Article

Optimization of Extraction of Polyphenolic Compounds from Medicinal Lungwort (Pulmonaria officinalis L.)

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Abstract

Medicinal plants are potential natural sources of biologically active substances that have geroprotective properties, slowing down the aging process. Vegetable raw materials are used as antioxidants in the food industry. Pulmonaria officinalis L. has a high content of biologically active substances. The purpose of this work is to determine the operating parameters for extracting the maximum amount of biologically active substances from Pulmonaria officinalis L. by extracting ethanol and finding sources of natural antioxidants.The antioxidant activity of several ethanol extracts of Pulmonaria officinalis L. – 30-70% with a step of 10 ethanol obtained from the dried root culture of the medicinal plant of biologically active substances was determined. The total content of polyphenols, flavonoids and proanthocyanidins was estimated. According to the data obtained, the relationship between the values determined for the biologically active substances presented and the values of antioxidant activity was revealed. Extracts of Pulmonaria officinalis L. showed a high yield of polyphenolic compounds-889.39±4.29 mcg of Gallic acid/ml, flavonoids-728.90±6.98 mcg of rutin/ml, proanthocyanidins-211.65±5.31 mcg of catechin/ml at an ethyl alcohol concentration of 60% and an extraction temperature of 50°C in 4 hours. The antioxidant activity using 2 methods: A spectrophotometric method DPPH (2,2-diphenyl-1-picrylhydrazyl) and the FRAP method (iron reducing/antioxidant power) was investigated. During DPPH spectrophotometric analysis, the antioxidant activity is equal to 86.96% for polyphenols, 75.47% for flavonoids, and 51.25% for proanthocyanidins. FRAP analysis showed that extracts with operating parameters had a pronounced antioxidant activity (tэ= 4 ч; Tэ= 50°C; Сэ= 60%)и (tэ= 5 ч; Tэ= 60°C; Сэ, = 70%).

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... The root culture of P. officinalis was used to prepare extracts in different concentrations of ethyl alcohol to optimally obtain the biologically active substances [21]. The total content of polyphenols in the P. officinalis L. extract was determined using the Folin-Ciocalteu method. ...
... The determination of proanthocyanidins was carried out using vanillin analysis in glacial acetic acid. The P. officinalis extract showed a high yield of polyphenolic compounds, i.e., 889.39 ± 4.29 µg gallic acid/mL, flavonoids 728.90 ± 6.98 µg rutin/mL, and proanthocyanidins 211.65 ± 5.31 µg catechin/mL, respectively [21]. The content of these of types of phytochemicals was higher in the root culture extract than in the dried part of P. officinalis obtained in the earlier study [21]. ...
... The P. officinalis extract showed a high yield of polyphenolic compounds, i.e., 889.39 ± 4.29 µg gallic acid/mL, flavonoids 728.90 ± 6.98 µg rutin/mL, and proanthocyanidins 211.65 ± 5.31 µg catechin/mL, respectively [21]. The content of these of types of phytochemicals was higher in the root culture extract than in the dried part of P. officinalis obtained in the earlier study [21]. ...
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