A new species of the genus Klinzigedia Căpuşe, 1971 (Lepidoptera: Coleophoridae) from Iran

Abstract

A new species Klinzigedia sultankutensis Anikin, sp. n. is described from Iran, Mazandaran Province. The species resembles most closely Klinzigedia phlomidella (Christoph, 1862) but has well differences in its external appearance and genital morphology. Larva of the new species feeds on Phlomis herba-venti L. Adult male and female, their genitalia as well as larval case are illustrated and data on the known distribution are given. A male of the new species has elongated and pointed to the top sacculus with small rectangular tooth near apex, phallotheca almost twice shorter as in K. phlomidella. The antrum of a female of K. sultankutensis sp. n. is slightly expanded in the median part and is more narrowed to the caudal part of the ductus than in K. phlomidella. The number of bristled spinelets on each plate on the abdominal tergite I of K. sultankutensis sp. n. is 46–49 in male and 57–63 in female.

Full text

Кавказский энтомологический бюллетень 16(2): 219–223 © Caucasian Entomological Bulletin 2020
DOI: 10.23885/181433262020162-219223
Research Article / Научная статья
A new species of the genus Klinzigedia Căpuşe, 1971
(Lepidoptera: Coleophoridae) from Iran
© V.V. Anikin1, K.A.Kolesnichenko2
1Chernyshevsky Saratov State University, Astrakhanskay str., 83, Saratov 410026 Russia. E-mail: AnikinVasiliiV@mail.ru
2Lomonosov Moscow State University, Leninskie Gory, 1, Moscow 119991 Russia. E-mail: kkolesnichenko@gmail.com
Abstract. A new species Klinzigedia sultankutensis Anikin,sp.n. is described from Iran, Mazandaran Province. e species
resembles most closely Klinzigedia phlomidella (Christoph, 1862) but has well differences in its external appearance and genital
morphology. Larva of the new species feeds on Phlomis herba-ventiL. Adult male and female, their genitalia as well as larval
case are illustrated and data on the known distribution are given. Amale of the new species has elongated and pointed to the
top sacculus with small rectangular tooth near apex, phallotheca almost twice shorter as in K.phlomidella. e antrum of a
female of K.sultankutensissp.n. is slightly expanded in the median part and is more narrowed to the caudal part of the ductus
than in K.phlomidella. e number of bristled spinelets on each plate on the abdominal tergiteI of K.sultankutensissp.n. is
46–49in male and 57–63 in female.
Key words: Lepidoptera, Coleophoridae, Klinzigedia, new species, Iran.
Новый вид рода Klinzigedia Căpuşe, 1971 (Lepidoptera: Coleophoridae) из Ирана
© В.В. Аникин1, К.А. Колесниченко2
1Саратовский национальный исследовательский государственный университет имени Н.Г. Чернышевскогo, ул. Астраханская, 83,
Саратов 410026 Россия. E-mail: AnikinVasiliiV@mail.ru
2Московский государственный университет имени М.В. Ломоносова, Ленинские Горы, 1, Москва 119991 Россия. E-mail:
kkolesnichenko@gmail.com
Резюме. Новый вид Klinzigedia sultankutensis Anikin, sp.n. описан из Ирана, провинция Мазандаран. Вид наиболее
близок к Klinzigedia phlomidella (Christoph, 1862), но имеет хорошие отличия во внешнем виде и в морфологии
гениталий. Гусеница нового вида питается на Phlomis herba-ventiL. Приведены иллюстрации имаго обоих полов, их
генитальных структур, чехлика гусеницы. Самец нового вида имеет удлиненный и заостренный к верхушке саккулус
с небольшим прямоугольным зубцом у вершины, фаллотека почти вдвое короче, чем у K. phlomidella. У самки
K.sultankutensissp.n. антрум немного расширен в срединной части и более сужен к каудальной части дуктуса, чем
у K.phlomidella. Число шиповидных хет на каждой пластинке брюшного тергитаI у самца K. sultankutensis sp. n.
составляет46–49, а у самки57–63.
Ключевые слова: Lepidoptera, Coleophoridae, Klinzigedia, новый вид, Иран.
ZooBank Article LSID: urn:lsid:zoobank .org:pub:8F8B7120-2328-4BA0-B0D3-CD057717E70D
Introduction
Alot of species belonging to several genera have been
known and described from Iran during the last century
by specialists on microlepidoptera [Toll, 1952, 1959a,b,c;
Amsel, 1977; Baldizzone, 1994a,b]. More than 170species
were known from Iran to the end of the 20th century
[Baldizzone et al., 2006].
Species of the genus Klinzigedia Căpuşe, 1971 are
usual for various open landscapes of steppe and forest-
steppe in southern regions of the Palaearctic in lowlands
and mountains up to 3000m. is small Palaearctic genus
includes seven species (K. anelpista (Baldizzone, 1994),
K. implicitella (Fuchs, 1903), K. inusitatella (Caradja,
1920), K. onopordiella (Zeller, 1849), K. phlomidella
(Christoph, 1862), K. phlomidis (Stainton, 1867),
K.wockeella (Zeller, 1849)). Known larvae feed exclusively
on plants of the genus Phlomis (family Lamiaceae) having
native distribution from the Mediterranean region, across
Central Asia to Inner Mongolia in China. Stenoligophagy
of those casebearers determines the range of the genus
Klinzigedia corresponding to distribution of the genus
Phlomis [Anikin, 2007, 2010].
Below we descibe a new species from Iran belonging
to the genus Klinzigedia from the tribe Klinzigediini due
very characteristic habitus, ochreous groundcolor with
linel wing pattern, shape of labial palps, equipment of
abdominal tergites and the structure of genitalia.
Material and methods
e material was collected by the second author during
his expedition in late April-early May 2017 in the Elborz
Mountain area and the Turkmen-Khorasan Mountains
(Figs1,2). e feeding caterpillars of the new species were
found on leaves of Phlomis herba-venti (Fig.3). e larvae
mined the lower side of the host plant leaves (Fig.4) like
the most other caterpillars of this family. Collected larvae
in cases (Fig.5), after feeding for a few more days, pupated
and imagos emerged in 2–3 weeks in a laboratory. All
photos in nature were taken by K.A.Kolesnichenko.
Five preparations were processed following standard
techniques for morphological studies of the genitalia
[Robinson, 1976]. e photos of adult specimens and
larval case were taken with an Olympus Tough TG-5.
e genitalia were photographed from genitalia slides

in ventral view with Mikmed-6 Microscope with camera
Levenhuk C1400 NG. All photos are taken by the first
author.
e material is depositerd in:
ZISP – Zoological Institute of the Russian Academy of
Sciences (St Petersburg, Russia);
SarSU – Saratov State University (Saratov, Russia).
Klinzigedia sultankutensis Anikin, sp. n.
(Figs 4–12)
Material. Holotype, ♂ (ZISP): “IRAN, Mazandaran Prov.,
Smacroslopes of Alburs Mts., ESultan-KutMt., 80km SE Sarit, 5km NE
Folladmahalehv., N36°06ʹ14ʺ E53°43ʹ16ʺ, 20–21.05.2017, K.Kolesnichenko
leg., exl. 21.05.2017 on Phlomis herba-ventiL.”. Paratypes: 5♂ (1♂ in ZISP,
4♂ in SarSU), 2♀ (1♀ in ZISP, 1♀ in SarSU), same locality and date as
holotype, ex l. 9–31.05.2017, on Phlomis herba-ventiL.
Diagnosis. e new species is the closest to
Klinzigedia phlomidella described from southern part
of Russia (“bei Sarepta”) by Christoph [1862]. In contrast
to K. phlomidella (Christoph, 1862) which has small
pointed top of the sacculus without tooth in male genitalia,
Klinzigedia sultankutensissp.n. has elongated and pointed
to the top sacculus with small rectangular tooth near apex.
e new species has phallotheca almost twice shorter
as in K. phlomidella. e right rod of the phallotheca in
K. sultankutensis sp. n. on dorsal margin is with two
rectangular pointed teeth, the smaller one is located near
the top and the bigger one is on 1/3 length from the top,
while K. phlomidella has only one large tooth on the top
of the right rod. Klinzigedia sultankutensis sp. n. has in
vesica one long cornutus, the same as the tegument length,
slightly curved in the median part and raised in front,
but K. phlomidella has the cornutus smoothly curved
along the entire length and shorter than tegumen on1/3.
In a female of K. phlomidella the basal part of sterigma
is elongated rectangular, shorter and wider that one in
K. sultankutensis sp. n. e antrum of K. phlomidella is
more expanded in the median part and is not narrowed
to the caudal part of the ductus. e number of bristled
spinelets on each plate on the abdominal tergite I in
K. sultankutensis sp.n. is twice more numerous than in
K.phlomidella.
Description. Imago (Figs 6,7). Wingspan of male 26–27.5mm,
female – 27–28.3mm. Head, thorax and postocular scales light
ochreous. Proboscis very long, almost reachs the end of abdomen.
Labial palpus ochreous. Antenna yellowish-ochreous, annulated
with dark yellow rings, scapus concolorous with head. Basal antennal
segment 4times longer than wide, forms short brush. Antennae
on half of its length covered with loose adjoining scales, segments
of flagellum length 0.5times longer than wide, slightly ringed in
upper part. Forewing ochreous, suffused with light yellowish-ochre
scales in basal part, veinsRandM clearly visible and covered with
dark ochre scales. Costal fringe long, basally concolorous with
light wing apex. Hindwing and fringe pale dark brown. Abdomen
pale yellowish-ochreous, tergal patches 2times longer than wide.
Larva and case. Feeds and pupates in case (Fig.5), mining the
lower surface of leaf on Phlomis herba-ventiL. (Lamiaceae). e
larva makes case bounding the small pieces of the host plant leaves
by silk. Case 18–22 mm long and 5–7 mm wide, grayish-yellow
color, with brown tinge. e larva develops from April to May.
Male genitalia (Fig. 8). Gnathos almost oval, arms twice
long. Tegumen narrow, extending basally, pedunculi very short.
Cucullus finger-shaped long, half extended beyond sacculus.
Valvula as broad as cucullus, ventral margin rectangularly shaped,
surface covered with rare short bristles. Transtilla broad, mace-
shaped, sharply tapered towards apex. Sacculus well sclerotized;
elongated to the top and pointed, with small rectangular tooth
Fig. 1. e type locality of Klinzigedia sultankutensis sp. n.
Рис. 1. Типовое местонахождение Klinzigedia sultankutensis sp. n.
Figs 2–3. Biotope and host plant of Klinzigedia sultankutensis sp. n.
2 – lower part of the gorge in the Foulad Mohaleh village vicinity, Mazandaran Province, Iran; 3 – Phlomisherba-ventiL. (Lamiaceae).
Рис. 2–3. Биотоп и кормовое растение Klinzigedia sultankutensis sp. n.
2 – нижняя часть ущелья в окрестностях села Ф улад Мохале, провинция Мазандаран, Иран; 3 – Phlomisherba-ventiL. (Lamiaceae).
220 V.V. Anikin, K.A.Kolesnichenko

Figs 4–7. Cases and imagos of Klinzigedia sultankutensis sp. n.
4–5 – cases: 4 – with larva mining on the leaf underside of Phlomis herba-venti, 5 – case of larvae; 6–7 – imagos: 6 – male, holotype, 7 – female,
paratype.
Рис. 4–7. Чехлики гусеницы и имаго Klinzigedia sultankutensis sp. n.
4–5 – чехлики: 4 – мина гусеницы на обратной стороне листа Phlomis herba-venti, 5 – чехлик гусеницы; 6–7 – имаго: 6 – самец, голотип, 7 –
самка, паратип.
near apex, ventral margin rounded, ending in sharp angle; dorsal
margin lightly convex. Phallotheca dorsally expanded, with two
dark sclerotized rods, left rod on dorsal margin with rectangular
sharped tooth on 1/4 length from the top, right rod on dorsal
margin with two rectangular sharped teeth, smaller one located
near the top and bigger one on 1/3length from the top. In vesica
one longer cornutus same as tegument length, slightly curved in
the middle and raised in front.
Abdominal tergites of male (Fig.9). Tergal disk about 2times
longer than wide. Abdominal tergiteI bristled by 46–49spinelets
on each plate; patches shorter on the following tergites and have
65–67spinelets on each.
Female genitalia (Figs 10,11). Ovipositor elongate, telescopic.
Papillae anales very narrow, medially as wide as 1/5of the length.
Anterior apophyses 0.3 times shorter than sterigma length,
posterior apophyses 3.3 times longer than anterior apophyses.
Sterigma sclerotized well laterally at base of apophyses and has
elongate rectangular shape, 3.5times as wide as long, distal part
triangular shape, with edge slightly concave relative to ostium
bursae, caudal margin strong rounded, with a row of 7–8 not
long bristles on each side of ostium. Ostium broadly U-shaped,
wide and deep almost to middle of plate. Antrum goblet shaped
slightly expanded in the middle, very short and well sclerotized.
Ductus bursae very long, thin and transparent, caudal part with
two short rods (without central) covered with small dark spinules,
middle part bag-shaped, covered with little spines on the right
inner surface. Corpus bursae elongate, signum relatively large, base
narrow, spine slender, almost straight.
Abdominal tergites of female (Fig. 12). Tergal disk about
1.5 times longer than wide. Abdominal tergite I bristled
by 57–63spinelets on each plate; patches a bit narrower on the
following tergites and have 73–76spinelets on each.
e type locality description. e type locality
of the new species is montane stepped landscapes with
shrubs along the southern slopes (Fig. 2). e gorge is
located parallel to the Elborz Range, 5km north of Foulad
Mohalleh village in Semnan Province, Iran. e altitude
is 2015m a.s.l., the mountain peaks exceed 2300 m. e
stabilized clayed coarse-rubble scree with rock outcrops
is covered with Juniperus polycarpos sparse forest. Acer
monspessulanum persicum can be found at the foot of
the slope. e shrub layer is represented by two species of
Cerasus, Spiraeasp., Berberissp., and Rosasp. e lower
tier comprises polster xerophytic shrubs and dwarf shrubs:
Artemisia spp., Acantholimon spp., Onobrychis cornuta,
Astragalus spp. (including Tragacantha). In May the soil
is covered with sparse grass sod of Gramineae containing
some annual Compositae (Asteriaceae), Cruciferous
(Brassicaceae), Geranium sp., Ferula sp., Tulipa lehmanii,
Cousinia thomsonii and Phlomis herba-venti.
Distribution. Iran, Mazandaran Province.
Etymology. e new species is named after its type
locality in Iran near mountain Sultan-Kut, where it was
collected.
Discussion
Klinzigedia sultankutensis sp. n. has all diagnostic
characters for species of the genus Klinzigedia which were
allocated to the separate tribe Klinzigediini on the basis of
A new species of the genus Klinzigedia Căpuşe, 1971 (Lepidoptera: Coleophoridae) from Iran 221

Figs 8–12. Klinzigedia sultankutensis sp. n., details of structure.
8 – male genitalia (holotype); 9 – abdominal tergites of male; 10 – female genitalia (paratype); 11 – antrum; 12 – abdominal tergites of female.
Рис. 8–12. Klinzigedia sultankutensis sp. n., генитальные структуры.
8 – гениталии самца (голотип); 9 – абдоминальные тергиты самца; 10 – гениталии самки (паратип); 11 – антрум; 12 – абдоминальные тергиты
самки.
biological and morphological characteristics [Falkovitsh,
2003]. e monophyly of the tribe was supported using
the genetic marker COI mDNA [Anikin et al., 2016a, b].
e larvae of species of this genus have one main
peculiarity, known caterpillars of four species (K.anelpista,
K.inusitatella, K.phlomidella, K.phlomidis) feed on plants
of the genus Phlomis and one species (K. wockeella) on
plants of the close genera– Stachys, Betonica, Ballota, and
belong to the steno-oligophagous ecological group. Rarely
mature caterpillars of K. onopordiella can finish feeding
on Asteraceae [Falkovitsh, 1996]. According to our studies
the larva of K. sultankutensissp. n. has only one host plant
Phlomis herba-venti which confirm the stenoligophagy of
Klinzigedia species.
Acknowledgements
We extend our sincere thanks to anonymous reviewers
for their careful corrections and helpful comments to
improve the quality of this manuscript. anks to curators
of various collections who supported our study of museum
funds: DrSergey Sinev (StPetersburg, Russia), DrWolfram
Mey (Berlin, Germany), Dr Robert Trusch (Karlsruhe,
Germany). We say our special thanks to Dmitry Lyskov
(Faculty of Biology, Lomonosov Moscow State University,
Moscow, Russia) for determination of host plant and
to Dr Vadim Zolotukhin (Ulyanovsk State Pedagogical
University, Ulyanovsk, Russia) for commenting the
manuscript.
222 V.V. Anikin, K.A.Kolesnichenko

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A new species of the genus Klinzigedia Căpuşe, 1971 (Lepidoptera: Coleophoridae) from Iran 223
Received / Поступила: 15.04.2020
Accepted / Принята: 20.07.2020
Published online / Опубликована онлайн: 6.10.2020