 24 Mb and contains about 1000 genes. Fourteen differentially expressed genes (DEGs) were mapped in the delimited region and considered candidates for resistance. Five of them combined a higher expression level in Langdon(Dic-3A)-10 and known function in defense response: three receptor protein kinases, one bZip transcription factor, and one protease inhibitor. The alignment of the reads to the two genomes also allowed the identification of 25 genes expressed but no-DEGs localized in the Qfhs.ndsu-3AS region. Their coding sequences contained 63 non-synonymous changes and one frameshift mutation that could alter the function of the proteins and eventually contribute to resistance against Fg. This list is valuable information towards understanding the mechanism involved in resistance conferred by Qfhs.ndsu-3AS.
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Plant Molecular Biology Reporter (2021) 39:710–726
https://doi.org/10.1007/s11105-020-01277-0
ORIGINAL ARTICLE
Genetic Characterization ofaFusarium Head Blight Resistance QTL
fromTriticum turgidum ssp. dicoccoides
DanielaSoresi1,2 · PaoloBagnaresi3· Juan ManuelCrescente4,5· MarinaDíaz2· LuigiCattivelli3·
LeonardoVanzetti4,5· AliciaCarrera1,6
Received: 5 June 2020 / Accepted: 23 December 2020
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021
Abstract
Qfhs.ndsu-3AS in wild emmer wheat (Triticum turgidum L. ssp. dicoccoides) is a major quantitative trait locus associated
with type II resistance to Fusarium head blight (FHB). It was mapped in a population obtained from durum wheat Langdon
(Triticum turgidum L. ssp. durum) and disomic Langdon-T. dicoccoides 3A substitution lines. Langdon(Dic-3A)-10 showed
better performance against Fusarium graminearum (Fg) infection than the Langdon variety. To identify the genes under-
pinning Qfhs.ndsu-3AS, we used RNA-Seq approach in Langdon(Dic-3A)-10 and Langdon at 72h after inoculation. The
reference genomes of Svevo and Zavitan representing ssp. durum and ssp. dicoccoides, respectively, were used in parallel
to align the reads and identify the genes expressed during the infection. The size of the Qfhs.ndsu-3AS region spans about
24Mb and contains about 1000 genes. Fourteen differentially expressed genes (DEGs) were mapped in the delimited region
and considered candidates for resistance. Five of them combined a higher expression level in Langdon(Dic-3A)-10 and known
function in defense response: three receptor protein kinases, one bZip transcription factor, and one protease inhibitor. The
alignment of the reads to the two genomes also allowed the identification of 25 genes expressed but no-DEGs localized in
the Qfhs.ndsu-3AS region. Their coding sequences contained 63 non-synonymous changes and one frameshift mutation that
could alter the function of the proteins and eventually contribute to resistance against Fg. This list is valuable information
towards understanding the mechanism involved in resistance conferred by Qfhs.ndsu-3AS.
Keywords Durum wheat· Qfhs.ndsu-3AS—Fusarium head blight· Resistance genes· RNA-Seq analysis· Variant
comparison
Introduction
Fusarium graminearum (Fg) is the most common causal
agent of Fusarium Head Blight (FHB), a re-emerging and
destructive wheat disease in warm and humid regions
worldwide (McMullen etal. 1997). FHB has been reported to
cause extensive damage through direct losses in grain yield
and quality mainly because of the presence of mycotoxins
Key message
•Qfhs.ndsu-3AS from T. dicoccoides confers FHB resistance.
RNA-Seq revealed 14 DEGs expressed during infection and 25
no-DEGs harboring non-synonyms SNPs between resistant and
susceptible genomes, representing candidate genes for Qfhs.ndsu-
3AS
* Daniela Soresi
dsoresi@criba.edu.ar
1 CERZOS-CONICET, CCT-Bahía Blanca,
BahíaBlanca8000, Argentina
2 Departamento de Biología, Bioquímica y Farmacia,
Universidad Nacional del Sur, BahíaBlanca8000, Argentina
3 Council forAgricultural Research andEconomics—Research
Centre forGenomics andBioinformatics, via S. Protaso 302,
Fiorenzuolad’Arda(PC)29017, Italy
4 Grupo Biotecnología y Recursos Genéticos, EEA INTA
Marcos Juárez, Ruta 12 km 3, 2580MarcosJuárez, Argentina
5 Consejo Nacional de Investigaciones Científicas y Técnicas
(CONICET), BuenosAires, Argentina
6 Departamento de Agronomía, Universidad Nacional del Sur,
8000BahíaBlanca, Argentina
/ Published online: 24 March 2021
1 3
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