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Extreme
environment
Dead Sea as a platform for innovative
microbial and biotechnological
research
Colaboradores, lab technician and students
Ashraf AL Ashhab, Rivka ALexander-shani, Yosef Avrahami, Hana Dimeretz,
Danit Parker, Shiri Meshner, Gidon Winters, Shmuel Carmeli and Yael Bar-Lavan
Microbiology of extreme environment
- Thermophilic bacteria (high temperature)
- Halophilic bacteria (high salt)
- Xerophilic bacteria (dry environment)
- Radiation-resistant bacteria
- Polyextremophile bacteria
Microbiology of extreme environment
Our group research activities:
- Classical microbiology
- Next generation sequencing
- Biochemical research collaboration
- Educational activities
Microbiology of extreme environment
Our group research interest are:
- Microbial interaction, physiology and
ecosystem services and evolution in:
1- Plants
2- Soil
3- Water
Up to 50% of bacterial diversity in desert
ecosystems are unclassified
AL Ashhab A. et al., 2019
Tamarix aphylla
Atriplex halimus
Acacia
Desert microbial
library Epiphytes
LB agar plate
93 isolates
Contig Bacterial isolate
Contig 1 25.1
Contig 10 284.2.4, 284.2.5
Contig 1 found to have
99.52% sequence similarity
to Bacillus atrophaeus.
Contig 10 found to have
98.07% sequence similarity
to Bacillus subtilis.
Both are found to be new
isolates.
PCT/62/885,386, 2020
Then, all isolates were tested for against
Botrytis cinerea and Fusarium solani
Fungal Plague 1cm20.25% PDA agarBacterial colony
After 72h
After 1 week
Incubated at 20℃
10 ul of OD 0.5
PCT/62/885,386, 2020
SWITCH comercial formula Isolate 284.2.8
PCT/62/885,386, 2020
Isolates 25.5 and
284.2.9 inhibit
Botrytis cerena in
pepper whole plant
experiment
Pepper plants were wounded
by decapitation, then 20 µl of
Botrytis were placed , after 1
day isolates were applied and
plants incubated at 20 ℃ for
10-14 days with 12 h
light/dark photoperiod
Fungus alone
Fungus alone
Fungus alone
25.1
25.1
284.2.8
284.2.8
Water
Water
100 ul of OD 0.5
PCT/62/885,386, 2020
Test for active molecule
Phase
separation
Control
DMSO 25.1 25.1 25.1
284.2.4 284.2.8
PCT/62/885,386, 2020
Bacillus subtilis
Pantoea agglomerans
Our Isolate
PCT/62/885,386, 2020
Other systems (under work)
Not_UVed water
20 % loss
UV water
80% loss
Host-microbiome interaction and the role of miRNA in cross and intra
kingdom communication under different health conditions.
PCT/IB2020/057943, 2020
Other systems (under work)
An innovative urban green roof and hydroponic system (iGrow) for urban
built environments in water scarce communities
Thank you
Dr. Ashraf Al Ashhab
052-6764006