In plants, light quality plays significant roles in photomorphogenesis and photosynthesis. Efficient in vitro plant propagation techniques involve tailoring of various environmental cues and culture media according to the plant species. Plant tissue culture consists of several applications in scientific research, agriculture, biotechnology, and commercial industrial purposes. Utilization of light ... [Show full abstract] to enhance the quality of the in vitro raised plants have been evidenced by numerous researchers in plant tissue culture. The advent of light-emitting diode- (LED-) based artificial lighting systems in plant tissue culture for micropropagation has enhanced callus induction, shoot and root organogenesis, and acclimatization of in vitro propagated plants. Plants tend to perceive the light spectra present in the photosynthetically active region (PAR) ranging from 400 to 700 nm; this includes blue and red light wavelengths. Although the influence of spectral quality is being investigated in diverse plant species, particularly, its importance in in vitro propagated horticultural crops is gaining notable interest among researchers. In recent days, the application of LEDs provides better amenability according to the plant species of interest for efficient plant regeneration. Considering the growing necessity and emerging applications of LED supplemental lights for propagation of plants in in vitro, the present review summarizes the outcomes of various research studies dealing with LEDs in plant tissue culture. Moreover, the present endeavor has provided a comprehensive overview on the effects of LEDs in the morphogenesis of plants cultured in vitro.