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European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 43 www.idpublications.org
EFFECT OF INCLUSION OF PIGEON PEA FLOUR ON THE NUTRITIONAL,
FUNCTIONAL AND SENSORY CHARACTERISTICS OF SNACKS FROM
BREADFRUIT FLOUR
Adepeju, A. B. 1, Abiodun, O. A.2, Ikuomola, D. S.1 & Esan, Y. O.1
Department of Food Science and Technology
1. Joseph Ayo Babalola University, Ikeji Arakeji, Osun State, NIGERIA
2. University of Ilorin, Kwara State, NIGERIA
ABSTRACT
The effect of pigeon pea flour (PF) on the nutritional, functional and sensory quality of
snacks from breadfruit flour (BF) were investigated. Products with PF were more yellow in
color, had higher Protein, Ash, Crude fibre, bulk density and water absorption capacity.
Snacks with 95% BF: 5% PF had a suitable crisp to hard texture. All the snack products were
liked moderately to very much in overall acceptability.
Keywords: Nutritional, Functional, Pigeon pea, Bulk density, Water Absorption capacity.
INTRODUCTION
Snacks can be considered as tasty, savory or sweet foods eaten at non-meal occasions. They
are small meals eaten between meals. Snacks provide convenience and manageable portions,
and they fulfilled short-term hunger (Eneche, 1999). Snacks tend to be high in calories and
fat and low in protein and other nutrients (Rahma and Mustafa, 1998). Healthy snacks help to
bridge the gap between meals. Breadfruit-pigeon pea snack is a snack made from breadfruit
flour, pigeon pea flour, ground yeast and other ingredient such as pepper, salt/sugar, mono
sodium glutamate and water.
Breadfruit (Artocarpus altilis) is one of the local staples in the developing countries, although
relatively cheap and nutritious but neglected (Akanbi et. al., 2009). It belongs to the Mulberry
family Moraceae. It is a tropical fruit and the tree produces fruit twice a year, from March to
June and July to September with some fruiting throughout the year (Omobuwajo, 2007). It is
one of the principal sources of energy, protein, vitamin and minerals for millions of the
poorest people (Tuivavakgi and Samuelu, 2007). Its nutritive value especially carbohydrate,
protein, fat and mineral contents is comparable or even superior to some cereal food grains
(Adebowale et. al., 2008). Breadfruit is of high protein quality unlike most of cereals
especially maize which is generally recognized as being inherently poor in protein quality
(Omobuwajo, 2007; Uvere et. al.,2002). Pigeon pea (Cajanus cajan (L) Millsp.) is a
nutritionally important grain legume of the tropical and subtropical regions of the world
(Singh et. al.,1991). It is ranked 5th in importance among edible legumes of the world (Duhan
et. al.,1999). Sherry, (2007) found that the protein content of pigeon pea seed samples
ranged between 18.5% and 26.3% with a mean of 21.5%. Protein quality is of prime
importance in pigeon pea products used for human food (Salunkhe et. al.,1996). Pigeon pea
protein is a rich sources of lysine but it usually deficient in the sulfur- containing amino
acids, methionine and cystine (Salunkhe et. al., 1996 ). According to Singh et, al.,(1991)
snacks of blended proteins could increase nutritional value and create better texture or other
functional properties. Pigeon pea flour was added to breadfruit flour to increase the protein
European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 44 www.idpublications.org
content and improve texture of the snack. The objectives of this research work is to
investigate the effect of addition of pigeon pea flour to breadfruit flour on the nutritional,
functional and sensory characteristics of the flavored snacks produced.
MATERIALS AND METHODS
Materials
Breadfruit (Artocarpus altilis) were obtained from Ifewara Osun State ,Nigeria. Pigeon pea
(Cajanus cajan (L.) Millsp.) were obtained from a central market in Ilesa, Osun State.
Processing of breadfruit into flour
Breadfruit was processed into flour as shown in Figure 1. The breadfruit was thoroughly
washed to remove dirt and unwanted materials. It was then peeled and washed with clean
water. The breadfruit was sliced, blanched for about 5 min. and then dried in the oven at 105
0C for 1-3 h, after which it was milled into flour. The flour was screened through a 0.25 mm
British Standard Sieve (Model BS 410) (Giami , 1993).
Processing of Pigeon pea into flour
Pigeon pea (Cajanus cajan (L.) Millsp.) seeds were processed into flour using the procedure
by Singh et.al., (1991). An oil pretreatment using 1% corn oil was used to dehull the pigeon
pea seeds . The dried pigeon pea seeds were ground in a Victoria grain mill (Low Hooper,
Medellin, Colombia), screened through a 0.25 British Standard Sieve (Model BS 410).
Figure 2 showed the flow chart for the processing of pigeon pea flour.
Blend Formation
Four blends were prepared by mixing Pigeon pea flour with Breadfruit flour in the
percentage proportions of 0:100; 5:95; 10:90 and 15:85 respectively, using machine food
processor (Kenwood KM 201, England). Baking powder, pepper, salt/ sugar, were added to
each blend to obtain a desired consistency. The dough was thoroughly mixed and cut into
shape manually and baked at 220 0C for 15 min, allowed to cool, packed and stored for other
determinations.
Methods of analysis
Proximate analysis was carried out on the snacks using the methods of the Association of
Official Analytical Chemists (AOAC, 2000). Water absorption capacity of the sample was
determined by the method of AACC (1995). The bulk density was determined as described
by Okezie and Bello (1988) and expressed in g/ml.
Swelling capacity was determined by the method described by Takashi and Sieb (1988).
Sensory evaluation was conducted in 2 stages. In the 1st stage, trained panelist rated the
texture of the unflavored snacks to determine the most suitable textured products. In the 2nd
stage, attributes of color, odor, flavor, texture and overall acceptability of the flavored snacks
products were evaluated by hedonic testing using consumer panelists. The texture of
unflavored snack-containing 5% PF, 10% PF and 15% PF – was rated on a 5- point scale: 5 =
very hard, 4 = hard, 3 = crisp, 2 = soft, 1 = very soft. Sensory evaluation was performed
using 10 panelists. Panelists were instructed to consider only texture and ignore the other
European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 45 www.idpublications.org
attributes. Panelists were instructed to place the snacks between molars and bite down slowly
and evenly until a fracture or break occurs. The descriptor for texture were very soft = a low
perceived force required to compress the snack, soft = a moderate perceived force required to
compress the snack,
crisp = as the perceived force in which the snack fractures or crumbles into small pieces, hard
= a high perceived force required to compress the snack and very hard = an extreme
perceived force required to compress the snack. Twenty- five consumer panelists evaluated
the flavored snacks during 2 sessions for color, odor, flavor, texture and overall acceptability.
The products were evaluated on a 9 – point hedonic scale. 9 = like extremely, 8 = like very
much, 7 = like moderately, 6 = like slightly, 5 = neither like nor dislike 4 = dislike slightly, 3
= dislike moderately, 2 = dislike very much, 1 = dislike extremely. The scores were analyzed
by analysis of variance to determine the effects of adding pigeon pea flour to breadfruit flour.
Breadfruit
Cleaning
Peeling
Washing
Slicing
Blanching
Drying
Dry milling
Sieving
Screening
Breadfruit flour
Fig. 1: Flow chart for the production of breadfruit flour.
European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 46 www.idpublications.org
Pigeon pea seeds
Sorting/Cleaning
Pretreatment
Dehulling
Milling
Sieving
Sreening
Pigeon pea flour
Fig.2. Flow chart for the production of pigeon pea flour.
RESULTS AND DISCUSSION
Proximate composition
Table 1 show the effects of inclusion of pigeon pea flour (%) to Breadfruit flour on the
proximate composition of the snacks produced. As expected the addition of pigeon pea flour
increased the non carbohydrate components, particularly the crude protein contents of the
snacks and reduced the total carbohydrates. The protein content of both BF and PF is high,
although that of PF is higher.
The crude fibre (%) ranged between 2.05 and 2.66 and this showed a corresponding
increase with increase in the proportion of pigeon pea flour . Pigeon pea has relatively higher
crude fibre than breadfruit and this justifies the values of crude fibre obtained for the
different snack samples. This observation compares well with the findings of Esuoso and
Bamiro, ( 1995) as well as Amusa et. al., (2002). Crude fibre is known to aid the digestive
system of human (Ihekononye and Ngoddy, 1985), indicating that the snacks could attract
good acceptability by many people as well as health organizations.
The value obtained for the ash contents indicated that the value increased with increase in
pigeon pea substitution. The ash content of pigeon pea was higher than that of breadfruit and
could be responsible for the increase in ash obtained. It then follows that incorporation of
pigeon pea in the process of snack making could enhance the minerals intake of many
people, as ash is indicative of the amount of minerals contained in any food sample.
European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 47 www.idpublications.org
The fat content (%) of the snack followed the same trends with crude protein, ash and crude
fibre, though the increase in values were minimal. The highest value of 1.68 was recorded for
the 15% pigeon pea substitution while the lowest value of 1.53 at 5% substitution.
Table 1. Proximate composition of Breadfruit flour (BF), Pigeon pea flour (PF) and the
Snacks.
Components
g/100g
BF
PF
Snacks % Addition PF to BF
5
10
15
Moisture
Crude protein
Ash
Crude fibre
Crude Fat
Total
carbohydrate
6.78
5.49
3.06
2.93
1.49
80.25
7.20
18.04
7.62
4.10
1.50
61.18
6.20
6.95
3.93
2.05
1.53
79.34
6.44
7.40
4.25
2.35
1.60
77.95
6.70
8.85
4.58
2.66
1.68
75.53
Crude protein:N×6.25
Carbohydrate by difference
Fat plays a significant role in the shelf life of food products and as such relatively high fat
content could be undesirable in baked food products. This is because fat can promote
rancidity in foods, leading to development of unpleasant and odorous compounds
(Ihekoronye and Ngoddy,1985). The moisture content of the snacks ranged between 6.20 and
6.70, with the 15% substitution having the highest value. These values were minimal and
may not have adverse effect on the quality attributes of the products (Esuoso and Bamiro,
1995). The carbohydrate content reduces as expected with increase in pigeon pea addition
Expansion and product bulk density
As the addition of pigeon pea flour to breadfruit flour increases, expansion of snacks
decreased, while products bulk density increased (Table 2). These changes can be related to
changes in the proximate composition of the feed material (Table 1).
The increase in additions of pigeon pea flour to breadfruit resulted in higher WAI and lower
WSI of snacks. These changes can be related to changes in the proximate composition which
increase the total carbohydrate components and decrease non carbohydrate components
(Table 1). Studies on snacks from starch have shown that the major operations occurring
during the process (namely heating in the presence of water and shearing) can impact
structure to the product through the transformation of starch granules by the mechanism of
gelatinization (Stanley and Sefa-Dedeh, 1987). Tester and Morris (1990) reported in the work
on wheat- starch gluten mixtures reported that the lack of protein in raw in raw materials
could make starch gelatinization easier, since there is no material that can compete with
European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 48 www.idpublications.org
starch in water absorption. Singh et. al., (1991) reported that water holding capacity (WHC)
of snacks decreased while water solubility increased with decreasing concentrate of nonfat
dry milk in the blends with corn flour. Thus, on addition of pigeon pea flour to breadfruit
flour, it is expected that the interaction between protein and starch would be more extensive,
thereby reducing water solubility and increasing water absorption. Increasing WAI is
advantageous in determination of the applicability of the snack products for use in situations
that involve water binding (Aguilera et. al., 1990)
Sensory texture of Breadfruit/ pigeon pea snacks
The effect of pigeon pea to breadfruit flour had significant (P<0.05) influence on sensory
texture of the snacks. There was no panelist variation (P<0.05) in their responses to sensory
texture. Snacks produced from blend of 5% PF/95% BF were different (P<0.05) in texture
from other blends (10% PF/90% BF; 15% PF/85% BF). A mean score of 3.4 (crisp to hard)
was given to snacks from 5% PF /95% BF, 4.4 for 10% PF/90% BF snacks (hard to very
hard). Snacks containing 10% PF and 15% PF required higher perceived force to fracture the
product on 1st bite between molars, unlike snacks with 5%PF, which fractured with ease.
Texture of snacks products is one of the most important characteristics affecting consumer
acceptance (Suknark et al., 1998). Texture, unlike color and flavor is used by consumer not
as an indicator of food safety but as an indicator of food quality. Mizubuti et. al., (2000)
reported that no products based on valued textural characteristics especially crispness are
being created with advertisement stressing the enjoyment they provide.
According to Singh et al., (1991), addition of small amounts of proteins (<5%) enhances
textural properties of snack products. An increase of hardness of the snacks products was
exhibited as an increase in shear strength. Table 2 indicated that snacks from blend 5%PF /
95%BF had the highest expansion with lowest product bulk density, which might have
contributed to the desired crisp texture.
There was no difference (p>0.05) in sensory attributes of odor, texture and overall
acceptance for the flavored snacks. Figure 3 shows the mean hedonic scores given to sensory
attributes (color, odor, flavor, texture and overall acceptability) for the flavored snacks
(chocolate, breadrika, breadpea and onion) breadfruit/ pigeon pea flour snacks.
Table 2- Effect of inclusion of Pigeon pea flour on bulk density, water absorption index and
water solubility index of snacks
Pigeon pea
Flour (%)
Expansion
Bulk density
g/ml
WAI g gel/g
Sample
WSI %
0
5
10
15
1.48a
1.35b
1.28c
1.16d
0.25d
0.28c
0.31b
0.34a
4.2d
4.7c
5.0b
6.2a
36.9a
33.6b
31.6c
30.2d
Mean followed by different superscripts in the same columns are different (P<0.05)
European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
ISSN 2059-3058
Progressive Academic Publishing, UK Page 49 www.idpublications.org
Fig 3- Mean (n=25) Hedonic sensory scores for sensory attributes of flavored breadfruit/
pigeon pea flour snacks
CONCLUSION
The study has shown that inclusion of pigeon pea flour to breadfruit flour in the preparation
of snack is a welcome idea, The snacks produced from addition of 5% pigeon pea flour to
breadfruit flour was more preferable. This sample apart from increasing the protein content, it
produced suitable crisp to hard-textured snacks. All snacks with chocolate, vanilla
(breadrika), pineapple (breadpea) and onion were liked moderately to very much in overall
acceptability. The study has also shown that utilization of both breadfruit and pigeon pea can
be increased and encouraged.
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European Journal of Basic and Applied Sciences Vol. 2 No. 2, 2015
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Progressive Academic Publishing, UK Page 50 www.idpublications.org
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