Access to this full-text is provided by MDPI.
Content available from Biomolecules
This content is subject to copyright.
biomolecules
Review
Solidago virgaurea L.: A Review of Its Ethnomedicinal
Uses, Phytochemistry, and Pharmacological Activities
Cornelia Fursenco 1, 2, †, Tatiana Calalb 1 ,†, Livia Uncu 2,3 , Mihaela Dinu 4 ,* and
Robert Ancuceanu 4
1Departament of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy,
Nicolae Testemitanu SUMPh, 66 Mălina MicăStreet, MD-2025 Chisinau, Moldova;
cornelia.fursenco@usmf.md (C.F.); tatiana.calalb@usmf.md (T.C.)
2Scientific Center of Medicines, Faculty of Pharmacy, Nicolae Testemitanu SUMPh, 66 Mălina MicăStreet,
MD-2025 Chisinau, Moldova; livia.uncu@usmf.md
3Departament of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy,
Nicolae Testemitanu SUMPh, 66 Mălina MicăStreet, MD-2025 Chisinau, Moldova
4Department of Pharmaceutical Botany and Cell Biology, Faculty of Pharmacy,
Carol Davila University of Medicine and Pharmacy, 6 Traian Vuia Street, Sector 2, 020956 Bucharest,
Romania; robert.ancuceanu@umfcd.ro
*Correspondence: mihaela.dinu@umfcd.ro
†Denotes equal contribution.
Received: 13 October 2020; Accepted: 26 November 2020; Published: 30 November 2020
Abstract:
Solidago virgaurea L. (European goldenrod, Woundwort), Asteraceae, is a familiar medicinal
plant in Europe and other parts of the world, widely used and among the most researched species
from its genus. The aerial parts of European goldenrod have long been used for urinary tract
conditions and as an anti-inflammatory agent in the traditional medicine of different peoples. Its main
chemical constituents are flavonoids (mainly derived from quercetin and kaempferol), C6-C1 and
C6-C3 compounds, terpenes (mostly from the essential oil), and a large number of saponin molecules
(mainly virgaureasaponins and solidagosaponins). Published research on its potential activities is
critically reviewed here: antioxidant, anti-inflammatory, analgesic, spasmolitic, antihypertensive,
diuretic, antibacterial, antifungal, antiparasite, cytotoxic and antitumor, antimutagenic, antiadipogenic,
antidiabetic, cardioprotective, and antisenescence. The evidence concerning its potential benefits is
mainly derived from non-clinical studies, some effects are rather modest, whereas others are more
promising, but need more confirmation in both non-clinical models and clinical trials.
Keywords:
Solidago vigaurea L.; European goldenrod; asteraceae; ethnomedicinal; phytochemistry;
distribution; pharmacological activity
1. Introduction
The genus Solidago includes about 190 species and infraspecific taxons (subspecies and varieties)
with an accepted status and about 330 species and intraspecific taxons with an ambiguous status [
1
].
They are widespread throughout the world, most of them originating from North America or confined
to this part of the world [2]. Most of Solidago species are herbaceous flowering plants, which occur in
the spontaneous flora or are cultivated as decorative plants [
3
]. Raw materials of goldenrods have a
long and wide use history in the traditional medicine of different parts of the world: S. virgaurea L.
(European goldenrod) is the most used in Europe and Asia; S. canadensis L. (Canadian goldenrod),
S. gigantea Aiton (Giant goldenrod), and S. odora Aiton—in North America; S. chilensis Meyen
−
in South
America [
4
,
5
]. According to the Flora Europaea, on the continent, there are 5 representatives of the
genus Solidago:S. virgaurea L., S. canadensis L., S. gigantea Aiton., S. altissima L., and S. graminifolia
Biomolecules 2020,10, 1619; doi:10.3390/biom10121619 www.mdpi.com/journal/biomolecules
Biomolecules 2020,10, 1619 2 of 31
L. Salisb.) [
6
]. Today, S. graminifolia is considered a synonym for Euthamia graminifolia (L.) Nutt. [
7
].
S. canadensis and S. gigantea, although of North American origin, have become widespread across
Europe and are considered “serious invaders”, whereas S. rugosa, of the same origins, has been reported
only in a few Western European countries [8].
The aerial parts of European goldenrod have been known and used for centuries as
anti-inflammatory, spasmolytic, and diuretic remedies in the traditional medicine for the treatment of
numerous diseases, especially as a urological agent in kidney and bladder inflammation, urolithiasis,
and cystitis [
3
,
4
,
8
–
12
]. According to the European Medicines Agency, S. virgaurea is one of the most
used and studied species of the Solidago genus in Europe [9].
The growing interest for the species S. virgaurea as a medicinal plant led us to carry out this
review using the most relevant and recent international research studies. The scientific community
interest in S. virgaurea is booming. Figure 1depicts the cumulative number of articles (total of 580)
published on S. virgaurea in the period 1944–2020. For this purpose, two well-known and worldwide
applied scientific databases (MEDLINE (PubMed) and HINARI), as well as Google Scholar, were used.
The number of published paper on this species has escalated in the last two decades (2000–2020).
Biomolecules 2020, 10, x 2 of 31
Meyen−in South America [4,5]. According to the Flora Europaea, on the continent, there are 5
representatives of the genus Solidago: S. virgaurea L., S. canadensis L., S. gigantea Aiton., S. altissima L.,
and S. graminifolia L. Salisb.) [6]. Today, S. graminifolia is considered a synonym for Euthamia
graminifolia (L.) Nutt. [7]. S. canadensis and S. gigantea, although of North American origin, have
become widespread across Europe and are considered “serious invaders”, whereas S. rugosa, of the
same origins, has been reported only in a few Western European countries [8].
The aerial parts of European goldenrod have been known and used for centuries as anti-
inflammatory, spasmolytic, and diuretic remedies in the traditional medicine for the treatment of
numerous diseases, especially as a urological agent in kidney and bladder inflammation, urolithiasis,
and cystitis [3,4,8–12]. According to the European Medicines Agency, S. virgaurea is one of the most
used and studied species of the Solidago genus in Europe [9].
The growing interest for the species S. virgaurea as a medicinal plant led us to carry out this
review using the most relevant and recent international research studies. The scientific community
interest in S. virgaurea is booming. Figure 1 depicts the cumulative number of articles (total of 580)
published on S. virgaurea in the period 1944–2020. For this purpose, two well-known and worldwide
applied scientific databases (MEDLINE (PubMed) and HINARI), as well as Google Scholar, were
used. The number of published paper on this species has escalated in the last two decades (2000–
2020).
Figure 1. Cumulative number of citations on S. virgaurea. Source: PubMed.
2. General Description, Taxonomy, and Distribution
S. virgaurea is a perennial herb provided with an oblique, woody rhizome, of a cylindrical shape
and devoid of knots, on which stem scars are visible (Figure 2A). The round, erect stem may achieve
a height of up to 1 m, and is ramified and pubescent at the topside. The leaves, with an alternate
arrangement, are simple, slightly pubescent on the adaxial face, and pubescent on the abaxial one.
Basal leaves have ovate or ovate-elliptic blades with an acute tip and a winged petiole, whereas upper
leaves have short stalks and linear-lanceolate or elliptic blades, with margins either serrated or entire
(Figure 2B,C). The radiate flower heads have morphologically distinguished ray (female, tongue-like)
and disc yellow florets (hermaphrodite, tubular). The flowers capitula are grouped in a simple raceme
or in panicles (Figure 2D). The receptacle is glossy and flat. The fruit is a cylindrically-shaped achene,
with 8–10 ribs and a pappus derived from the modified calyx [13].
Figure 1. Cumulative number of citations on S. virgaurea. Source: PubMed.
2. General Description, Taxonomy, and Distribution
S. virgaurea is a perennial herb provided with an oblique, woody rhizome, of a cylindrical shape
and devoid of knots, on which stem scars are visible (Figure 2A). The round, erect stem may achieve
a height of up to 1 m, and is ramified and pubescent at the topside. The leaves, with an alternate
arrangement, are simple, slightly pubescent on the adaxial face, and pubescent on the abaxial one.
Basal leaves have ovate or ovate-elliptic blades with an acute tip and a winged petiole, whereas upper
leaves have short stalks and linear-lanceolate or elliptic blades, with margins either serrated or entire
(Figure 2B,C). The radiate flower heads have morphologically distinguished ray (female, tongue-like)
and disc yellow florets (hermaphrodite, tubular). The flowers capitula are grouped in a simple raceme
or in panicles (Figure 2D). The receptacle is glossy and flat. The fruit is a cylindrically-shaped achene,
with 8–10 ribs and a pappus derived from the modified calyx [13].
A recent morpho-anatomical local study focused on the morphological and anatomical
investigation of S. virgaurea from flora of the Republic of Moldova, and its herbal product Solidaginis
Biomolecules 2020,10, 1619 3 of 31
virgaureae herba, was done by Calalb T. et al. [
13
]. The anatomical features of the highest interest for
the exact identification of S. virgaurea, established in this study, include: repartition of stomata on
both sides of the leaf, anomocytic arrangement of stomata, cone-shaped or fan-shaped multicellular
trichomes on both epidermises, as well as glandular trichomes, dorsiventral structure of the leaf with
vascular bundles collateral and open, and secretory ducts in the stem. The anomocytic stomata reported
for the leaf is consistent with the findings of Szymura M. and Wolsky K. [
14
], who also established
that anomotetracytic stomata were the most widespread type in other Solidago taxa collected from
Poland. The presence of two categories of multicellular trichomes (cone-shaped and fan-shaped) on
the goldenrod leaf was also reported by other sources [14,15]: by Buynov MV. on S. dahurica leaf [16],
by Douglas M. et al. on S. chilensis leaf [
17
], and by Fedotova VV. on S. caucasica leaf [
18
]. Care is needed
for a correct identification, because in the 19th century, a scientific paper written by a medical doctor
(based on his personal experience repeated several times) drew the attention to the risks of confusing
this medicinal plant with a non-effective (and likely dangerous for the health) Senecio nemorensis L. [
19
]
Biomolecules 2020, 10, x 3 of 31
A recent morpho-anatomical local study focused on the morphological and anatomical
investigation of S. virgaurea from flora of the Republic of Moldova, and its herbal product Solidaginis
virgaureae herba, was done by Calalb T. et al. [13]. The anatomical features of the highest interest for
the exact identification of S. virgaurea, established in this study, include: repartition of stomata on
both sides of the leaf, anomocytic arrangement of stomata, cone-shaped or fan-shaped multicellular
trichomes on both epidermises, as well as glandular trichomes, dorsiventral structure of the leaf with
vascular bundles collateral and open, and secretory ducts in the stem. The anomocytic stomata
reported for the leaf is consistent with the findings of Szymura M. and Wolsky K. [14], who also
established that anomotetracytic stomata were the most widespread type in other Solidago taxa
collected from Poland. The presence of two categories of multicellular trichomes (cone-shaped and
fan-shaped) on the goldenrod leaf was also reported by other sources [14,15]: by Buynov MV. on S.
dahurica leaf [16], by Douglas M. et al. on S. chilensis leaf [17], and by Fedotova VV. on S. caucasica leaf
[18]. Care is needed for a correct identification, because in the 19th century, a scientific paper written
by a medical doctor (based on his personal experience repeated several times) drew the attention to
the risks of confusing this medicinal plant with a non-effective (and likely dangerous for the health)
Senecio nemorensis L. [19]
Figure 2. S. virgaurea (original photo): (A) oblique rhizome, (B) basal leaves, (C) cauline leaves, (D)
radiate flower heads.
According to a number of sources [20,21], S. virgaurea is regarded as a taxonomic group or
complex, and it consists of perennial herbaceous species extensively distributed from Europe to East
Asia. As a group, it is generally divided longitudinally: in Europe the genus is represented by S.
virgaurea L., in Siberia and most of the Far East by S. dahurica (Kitag.) Kitag. ex Juz. Together with S.
spiraeifolia Fisch. ex Herder, whereas in the Far East region of Russia, known as Chukotka and in
North America, the genus is represented by S. multiradiata Ait. [22].
The European S. virgaurea L. has been described as “an exceedingly polymorphic taxon”, and a
multitude of narrowly related taxa have been included within it at different levels (varieties,
subspecies, and even species) [22,23]. In a European country (Czech Republic) flora, discussing the
variability, S. Slavik (2004) has identified 17 taxa as belonging to the S. virgaurea L. group (leaving
aside taxa from Japan), of which a number of six are qualified as subspecies, whereas a number of
eleven as “microspecies” [22,24]). The Atlas of the British and Irish flora [25] describes S. virgaurea as
highly variable, with many distinct forms for distinct habitats (ecotypes). Strong correlations have
been claimed between S. virgaurea genotypes and their geography, and a strong ability to rapidly
evolve and ecologically diversify has been recognized for the species [26].
Figure 2.
S. virgaurea (original photo): (
A
) oblique rhizome, (
B
) basal leaves, (
C
) cauline leaves,
(D) radiate flower heads.
According to a number of sources [
20
,
21
], S. virgaurea is regarded as a taxonomic group or complex,
and it consists of perennial herbaceous species extensively distributed from Europe to East Asia. As a
group, it is generally divided longitudinally: in Europe the genus is represented by S. virgaurea L.,
in Siberia and most of the Far East by S. dahurica (Kitag.) Kitag. ex Juz. Together with S. spiraeifolia
Fisch. ex Herder, whereas in the Far East region of Russia, known as Chukotka and in North America,
the genus is represented by S. multiradiata Ait. [22].
The European S. virgaurea L. has been described as “an exceedingly polymorphic taxon”, and a
multitude of narrowly related taxa have been included within it at different levels (varieties, subspecies,
and even species) [
22
,
23
]. In a European country (Czech Republic) flora, discussing the variability,
S. Slavik (2004) has identified 17 taxa as belonging to the S. virgaurea L. group (leaving aside taxa
from Japan), of which a number of six are qualified as subspecies, whereas a number of eleven as
“microspecies” [
22
,
24
]). The Atlas of the British and Irish flora [
25
] describes S. virgaurea as highly
variable, with many distinct forms for distinct habitats (ecotypes). Strong correlations have been
claimed between S. virgaurea genotypes and their geography, and a strong ability to rapidly evolve and
ecologically diversify has been recognized for the species [26].
Biomolecules 2020,10, 1619 4 of 31
3. Synonyms and Common Names
According to the Assessment Report on Solidago virgaurea, realized by the European Medicines
Agency [
9
] and the World Flora Online [
1
], the main synonyms in use are: Amphiraphis leiocarpa
Benth., Amphiraphis pubescens DC., Aster virgaurea (L.) Kuntze, Dectis decurrens Rafin. (Lour.), and Doria
virgaurea Scop. S. patagonica Phil. is currently an accepted name [
1
], although it was reported that one
Argentine specimen identified as S. patagonica was in fact an escaped cultivar of S. virgaurea [27].
The most often used common name for S. virgaurea, as well as for other Solidago species is
goldenrod. Sometimes the “European” qualifier is added to this vernacular name and in this paper,
in order to avoid confusion with other species of the genus, we will hereafter use this name (European
goldenrod). According to the American Botanical Council, other common names in use are: Solidago,
Virgaurea, Woundwort, Aaron’s rod, and Yellowweed [
28
]. The dried flowering aboveground parts
are the subject of a European herbal monograph [29].
4. Ethnomedicinal Uses
S. virgaurea has a diversity of medicinal uses in the territories where it is spread. Probably its
most widely known ethnopharmacological uses are related to kidney disorders (being often found in
teas intended to help pass kidney calculi), urinary tract infections, the overactive bladder syndrome,
and prostatic diseases [
30
–
32
], the urologic uses of the plant going back at least to the writings of Arnold
von Villanova (1240–1311) [
33
]. Traditionally, the aerial parts of the plant have been used for healing
and antiseptic properties [
9
], as well as for the treatment of diabetes, allergies, and gastro-intestinal
disorders [
8
,
32
]. Likewise, infusions or decoctions prepared from European goldenrod is used in the
traditional medicine in many parts of the world for its antibacterial and anti-inflammatory effects [
34
],
including inflammation of the oral cavity and throat, when used as a mouth rinse [
32
]. Further scientific
studies have shown the growing importance of European goldenrod as a source for herbal drugs [
35
].
In many European countries, the herbal product derived from the species has often been in combination
products [9].
4.1. Germany
Hieronymus Bock (1498–1554), one of the first modern botanists in Germany, conjectured that
Germanic tribes had been using the plant for medicinal purposes, mentioning that they regarded it as a
“miracle herb” (Wunderkraut) [
36
]. It is believed that the German father of Reformation, Martin Luther
(1438-1546), had a good opinion on goldenrod and used it often to care for his physical infirmities [
33
].
One of the first reports on its diuretic and anti-inflammatory effects are ascribed to the “father of
German botany“ (1525–1590), Jacobus Theodorus Tabernaemontanus [
37
], who stated that it “also
cleanses the kidneys and urinary tract of all coarse mucus” [
36
]. The name Heydnisch Wundkraut
(heathen woundwort), employed in the German territories during the Middle Ages for the plant,
evokes the healing properties of the herb. Another vernacular German name, Unsegenkraut (curse herb),
indicates belief at that time in its magic abilities, in an era where disease was often attributed to witchcraft
and metaphysical causes, and indirectly the name might still point towards its potential medicinal
properties [
38
]. In German folk medicine, goldenrod was used for the treatment of urinary retention,
kidney stones, and hemorrhoids [
36
]. Since the middle 19th century, its use was slowly forgotten in
Germany, to be revived only relatively recently with the renewed interest for the herbal therapy [36].
Currently, a well-established use is accepted in Germany for inflammatory diseases of the urinary
tract collection system, urolithiasis and renal gravel. A variety of extracts are used, particularly
dried extracts obtained from the aerial parts (Solidaginis virgaureae herba) using 30–60% ethanol as
an extraction solvent [
9
]. A monograph of the species was introduced in DAB in 2002, which also
acknowledged S. canadensis and S. gigantea as valid species, despite certain differences in the spectrum
of their phytochemicals [33].
Biomolecules 2020,10, 1619 5 of 31
4.2. Czech Republic
The herbal drug obtained from S. virgaurea is included in the Czech Pharmacopoeia 2009 [
39
].
A drink obtained from the aerial parts of the plant is used as an adjuvant treatment in inflammatory
conditions of the urinary system, as well as for the prevention of kidney and bladder calculi [
9
]. It is
not clear, though, whether such uses represent an old Czech tradition, as a Czech paper on the species
only cited foreign sources when referring to the traditional medicinal use of the species [39].
4.3. Poland
Traditionally, the infusion prepared of dried aerial parts of S. virgaurea has been used as a diuretic
and as an adjuvant in treatment of minor complaints of the urinary tract [
9
]. In a Polish source,
it is stated that raw material of this herb is characterized by diuretic, detoxifying, anti-inflammatory,
and bile secretion enhancing properties [
35
]. A Polish source mentions its disinfectant properties as
the most important among the traditional uses, but also its useful effects in accelerating wound healing
and in skin care [40].
4.4. Russian Federation
In Russian folk medicine, European goldenrod is used for a variety of conditions, from gallstone
disease to indigestion, and from rheumatism to gout. For external use, fresh leaves are recommended
in abscesses and boils [
41
]. Other Russian sources [
35
,
42
] state that most common uses of this
species include prevention and treatment of various diseases of the kidneys, bladder, and prostate
gland (i.e., the traditional use most widely acknowledged in Europe). In the Russian folk tradition,
the European goldenrod is also known as a hemostatic and astringent agent, as well as a good
remedy for respiratory diseases (tonsillitis, laryngitis, acute respiratory diseases), gallstone diseases,
and pulmonary tuberculosis [41].
4.5. Ukraine
The use in tuberculosis is also well established in the Ukraine folk medicine, where the name of
the plant, zolotushnik, alludes to its use in decoctions as “a good remedy against scrofula (zolotukha)”,
but the plant was also believed to have diuretic effects [43].
4.6. Bulgaria
According to a Bulgarian source, the aerial parts of S. vigaurea L. are used as a diuretic,
antihypertensive, and expectorant, as well as in the therapy of renal deficiency and gout [44].
4.7. Romania and the Republic of Moldova
S. virgaurea has a long history of use in the Romanian traditional phytotherapy. The herbal product
Solidaginis virgaureae herba has been used in herbal therapy and marketed by specialized outlets since
1990 [
45
]. Ethnopharmacological uses of this plant are mostly related to maintaining the health of the
urinary tract and the normal functioning of the digestive system. Traditionally, it is recommended
as a diuretic, saluretic, anti-inflammatory, antiseptic, healing, or a sedative agent. For external use,
the most common application consists in administering an infusion or decoction of the aerial parts
or blooming tops in the treatment of wounds or ulcers of the oral cavity [
46
,
47
]. The external use in
rickets, also acknowledged by Romanian traditional sources [
48
], does not seem to have great benefit,
considering the current knowledge of this disease and its causes.
4.8. Korea
The root and aerial parts of S. virgaurea subsp. gigantea (Nakai) Kitam have been used as an
appetite stimulant and diuretic in Korean folk medicine, whereas the immature aerial parts are used in
the same area as food [49,50].
Biomolecules 2020,10, 1619 6 of 31
4.9. China
Decoctions obtained from the whole plant were used for their antibacterial activity, and in
respiratory tract infections for its expectorant and anti-inflammatory properties [51].
4.10. Other Uses
Besides its medicinal uses, S. virgaurea has been recognized as a first-class alternative source for the
floriculture business, both on the old continent and in the new world [
32
]. It has also been proposed to
be used as a rotation crop as a means of containing noxious weeds in an organic agriculture context [
35
].
Solidago spp., including S. virgaurea, have been claimed to have potential utility for phytoremediation
purposes, based on their ability to transfer iron from soil to plants near iron processing industrial
sites [
35
]; however, studies on other oligo-elements (such as zinc) have not identified particular
hyperaccumulator properties for the plant [
52
], and whereas a number of papers have been published
on the phytoremediation potential of S. canadensis [
53
,
54
], we could find none on S. virgaurea. The pollen
of the latter is of good quality, and its availability may contribute to long-lived bees being able to
survive a hard winter [55].
5. Phytochemistry
Extracts of S. virgaurea contain C6-C1 glycosides (virgaureoside, leiocarposide) and aglycones
(vanillic acid, gallic acid) [
4
,
9
,
56
–
58
], C6-C3 polyphenolic acids (caffeic, chlorogenic, ferulic,
synapic, 3-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic, homovanilic, acids) [
3
,
9
,
10
,
45
,
53
–
57
],
a number of flavonoid molecules (mostly quercetin and kaempferol glycosides, as well as the free
aglycons and small amounts of cyanidin derivatives) [
3
,
4
,
9
,
45
,
49
,
59
–
65
], oleanane-type triterpene
saponins [
9
,
66
–
73
], essential oils containing monoterpenes (alpha-and beta-pinene, myrcene, limonene,
sabinene) [
35
,
74
–
77
] and sesquiterpenes (germacrene D
β
-caryophyllene,
α
-humulene,), clerodane-type
diterpenes [
78
], polysaccharides [
79
], and polyacetylenes [
80
] (Table 1). The European Pharmacopoeia
monograph for Solidaginis virgaureae herba regards flavonoids as quality markers and for the product
requires a content of at least 0.5% and maximum 1.5%, expressed as hyperoside [81].
It has been speculated [
3
] that a number of the active compounds of S. virgaurea extracts
(leiocarposide, polyphenolic acids, flavonoids, saponins) exert a synergistic activity in displaying the
reported anti-inflammatory effects of the product [
56
,
93
]. The antioxidant activity has been attributed
to the polyphenolic compounds [
3
,
94
–
96
], while flavonoids are thought to be responsible for the
spasmolytic effects [3,31,97].
Table 1. Chemical compounds identified in S. virgaurea.
Chemical Compounds Place/Country of Collection References
Flavonoids (Figure 3)
Quercetin,
Quercetin-3-O-glucoside (isoquercitrin)
Quercetin-3-O-galactoside (hyperoside)
Quercetin-3-O-rhamnoside (quercitrin)
Quercetin-3-O-rutinoside (rutin)
Quercetin-3-O-arabinopyranoside (avicularin)
Kaempferol-3-O-glucoside (astragalin)
Kaempferol-3-O-rhamnoside (afzelin)
Kaempferol-3-O-rutinoside (nicotiflorin)
Kaempferol-3-O-robinobioside (biorobin)
Myricetin 3-rhamnoside (myricitrin)
Isorhamnetin-3-O-rutinoside (narcissin)
Cyanidin-3-gentiobioside
mono-C-glycosylflavones (?)
di-C-glycosylflavones (?)
Poland, Italy, Hungary,
Korea, Romania, Lithuania
Fuchs L. [82],
Budzianowski J. et al. [61],
Borkowski B. and Skrzypczakowa L. [60],
Chodera A. et al. [65],
Roslon W. et al. [59],
Pietta P. et al. [62],
Apáti P. et al. [83],
Choi SZ. et al. [49],
Tamas M. [63],
Dobjanschi L. et al. [45,64],
Kraujaliene V. et al. [84]
Biomolecules 2020,10, 1619 7 of 31
Table 1. Cont.
Chemical Compounds Place/Country of Collection References
C6-C1 Compounds (Figure 4)
Benzoic acid
3-Hydroxybenzoic acid
4-Hydroxybenzoic acid
3,4-Dihydroxybenzoic (protocatechuic) acid
Salicylic acid
Gentisic acid
Vanillic acid
Gallic acid
Leiocarposide
2-methoxybenzyl-2,6-dimethoxybenzoate
Poland
Egypt
Korea
Kalemba D. [85],
Abdel Motaal A. et al. [10],
Choi SZ. et al. [49],
Thiem, B. et al. [58],
Bajkacz S. et al. [86],
Sung JH et al. [50],
C6-C2 and C6-C3 Compounds (Figure 5)
Caffeic acid,
Chlorogenic acid
5-O-caffeoylquinic (neochlorogenic) acid
3,5-di-O-caffeoylquinic acid
3,4-di-O-caffeoylquinic acid
4,5-di-O-caffeoylquinic acid
3,4,5-tri-O-caffeoylquinic acid
Methyl 3,5-di-O-caffeoylquinate
3-hydroxyphenylacetic acid
3,4-dihydroxyphenylacetic acid
5-p-Coumaroylquinic acid
Homovanilic acid
p-Coumaric acid
Ferulic acid
Sinapic acid
Rosmarinic acid
Poland
Egypt
Korea
Iran
Kalemba D. [85],
Abdel Motaal A. et al. [10],
Choi SZ. et al. [49],
Thiem B. et al. [58],
Bajkacz S. et al. [86],
Haghi G., Hatami A. [87],
Roslon W. et al. [59],
Kraujalien˙
e V et al. [84],
M. Marksa et al. [88],
D. Fraisse et al. [89],
Borkowski B. and Skrzypczakowa L. [60],
Jaiswal R. et al. [90]
Coumarins
7-hydroxy-coumarin (umbelliferone) Czech Republic Dobias P. et al. [91]
Terpene Derivatives (Figure 6)
α-Pinene,
β-Pinene,
Sabinene
Myrcene
Limonene
β-Ocimene
Germacrene-D,
β-Caryophyllene,
α-Humulene,
Clerodane diterpenes
2,8-(cis)-(cis)-Matricaria ester Matricaria
γ-lactones
Lachnophyllum lactone
ent-germacra-4(15),5,10(14)-trien-1β-ol
β-dictyopterol
Poland, Japan,
Italy,
Russian Federation,
USA
Denmark
Korea
Kalemba D. [74],
Kalemba D. and Thiem B. [75],
Fujita S. [76],
Bertoli A. et al. [77],
Tkachev AV. et al. [35],
Goswami A et al. [92],
Starks CM. et al. [78],
Lam J. [80],
Choi S. [49]
Saponins (Figure 7)
Virgaureasaponins 1–6
Solidagosaponins X-XXIX
Bellisaponin BA2
Erythrodiol-3-acetate
Germany,
France, Romania
Japan
Bader G. et al. [34,56–59],
Chevalier M. et al. [86],
Laurençon L. et al. [61],
Dobjanschi L. et al. [85],
Inose Y. et al. [60],
Sung JH et al. [50]
Carbohydrates and Other
Compounds
Polysaccharides
α-tocopherol quinone
2-phyten-1-ol
Russian Federation
Korea
Pychenkova PA. [66],
Sung JH et al. [50]
Biomolecules 2020,10, 1619 8 of 31
5.1. Flavonoids
Among the flavonoids, rutin, quercetrin, astragalin, nicotiflorin, biorobin, and narcissin have
been considered “the most representative” [
62
], and they are accompanied by their aglycons [
49
,
84
].
More recently, flavanones aglycones and glycosides have also been detected and quantified in the
different parts of the plant: eriodictyol (the largest amount in the flowers, followed by leaves and
then stems, mostly as glycosides), naringenin (similar quantitative distribution in flowers, leaves,
and stems, mostly as glycosides), and very small amounts of hesperitin (not assessed separately
in each aerial parts) [
98
]. Eriodictyol and naringenin are present in the form of both R and S
enantiomers, whereas hesperitin was detected only as the S enantiomer [
98
]. All flavonoid heterosides
seem to be 3-O-glycosides, as for the majority of Solidago species (the notable exception being
S. graminifolia (L.) Salisb, in which mono- and di-C-glycosylflavonoids have also been reported [
58
],
but which now is considered a synonym for the Euthamia graminifolia (L.) Nutt [
1
]). The presence of
flavonoid-C-heterosides in S. virgaurea has also been occasionally claimed in secondary sources [
59
],
but we could not locate a primary reference reporting them. Cyanidin-3-gentiobioside is the main
anthocyanin present in the leaves, but at least one other cyaniding-glycosyde was reported in
very small amounts [
99
]. Flavonoid glycosides tend to be better extracted in ethanol of 70% or
higher concentrations [
83
]. As mentioned above, hyperoside is considered the key flavonoid by the
European Pharmacopoeia [
81
]. However, in one paper, quercitrin was the major phytochemical from a
quantitative standpoint [
84
], whereas other sources reported rutin as the dominant flavonoid [
59
,
60
]
(a mean content 196.42 mg 100 g
−1
reported by [
59
]). A qualitative and quantitative comparative
study of flavonoids from extracts of four Solidago spp. reported in Romanian flora was carried out by
Dobjanschi L. et al. [
38
,
49
], who found a total flavonoid content for S. virgaurea of 4.06%, expressed
as rutin. They also found that for S. virgaurea, specific was the presence of rutin and hyperoside,
whereas quercitrin (unlike the findings of V. Kraujalien
˙
e et al.) was absent [
45
,
64
]. Spasmolytic effects
(as discussed below) [
31
,
97
], diuretic activity [
10
], have been attributed to the flavonoids, and other
effects have also been specifically ascribed (at least partially) to some flavonoids, e.g., antiadipogenic
effects to kaempferol-3-O-rutinoside [100].
5.2. C6-C1 Compounds
Several main compounds with a C6–C1 have apparently been reported up to date in S. virgaurea,
two glycosides and two aglycones: virgaureoside A, a bis-desmosidic glycoside derived from
benzoic acid (2-beta-D-glucopyranosyloxybenzoic acid-2
0
-beta-D-glucopyranosyloxybenzyl ester) [
101
],
leiocarposide (2
0
-hydroxybenzyl-3-methoxybenzoate 2
0
,4-diglucoside) [
56
], vanillic acid, gallic
acid [
9
,
102
], benzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic
(protocatechuic) acid, and 2,5-dihydroxibenzoic (gentisic) acid [
85
,
86
]. Such derivatives of the benzoic
acid are often occurring in the family Asteraceae [103].
A somewhat detailed history of the discovery of the two phenolic glycosides was provided by
L. Skrzypczak et al. [
103
]. Leiocarposide is currently considered the most important and was found to
be maximally biosynthesized in the flower buds (1.60%) and in the two-year leaves, post-blooming
(1.05%) [
104
]. Similar contents (0.4–1.6%) were reported by other researchers, depending on a
number of variables such as plant height at harvest, collection time, or the natural state of the herbal
samples [
58
]. Leiocarposide has attracted interest for its pharmacological potential, being explored for
its hypothesized anti-inflammatory, analgesic, antilithiatic, and diuretic effects, as discussed below.
For plants cultivated
in vitro
, the content of leiocarposide is lower than that of naturally growing
plants (0.18% vs. 0.2–1.0%) [58].
Biomolecules 2020,10, 1619 9 of 31
Biomolecules 2020, 10, x 9 of 31
Figure 3. Representative flavonoids from S. virgaurea L.
5.2. C6-C1 Compounds
Several main compounds with a C6–C1 have apparently been reported up to date in S. virgaurea,
two glycosides and two aglycones: virgaureoside A, a bis-desmosidic glycoside derived from benzoic
acid (2-beta-D-glucopyranosyloxybenzoic acid-2′-beta-D-glucopyranosyloxybenzyl ester) [101],
leiocarposide (2′-hydroxybenzyl-3-methoxybenzoate 2′,4-diglucoside)[56], vanillic acid, gallic acid
[9,102], benzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic
(protocatechuic) acid, and 2,5-dihydroxibenzoic (gentisic) acid [85,86]. Such derivatives of the
benzoic acid are often occurring in the family Asteraceae [103].
A somewhat detailed history of the discovery of the two phenolic glycosides was provided by
L. Skrzypczak et al. [103]. Leiocarposide is currently considered the most important and was found
to be maximally biosynthesized in the flower buds (1.60%) and in the two-year leaves, post-blooming
(1.05%) [104]. Similar contents (0.4–1.6%) were reported by other researchers, depending on a number
Figure 3. Representative flavonoids from S. virgaurea L.
5.3. C6-C2 and C6-C3 Compounds
A variety of C6–C3 phenolic acids have been identified in different studies. Among the most
important are the caffeoylquinic derivatives (chlorogenic acid, but also5-O-caffeoylquinic(neochlorogenic)
acid, 5-p-coumaroylquinic acid, 3,5-di-O-caffeoylquinic, 3,4-di-O-caffeoylquinic, 4,5-di-O-caffeoylquinic
acids, methyl 3,5-di-O-caffeoylquinate, and 3,4,5-tri-O-caffeoylquinic acid, the latter showing superior
anti-inflammatory effects over the di-caffeoylquinic derivatives.) [
10
,
49
,
58
,
59
,
86
,
90
]. Caffeic, p-coumaric,
ferulic, sinapic, 3-hydroxyphenylacetic, 3,4-dihydroxyphenylacetic (DOPAC), and homovanilic acids
were also reported in phytochemical studies of S. virgaurea L. [
86
]. In one study, whereas chlorogenic
acid was detected and measured (by HPLC), ellagic and rosmarinic acids could not be detected [
87
].
Chlorogenic acid content may vary considerably, as shown in one study that measured it in samples
collected from 20 sites, which found concentrations as low as 158.99 mg/100 g and as high as
Biomolecules 2020,10, 1619 10 of 31
441.50 mg/kg [
59
]. Similarly, rosmarinic acid varied in the same study between 256.38 mg/100 g and
898.70 mg/100 g [
59
], whereas (as mentioned) in a different study, it could not be detected at all [
87
].
As already discussed in the literature [
88
], a number of variables, such as the herbal part, ontogenetic
development stage, and ambient conditions have a considerable influence on the qualitative and
quantitative content in phenolic compounds of the species.
Biomolecules 2020, 10, x 10 of 31
of variables such as plant height at harvest, collection time, or the natural state of the herbal samples
[58]. Leiocarposide has attracted interest for its pharmacological potential, being explored for its
hypothesized anti-inflammatory, analgesic, antilithiatic, and diuretic effects, as discussed below. For
plants cultivated in vitro, the content of leiocarposide is lower than that of naturally growing plants
(0.18% vs. 0.2–1.0%) [58].
Figure 4. C6-C1 compounds from S. virgaurea L.
5.3. C6-C2 and C6-C3 Compounds
A variety of C6–C3 phenolic acids have been identified in different studies. Among the most
important are the caffeoylquinic derivatives (chlorogenic acid, but also 5-O-caffeoylquinic
(neochlorogenic) acid, 5-p-coumaroylquinic acid, 3,5-di-O-caffeoylquinic, 3,4-di-O-caffeoylquinic,
4,5-di-O-caffeoylquinic acids, methyl 3,5-di-O-caffeoylquinate, and 3,4,5-tri-O-caffeoylquinic acid,
the latter showing superior anti-inflammatory effects over the di-caffeoylquinic derivatives.)
[10,49,58,59,86,90]. Caffeic, p-coumaric, ferulic, sinapic, 3-hydroxyphenylacetic, 3,4-
dihydroxyphenylacetic (DOPAC), and homovanilic acids were also reported in phytochemical
studies of S. virgaurea L [86]. In one study, whereas chlorogenic acid was detected and measured (by
HPLC), ellagic and rosmarinic acids could not be detected [87]. Chlorogenic acid content may vary
considerably, as shown in one study that measured it in samples collected from 20 sites, which found
concentrations as low as 158.99 mg/100 g and as high as 441.50 mg/kg [59]. Similarly, rosmarinic acid
varied in the same study between 256.38 mg/100 g and 898.70 mg/100 g [59], whereas (as mentioned)
in a different study, it could not be detected at all [87]. As already discussed in the literature [88], a
number of variables, such as the herbal part, ontogenetic development stage, and ambient conditions
have a considerable influence on the qualitative and quantitative content in phenolic compounds of
the species.
Figure 4. C6-C1 compounds from S. virgaurea L.
Biomolecules 2020, 10, x 11 of 31
Figure 5. C6–C2 and C6–C3 compounds from S. virgaurea L.
5.4. Coumarins
Up to date, a single paper [91] reported the presence of a coumarine compound in Solidago
virgaurea: umbeliferone (7-hydroxy-coumarin). Esculetin and scopoletin were not detected in the
species [91].
5.5. Terpene Derivatives
A number of over 60 compounds have been described in the essential oil obtained from the
flowering tops of S. virgaurea L collected in Poland [74], whereas in specimens from Lithuania, 106
compounds were identified in flowers and 95 in leaves [105]. The key compounds (as found in three
samples from three different sites of Poland) were the monoterpenes α-pinene (27.4–34.1%), myrcene
(7.8–17.9%), β-pinene (5.4–7.5%), limonene (3.0–14.1%), and sabinene (0.4–11.8%), as well as the
sesquiterpenes germacrene D (8.2–17.0%), and in smaller amounts α–humulene, β-caryophyllene,
and α–muurolene [74]. In Lithuanin specimens, the composition reported recently was rather
different: in the leaves, the most important compounds detected were caryophyllene oxide, trans-
verbenol, spathulenol, humulene epoxide II, α –pinene (only 5.21%), and germacrene D; in flowers
of the same origin, the most important compounds were caryophyllene oxide, humulene epoxide II,
germacrene D, trans-verbenol, spathulenol, and bornyl acetate [105]. Detailed information on the
chemical constituents of the essential oil is presented in Table 2.
Figure 5. C6–C2 and C6–C3 compounds from S. virgaurea L.
Biomolecules 2020,10, 1619 11 of 31
5.4. Coumarins
Up to date, a single paper [
91
] reported the presence of a coumarine compound in Solidago
virgaurea: umbeliferone (7-hydroxy-coumarin). Esculetin and scopoletin were not detected in the
species [91].
5.5. Terpene Derivatives
A number of over 60 compounds have been described in the essential oil obtained from the
flowering tops of S. virgaurea L. collected in Poland [
74
], whereas in specimens from Lithuania,
106 compounds were identified in flowers and 95 in leaves [
105
]. The key compounds (as found in
three samples from three different sites of Poland) were the monoterpenes
α
-pinene (27.4–34.1%),
myrcene (7.8–17.9%),
β
-pinene (5.4–7.5%), limonene (3.0–14.1%), and sabinene (0.4–11.8%), as well as
the sesquiterpenes germacrene D (8.2–17.0%), and in smaller amounts
α
–humulene,
β
-caryophyllene,
and
α
–muurolene [
74
]. In Lithuanin specimens, the composition reported recently was rather different:
in the leaves, the most important compounds detected were caryophyllene oxide, trans-verbenol,
spathulenol, humulene epoxide II,
α
–pinene (only 5.21%), and germacrene D; in flowers of the same
origin, the most important compounds were caryophyllene oxide, humulene epoxide II, germacrene D,
trans-verbenol, spathulenol, and bornyl acetate [
105
]. Detailed information on the chemical constituents
of the essential oil is presented in Table 2.
Table 2. Detailed composition of the essential oil obtained from S. virgaurea L. flowering tops.
Compound Proportion (%) Reference(s)
α-Pinene 0.47–36.5 [35,76–78]
Camphene 0.02–0.6 [35,76–78]
Sabinene 0.06–11.8 [35,76–78]
Myrcene 0.05–17.9 [35,76–78]
β-Pinene 0.16–13.3 [35,76–78]
3-Carene 0.1–0.7 [35,76]
α-Terpinene Tr. *–0.3 [76]
Limonene 0.07–14.8 [35,76–78]
p-Cymene Tr. *–0.77 [76–78]
(E)-β-Ocimene 0.02–4.7 [35,76,78,79]
Linalol 0.3–0.8 [76]
Nonanal Tr. **–1.4 [76,77]
trans-Verbenol Tr. *–0.7 [76,77]
trans-Pinocarveol 0.09–0.2 [76,77]
Decanal 0.04–0.7 [35,76,77]
Terpinen-4-ol 0.1–1.1 [76–78]
Borneol Tr. * [76,77]
α-Terpineol 0.13–1.89 [76–78]
γ-Terpineol 0.04–0.2 [76]
p-Cymen-8-ol 0.03–0.51 [76,78]
trans-Carveol 0.05–0.3 [76,77]
Myrtenal Tr. *–0.06 [76,77]
Geraniol 0.02–0.45 [76,78]
Biomolecules 2020,10, 1619 12 of 31
Table 2. Cont.
Compound Proportion (%) Reference(s)
Verbenone Tr. *–0.6 [76,77]
α-Cubebene Tr.*–2.35 [76–78]
δ-Elemene Tr. *–9.38 [35,76–78]
Bornyl acetate 0.13–4.52 [35,76–78]
Carvone Tr. *–0.4 [76,77]
α-Copaene Tr. *–0.64 [35,76–78]
β-Bourbonene
0.2–7.28 *
[76–78]
β-Cubebene [76–78]
β-Elemene [35,76–78]
Geranyl acetate Tr. *–0.2 [35,76]
Isobutyl benzoate Tr. * [76]
(Z)-β-Farnesene Tr. *–0.6 [76–78]
β-Caryophyllene 0.1–10.5 [35,76–78]
α-Humulene 0.1–4.1 [35,76–78]
γ-Muurolene Tr. *–1.86 [35,76–78]
Germacrene-D 0.1–17.68 [35,76–78]
Isoamyl benzoate 0.08–0.4 [35,76]
α-Muurolene Tr. *–3.6 [35,76–78]
Bicyclogermacrene Tr. *–0.9 [35,76,77]
γ-Cadinene Tr. *–0.7 [35,76]
Nerolidol 0.07–0.6 [35,76–78]
Calamenene A Tr. *–0.2 [76–78]
Caryophyllene epoxide 0.4–1.6 [76,77]
Spathulenol 0.29–11.33 [76,78]
(Z)-hex-3-enyl benzoate 0.08–0.8 [76,77]
Torreyol Tr. *–0.6 [76,77]
T-Muurolol 0.2–1.16 [76,77]
Humulene epoxide 0.2–0.5 [76,77]
α-Cadinol Tr. **–3.06 [35,76–78]
(Z)-hex-3-enyl salicylate 0.09–0.3 [76,77]
Eudesma-4(15),7-dien 0.1–0.2 [76]
Mintsulphide Tr. * [76]
Cyclocolorenone Tr. *–0.3 [76,77]
Benzyl benzoate Tr. *–57.0 [35,76–78]
Geranyl benzoate Tr. *–0.1 [76]
Benzyl salicylate 0.02–1.14 [35,76–78]
β-Phenylethyl salicylate 0.1–0.6 [76,77]
α-Thujene Tr. * [77]
Linalool 0.23–2.0 [77,78]
Perillene 0.3 [77]
Campholene aldehyde 0.6 [77]
Biomolecules 2020,10, 1619 13 of 31
Table 2. Cont.
Compound Proportion (%) Reference(s)
Pinocarvone 0.5 [77]
ar-Curcumene 0.5 [77]
Spathulenol 1.6 [77]
Salvial-4(14)-en-1-one 0.1 [77]
Torilenol 0.8 [77]
Junenol 0.2 [77]
Eudesma-4(15),7-dien-1β-ol 0.1 [77]
Acetone 0.02–0.62 [78]
Ethyl acetate 0.02–2.27 [78]
Ethyl alcohol 0.03–1.62 [78]
α-Phellandren Tr. *–1.12 [78]
β-Phellandren 0.07–0.26 *** [35,78]
Terpinolene Tr. *–0.2 [35,78]
n-Hexanol 0.02–0.10 [78]
cis-3-hexen-1-ol 0.16–1.52 [78]
trans-Linalool-oxide Tr. *–0.11 [78]
trans-sabinen-hydrate Tr. *–0.26 [78]
cis-Linalool-oxide Tr. *–0.06 [78]
Benzaldehyde 0.13–0.40 [78]
γ-Elemene Tr.*–0.06 [78]
Aromadendrene 0.03–0.20 [78]
Acetophenone Tr. *–0.19 [78]
Salicyl aldehide Tr. *–0.02 [78]
trans-β-Farnesene 0.07–4.80 [35,78]
Germacrene-B 0.03–16.63 [78]
cis-α-Farnesene Tr. *–0.41 [78]
δ-Cadinene 0.2–7.87 [35,78]
Cubenene 0.02–0.25 [78]
Benzyl alcohol 0.19–1.80 [78]
2-Phenyl ethyl alcohol 0.09–1.18 [78]
o-Methoxy benzaldehyde 0.02–0.38 [78]
Caryophyllene oxide 0.1–4.30 [35,78]
Epi-cubenol 0.04–2.79 [78]
o-Methoxy benzyl alcohol 0.07–1.29 [78]
T-Cadinol 0.12–0.76 [78]
T-Muurolol 0.25–1.16 [78]
δ-Cadinol 0.15–0.73 [78]
n-Tetracosane Tr. *–0.24 [78]
n-Pentacosane Tr. *–0.50 [78]
Biomolecules 2020,10, 1619 14 of 31
Table 2. Cont.
Compound Proportion (%) Reference(s)
Phytol 0.02–1.07 [78]
n-hexacosane Tr. *–0.30 [78]
Myristic acid Tr. *–1.30 [78]
β-Ocimene-Y/(Z)- β-ocimene 0.02–3.0 [35,78,79]
γ-Terpinene Tr. ** [35]
1-undecene Tr. **–0.1 [35]
4,8-dimethyl-1,3,7-nonatriene 0.1 [35]
Camphor Tr. **–0.2 [35]
Zingiberene 0.4–1.1 [35]
Germacrene A 0.1–0.7 [35]
(E,E)- α-farnesene 1.0–2.7 [35]
β-sesquiphellandrene 0.1–0.2 [35]
(Z)-3-hexenyl benzoate 0.1–0.4 [35]
β-Eudesmol Tr. **–0.1 [35]
Neophytadiene 0.1–0.2 [35]
2-phenylethyl benzoate Tr. **–0.4 [35]
* Tr.—traces (<0.02%); ** Tr.—traces (<0.1%) (different cut-offlevels were used in different papers to define “traces”).
*** “limonene +β-phellandrene (2:1)”: 1.8–6.4% [35].
Looking at all compounds grouped by chemical structure, the largest proportion in the Polish
samples consisted of monoterpene hydrocarbons (58–73%) and sesquiterpene hydrocarbons (17–31%);
oxygenated monoterpenes and sesquiterpenes (about 3% each), benzoic acid and salicilyc acids (about
1%) represent less than 10% of the total oil [
74
]. The essential oil of specimens cultivated
in vitro
by
micropropagation was similar in its monoterpene contents, but the sesquiterpenes were apparently less
represented for S. virgaurea L. in this case [
75
]. A.V. Tkachev et al. (2006) compared the composition
of the essential oil from the aerial parts harvested from two different heights in the Russian Altai
and found that the product harvested at a lower height (290 m) are richer in essential oil (0.22%),
which contains higher amounts of
α
-pinene and myrcene as compared to the oil (0.07%) obtained
from plants harvested at a more elevated height (650 m) [35]. The key compounds in this study were
almost the same as in the specimens from Poland, the only notable exception being a higher content in
β
-caryophyllene for both specimens (maximum content in the Polish specimens was 3.3%, whereas in
the Russian specimens the minimum content was 6.3%) [35,74].
Besides the essential oil terpenes, from the aerial parts of S. virgaurea L., a number of at least 12
cis-clerodane lactones were reported in the 1980s, eight of which were new at the time of reporting [
92
].
In 2010, an additional set of nine new clerodanes were isolated from the species (most likely the aerial
part, although up to date, many clerodanes have been isolated from roots of different Solidago species),
seven of which have the relatively atypical feature of a carboxylic acid at C-19 (solidagoic acids C-I) [
78
].
A number of polyacetylene compounds have been reported in the roots of the species (matricaria
ester, two matricaria-
γ
-lactones, one lachnophyllum lactone), that show considerable seasonal
variation [
80
]. Only the matricaria ester could be detected in the aboveground shoots, whereas
the other compounds are absent from the aerial parts [80].
Biomolecules 2020,10, 1619 15 of 31
5.6. Saponins
The saponins of S. virgaurea L. have been an object of study as early as the 1930s, but the first
data on their structure were provided by K. Hiller et al. in 1975 [
106
]. In the 1980s, G. Bader et al.
were among the first to isolate and establish the chemical structures of deacylated saponins found in
the aerial parts, named by the authors virgaureasponins 1–3 [
37
,
66
,
68
,
107
]. Around the same time,
in Japan, Y. Inose et al. isolated and elucidated, from samples of Japanese origin, the structures
of oleanane-type solidagosaponins I-XX, many of them glycosylated in position 16 of the aglycone
alone or besides position 3 [
51
,
70
], and later the same group further identified solidagosaponins
XXI-XXIX [
108
]. The same Japanese researchers reported the presence of bellisaponin BA2 [
108
], which
had been isolated previously from Bellis perennis L. [
109
]. Later, another European group isolated
virgaureasaponins 4–6 from S. virgaurea ssp. alpestris [
73
]. Quantitatively, the saponin content is similar
between S. virgaurea,S. gigantean, and S. canadensis [
71
]. Unlike S. canadensis and S. gigantea, which are
derived from byogenin and have more complex sugar chains, the saponins isolated from S. virgaurea are
derived from polygalacic acid and are acylated by carboxylic acids [
37
]. As for many phytochemicals,
some of the saponins have multiple synonyms, sometimes confusing; for instance, solidagosaponin
XVIII is also known as virgaureasaponin C [73].
5.7. Polysaccharides
Both inflorescences and leaves of S. virgaurea L. contain polysaccharides. Those isolated from
inflorescences contain uronic acids (over 40%, mostly galacturonic acid), galactose (13–18%), glucose
(7–12.5%), rhamnose (4–7.5%), arabinose (2–8%), and xylose (1–2%) residues [
79
]. The amount and the
proportion among the components vary along the growing stages of the plant [
79
]. J. Saluk-Juszczak
(2010) reported quite different proportions of sugars for the polyphenolic-polysaccharide conjugates
isolated from the flower heads (rhamnose 22.4%, fucose 5.0%, arabinose 19.2%, xylose 2.6%, mannose
1.3%, glucose 13.0%, galactose 14.0%) [110].
Biomolecules 2020, 10, x 15 of 31
Figure 6. Terpenes from S. virgaurea L.
5.6. Saponins
The saponins of S. virgaurea L. have been an object of study as early as the 1930s, but the first
data on their structure were provided by K. Hiller et al. in 1975 [106]. In the 1980s, G. Bader et al.
were among the first to isolate and establish the chemical structures of deacylated saponins found in
the aerial parts, named by the authors virgaureasponins 1–3 [37,66,68,107]. Around the same time, in
Japan, Y. Inose et al. isolated and elucidated, from samples of Japanese origin, the structures of
oleanane-type solidagosaponins I-XX, many of them glycosylated in position 16 of the aglycone alone
or besides position 3 [51,70], and later the same group further identified solidagosaponins XXI-XXIX
[108]. The same Japanese researchers reported the presence of bellisaponin BA2 [108], which had been
isolated previously from Bellis perennis L. [109]. Later, another European group isolated
virgaureasaponins 4–6 from S. virgaurea ssp. alpestris [73]. Quantitatively, the saponin content is
similar between S. virgaurea, S. gigantean, and S. canadensis [71]. Unlike S. canadensis and S. gigantea,
which are derived from byogenin and have more complex sugar chains, the saponins isolated from
S. virgaurea are derived from polygalacic acid and are acylated by carboxylic acids [37]. As for many
phytochemicals, some of the saponins have multiple synonyms, sometimes confusing; for instance,
solidagosaponin XVIII is also known as virgaureasaponin C [73].
Figure 6. Terpenes from S. virgaurea L.
Biomolecules 2020,10, 1619 16 of 31
Biomolecules 2020, 10, x 16 of 31
Figure 7. Saponins from S. virgaurea L.
5.7. Polysaccharides
Both inflorescences and leaves of S. virgaurea L. contain polysaccharides. Those isolated from
inflorescences contain uronic acids (over 40%, mostly galacturonic acid), galactose (13–18%), glucose
(7–12.5%), rhamnose (4–7.5%), arabinose (2–8%), and xylose (1–2%) residues [79]. The amount and
the proportion among the components vary along the growing stages of the plant [79]. J. Saluk-
Juszczak (2010) reported quite different proportions of sugars for the polyphenolic-polysaccharide
Figure 7. Saponins from S. virgaurea L.
6. Pharmacology
6.1. Antioxidant Properties
Joining the nutritional vocabulary of the masses at least three decades ago, the concept of
“antioxidants” remains one poorly understood in the field of life sciences, and the clinical relevance
Biomolecules 2020,10, 1619 17 of 31
of antioxidants is still rather fuzzy, with many knowledge gaps [
111
]. However, partly because the
assessment of antioxidant potential is easily accessible and relatively cheap, for many herbal products,
the antioxidant properties are evaluated repeatedly, and S. virgaurea makes no exception, the first
study on this topic dating from 1995 [
96
]. In this study, ethanolic extracts of the plant were shown
in vitro
to inhibit lipoxygenase and xanthine oxidase pathways [
96
]. A methanol extract obtained from
the young shoots and leaves had stronger antioxidant effects (measured
in vitro
with a DPPH-based
assay) than an extract prepared with hot water [
95
]. Autoclaving of an 80% ethanol extract resulted
in decreased scavenging effects on DPPH and ABTS (associated with a decline in polyphenol and
flavonoid contents), but it increased the chelating effects on ferrous ions [
112
]. Although a slightly
higher antioxidant effect was observed when using pressurized fluid extraction over the ultrasonic
extraction method on S. virgaurea L. leaves (94.0% vs. 89.0% inhibition), the difference was not
statistically significant [
91
]. Both leaf and stem powders, as well as extracts obtained from those parts,
prevented lipid oxidation when applied on ground pork samples [
113
,
114
]. Among 23 herbal species
examined for their antioxidant effect in one study, S. vigaurea L. had an average antioxidant activity
(in decreasing order, it occupied the 11th position out of 23) [44].
The key component and marker of the antioxidant properties of S. virgaurea was found by
M. Marksa et al. (2020) to be 3,5-dicaffeoylquinic acid (about half of the whole scavenging activity, as for
S. canadensis and S.
×
niederederi, but unlike S. gigantea, for which the main component responsible is
chlorogenic acid) [
88
]. For several Solidago species tested, the scavenging activities were stronger for the
leaf products than for the inflorescences [
88
]. The radical neutralizing properties of quercetin derivatives
from Solidago species was shown to be considerably lower to that of the cafeoylquinic derivatives,
but it is higher than that of kaempferol derivatives, whose antioxidant potential is insignificant [
88
].
Di-caffeoylquinic acids have a stronger radical scavenging effect than mono-caffeoylquinic acids [88].
6.2. Anti-Inflammatory Effects
The anti-inflammatory activity of S. virgaurea extracts or components isolated from the species has been
repeatedly evaluated, confirmed, andascribedto different phytochemicals from its composition. In a ratmodel,
H.J.Jackeretal. (1981)showedthatatriterpenesaponinfractionadministered i.v. atalowdose(1.25–2.5mg/kg)
caused a significant decrease of edema, as measured by pletysmograph [
9
,
115
]. Although J. Metzner et al.
(1984) reported some anti-inflammatory effect for leiocarposide (200 mg/kg) in a carrageenan-induced
edema model in rats, the effect was obviously inferior to that of phenylbutazone (e.g., 3% vs. 53%
two hours post-administration and 27% vs. 54% reduction five hours post-administration) [
9
,
56
].
Rutin and quercetin, as well as 3,5-dicaffeoylquinic acid were shown by M. Melzig et al. (2000) to
inhibit leukocyte elastase, an effect considered synergistic with the radical scavenging of the same
molecules in exerting their anti-inflammatory activity [
33
,
93
]. Instead, saponins and leiocarposide
did not demonstrate any such elastase inhibition; the same authors claimed that ester saponins
stimulate the release of ACTH by their interaction with cell membranes of the pituitary cells, and thus,
consecutively, glucocorticoids with anti-inflammatory effects [
33
,
93
]. 3,4,5-O- tricaffeoylquinic acid was
identified among phenolic compounds from S. virgaurea as having the highest anti-inflammatory effect
(88% of that of indomethacin) in rats (carrageenan-based rat paw edema) and to inhibit TNF-
α
and
IL-1
β
[
10
]. On the other hand, other studies reported stimulation of TNF-
α
secretion by macrophages,
an effect induced by different phytochemicals of the species (2-methoxybenzyl-2-hydroxybenzoate,
benzyl-2-hydroxy-6-methoxybenzoate) [
116
], and it remains to be investigated in what contexts and
under the influence of what variables one or the other effect will predominate.
Aqueous and ethanolic extracts of S. virguarea have demonstrated an ability to reduce paw edema
and arthritic paw volume in rat models of inflammation [
117
]. Some inhibition of dihydrofolate
reductase has been described for a hydroalcoholic extract of the species, and this has been suggested as
contributing to the anti-inflammatory effects of the S. virgaurea extracts, being known that inhibitors of
the enzyme, such as methotrexate, do have anti-inflammatory activity [118–120].
Biomolecules 2020,10, 1619 18 of 31
A standardized combination of alcoholic extracts of S. vigaurea,Populus tremula L., and Fraxinus
excelsior L. has been developed as an anti-rheumatic drug and has been relatively extensively
investigated [117,121,122].
6.3. Analgesic Activity
An
in vitro
study evaluated the analgesic potential of a methanol seed extract by assessing its
affinity for three receptors involved in acute pain signaling (bradykinin, neurokinin 1, and calcitonin
gene related peptide). The extract exhibited substantial binding to the bradykinin receptor, but this
effect was canceled by PVP treatment, allowing the authors to speculate that it should probably
be ascribed to non-specific binding of tannins or other polyphenols [
123
]. In the hot plate test on
mice, leiocarposide demonstrated very similar analgesic effects to aminophenazone for the first hour,
but those effects almost disappeared post-administration after the second hour [56].
6.4. Spasmolytic and Antihypertensive Activity
Ex vivo data obtained on isolated smooth muscles from guinea pig gut showed a modest
spasmolytic effect for a S. virgaurea ethanol extract (less than 15% of the papaverine effect) [
9
,
124
].
As mentioned below, extracts of S. virgaurea have demonstrated anti-muscarinic effects on isolated
bladder, inhibiting the M2 and M3 receptors [
31
]. It has been stated that all species of the genus have
“hypotensive activity”, including S. virgaurea, which demonstrated such an effect in dogs, for a leaf
extract, at a dose of 150/kg [
119
,
125
,
126
]. Aqueous extracts from flowers and from leaves, administered
by i.v. route in rats, at doses of 180 and 360 mg/kg found no reduction of blood pressure after the
first 2–5 min, for both extracts [
127
], despite isolated interpretations to the contrary [
119
]. This has
been speculatively related to a potential contribution of flavonoids, based on the reported vasodilatory
effects mediated by the inhibition of protein kinase C resulting in the relaxation of the arterial smooth
muscle [
97
]. In that study, flavonols had a stronger effect than flavones, which in their turn had a more
potent effect than flavanols [
97
], and as shown above, S. virgaurea key flavonoids are derivatives of
flavonols (quercetin and kaempferol).
6.5. Diuretic Effects and Benefits in Other Urinary Tract Conditions
As shown above, many folk traditions attribute a diuretic activity to extracts prepared
from S. virgaurea L. In academic sources, it is mentioned at least starting with the 17th century,
when Shcroeder’s “Thesaurus pharmacologicus” points out to it; throughout the 20th century, different
sources also cite this pharmacological effect in relationship to S. virgaurea products [9].
The diuretic effects have been attributed to the flavonoid fraction (particularly quercetin and
its derivatives), which was shown to inhibit the neutral endopeptidase, resulting in an enhanced
urinary flow [
88
,
93
]. The inhibition of neutral endopeptidase leads to an increase in the plasma
concentration of natriuretic peptides, which have strong natriuretic properties [
128
]. A. Chodera et al.
(1991) found that this flavonoid fraction (25 mg/kg b.w.) increases the urine output in rats by about
88%, and causes a decrease in the excretion of sodium and potassium, accompanied by an increase in
calcium excretion [
9
,
65
]. These results are in contradiction with those reported later by U. Kaspers et al.
(1998), who found no increase in urine volume or electrolytes for the flavonoid fraction [
9
,
129
].
Instead, they reported that the hydroxycinnamic acid fraction (100 mg/kg) and the saponin fraction
(25–100 mg/kg) had an effect comparable with those of furosemide [
9
,
129
], including an increase in
sodium and potassium excretion, unlike the data reported by A. Chodera et al. (1991), which claimed a
decrease in the excretion of these ions [9,65].
Besides the flavonoidic fraction, leiocarposide (25 mg/kg, i.p.) was also shown by A. Chodera et al.
(1985) to have diuretic activity, equivalent to about 75% of the furosemide effect [
9
,
130
]. The same
authors have demonstrated that the i.p. route results in higher efficacy (about 30%) than the oral route,
that it has a slow onset (around 5 h), and it lasts for up to 24 h [
9
,
119
,
131
]. The aglycone part of the
glycoside (leiocarpic acid) is devoid of diuretic activity (at the same dose, 25 mg/kg i.p.) [9,132].
Biomolecules 2020,10, 1619 19 of 31
Besides the assumed diuretic effects, S. virgaurea has been declared “the plant that is most
frequently extracted to yield preparations for the treatment of bladder dysfunction including the
overactive bladder syndrome” [
31
]. In this sense, clinical data in patients with dysuria have claimed
that S. virgaurea reduces the frequency of urination(as well as the pain associated with it). In a relatively
large (n=512 patients), but open-label and uncontrolled study of patients with chronic recurrent
overactive bladder, 96% of the subjects receiving 424.8 mg of S. vigraurea extract, t.i.d., reported an
improvement in the clinical global impression and a significant drop in painful micturition and in
the need to urinate [
133
]. In a smaller study (n=74), also open-label, carried out on female patients
with dysuria, a reduction of the same symptoms was observed in 69% of the patients [
133
]. These
results are somewhat puzzling because diuretics tend to rather increase the frequency of urination,
but the diuretic data come from non-clinical studies with an isolated compound (leiocarposide) or
the flavonoid fraction, and not with an extract as such. On the other hand, the open-label character
and the lack of a control group indicate a low quality of these data and stronger evidence is needed
to conclude on the effect S. virgaurea have on the urinary tract.
In vitro
data have demonstrated that
extracts of the plant have anti-muscarinic effects on the M2 and M3 receptors, which results in the
inhibition of the bladder contraction [31].
Leiocarposide (25 mg/kg p.o.) administered for six weeks significantly inhibited the growth of
human-derived urinary calculi transferred into the rat bladder [57].
6.6. Antibacterial Activity
An investigation of the antibacterial potential of two extracts, one alcoholic and one lipophilic
(with hexane), found that the alcoholic had the lowest MIC for Staphyllococcus aureus, whereas the
lipophilic one for S. aureus and P. aeruginosa [
134
]. The MIC values varied between 2.95 and 11.8 mg/mL
for the ethanolic; for the hexane extract, MIC was 3.5 mg/mL for Staphyllococcus aureus,Staphyllococcus
faecalis, and Pseudomonas aeruginosa, and higher than 3.5 mg/mL for the other microorganisms
tested [
134
]. Although values lower than 16 mg/mL have been considered sometimes as showing
a strong antibacterial effect [
135
], other authors have used more stringent criteria: MIC values <
100
µ
g/mL have been proposed to be highly active, those between 100 and 500
µ
g/mL active, those
between 500 and 1000
µ
g/mL moderately active, those between 1000 and 200
µ
g/mL of low activity,
and those with MIC >2000
µ
g/mL inactive [
136
,
137
]. It has also been suggested that in order to
“be considered a promising activity, a crude extract must demonstrate a MIC under 100
µ
g/mL” (and a
pure compound less than 16
µ
g/mL) [
138
]. Other studies have also assessed various extracts for
their antimicrobial effects, but MIC or MBC (minimal bactericidal concentration) was also for most
species higher than 2000
µ
g/mL [
139
–
141
]. In the light of these criteria from the literature, the activities
observed in this study are not “promising” and because they are higher than 2000
µ
g/mL, should
rather be considered inactive.
In a study comparing (among others) the ethanol and aqueous extracts obtained from leaves
and stems of S. virgaurea (agar disc diffusion method), inhibition zones were detected only for the
ethanol extract and only on Bacillus subtilis,Micrococcus flavus (Gram-positive), and the Gram-negative
Morganella morganii [
142
]. Another study also reported no inhibitory effect on several microbial species
of an aqueous extract [
72
]. It has been suggested that inhibition zones smaller than 9 mm should be
considered inactive, those ranging between 9 and 12 mm, moderately active, those ranging between
13 and 18 mm
, active, and those larger than 18 mm should be classified as very active [
136
,
143
]. Since,
in this study, all inhibition zones were less than 9 mm in size [
142
], the ethanol extract should also be
regarded as virtually inactive. A. Brantner and J. Grein (1994), though, reported inhibition zones of
around 11 mm on Staphylococcus aureus and Escherichia coli for an aqueous extract prepared from the
aerial parts of the plant [
140
]. A methanol extract produced inhibition zones of 15 mm for Staphylococcus
aureus and Bacillus cereus, and of 20 mm for Enterobacter fecalis [
95
] (i.e., the extract could be considered
active and very active, respectively, on these microbial species).
Biomolecules 2020,10, 1619 20 of 31
A root extract was fractioned in a bioassay-guided manner and the active compounds responsible
for the antibacterial effects (on Bacillus subtilis and Xanthomonas euvesicatoria, a plant pathogen) were
identified as two isomers of the matricaria ester. For the extract, the MIC determined was 172
µ
g/mL for
B. subtilis, and 86
µ
g/mL for X. euvesicatoria [
32
]. Nine clerodante-type diterpenes have been evaluated
for their activity on S. aureus, and all had a modest activity, considerably inferior to that of vancomycin
(the lowest MIC, observed for the second clerodane compound, was 30
µ
g/mL, 15 times higher than
that of vancomycin and over the 16
µ
g/mL for a pure compound to be considered “active”) [
78
].
A. V. Tkachev et al.
(2006) claimed complete inhibition of S. aureus for the essential oil [
35
], but these
positive results were observed only with the undiluted essential oil and the
1
2
dilution, which is of very
little clinical significance for systemic use.
Inhibition of bacterial cell division, impairment of plasmalema or cell membranes inside the cell
resulting in cell lysis [
134
,
144
] and inhibition of dihydrofolate reductase [
9
,
118
] have been suggested
as potential mechanisms for the supposed antibacterial effects of S. virgaurea extracts. The effect has
been attributed to several phytochemical groups: phenolic acids and flavonoids [
134
], terpenes and
essential oils [
35
,
134
], matricaria ester isomers [
32
], and clerodante-type diterpenes [
78
]. Considering
the modest antibacterial activity reported by most studies, the discussion of these mechanisms (which
are supported in a general way by the general literature on these phytochemicals [
145
–
148
]) is, in our
view, rather moot.
6.7. Antifungal Activity
G. Bader et al. investigated the antifungal potential of S. virgaurea extracts and reported that
deacylated saponins tend to have a stronger effect on different Candida species and Cryptococcus
neoformans than the corresponding mixture of ester saponins, and among the deacylated saponins,
the bidesmosides had stronger activity than monodesmosides [
9
,
66
,
67
,
119
,
149
]. S. Pepeljnjak et al.
(1998) claimed antimycotic activity on several dermatophyte species (Trichophyton mentagrophytes,
Microsporum gypseum and Microsporum canis) for a hydroalcoholic extract (75%) [
150
], but the MIC
values (between 9.7% and 50%) seem too high to support the claim for clinical purposes, the effect
being modest at best. M. Chevalier et al. (2012) reported no inhibitory effect of an aqueous extract from
S. virgaurea on four strains of Candida albicans on agar mediums or in liquid medium, but they found that
it was able to inhibit the transition between yeast and hyphal growth, a significant inhibition of biofilm
formation, as well as a decrease of pre-formed biofilms of C. albicans, an effect speculatively attributed
to the saponin fraction of the extract [
72
]. This attribution seems reasonable considering the inherent
surfactant properties of saponins, as well as their hemolytic and iron chelator qualities, iron being
necessary for the growth and development of Candida [
72
,
151
]. To confirm this, the same research
group carried out a bioassay-guided fractionation that led to the isolation of six triterpene saponins
(oleanane-type), of which four caused significant inhibition of C. albicans yeast-hyphal switch [
73
].
What is the clinical relevance of this effect only time will tell.
6.8. Antiparasite Activity
In a murine experiment, at a dose of 500 mg/kg bw, an extract of the aboveground parts of
S. virgaurea showed the largest effect on Acanthamoeba, prolonging the survival of the animals to an
average time of 12 days, whereas the controls had a mean survival time of only 4 days. Another three
species tested in the same experiment were associated with shorter survival times [
152
]. An extract
obtained from the stems did not have any remarkable activity on the parasitic nematode Haemonchus
contortus [153].
6.9. Cytotoxic and Antitumor Activity
In murine models of sarcoma (allogenic sarcoma-180 and syngenic DBA/2-MC.SC-1 fibrosarcoma),
significant antitumor activity was claimed for virgaureasaponin E administered in low doses
(1 mg/kg/day) [
9
,
68
,
69
,
154
]. This effect of viragurea saponin E may at least partly be related
Biomolecules 2020,10, 1619 21 of 31
to its ability to induce TNF
α
release from macrophages, as well as to an induction of cytotoxic
macrophages [
154
]. Data for saponins from several species, including S. virgaurea L., evaluated on
YAC-1 (T cell lymphoma) and P-815 cells (mouse mastocytoma), indicated that the glycosylation
pattern (at 3 or 28 carbon atom position) has an influence on the cytotoxicity and that similarly to the
antifungal effect, bisdesmosides derived from the polygalacic acid tend to be more active than their
related prosapogenins [
155
]. Not only virgaureasaponin E is able to activate macrophages and TNF-
α
secretion, but also two C6-C1 compounds of the herbal product (2-methoxybenzyl-2-hydroxybenzoate,
benzyl-2-hydroxy-6-methoxybenzoate) [116].
A methanol extract of S. virgaurea ssp. gigantean was reported to be active against the melanoma cell
line SK-MEL-2 [
50
]. Fractionation guided by cytotoxicity led to the isolation of three active compounds
from the hexane-soluble fraction, evaluated on five human tumor cell lines: erythrodiol-3-acetate,
α
-tocopherol quinone, and 2-phyten-l-ol [
50
]. However, the ED
50
varied between 5.9 and 54.8
µ
M,
which qualified the activity as rather modest (the most active one, with ED
50
less than 10
µ
M on four
out of the five cancer cell lines was the
α
-tocopherol quinone) [
50
]. It should also be considered, though,
that if one important mechanism of antitumor activity is the stimulation of cytotoxic macrophages and
TNF
α
release, as discussed above [
154
], a mere cytotoxicity test may not be appropriate to assess the
antitumor potential of these extracts.
Experiments on PC3 cells have shown that, whereas aqueous and ethanol extracts had strong
cytotoxic activity, chloroform extracts were devoid of such activity [
34
]. Extracts prepared from stems
were markedly less active than those prepared from leaves or flowers [
34
]. The same authors also
found that pre-treatment with protease leads to an almost complete loss of effect, which indicates that
a protein is responsible for this activity [
34
]. A rodent model of prostate cancer (AT6.1) showed that
i.p. administration in SCID mice of an active fraction corresponding to a molecular weight of about
40,000 Da (at a low dose of 5 mg/kg, once every three days) resulted in a significant reduction in tumor
volume as compared with the non-treated animals [
34
]. Since SCID mice have immune deficiencies
consisting of the lack of B and T lymphocytes, the authors speculated that the effect of this fraction
is due to a direct cytotoxic effect rather than being mediated by the immune system [
34
]. This was
also supported by the caspase-3 increase in cells treated with the fraction and the flow cytometric
analysis, which suggested that the fraction causes cell cycle arrest (in the G0/G1 phase), triggering then
apoptosis [
34
]. Since SCID mice, though, have macrophages, an immune contribution should not be
necessarily excluded, in the absence of direct evidence to the contrary.
Ent-germacra-4(15),5,10(14)-trien-1
β
-ol,
β
–dictyopterol, and 3,5-di-O-caffeoyl quinic acid exhibited
“moderate cytotoxicity” against five cancer cell lines, with ED
50
values varying between 1.52–18.57
µ
M
(the most active–ED
50
between 1.52 and 18.57
µ
M–being
β
–dictyopterol) [
49
]. Methyl 3,5-dicaffeoyl
quinate (MDQ) has demonstrated antioxidant activities, as well as antiproliferative effects
in vitro
,
in HT-29 cell, by inducing cell cycle arrest and apoptosis through inhibition of the PI3K/Akt and ERK
signaling pathways [156].
6.10. Antimutagenic Activity
Hexane extracts of three Solidago species demonstrated antimutagenic activity in a S. typhimurium
test, the most active apparently being S. virgaurea L; instead, the ethanolic extracts were devoid of any
such antimutagenic effects [134].
6.11. Antiadipogenic and Antidiabetic Activities
Following an impressive screening of about 300 plant extracts, kaempferol-3-O-rutinoside was
isolated from a butanol fraction of a water extract of S. virgaurea, showing strong anti-adipogenic
effects
in vitro
and supressing PPAR-
γ
and C/EBP
α
expression [
100
]. Of a number of several phenolic
derivatives of the species, evaluated
in vitro
for their anti-adipogenic potential, 5-di-O-caffeoylquinic
acid was found to have the most potent inhibitory effect [157]. A 10% ethanolic extract of S. virgaurea
also showed good anti-adipogenic effects
in vitro
and
in vivo
in a murine model, causing a decrease in
Biomolecules 2020,10, 1619 22 of 31
body weight, liver weight, and the magnitude of the adipose tissues [
158
]. Such experimental data are
worth further investigation of the species for its potential use for weight loss purposes.
In an alloxan-induced diabetic rat model, a hydroalcoholic extract of S. virgaurea caused a decline
in glycemia, TNF-
α
, serum amylase activity, and pancreatic malondialdehyde, as well as a rise in
serum insulin, hepatic glycogen, pancreatic SOD, and catalase activities [159].
6.12. Cardioprotective Effects
In a rat model, a protective effect against cardiotoxicity (induced with isoproterenol) was claimed
for a methanolic extract of S. virgaurea L. [160].
6.13. Antisenescence Effects
An alcoholic extract of Solidago virgaurea subsp. alpestris was claimed to exert anti-senescence
effects
in vitro
on fibroblasts [
161
], opening a door towards the possibility to use it as for anti-aging
purposes in topical or systemic preparations.
An overview of the pharmacological effects of extracts prepared from S. virgaurea L. is shown in
Table 3.
Table 3.
A summary of the pharmacological properties of extracts and fractions obtained from
S. virgaurea L.
Pharmacological
Properties Evidence
Chemical Compounds/
Fraction to Which the
Property is Ascribed
Critical Assessment of Study
Results
Antioxidant effects In vitro Caffeoylquinic acids
Moderate antioxidant effects
(11th position among 23 herbal
products)
Antiinflammatory
effects
In vitro and in vivo (at
least 4 rat studies)
Triterpenes, leiocarposide,
rutin and quercetin,
caffeoylquinic derivatives
Effect not superior to
conventional NSAIDs
(phenylbutazone,
indomethacin)
Analgesic activity In vivo (one study in
mice) Leiocarposide
Similar effects to
aminophenazone, but very
short duration (one hour)
Spasmolytic activity In vitro or ex vivo NA
Modest spasmolytic effect (less
than 15% of the papaverine
effect)
Antihypertensive
activity
In vivo (one study in
dogs and one in rats)
Antihypertensive activity
attributed to flavonols
Contradictory results on blood
pressure
Diuretic effects In vitro, in vivo (at least
three rat studies)
Flavonoid fraction (quercetin
and its derivatives),
hydroxycinnamic acid fraction,
saponin fraction, leiocarposide
Effects comparable to those of
furosemide or slightly inferior
for several fractions
Control of overactive
bladder symptoms
In vitro data showing
antimuscarinic effects
and two clinical, open,
un-controlled data
NA
Low quality, but promising
data. Contradictory findings
between reduction of urination
frequency seen in clinical data
and diuretic effects reported in
non-clinical studies
Antilithiatic effects One rat study Leiocarposide Additional confirmation
needed
Antibacterial effects In vitro data only
Matricaria ester isomers,
clerodane diterpenes (but their
effects are rather modest)
Modest effects in the majority
of studies. One disc diffusion
study reported encouraging
results on S. aureus and
E. fecalis for a methanol extract.
Biomolecules 2020,10, 1619 23 of 31
Table 3. Cont.
Pharmacological
Properties Evidence
Chemical Compounds/
Fraction to Which the
Property is Ascribed
Critical Assessment of Study
Results
Antifungal activity In vitro data only Triterpene saponins
Modest effects for most fungi
tested up to date. Inhibition of
inhibition of Candida biofilm
formation and of yeast-hyphal
switch seems more promising,
but the clinical relevance is
unclear.
Antiparasite activity
One in vivo study for
Acanthamoeba, one
in vitro study for
Haemonchus contortus
NA
Promising results for
Acanthamoeba (additional
confirmation needed).
No effect against H. contortus
Cytotoxic and
antitumor activity
Mostly in vitro data,
one in vivo study (mice)
Saponins, α-tocopherol
quinone, 2-phyten-l-ol,
a protein, β–dictyopterol,
methyl 3,5-dicaffeoyl quinate
Moderate effects against some
cancer cell lines.
More research needed.
Antimutagenic activity In vitro (one study) Hexane soluble fraction
Effects observed at a quite high
concentration (2.5 mg/mL).
Antiadipogenic effects
In vitro (two studies)
and in vivo (one murine
study)
5-di-O-caffeoylquinic acid
Promising results, further data
necessary
Antidiabetic effects One rat model Hydro-alcoholic fraction More data necessary
Cardioprotective
effects One rat model NA More data necessary
Antisenescence effects One in vitro study NA More data necessary
7. Conclusions
S. virgaurea L. is a native species in Europe, with a long tradition of medicinal use for a variety
of therapeutic purposes in different geographical reasons: urinary tract conditions, gastro-intestinal
conditions, diabetes, allergies, as well as for healing and antiseptic purposes. The largest body of
research has been limited to non-clinical experiments up to date, having a wide variability with
respect to design, methodological quality, and evidence strength. Many of the relevant studies have
been performed before 2000, published in other languages than English, and are not easily accessible
today. Clinical investigation has been very limited in scope and volume, and the little clinical data
available are not of the highest quality (no clinical randomized, controlled, double-blind study has
been published thus far). Whereas some of the pharmacological activities have not been promising
(e.g., the antibacterial, antifungal, or cytotoxic effects seem rather modest), others seem more interesting
and invite to further research (e.g., antiadipogenic, antisenescence, or the beneficial effects in dysuria
and overactive/irritable bladder).
Author Contributions:
Conceptualization, T.C. and R.A.; methodology R.A. and M.D.; validation, C.F., L.U.,
and M.D.; formal analysis, R.A. and L.U.; investigation, C.F. and R.A.; writing—original draft preparation, C.F.
and R.A.; writing—review and editing, T.C., L.U., M.D. and R.A.; visualization, R.A.; supervision, T.C. and M.D.
All authors have read and agreed to the published version of the manuscript.
Funding:
This paper was financially supported by Carol Davila University of Medicine and Pharmacy through
Contract No. CNFIS-FDI-2020-0604 (MEDEX-III) funded by the Ministry of Education and Research, Romania,
from the Institutional Development Fund for Public Universities—FDI 2020.
Acknowledgments:
In this section you can acknowledge any support given which is not covered by the author
contribution or funding sections. This may include administrative and technical support, or donations in kind
(e.g., materials used for experiments).
Conflicts of Interest:
R.A. received consultancy or speakers’ fees from UCB, Sandoz, Abbvie, Zentiva, Teva,
Laropharm, CEGEDIM, Angelini, Biessen Pharma, Hofigal, AstraZeneca, and Stada. All other authors report no
conflict of interest.
Biomolecules 2020,10, 1619 24 of 31
References
1.
World Flora Online. Consortium WFO (2020): World Flora Online. Available online: http://www.
worldfloraonline.org (accessed on 3 August 2020).
2.
Beck, J.B.; Semple, J.C.; Brull, J.M.; Lance, S.L.; Phillips, M.M.; Hoot, S.B.; Meyer, G.A. Genus-Wide
Microsatellite Primers for the Goldenrods (Solidago; Asteraceae). Appl. Plant Sci.
2014
,2, 1300093. [CrossRef]
[PubMed]
3.
Toiu, A.; Vlase, L.; Vodnar, D.C.; Gheldiu, A.-M.; Oniga, I. Solidago graminifolia L. Salisb. (Asteraceae) as a
Valuable Source of Bioactive Polyphenols: HPLC Profile, In Vitro Antioxidant and Antimicrobial Potential.
Molecules 2019,24, 2666. [CrossRef] [PubMed]
4.
Wo´zniak, D.; ´
Slusarczyk, S.; Domaradzki, K.; Dry´s, A.; Matkowski, A. Comparison of Polyphenol Profile and
Antimutagenic and Antioxidant Activities in Two Species Used as Source of Solidaginis herba—Goldenrod.
Chem. Biodivers. 2018,15, e1800023. [CrossRef] [PubMed]
5.
Goulart, S.; Moritz, M.I.G.; Lang, K.L.; Liz, R.; Schenkel, E.P.; Fröde, T.S. Anti-inflammatory evaluation of
Solidago chilensis Meyen in a murine model of pleurisy. J. Ethnopharmacol.
2007
,113, 346–353. [CrossRef]
[PubMed]
6.
Tutin, T.G.; Heywood, V.H.; Burges, N.A.; Moore, D.M.; Valentine, D.H.; Walters, S.M.; Webb, D.A.
Flora Europaea. Volume 4. Plantaginaceae to Compositae (and Rubiaceae); Zenodo: Geneva, Switzerland, 1976.
7.
Native Plant Trust. Go Botany. Available online: https://gobotany.nativeplanttrust.org/species/euthamia/
graminifolia/(accessed on 1 September 2020).
8.
M
ó
ricz,
Á
.M.; Jamshidi-Aidji, M.; Krüzselyi, D.; Darcsi, A.; Böszörm
é
nyi, A.; Csontos, P.; B
é
ni, S.; Ott, P.G.;
Morlock, G.E. Distinction and valorization of 30 root extracts of five goldenrod (Solidago) species. J. Chromatogr.
A2020,1611, 460602. [CrossRef]
9.
European Medicines Agency. Assessment Report on Solidago virgaurea L., Herba; European Medicines Agency:
London, UK, 2008.
10.
Abdel Motaal, A.; Ezzat, S.M.; Tadros, M.G.; El-Askary, H.I.
In vivo
anti-inflammatory activity of
caffeoylquinic acid derivatives from Solidago virgaurea in rats. Pharm. Biol. 2016,54, 2864–2870. [CrossRef]
11. Yarnell, E. Botanical medicines for the urinary tract. World J. Urol. 2002,20, 285–293. [CrossRef]
12.
Cai, T.; Caola, I.; Tessarolo, F.; Piccoli, F.; D’Elia, C.; Caciagli, P.; Nollo, G.; Malossini, G.; Nesi, G.;
Mazzoli, S.; et al. Solidago, orthosiphon, birch and cranberry extracts can decrease microbial colonization and
biofilm development in indwelling urinary catheter: A microbiologic and ultrastructural pilot study. World J.
Urol. 2014,32, 1007–1014. [CrossRef]
13.
Calalb, T.; Fursenco, C.; Ionita, O.; Ghendov, V. The morpho-anatomical study of Solidago virgaurea L. species
from the flora of Republic of Moldova. East Eur. Sci. J. 2018,2-1, 4–13.
14.
Szymura, M.; Wolski, K. Leaf Epidermis Traits as Tools to Identify Solidago L. Taxa in Poland. Acta Biol. Crac.
Ser. Bot. 2011,53. [CrossRef]
15.
Dobjanschi, L.; Vostinaru, O.; Tamas, M. Criteria for Solidago Species Differentiation. Acta Univ. Cibiniensis
Ser. F Chem. 2005,8, 41–44.
16.
Buynov, M.; Goryachkina, Y.; Fedoseyeva, G. Otlichitel’nyye Morfologicheskiye i Anatomo-Diagnosticheskiye
Priznaki Vidov Zolotarnikov—Kanadskogo, Obyknovennogo, Daurskogo: Uchebnoye Posobiye; IGMU: Irkutsk,
Russia, 2013.
17.
Souza, D.M.F.D.; S
á
, R.D.; Ara
ú
jo, E.L.; Randau, K.P. Anatomical, phytochemical and histochemical study of
Solidago chilensis Meyen. An. Acad. Bras. Ciênc. 2018,90, 2107–2120. [CrossRef]
18.
Fedotova, V.; Chelombit’sh, V. Anatomo-diagnosticheskoye izucheniye zolotarnika kavkazskogo (Solidago
caucasica Kem.-Nath.) flory Severnogo Kavkaza. In Razrabotka, Issledovaniye i Marketing Novoy
Farmatsevticheskoy Produktsii: Sb. nauch. tr.; Vyp. 67; The Publisher of Medicine and Pharmacy Institute of
Pyatigorsk: Pyatigorsk, Russia, 2012.
19.
Bley, L.F. Ueber Verwechselung der herba Solidaginis virgaureae mit herba Senecion. nemorensis. Arch. Pharm.
1850,113, 34–37. [CrossRef]
20.
Semple, J. An intuitive phylogeny and summary of chromosome number variation in the goldenrod genus
Solidago (Asteraceae: Astereae). Phytoneuron 2016,32, 1–9.
Biomolecules 2020,10, 1619 25 of 31
21.
Sakaguchi, S.; Kimura, T.; Kyan, R.; Maki, M.; Nishino, T.; Ishikawa, N.; Nagano, A.J.; Honjo, M.N.;
Yasugi, M.; Kudoh, H.; et al. Phylogeographic analysis of the East Asian goldenrod (Solidago virgaurea
complex, Asteraceae) reveals hidden ecological diversification with recurrent formation of ecotypes. Ann. Bot.
2018,121, 489–500. [CrossRef]
22.
Kiełtyk, P.; Mirek, Z. Taxonomy of the Solidago virgaurea Group (Asteraceae) in Poland, with Special Reference
to Variability along an Altitudinal Gradient. Folia Geobot. 2014,49, 259–282. [CrossRef]
23. Yuzepchuk, S. Zolotarnik–Solidago L. In Flora SSSR 25; Shishkin, B., Ed.; Izdatel’stvo Akademii Nauk SSSR:
Moscow, Russia; Saint Petersburg, Russia, 1959; pp. 31–50.
24.
Slav
í
k, B. Solidago L.–zlatob
ý
l. In Kvˇetena ˇ
Cesk
é
Republiky 7 (Flora of the Czech Republic 7); Slav
í
k, B.,
Štˇepánková, J., Eds.; Academia: Prague, Czech Republic, 2004; pp. 114–123.
25.
Botanical Society of Britain and Ireland; Biological Records Center; UK Center for Ecology and Hydrology;
Joint Nature Conservation Committee. Online Atlas of the British and Irish Flora—Solidago virgaurea.
Available online: https://www.brc.ac.uk/plantatlas/plant/solidago-virgaurea (accessed on 28 August 2020).
26.
Sakaguchi, S.; Horie, K.; Ishikawa, N.; Nagano, A.J.; Yasugi, M.; Kudoh, H.; Ito, M. Simultaneous evaluation
of the effects of geographic, environmental and temporal isolation in ecotypic populations of Solidago
virgaurea.New Phytol. 2017,216, 1268–1280. [CrossRef]
27.
Laphitz, R.M.L.; Semple, J.C. A Multivariate Morphometric Analysis of the Solidago chilensis Group in South
America and Related Taxa in North America (Asteraceae, Astereae) 1. Ann. Mo. Bot. Gard.
2015
,100, 423–441.
[CrossRef]
28.
American Botanical Council. Solidago virgaurea L. Available online: http://cms.herbalgram.org/MedPlantID/
BotanicalEntries/Solidago_virgaurea.html (accessed on 28 August 2020).
29.
European Medicines Agency. Community Herbal Monograph on Solidago virgaurea L. Herba, EMEA/HMPC/
285758/2007 2008. Available online: https://www.ema.europa.eu/en/documents/herbal-monograph/final-
community-herbal-monograph-solidago-virgaurea-l-herba_en.pdf (accessed on 28 August 2020).
30. Blum, H. Goldenrod has increasing importance as a herbal drug. Gemuse 1999,35, 431–432.
31.
Borchert, V.E.; Czyborra, P.; Fetscher, C.; Goepel, M.; Michel, M.C. Extracts from Rhois aromatica and
Solidaginis virgaurea inhibit rat and human bladder contraction. Naunyn-Schmiedeberg’s Arch. Pharmacol.
2004,369, 281–286. [CrossRef]
32.
M
ó
ricz,
Á
.M.; Ott, P.G.; Häbe, T.T.; Darcsi, A.; Böszörm
é
nyi, A.; Alberti,
Á
.; Krüzselyi, D.; Csontos, P.;
B
é
ni, S.; Morlock, G.E. Effect-Directed Discovery of Bioactive Compounds Followed by Highly Targeted
Characterization, Isolation and Identification, Exemplarily Shown for Solidago virgaurea.Anal. Chem.
2016
,
88, 8202–8209. [CrossRef]
33.
Melzig, M.F. Goldenrod—A classical exponent in the urological phytotherapy. Wien. Med. Wochenschr.
2004
,
154, 523–527. [CrossRef]
34.
Gross, S.C.; Goodarzi, G.; Watabe, M.; Bandyopadhyay, S.; Pai, S.K.; Watabe, K. Antineoplastic activity of
Solidago virgaurea on prostatic tumor cells in an SCID mouse model. Nutr. Cancer
2002
,43, 76–81. [CrossRef]
35.
Tkachev, A.V.; Korolyuk, E.A.; Letchamo, W. Volatile Oil-Bearing Flora of Siberia VIII: Essential Oil
Composition and Antimicrobial Activity of Wild Solidago virgaurea L. from the Russian Altai. J. Essent. Oil Res.
2006,18, 46–50. [CrossRef]
36.
Heger, M. Entzündliche Erkrankungen der Urogenitalorgane. In Naturheilverfahren in der Urologie; Springer:
Berlin/Heidelberg, Germany, 2003; pp. 55–102. ISBN 978-3-662-08920-0.
37.
Bader, G.; Wray, V.; Hiller, K. The Main Saponins from the Aerial Parts and the Roots of Solidago virgaurea
subsp. virgaurea *. Planta Med. 1995,61, 158–161. [CrossRef]
38.
A. Vogel. Plant Encylopedia Solidago virgaurea L. Available online: https://www.avogel.com/plant-
encyclopaedia/solidago_virgaurea.php (accessed on 28 August 2020).
39.
Tom
á
nkov
á
, V.; Vlˇckov
á
, A.; Anzenbacher, P.; Bachleda, P.; Anzenbacherov
á
, E. Už
í
v
á
n
í
pˇr
í
pravk˚u ze
zlatob
ý
lu obecn
é
ho (Solidago virgaurea) neovlivˇnuje metabolizmus souˇcasn ˇe pod
á
van
ý
ch l
é
ˇciv. Klin Farm.
Farm 2016,30, 11–15.
40.
Nawło´c pospolita (Solidago virgaurea). Available online: https://www.plantago-sklep.pl/blog/nawloc-
pospolita/(accessed on 28 August 2020).
41.
Malankina, Y. Zolotarnik: Lekarstvennyye svoystva i primeneniye. Available online: https://www.greeninfo.
ru/grassy/solidago_canadensis.html/Article/_/aID/5959 (accessed on 28 August 2020).
Biomolecules 2020,10, 1619 26 of 31
42.
Suleymanova, F.S.; Nesterova, O.V. The historical background and prospects of Canadian goldenrod
(Solidago canadensis L.) herb medicinal use. J. Sci. Artic. Health Educ. Millenn. 2017,19, 142–149. [CrossRef]
43.
Kolosova, V. Name–Text–Ritual: The Role of Plant Characteristics in Slavic Folk Medicine. Folklorika
2005
,X,
44–61. [CrossRef]
44.
Kiselova, Y.; Ivanova, D.; Chervenkov, T.; Gerova, D.; Galunska, B.; Yankova, T. Correlation between theIn
Vitro antioxidant activity and polyphenol content of aqueous extracts from bulgarian herbs. Phytother. Res.
2006,20, 961–965. [CrossRef]
45.
Dobjanschi, L.; Paltinean, R.; Vlase, L.; Babota, M.; Fritea, L.; Tamas, M. Comparative phytochemical research
of Solidago genus: S. graminifolia. Note I. Flavonoids. Acta Biol. Marisiensis 2018,1, 18–26.
46. Calalb, T.; Bodrug, M. Botanica Faramaceutică; CEP Medicina: Chisinau, Moldova, 2009.
47. Tamas, M.; Toader, S. Ac¸tiunea diureticăa unor specii de Solidago.Clujul Med. 1989,62, 75–79.
48.
Petran, M.; Dragos, D.; Gilca, M. Historical ethnobotanical review of medicinal plants used to treat children
diseases in Romania (1860s–1970s). J. Ethnobiol. Ethnomed. 2020,16, 15. [CrossRef]
49.
Choi, S.Z.; Choi, S.U.; Lee, K.R. Pytochemical constituents of the aerial parts from Solidago virga-aurea var.
gigantea. Arch. Pharmacal. Res. 2004,27, 164–168. [CrossRef]
50.
Sung, J.H.; Lee, J.O.; Son, J.K.; Park, N.S.; Kim, M.R.; Kim, J.G.; Moon, D.C. Cytotoxic constituents from
Solidago virga-aurea var. gigantea MIQ. Arch. Pharmacal. Res. 1999,22, 633–637. [CrossRef]
51.
Inose, Y.; Miyase, T.; Ueno, A. Studies on the Constituents of Solidago virga-aurea L. I. Structural Elucidation
of Saponins in the Herb. Chem. Pharm. Bull. 1991,39, 2037–2042. [CrossRef]
52.
Dmuchowski, W.; Gozdowski, D.; Br ˛agoszewska, P.; Baczewska, A.H.; Suwara, I. Phytoremediation of zinc
contaminated soils using silver birch (Betula pendula Roth). Ecol. Eng. 2014,71, 32–35. [CrossRef]
53.
Bielecka, A.; Kr
ó
lak, E. The accumulation of Mn and Cu in the morphological parts of Solidago canadensis
under different soil conditions. PeerJ 2019,7, e8175. [CrossRef]
54.
Bielecka, A.; Kr
ó
lak, E. Solidago canadensis as a bioaccumulator and phytoremediator of Pb and Zn. Environ. Sci.
Pollut Res. Int. 2019,26, 36942–36951. [CrossRef]
55. Oldroyd, B.P. What’s Killing American Honey Bees? PLoS Biol. 2007,5, e168. [CrossRef]
56.
Metzner, J.; Hirschelmann, R.; Hiller, K. Antiphlogistic and analgesic effects of leiocarposide, a phenolic
bisglucoside of Solidago virgaurea L. Pharmazie 1984,39, 869–870.
57.
Chodera, A.; Dabrowska, K.; Bobkiewicz-Kozłowska, T.; Tkaczyk, J.; Skrzypczak, L.; Budzianowski, J. Effect
of leiocarposide on experimental urinary calculi in rats. Acta Pol. Pharm. 1988,45, 181–186. [PubMed]
58.
Thiem, B.; Wesołowska, M.; Skrzypczak, L.; Budzianowski, J. Phenolic compounds in two Solidago L. species
from in vitro culture. Acta Pol. Pharm. 2001,58, 277–281. [PubMed]
59.
Roslon, W.; Osinska, E.; Mazur, K.; Geszprych, A. Chemical characteristics of European goldenrod (Solidago
virgaurea L. subsp. virgaurea) from natural sites in central and Eastern Poland. Acta Sci. Pol. Hortorum Cultus
2014,13, 55–65.
60.
Borkowski, B.; Skrzypczakowa, L. Polyphenolic Compounds in Herbs of the Species Solidago L. Acta Pol.
Pharm. 1962,19, 491–495.
61.
Budzianowski, J.; Skrzypczak, L.; Wesolowska, M. Flavonoid patterns in the genus Solidago.Pharm. Weekbl.
Method. 1987,9, 230.
62.
Pietta, P.; Gardana, C.; Mauri, P.; Zecca, L. High-performance liquid chromatographic analysis of flavonol
glycosides of Solidago virgaurea.J. Chromatogr. A 1991,558, 296–301. [CrossRef]
63. Tamas, M. Cercetări chemotaxonomice la genul Solidago.Contrib. Bot. (Cluj-Napoca) 1986, 110–113.
64.
Dobjanschi, L.; Fritea, L.; Patay, E.B.; Tamas, M. Comparative study of the morphological and phytochemical
characterization of Romanian Solidago species. Pak. J. Pharm. Sci. 2019,32, 1571–1579.
65.
Chodera, A.; Dabrowska, K.; Sloderbach, A.; Skrzypczak, L.; Budzianowski, J. Effect of flavonoid fractions of
Solidago virgaurea L. on diuresis and levels of electrolytes. Acta Pol. Pharm. 1991,48, 35–37.
66.
Bader, G.; Binder, K.; Hiller, K.; Ziegler-Böhme, H. The antifungal action of triterpene saponins of Solidago
virgaurea L. Die Pharm. 1987,42, 140.
67.
Bader, G.; Kulhanek, Y.; Ziegler-Böhme, H. Zur antimyzetischen Wirksamkeit von polygalasäureglycosiden.
Pharmazie 1990,45, 618–620. [PubMed]
Biomolecules 2020,10, 1619 27 of 31
68.
Bader, G.; Plohmann, B.; Franz, G. Saponin from Solidago virgaurea L. Possible Agents for Therapy of Cancer?
In Proceedings of the 44th Annual Congress of the Society for Medicinal Plant Research and a Joint Meeting
with the Czech Biotechnology Society, Prague, Czech Republic, 3–7 September 1996; Abstracts of Lectures
and Poster Presentations. pp. 3–7.
69.
Bader, G.; Wray, V.; Just, U.; Hiller, K. Enzymatic hydrolysis of the cytotoxic triterpenoid glycoside
virgaureasaponin 1. Phytochemistry 1998,49, 153–156. [CrossRef]
70.
Inose, Y.; Miyase, T.; Ueno, A. Studies on the Constituents of Solidago virga-aurea L. II. Structures of
Solidagosaponins X-XX. Chem. Pharm. Bull. 1992,40, 946–953. [CrossRef]
71.
Dobjanschi, L.; Zdrinca, M.; Muresan, M.; Vicas, S.; Antonescu, A. The thin layer chromatography analysis of
saponins belonging to Solidago species. Fasc. Prot. Mediu. 2013,21, 56–60.
72.
Chevalier, M.; Medioni, E.; Pr
ê
cheur, I. Inhibition of Candida albicans yeast–hyphal transition and biofilm
formation by Solidago virgaurea water extracts. J. Med. Microbiol. 2012,61, 1016–1022. [CrossRef]
73.
Laurençon, L.; Sarrazin, E.; Chevalier, M.; Pr
ê
cheur, I.; Herbette, G.; Fernandez, X. Triterpenoid saponins
from the aerial parts of Solidago virgaurea alpestris with inhibiting activity of Candida albicans yeast-hyphal
conversion. Phytochemistry 2013,86, 103–111. [CrossRef]
74.
Kalemba, D. Constituents of the essential oil of Solidago virgaurea L. Flavour Fragr. J.
1998
,13, 373–376.
[CrossRef]
75.
Kalemba, D.; Thiem, B. Constituents of the essential oils of four micropropagated Solidago species. Flavour
Fragr. J. 2004,19, 40–43. [CrossRef]
76.
Fujita, Y.; Fujita, S.; Hayama, Y. Miscellaneous contributions to the essential oils of plants from various
territories. Components of the essential oils of Solidago virgaurea Linn. ssp. Nippon Nogei Kagaku Kaishi
1990
,
64, 1729–1732. (In Japanese) [CrossRef]
77.
Bertoli, A.; Cioni, P.L.; Flamini, G.; Morelli, I.; Spinelli, G.; Tomei, P.E. Constituents of the Essential Oil
of Solidago litoralis, an Endemic Plant from Northern Tuscany (Italy). J. Essent. Oil Res.
1999
,11, 215–216.
[CrossRef]
78.
Starks, C.M.; Williams, R.B.; Goering, M.G.; O’Neil-Johnson, M.; Norman, V.L.; Hu, J.-F.; Garo, E.;
Hough, G.W.; Rice, S.M.; Eldridge, G.R. Antibacterial clerodane diterpenes from Goldenrod (Solidago virgaurea).
Phytochemistry 2010,71, 104–109. [CrossRef]
79.
Pychenkova, P.A. Dynamics of the amount and characteristics of the polysaccharides of Solidago virgaurea.
Chem. Nat. Compd. 1987,23, 246–247. [CrossRef]
80.
Lam, J. Polyacetylenes of Solidago virgaurea: Their seasonal variation and NMR long-range spin coupling
constants. Phytochemistry 1971,10, 647–653. [CrossRef]
81.
Council of Europe. European Pharmacopoeia (Ph. Eur.), 10th ed.; Council of Europe: Strasbourg, France, 2019.
82.
Fuchs, L. Isolierung Von Quercitrin Aus Solidago Virga Aurea L., S. Serotina Ait und S. Canadensis L. Sci. Pharm.
1949,17, 128–131. [PubMed]
83.
Ap
á
ti, P.; Szentmih
á
lyi, K.; Krist
ó
, S.T.; Papp, I.; Vinkler, P.; Szoke,
É
.; K
é
ry,
Á
. Herbal remedies of
Solidago—correlation of phytochemical characteristics and antioxidative properties. J. Pharm. Biomed. Anal.
2003,32, 1045–1053. [CrossRef]
84.
Kraujalien
˙
e, V.; Pukalskas, A.; Venskutonis, P.R. Biorefining of goldenrod (Solidago virgaurea L.) leaves by
supercritical fluid and pressurized liquid extraction and evaluation of antioxidant properties and main
phytochemicals in the fractions and plant material. J. Funct. Foods 2017,37, 200–208. [CrossRef]
85. Kalemba, D. Phenolic acids in four Solidago species. Pharmazie 1992,47, 471–472.
86.
Bajkacz, S.; Baranowska, I.; Buszewski, B.; Kowalski, B.; Ligor, M. Determination of Flavonoids and Phenolic
Acids in Plant Materials Using SLE-SPE-UHPLC-MS/MS Method. Food Anal. Methods
2018
,11, 3563–3575.
[CrossRef]
87.
Haghi, G.; Hatami, A. Simultaneous Quantification of Flavonoids and Phenolic Acids in Plant Materials by a
Newly Developed Isocratic High-Performance Liquid Chromatography Approach. J. Agric. Food Chem.
2010
,
58, 10812–10816. [CrossRef]
88.
Marksa, M.; Zymone, K.; Ivanauskas, L.; Radušien
˙
e, J.; Pukalskas, A.; Raudone, L. Antioxidant profiles of
leaves and inflorescences of native, invasive and hybrid Solidago species. Ind. Crops Prod.
2020
,145, 112123.
[CrossRef]
89.
Fraisse, D.; Felgines, C.; Texier, O.; Lamaison, J.-L. Caffeoyl Derivatives: Major Antioxidant Compounds of
Some Wild Herbs of the Asteraceae Family. Food Nutr. Sci. 2011,02, 181–192. [CrossRef]
Biomolecules 2020,10, 1619 28 of 31
90.
Jaiswal, R.; Kiprotich, J.; Kuhnert, N. Determination of the hydroxycinnamate profile of 12 members of the
Asteraceae family. Phytochemistry 2011,72, 781–790. [CrossRef] [PubMed]
91.
Dobi
á
š, P.; Pavl
í
kov
á
, P.; Adam, M.; Eisner, A.; Beˇnov
á
, B.; Ventura, K. Comparison of pressurised fluid and
ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity. Open Chem.
2010,8, 87–95. [CrossRef]
92.
Goswami, A.; Barua, R.N.; Sharma, R.P.; Baruah, J.N.; Kulanthaivel, P.; Herz, W. Clerodanes from Solidago
virgaurea.Phytochemistry 1984,23, 837–841. [CrossRef]
93.
Melzig, M.; Löser, B.; Bader,G.; Papsdorf, G. European goldenrod as an anti-inflammatory drug: Investigations
into the cyto-and molecular pharmacology for a better understanding of the anti-inflammatory activity of
preparations from Solidago virgaurea.Z. Phytother. 2000,21, 67–70.
94.
Vuolo, M.M.; Lima, V.S.; Junior, M.R.M. Phenolic compounds: Structure, classification, and antioxidant
power. In Bioactive Compounds; Elsevier: Amsterdam, The Netherlands, 2019; pp. 33–50.
95.
Demir, H.; Acik, L.; Bali, E.B.; Koç, L.Y.; Kaynak, G. Antioxidant and antimicrobial activities of Solidago
virgaurea extracts. Afr. J. Biotechnol. 2009,8, 2.
96.
Meyer, B.; Schneider, W.; Elstner, E. Antioxidative properties of alcoholic extracts from Fraxinus excelsior,
Populus tremula and Solidago virgaurea.Arzneimittel-forschung 1995,45, 174. [PubMed]
97.
Duarte, J.; Utrilla, P.; Jimenez, J.; Tamargo, J.; Zarzuelo, A.; P
é
rez Vizca
í
no, F. Vasodilatory effects of flavonoids
in rat aortic smooth muscle. Structure-activity relationships. Gen. Pharmacol. 1993,24, 857–862. [CrossRef]
98.
Kruk, J.; Baranowska, I.; Buszewski, B.; Bajkacz, S.; Kowalski, B.; Ligor, M. Flavonoids enantiomer distribution
in different parts of goldenrod (Solidago virgaurea L.), lucerne (Medicago sativa L.) and phacelia (Phacelia
tanacetifolia Benth.). Chirality 2019,31, 138–149. [CrossRef]
99.
Bjorkman, O.; Holmgren, P. Preliminary Observations on Anthocyanins and Other Flavonoid Compounds
and Respiration Rates in Different Ecotypes of Solidago virgaurea.Physiol. Plant.
1958
,11, 154–157. [CrossRef]
100.
Jang, Y.; Wang, Z.; Lee, J.-M.; Lee, J.-Y.; Lim, S. Screening of Korean Natural Products for Anti-Adipogenesis
Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from
Solidago virgaurea.Molecules 2016,21, 226. [CrossRef] [PubMed]
101.
Hiller, K.; Dube, G.; Zeigan, D. Virgaureoside aa new bisdesmosidic phenol glycoside from Solidago virgaurea.
Die Pharm. 1985,40, 795–796.
102.
Paun, G.; Neagu, E.; Albu, C.; Radu, G.L. Verbascum phlomoides and Solidago virgaureae herbs as natural
source for preventing neurodegenerative diseases. J. Herb. Med. 2016,6, 180–186. [CrossRef]
103.
Skrzypczak, L.; Wesołowska, M.; Thiem, B.; Budzianowski, J. Solidago L. Species (Goldenrod): In Vitro
Regeneration and Biologically Active Secondary Metabolites. In Medicinal and Aromatic Plants XI; Bajaj, Y.P.S.,
Ed.; Biotechnology in Agriculture and Forestry; Springer: Berlin/Heidelberg, Germany, 1999; Volume 43,
pp. 384–403. ISBN 978-3-642-08363-1.
104.
Bader, G.; Janka, M.; Hannig, H.; Hiller, K. Zur quantitativen Bestimmung von Leiocaposid in Solidago
virgaurea L. Die Pharm. 1990,45, 380–381.
105.
Nkuimi Wandjou, J.G.; Quassinti, L.; Gudžinskas, Z.; Nagy, D.U.; Cianfaglione, K.; Bramucci, M.; Maggi, F.
Chemical composition and antiproliferative effect of essential oils of four Solidago species (S. canadensis,
S. gigantea,S. virgaurea and S.×niederederi). Chem. Biodivers. 2020. [CrossRef]
106.
Hiller, K.; Genzel, S.; Murach, M.; Franke, P. Zur Kenntnis der Saponine der Gattung Solidago. 1 Mitteilung:
Uber die Saponine von Solidago virgaurea L. Pharmazie 1975,30, 188–190. [PubMed]
107.
Bader, G.; Wray, V.; Hiller, K. Virgaureasaponin 3, a 3,28-bisdesmosidic triterpenoid saponin from Solidago
virgaurea.Phytochemistry 1992,31, 621–623. [CrossRef]
108.
Miyase, T.; Inose, Y.; Ueno, A. Studies on the Constituents of Solidago virga-aurea L. III. Structures of
Solidagosaponins XXI-XXIX. Chem. Pharm. Bull. 1994,42, 617–624. [CrossRef]
109.
Schöpke, T.; Wray, V.; Rzazewska, B.; Hiller, K. Bellissaponins BA1 and BA2, acylated saponins from Bellis
perennis.Phytochemistry 1991,30, 627–631. [CrossRef]
110.
Saluk-Juszczak, J.; Pawlaczyk, I.; Olas, B.; Kołodziejczyk, J.; Ponczek, M.; Nowak, P.; Tsirigotis-Wołoszczak, M.;
Wachowicz, B.; Gancarz, R. The effect of polyphenolic-polysaccharide conjugates from selected medicinal
plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins. Int. J. Biol.
Macromol. 2010,47, 700–705. [CrossRef] [PubMed]
111.
Salehi, B.; Martorell, M.; Arbiser, J.L.; Sureda, A.; Martins, N.; Maurya, P.K.; Sharifi-Rad, M.; Kumar, P.;
Sharifi-Rad, J. Antioxidants: Positive or Negative Actors? Biomolecules 2018,8, 124. [CrossRef] [PubMed]
Biomolecules 2020,10, 1619 29 of 31
112.
Woo, J.H.; Shin, S.L.; Jeong, H.S.; Lee, C.H. Influence of Applied Pressure and Heat Treatment on Antioxidant
Activities of Young Leaves from Achillea alpina and Solidago virgaurea subsp. gigantea. Korean J. Plant Res.
2010,23, 123–130.
113.
Kim, Y.-J.; Kim, H.-Y.; Choe, J.-H.; Park, J.-H.; Ham, Y.-K.; Yeo, E.-J.; Hwang, K.-E.; Kim, C.-J. Antioxidant
activity of goldenrod (Solidago virgaurea) leaf and stem powder on raw ground pork during chilled storage.
Korean J. Food Sci. Anim. Resour. 2013,33, 1–8. [CrossRef]
114.
Choe, J.; Choi, Y.; Kim, H.; Han, D.; Kim, H.; Kim, Y.; Park, J.; Chung, H.; Kim, C. Effects of goldenrod
(Solidago virgaurea) leaf and stem extracts on oxidative stability in cooked ground pork during chilled
storage. In Proceedings of the International Congress of Meat Science and Technology, Ghent, Belgium,
7–12 August 2011; pp. 7–12.
115.
Jacker, H.; Voigt, G.; Hiller, K. Zum antiexsudativen verhalten einiger triterpensaponine. Pharmazie
1982
,
37, 380–382.
116.
Choi, S.Z.; Choi, S.U.; Bae, S.Y.; Neung Pyo, S.; Lee, K.R. Immunobioloical activity of a new benzyl benzoate
from the aerial parts of Solidago virga-aurea var. gigantea.Arch. Pharmacal. Res. 2005,28, 49. [CrossRef]
117.
El-Ghazaly, M.; Khayyal, M.; Okpanyi, S.; Arens-Corell, M. Study of the anti-inflammatory activity of Populus
tremula,Solidago virgaurea and Fraxinus excelsior.Arzneimittel-forschung 1992,42, 333–336.
118.
Strehl, E.; Schneider, W.; Elstner, E.F. Inhibition of dihydrofolate reductase activity by alcoholic extracts from
Fraxinus excelsior,Populus tremula and Solidago virgaurea.Arzneimittel-forschung 1995,45, 172–173.
119.
ESCOP Solidago virgaurea. In Monographs on the Medicinal Uses of Plant Drugs; European Scientific
Cooperative on Phytotherapy Thieme: Exeter, UK; Stuttgart, Germany; New York, NY, USA, 2003; pp. 487–491.
ISBN 1-58890-233-1.
120.
Schweitzer, B.I.; Dicker, A.P.; Bertino, J.R. Dihydrofolate reductase as a therapeutic target. FASEB J.
1990
,4,
2441–2452. [CrossRef]
121.
Bonaterra, G.A.; Schwarzbach, H.; Kelber, O.; Weiser, D.; Kinscherf, R. Anti-inflammatory effects of
Phytodolor
®
(STW 1) and components (poplar, ash and goldenrod) on human monocytes/macrophages.
Phytomedicine 2019,58, 152868. [CrossRef]
122.
Von Kruedener, S.; Schneider, W.; Elstner, E. A combination of Populus tremula,Solidago virgaurea and Fraxinus
excelsior as an anti-inflammatory and antirheumatic drug. A short review. Arzneimittel-forschung
1995
,
45, 169–171.
123.
Sampson, J.H.; Phillipson, J.D.; Bowery, N.G.; O’Neill, M.J.; Houston, J.G.; Lewis, J.A. Ethnomedicinally
selected plants as sources of potential analgesic compounds: Indication of
in vitro
biological activity in
receptor binding assays. Phytother. Res. 2000,14, 24–29. [CrossRef]
124.
Westendorf, J.; Vahlensieck, W. Spasmolytische und kontraktile Einflüsse eines pflanzlichen
Kombinations-präparates auf die glatte Muskulatur des isolierten Meerschweinchendarms.
Arzneimittel-forschung/Drug Res. 1981,31, 40–43.
125.
R
á
cz, G.; R
á
cz-Kotilla, E.; J
ó
zsa, J. Hypotensive Activity-A Possible Pharmacotaxonomic Character of Solidago
L. In Proceedings of the II International Symposium on Spices and Medicinal Plants, Budapest, Hungary,
16–22 July 1979; Volume 96, pp. 15–18.
126.
Raczkotilla, E.; Racz, G. Hypotensive and sedative effect of extracts obtained from Soldidago virgaurea L.
[golden rod, drug plants]. Planta Med. 1978,33, 300.
127.
Lasserre, B.; Kaiser, R.; Chanh, P.H.; Ifansyah, N.; Gleye, J.; Moulis, C. Effects on rats of aqueous extracts of
plants used in folk medicine as antihypertensive agents. Naturwissenschaften
1983
,70, 95–96. [CrossRef]
[PubMed]
128.
Mangiafico, S.; Costello-Boerrigter, L.C.; Andersen, I.A.; Cataliotti, A.; Burnett, J.C. Neutral endopeptidase
inhibition and the natriuretic peptide system: An evolving strategy in cardiovascular therapeutics.
Eur. Heart J. 2013,34, 886–893. [CrossRef] [PubMed]
129.
Kaspers, U.; Poetsch, F.; Nahrstedt, A.; Chatterjee, S. Diuretic effects of extracts and fractions obtained from
different Solidago species. Naunyn-Schmiedebergs Arch. Pharmacol. 1998,358, R495.
130.
Chodera, A.; Dabrowska, K.; Se ´nczuk, M.; Wasik-Olejnik, A.; Skrzypczak, L.; Budzianowski, J.;
Ellnain-Wojtaszek, M. Diuretic effect of the glycoside from a plant of the Solidago L. genus. Acta Pol.
Pharm. 1985,42, 199–204.
131.
Chodera, A.; D ˛abrowska, K.; Skrzypczak, L. Biological activity of Leiocarposide from Solidago L. Acta Agron.
Acad. Sci. Hung 1985,34, 112.
Biomolecules 2020,10, 1619 30 of 31
132. Budzianowski, J. Die urologische Wirkung des Leiocarposids. Drogenreport 1999,12, 20–21.
133.
Edwards, S.E.; Rocha, I.; Williamson, E.M.; Heinrich, M. Phytopharmacy: An Evidence-Based Guide to
Herbal Medical Products; Wiley Blackwell: Chichester, West Sussex, UK; Hoboken, NJ, USA, 2015;
ISBN 978-1-118-54345-0.
134.
Kolodziej, B.; Kowalski, R.; K˛edzia, B. Antibacterial and antimutagenic activity of extracts aboveground
parts of three Solidago species: Solidago virgaurea L., Solidago canadensis L. and Solidago gigantea Ait. J. Med.
Plants Res. 2011,5, 6770–6779.
135.
Bussmann, R.W.; Malca-Garc
í
a, G.; Glenn, A.; Sharon, D.; Chait, G.; D
í
az, D.; Pourmand, K.; Jonat, B.;
Somogy, S.; Guardado, G.; et al. Minimum inhibitory concentrations of medicinal plants used in Northern
Peru as antibacterial remedies. J. Ethnopharmacol. 2010,132, 101–108. [CrossRef] [PubMed]
136.
Silva, A.C.O.; Santana, E.F.; Saraiva, A.M.; Coutinho, F.N.; Castro, R.H.A.; Pisciottano, M.N.C.; Amorim, E.L.C.;
Albuquerque, U.P. Which approach is more effective in the selection of plants with antimicrobial activity?
Evid.-Based Complementary Altern. Med. ECAM 2013,2013, 308980. [CrossRef]
137.
Saraiva, M.
In vitro
evaluation of antioxidant, antimicrobial and toxicity properties of extracts of Schinopsis
brasiliensis Engl. (Anacardiaceae). Afr. J. Pharm. Pharmacol. 2011,5, 1724–1731. [CrossRef]
138.
Bueno, J. In Vitro Antimicrobial Activity of Natural Products Using Minimum Inhibitory Concentrations:
Looking for New Chemical Entities or Predicting Clinical Response. Med. Aromat. Plants
2012
,1. [CrossRef]
139.
Thiem, B.; Go´sli´nska, O. Antimicrobial activity of Solidago virgaurea L. from
in vitro
cultures. Fitoterapia
2002
,
73, 514–516. [CrossRef]
140.
Brantner, A.; Grein, E. Antibacterial activity of plant extracts used externally in traditional medicine.
J. Ethnopharmacol. 1994,44, 35–40. [CrossRef]
141.
Brantner, A. Die antimikrobielle Wirkung von Solidago- haltigen Phytourologica. Drogen-Report
1999
,
12, 27–28.
142.
Nair, R.; Kalariya, T.; Chanda, S. Antibacterial Activity of Some Plant Extracts Used in Folk Medicine.
J. Herb. Pharmacother. 2008,7, 191–201. [CrossRef]
143.
Alves, T.M.D.A.; Silva, A.F.; Brand
ã
o, M.; Grandi, T.S.M.; Sm
â
nia, E.D.F.A.; Sm
â
nia J
ú
nior, A.; Zani, C.L.
Biological screening of Brazilian medicinal plants. Mem
ó
rias Do Inst. Oswaldo Cruz
2000
,95, 367–373.
[CrossRef]
144.
Kalemba, D. Constituents and biological activity of the essential oils of some Solidago and Artemisia species.
Sci. Pap. Tech. Univ. Lodz 2000,857, 1–118.
145.
Xie, Y.; Yang, W.; Tang, F.; Chen, X.; Ren, L. Antibacterial activities of flavonoids: Structure-activity
relationship and mechanism. Curr. Med. Chem. 2015,22, 132–149. [CrossRef] [PubMed]
146.
Liu, J.; Du, C.; Beaman, H.T.; Monroe, M.B.B. Characterization of Phenolic Acid Antimicrobial and Antioxidant
Structure–Property Relationships. Pharmaceutics 2020,12, 419. [CrossRef] [PubMed]
147.
Guimar
ã
es, A.C.; Meireles, L.M.; Lemos, M.F.; Guimar
ã
es, M.C.C.; Endringer, D.C.; Fronza, M.; Scherer, R.
Antibacterial Activity of Terpenes and Terpenoids Present in Essential Oils. Molecules
2019
,24, 2471.
[CrossRef]
148.
Wi´nska, K.; M ˛aczka, W.; Łyczko, J.; Grabarczyk, M.; Czubaszek, A.; Szumny, A. Essential Oils as Antimicrobial
Agents—Myth or Real Alternative? Molecules 2019,24, 2130. [CrossRef]
149.
Bader, G.; Seibold, M.; Tintelnot, K.; Hiller, K. Cytotoxicity of triterpenoid saponins. Part 2: Relationships
between the structures of glycosides of polygalacic acid and their activities against pathogenic Candida
species. Pharmazie 2000,55, 72–74.
150.
Pepeljnjak, S.; Kuštrak, D.; Vukuši´c, I. Investigation of the Antimycotic Activities of Solidago virgaurea and
Solidago gigantea Exstracts. In Proceedings of the 45th Annual Congress of the Society for Medicinal Plant
Research, Regensburg, Germany, 7–12 September 1997; Program and Abstracts. p. K18.
151.
Matsuda, H. Studies on chemical structures and pharmacological activities of triterpene saponins. Nat. Med.
1999,53, 223–228.
152.
Derda, M.; Hada´s, E.; Thiem, B. Plant extracts as natural amoebicidal agents. Parasitol. Res.
2009
,104,
705–708. [CrossRef]
153.
V
á
radyov
á
, Z.; Pisarˇc
í
kov
á
, J.; Babj
á
k, M.; Hodges, A.; Mravˇc
á
kov
á
, D.; Kišidayov
á
, S.; Königov
á
, A.;
Vadlejch, J.; V
á
rady, M. Ovicidal and larvicidal activity of extracts from medicinal-plants against Haemonchus
contortus.Exp. Parasitol. 2018,195, 71–77. [CrossRef]
Biomolecules 2020,10, 1619 31 of 31
154.
Plohmann, B.; Bader, G.; Hiller, K.; Franz, G. Immunomodulatory and antitumoral effects of triterpenoid
saponins. Die Pharm. 1997,52, 953–957.
155.
Bader, G.; Plohmann, B.; Hiller, K.; Franz, G. Cytotoxicity of triterpenoid saponins. Part 1: Activities against
tumor cells in vitro and hemolytical index. Pharmazie 1996,51, 414–417.
156.
Hu, W.; Shen, T.; Wang, M.-H. Cell cycle arrest and apoptosis induced by methyl 3,5-dicaffeoyl quinate in
human colon cancer cells: Involvement of the PI3K/Akt and MAP kinase pathways. Chem.-Biol. Interact.
2011,194, 48–57. [CrossRef]
157.
Jang, Y.S.; Kim, H.-Y.; Zuo, G.; Lee, E.H.; Kang, S.K.; Lim, S.S. Constituents from Solidago virgaurea var.
gigantea and their inhibitory effect on lipid accumulation. Fitoterapia 2020,146, 104683. [CrossRef]
158.
Wang, Z.; Kim, J.H.; Jang, Y.S.; Kim, C.H.; Lee, J.-Y.; Lim, S.S. Anti-obesity effect of Solidago virgaurea var. g
igantea extract through regulation of adipogenesis and lipogenesis pathways in high-fat diet-induced obese
mice (C57BL/6N). Food Nutr. Res. 2017,61, 1273479. [CrossRef]
159.
Sanad, F.A.-A.; Ahmed, S.F.; El-Tantawy, W.H. Antidiabetic and hypolipidemic potentials of Solidago virgaurea
extract in alloxan-induced diabetes type 1. Arch. Physiol. Biochem. 2020, 1–8. [CrossRef]
160.
El-Tantawy, W.H. Biochemical effects of Solidago virgaurea extract on experimental cardiotoxicity. J. Physiol.
Biochem. 2014,70, 33–42. [CrossRef]
161.
Lämmermann, I.; Terlecki-Zaniewicz, L.; Weinmüllner, R.; Schosserer, M.; Dellago, H.; de Matos Branco, A.D.;
Autheried, D.; Sevcnikar, B.; Kleissl, L.; Berlin, I.; et al. Blocking negative effects of senescence in human skin
fibroblasts with a plant extract. NPJ Aging Mech. Dis. 2018,4, 1–10. [CrossRef]
Publisher’s Note:
MDPI stays neutral with regard to jurisdictional claims in published maps and institutional
affiliations.
©
2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Content uploaded by Robert Ancuceanu
Author content
All content in this area was uploaded by Robert Ancuceanu on Jan 28, 2021
Content may be subject to copyright.