Residential yards in the USA have for decades been dominated by manicured lawns, invasive and non-native plants, and pesticide and fertilizer inputs—with demonstrated negative effects on biodiversity. St. Louis Audubon Society’s Bring Conservation Home program (BCH) is a large-scale urban garden initiative which has enrolled over 1,100 volunteer residential landowners to improve the urban ... [Show full abstract] landscape for wildlife. BCH supports a full-time consultant who visits and certifies each yard based on its habitat-based suitability for wildlife. With an emphasis on pollinators and birds, certification levels align with the amount of native vegetation cover, invasive plant cover and removal, and other types of habitat provisioning such as water and vertical plant diversity. We are collaborating with BCH to assess the effectiveness of this program in creating functional habitat for several taxa, and to evaluate the role of residential gardens in reducing some of the impacts of urbanization on biodiversity. To date, 395 residents have volunteered to participate in the research. With yard locations and landcover data we have quantified four different measures of urbanization within a 500 m radius of each yard: proportion of impervious surface, housing and population density, and nighttime light radiance. We calculated a composite variable of urbanization, with PC1 explaining 78% of the variability in the urbanization measures, and loaded uniformly and positively by each. We stratified yards by extent of composite urbanization and selected 21 spatial clusters of three yards with different BCH certification levels (none, silver, or gold/platinum). With these 63 landowners, we will implement a bioacoustics study aimed at estimating yard occupancy of vocalizing birds, bats, and frogs. Yard residents will place AudioMoth recording devices pre-programmed to sample different frequencies during civil twilight, dawn, dusk, and night hours. After retrieval, a validated machine-learning community-science approach will be used to identify and analyze recorded sounds.