Running is a modality that has a large number of adepts, including women. Therefore, it is important to understand how sportswear can help women, with special attention to the breast movement. The aim of this study was to analyse the effect of different combinations of breast support and footwear on the breast movement during walking and running. Twenty women performed treadmill walking (5 km/h) and running (7 and 10 km/h) combining different footwear (barefoot, minimalist, and traditional) and breast support conditions (bare breast, everyday bra, and sports bra). Three-dimensional data from breast and trunk markers were tracked for 10 stride cycles. Relative breast displacement was calculated and derived for velocity. An interaction effect was observed between support, footwear, and speed conditions. The bare breast conditions presented differences from the other conditions in the majority of the kinematic variables, presenting higher breast displacement and velocity values. On the other hand, the sports bra conditions presented the lowest values for the kinematics variables. In the vertical component of breast displacement during running (10 km/h) we verified that the sports bra presented reductions of 56% and 43% in relation to the bare breast and everyday bra conditions, respectively. Despite this, no differences were found between footwear within each breast support condition. A sports bra is efficient to decrease breast movement. In addition, neither of the tested footwear was able to decrease these movements.