“One Health” has been defi ned as “the collaborative eff ort of multiple disciplines — working locally, nationally, and globally — to attain optimal health for people, animals and the environment”. One Health is a new initiative, but with the concept that extends back to ancient times. One Health is an interdisciplinary concept for complex health challenges from a holistic integrated perspective, ... [Show full abstract] more then a divided perspective based on different disciplines. Th ere is no isolation, wild animals, domestic animals, pathogens and diseases do not know of the political borders. The aim of the One Health initiative is to form unifi ed solutions applicable for the improvement of health of humans, animals and the environment. A workshop was organized for the representatives of all structures and levels of medical and veterinary services of Serbia. During four tasks, among joint working groups, the most important structure of One Health was proposed, introducing possible concept in Serbia.