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Influence of growth media and regulators on vegetative propagation of rosemary ( Rosmarinus officinalis L.)

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Abstract

Rosemary (Rosmarinus officinalis) is a woody, perennial herb with fragrant evergreen needle-like leaves and a lovely fragrance. Leaves are finely chopped and used to flavour dishes. Rosemary propagation is done through either seed or stem cutting. Seeds of rosemary are rarely used in propagation as they are slow to germinate, taking 3–4 weeks before emergence with a poor germination rate of 10–20%. Propagation of plants from cuttings,enables a large percentage of the cuttings to produce roots quickly, and using a rooting hormone increases the success rate of creating new plants. This study was initiated to determine the best growth media and growth hormone for use in rosemary propagation. The trial was laid out in a two factor randomized complete block design (RCBD) with four replications. Four growing media (1) vermiculite, (2) top soil/manure/sand mixture at ratio 10:3:1 (top soil mixture), (3) top soil only and (4) sand, in combination with four growth hormones (i) Baby Bio (Roota²), (ii) Roothom H, (iii) Anatone 3 and (iv) control were evaluated. A significant difference was observed with the use of growth hormones, with the highest mean root number observed in the sand media in combination with Roothom H (48.73) while the control (no hormone) gave the lowest in vermiculite (9.34). Roothom H gave the best performance in mean root numbers across the four media treatments and therefore is recommended to stimulate root growth in cuttings.

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... Rosemary is an evergreen, perennial, and tufted shrub, with erect or procumbent growth and a maximum height, usually, up to 1.8 m [1]. It is propagated easily by seeds, cuttings, layering, or root division [2][3][4]. Although it derives from the Mediterranean basin, it is hardly enough that it can be cultivated in a vast spectrum of environmental conditions. ...
... Seeds of rosemary are rarely used in propagation as they are slow to germinate, taking 3-4 weeks before emergence, with a poor germination rate of 10-20% [3]. Therefore, vegetative propagation is the most common method for propagation. ...
... A mixture of peat and perlite (1:1, v/v) was referred to, by Varban et al. [24], as the most effective substrate for the rooting of rosemary stem cuttings. Kiuru et al. [3] also tested four different substrates (vermiculite, topsoil-manure-sand mixture at a ratio of 10:3:1, topsoil, and sand), on the propagation of rosemary by cuttings, and concluded that the sand was the most efficient one. Furthermore, Mendoza-Hernandez et al. [25] tested the replacement of peat, in the rooting substrate, with compost and various vermicomposts of horticultural waste. ...
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A comprehensive collection of 1254 tomato accessions, corresponding to European traditional and modern varieties, early domesticated varieties, and wild relatives, was analyzed by genotyping by sequencing. A continuous genetic gradient between the traditional and modern varieties was observed. European traditional tomatoes displayed very low genetic diversity, with only 298 polymorphic loci (95% threshold) out of 64 943 total variants. European traditional tomatoes could be classified into several genetic groups. Two main clusters consisting of Spanish and Italian accessions showed higher genetic diversity than the remaining varieties, suggesting that these regions might be independent secondary centers of diversity with a different history. Other varieties seem to be the result of a more recent complex pattern of migrations and hybridizations among the European regions. Several polymorphic loci were associated in a genome-wide association study with fruit morphological traits in the European traditional collection. The corresponding alleles were found to contribute to the distinctive phenotypic characteristic of the genetic varietal groups. The few highly polymorphic loci associated with morphological traits in an otherwise a low-diversity population suggests a history of balancing selection, in which tomato farmers likely maintained the morphological variation by inadvertently applying a high selective pressure within different varietal types.
... Rosemary is an evergreen, perennial, and tufted shrub, with erect or procumbent growth and a maximum height, usually, up to 1.8 m [1]. It is propagated easily by seeds, cuttings, layering, or root division [2][3][4]. Although it derives from the Mediterranean basin, it is hardly enough that it can be cultivated in a vast spectrum of environmental conditions. ...
... Seeds of rosemary are rarely used in propagation as they are slow to germinate, taking 3-4 weeks before emergence, with a poor germination rate of 10-20% [3]. Therefore, vegetative propagation is the most common method for propagation. ...
... A mixture of peat and perlite (1:1, v/v) was referred to, by Varban et al. [24], as the most effective substrate for the rooting of rosemary stem cuttings. Kiuru et al. [3] also tested four different substrates (vermiculite, topsoil-manure-sand mixture at a ratio of 10:3:1, topsoil, and sand), on the propagation of rosemary by cuttings, and concluded that the sand was the most efficient one. Furthermore, Mendoza-Hernandez et al. [25] tested the replacement of peat, in the rooting substrate, with compost and various vermicomposts of horticultural waste. ...
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... Rosemary oil demonstrated the lowest inhibitory concentration against Escherichia coli. The antibacterial properties of rosemary oil have been demonstrated in several investigations against Salmonella choleraesuis, Clostridium perfringens, Bacillus cereus, E. coli, and Staphylococcus aureus (16,17). ...
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Rosemary is one of the aromatic herb plants that is known to challenges in its seed production because the propagation of rosemary still relies on seed germination with low germination rates. To overcome this. This research aims to determine the growth response of rosemary stem cuttings to the application of Plant Growth Regulator (PGR) in the form of coconut water, variations in growing media, and their interaction. The study was conducted using a Completely Randomized Design with the variation of growing media and different concentrations of coconut water PGR solution. The experiment was conducted in a greenhouse, with a total of 15 treatment combinations repeated 3 times. The observed parameters included root length, number of roots, and number of shoots, with observations made every two weeks. The results showed that the combination of coconut water concentration and variation in growing media significantly interacted with the growth parameters, namely root length and number of roots. Additionally, individual factors such as the mixed soil and rice husk charcoal growing medium and 75% coconut water concentration separately had a significant effect on the number of sprouts at 42 and 56 days after planting.
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The aim of this research was to determine the effect of honey-cinnamon paste as a growth regulator on the layering of calamansi (Citrus microcarpa) grafts. This research used a simple randomized block design consisting of three treatments with four replicates. Four trees were selected for each replicate as parent plants. The growth regulator concentration treatments used were control (no growth regulator added); 146.67 g Rootone F/100 ml water (P1); and 36.67 g honey-cinnamon paste/ 100 ml of honey (P2). Both growth regulators showed similar effects and better results than the control treatment on the layering of the calamansi graft i.e. root number and length. Honey-cinnamon paste acts as an alternative to synthetic phytohormone because it contains enzymes and monosaccharide compounds (glucose and fructose) which can stimulate roots in calamansi layering. In addition, the antibacterial and antifungal in honey-cinnamon paste can also provide better environment for graft layering. ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian pasta kayu manis-madu terhadap pembentukan akar pada cangkok kalamansi. Penelitian menggunakan Rancangan Acak Kelompok (RAK) sederhana dengan 3 perlakuan dan 4 ulangan. Dari masing-masing ulangan dipilih 4 pohon sebagaitanaman induk. Perlakuan konsentrasi ZPT yang digunakan yaitu kontrol (tanpa ZPT), Rootone F (P1) sebanyak 146,67 g Rootone F per 100 ml air dan pasta kayu manis-madu (P2) dengan komposisi 36,67 g bubuk kayu manis per 100 ml madu. Hasil penelitian menunjukkan pemberian pasta kayu manis-madu memiliki pengaruh yang sama efektifnya dengan ZPT sintetis Rootone F dan keduanya memberikan hasil yang lebih baik dari kontrol terkait variabel jumlah dan panjang akar pada cangkok kalamansi. Pasta kayu manis-madu berperan sebagai fitohormon alternatif karena mengandung enzim dan senyawa monosakarida (glukosa dan fruktosa) yang dapat menstimulasi perakaran pada cangkok kalamansi. Selain itu, kandungan antibakteri dan antijamur pada pasta kayu manis-madu dapat mendukung kondisi perakaran cangkok yang lebih baik. Kata Kunci: Cangkok, Fitohormon Alternatif, Kalamansi, dan Pasta Kayu Manis-Madu.
... Meanwhile, vegetative propagation has advantages: the similar genetic characteristics as parent tree, seed can be produce anytime independent from fruiting season, easy seed provision and in large quantities, produce yield faster than seed propagated-plants. However, plant root was usually shallow and widespread, although it will develop following plant age [5]. Furthermore, vegetative propagation is also useful for developing clone bank (genetic conservation) [6]. ...
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Cinnamon can be propagated generatively and vegetatively. Vegetative propagation will generate the similar characteristics as its parent tree, hence facilitating the providing of high quality seedling. The research objective was to obtain the suitable vegetative propagation technology for cinnamon by cutting. The research was conducted at Cimanggu Research Installation, ISMCRI, Bogor from January to December 2018. The experiment was designed in randomized block design, 2 factors and 3 replications. The first factor was cutting materials : shoot cutting from 1) the 1 st -2 nd node 2) the 3 rd -4 th node and 3) the 5 th -6 th node. The second factor was planting medium 1) manure: husk charcoal: soil (2: 1: 1), 2) manure: cocopeat: soil (2: 1: 1), and 3) water. The shoot cutting from the 5 th -6 th node indicated better plant growth than from the 1 st -2 nd node, although not significantly different from the 3 rd -4 th node. Cuttings grown in water medium indicated no new shoot emergence until the last observation. However, cuttings planted in charcoal or cocopeat media, grew well and showed no significant differences in all parameters. Cinnamon plant can be propagated vegetatively by cutting using shoot cuttings from 5 th -6 th node or the 3 rd -4 th node grown in media contained husk charcoal or cocopeat.
... Propagation of medicinal and aromatic plants by stem cuttings is the most vital method to reproduce plants (Anderson &Woods, 1999 andWaman et al., 2019). It offers production of true-to-type plants in a short time and availability of superior individuals for large scale commercial plantation with quick productive gains (Kiuru et al., 2015). ...
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Rosemary (Rosmarinus officinalis L.) is an important medicinal and aromatic herb, used by various pharmaceutical, food and cosmetics industries. For most species, seed-based multiplication is effective and convenient, but for rosemary, seed germination is a problem due to the mucilaginous seed coat, and sometimes the germination is no more than 10–20% resulting in non-uniform crop establishment. This investigation was carried out to improve seed germination by using low-cost organic seed priming techniques. Eleven pre-sowing seed treatments were tested, based on locally available resources, including: soaking seeds in water (cold and hot), bovine urine (5%, 10%, 15% and 20% concentration), bovine dung slurry, buttermilk, compost tea and Agnihotra ash, and comparing with an untreated control. All the pre-sowing seed treatments increased the percentage of seed germination in rosemary compared with the control, except buttermilk, which had an adverse effect. Among the different treatments, seed germination was significantly increased to 46.7% for seeds treated with bovine dung slurry and to 42.0% for seeds treated with bovine urine5.0%, compared with those in the control where the germination was 9.7%. The bovine dung slurry treatment also recorded uniform and early germination (9 days for initiation and completion at 43 days) resulting in a significantly higher rate of germination (1.4) than the control. The number of leaves, the root length and the seedling vigour were also recorded to be the highest for seeds treated with bovine dung slurry, followed by bovine urine5.0%.
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