We describe two new species of glassfrogs of the genus Centrolene living in syntopy at
La Enramada, province of Azuay, southwestern Ecuador. They were found in a small
creek in montane evergreen forests at 2,900 m elevation. The first new species is
distinguished from all other members of the genus Centrolene by having the
following combination of characters: dentigerous process of vomer absent;
... [Show full abstract] sloping
snout in lateral view; thick, white labial stripe and a faint white line between the lip
and anterior ¼ of body; humeral spine in adult males; parietal peritoneum covered by
iridophores, visceral peritonea translucent (except pericardium); ulnar and tarsal
ornamentation; dorsal skin shagreen with dispersed warts; uniform green dorsum
with light yellowish green warts; and green bones. The new species is remarkable by
being sister to a species from the opposite Andean versant, C. condor. The second
new species is distinguished from all other Centrolene by having the following
combination of characters: dentigerous process of vomer absent; round snout in
lateral view; thin, yellowish labial stripe with a row of white tubercles between the lip
and arm insertion, and a yellowish line between arm insertion and groin; uniform
green dorsum; humeral spine in adult males; parietal peritoneum covered by
iridophores, visceral peritonea translucent (except pericardium); dorsal skin
shagreen with dispersed spicules; ulnar and tarsal ornamentation; and green bones.
The second new species is sister to C. sabini and an undescribed species of Centrolene
from southeastern Ecuador. Based on nuclear and mitochondrial DNA sequences, we
present a new phylogeny for Centrolene and comment on the phylogenetic
relationships inside the genus.
