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Objective: Present study examines predictors of the overall race time and disciplines in the Olympic distance triathlon. Methods: Thirty-nine male and six female triathletes were evaluated for anthropometric, physiological, genetic, training, clinical and circadian characteristics. Body composition, maximum capacity for oxygen uptake (V˙O2max), maximum aerobic velocity (MAV), anaerobic threshold (AT), triathlon experience (TE) and XX genotype for α-actinin 3 affected total race time (p<0.05). Results: Total race time can be predicted by MAV (ß = -0.430, t = -3.225, p = 0.003), TE (ß = -0.378, t = -3.605, p = 0.001), and percentage of lean mass (%LM) (ß = -0.332, t = -2.503, p = 0.017). Swimming can be predicted by MAV (ß = -0.403, t = -3.239, p = 0.002), TE (ß = -0.339, t = -2.876, p = 0.007), and AT%V˙O2max (ß = 0.281, t = 2.278, p = 0.028). Cycling can be predicted by MAV (ß = -0.341, t = -2.333, p = 0.025), TE (ß = -0.363, t = -3.172, p = 0.003), and %LM (ß = -0.326, t = -2.265, p = 0.029). In running split, MAV (ß = -0.768, t = -6.222, p < 0.001) was the only parameter present in the best multiple linear regression model. Conclusion: The most important variables in multiple regression models for estimating performance were MAV, TE, AT and %LM.

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... The variables associated with triathlon performance have previously been studied. Therefore, prediction equations for triathlon performance have also been developed; however, previous studies have only included male athletes, or when female athletes were included, the sex was not evaluated as a biological variable [9,10]. The knowledge of the main predictive variables of performance for each sex separately, can help coaches in directing the training sessions to obtain adaptive responses of the most important predictive variables of performance and optimize the improvement of sports performance. ...
... The G*Power version 3.1.9.2 (Franz, Universität Kiel, Germany) was used to determine the sample size and analyze the test power level. A sample size calculation for regression analysis for overall race time with two predictors, using previous published data from Puccinelli et al. [10] (r 2 = 0.607), showed that 20 athletes were needed to detect a relevant difference with 80% power and a significance level of 5%. The powers of the analyses were also calculated. ...
... Although the literature agrees that men have higher maximum oxygen consumption values [8], data on VTs are contradictory. Puccinelli et al. [10] discovered that female athletes had a higher percentage ofVO 2 max at VT and RCP than male athletes. These contradictory findings could be attributed to the varying levels of training of the athletes. ...
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... This was largely due to the improvement in movement biomechanics and body composition. Puccinelli et al. [27] showed that body composition (as well as previous experience and aerobic capacity) is a good predictor for Olympic triathlon-distance performance in amateurs. Similar results were noted by other authors in various disciplines [28,29]. ...
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Article
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Genetic polymorphism is suggested to be associated with human physical performance. The angiotensin I-converting enzyme insertion/deletion (ACE I/D) polymorphism and the α-actinin-3 gene (ACTN3) R577X polymorphism have been most widely studied for such association analysis. However, the findings are frequently heterogeneous. We aim to summarize the associations of ACE I/D and ACTN3 R577X with sport performance by means of meta-analysis. We systematically reviewed and quantitatively summarized published studies, until October 31, 2012, on relationship between ACE/ACTN3 genetic polymorphisms and sports performance, respectively. A total of 366 articles on ACE and 88 articles on ACTN3 were achieved by literature search. A significant association was found for ACE II genotype compared to D allele carriage (DD+ID) with increased possibility of physical performance (OR, 1.23; 95% CI, 1.05-1.45). With respect to sport discipline, the II genotype was found to be associated with performance in endurance athletes (OR, 1.35; 95% CI, 1.17-1.55). On the other hand, no significant association was observed for ACTN3 RR genotype as compared to X allele carriage (XX+RX) (OR, 1.03; 95% CI, 0.92-1.15). However, when restricted the analyses to power events, a significant association was observed (OR, 1.21; 95% CI, 1.03-1.42). Our results provide more solid evidence for the associations between ACE II genotype and endurance events and between ACTN3 R allele and power events. The findings suggest that the genetic profiles might influence human physical performance.
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Angiotensin I-converting enzyme (ACE) is recognized as one of the main effector molecules involved in blood pressure regulation. In the last few years some polymorphisms of ACE such as the insertion/deletion (I/D) polymorphism have been described, but their physiologic relevance is poorly understood. In addition, few studies investigated if the specific activity of ACE domain is related to the I/D polymorphism and if it can affect other systems. The aim of this study was to establish a biochemical and functional characterization of the I/D polymorphism and correlate this with the corresponding ACE activity. For this purpose, 119 male brazilian army recruits were genotyped and their ACE plasma activities evaluated from the C- and N-terminal catalytic domains using fluorescence resonance energy transfer (FRET) peptides, specific for the C-domain (Abz-LFK(Dnp)OH), N-domain (Abz-SDK(Dnp)P-OH) and both C- and N-domains (Abz-FRK(Dnp)P-OH). Plasma kallikrein activity was measured using Z-Phe-Arg-AMC as substrate and inhibited by selective plasma kallikrein inhibitor (PKSI). Some physiological parameters previously described related to the I/D polymorphism such as handgrip strength, blood pressure, heart rate and BMI were also evaluated. The genotype distribution was II n=27, ID n=64 and DD n=28. Total plasma ACE activity of both domains in II individuals was significantly lower in comparison to ID and DD. This pattern was also observed for C- and N-domain activities. Difference between ID and DD subjects was observed only with the N-domain specific substrate. Blood pressure, heart rate, handgrip strength and BMI were similar among the genotypes. This polymorphism also affected the plasma kallikrein activity and DD group presents high activity level. Thus, our data demonstrate that the I/D ACE polymorphism affects differently both ACE domains without effects on handgrip strength. Moreover, this polymorphism influences the kallikrein-kinin system of normotensive individuals.
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Whether the Met235Thr (rs699) variation in the angiotensinogen (AGT) gene, encoding a threonine instead of a methionine in codon 235 of the mature protein, is associated with athletic performance remains to be elucidated. We compared the genotype and allele frequencies for the AGT Met235Thr variation (rs699) in 119 nonathletic controls, 100 world-class endurance athletes (professional cyclists, Olympic-class runners), and 63 power athletes (top-level jumpers, throwers, sprinters). Participants were all males and from the same descent (Caucasian) for > or =3 generations. The proportion of the CC genotype was significantly higher in the power group (34.9%) than in either the control (16%) or the endurance group (16%) (p = 0.008 and p = 0.005, respectively). The odds ratio (95% CI) of being a power athlete if the subject has a CC genotype was 1.681 (1.176-2.401), compared with the control group. In summary, the C allele of the AGT Met235Thr polymorphism might favour power sports performance. Although more research is needed, this could be attributed to the higher activity of angiotensin II, a skeletal muscle growth factor.
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An English language self-assessment Morningness-Eveningness questionnaire is presented and evaluated against individual differences in the circadian vatiation of oral temperature. 48 subjects falling into Morning, Evening and Intermediate type categories regularly took their temperature. Circadian peak time were identified from the smoothed temperature curves of each subject. Results showed that Morning types and a significantly earlier peak time than Evening types and tended to have a higher daytime temperature and lower post peak temperature. The Intermediate type had temperatures between those of the other groups. Although no significant differences in sleep lengths were found between the three types, Morning types retired and arose significantly earlier than Evening types. Whilst these time significatly correlated with peak time, the questionnaire showed a higher peak time correlation. Although sleep habits are an important déterminant of peak time there are other contibutory factors, and these appear to be partly covered by the questionnaire. Although the questionnaire appears to be valid, further evaluation using a wider subject population is required.
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When running indoors on a treadmill, the lack of air resistance results in a lower energy cost compared with running outdoors at the same velocity. A slight incline of the treadmill gradient can be used to increase the energy cost in compensation. The aim of this study was to determine the treadmill gradient that most accurately reflects the energy cost of outdoor running. Nine trained male runners, thoroughly habituated to treadmill running, ran for 6 min at six different velocities (2.92, 3.33, 3.75, 4.17, 4.58 and 5.0 m s-1) with 6 min recovery between runs. This routine was repeated six times, five times on a treadmill set at different grades (0%, 0%, 1%, 2%, 3%) and once outdoors along a level road. Duplicate collections of expired air were taken during the final 2 min of each run to determine oxygen consumption. The repeatability of the methodology was confirmed by high correlations (r = 0.99) and non-significant differences between the duplicate expired air collections and between the repeated runs at 0% grade. The relationship between oxygen uptake (VO2) and velocity for each grade was highly linear (r > 0.99). At the two lowest velocities, VO2 during road running was not significantly different from treadmill running at 0% or 1% grade, but was significantly less than 2% and 3% grade. For 3.75 m s-1, the VO2 during road running was significantly different from treadmill running at 0%, 2% and 3% grades but not from 1% grade. For 4.17 and 4.58 m s-1, the VO2 during road running was not significantly different from that at 1% or 2% grade but was significantly greater than 0% grade and significantly less than 3% grade. At 5.0 m s-1, the VO2 for road running fell between the VO2 value for 1% and 2% grade treadmill running but was not significantly different from any of the treadmill grade conditions. This study demonstrates equality of the energetic cost of treadmill and outdoor running with the use of a 1% treadmill grade over a duration of approximately 5 min and at velocities between 2.92 and 5.0 m s-1.
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Performance is related to body morphology in many sports. With triathlon making its debut into the Olympic programme in 2000, it was deemed important to determine which physical characteristics of elite-level triathletes were significantly related to performance. Seventy-one elite and junior elite triathletes, from 11 nations, competing at the 1997 World Triathlon Championships were measured on a battery of 28 anthropometric dimensions. A factor analysis was conducted, which reduced the number of variables to four and these were used in a stepwise linear regression to determine which morphological factors were important to performance. Elite triathletes were significantly (p < 0.05) faster than their junior counterparts (males 1:52:26 vs. 2:03:23 and females 2:07:01 vs. 2:14:05) and showed less variation in performance times. Run time variation was the largest of the component disciplines and tended to show the importance of this discipline to the final outcome. Following a factor analysis the four distinguishable morphological factors that emerged were: robustness, adiposity, segmental lengths and skeletal mass. Relating these factors to the total time obtained by the triathletes in this study yielded a regression equation that correlated significantly with all triathletes, accounting for 47% of the variance in total triathlon duration. The regression equations illustrated the importance of low levels of adiposity for elite triathletes for total time and most of the subdisciplines. The other factor that showed importance was that proportionally longer segmental lengths contributed to successful swimming outcome.
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Maximal heart rate (HRmax)-prediction equations based on a person's age are frequently used in prescribing exercise intensity and other clinical applications. Results from various cross-sectional studies have shown a linear decrease in HRmax during exercise with increasing age. However, it is less well established that longitudinal tracking of the same individuals' HRmax as they age exhibits an identical linear relationship. This study examined the longitudinal relationship between age and HRmax during exercise. A retrospective analysis of maximal graded exercise test (GXT) results for members participating in a university-based health-assessment/fitness center between 1978 and 2003 was undertaken in 2006. Records were examined from individuals (N = 132) of both sexes who represented a broad range of age and fitness levels and who had multiple GXT (total N = 908) conducted over 25 years. HRmax-prediction equations based on participants' age and HRmax elicited during the tests were developed using a linear mixed-models statistical analysis approach. Clinical measurements obtained during the administration of the GXT included in this longitudinal study resulted in the generation of a univariate prediction model: HRmax = 207 - 0.7 x age. Model parameters were highly statistically significant (P < 0.001). The relationship between age and HRmax during exercise developed in this longitudinal study has resulted in a prediction equation appreciably different from the conventional HRmax formula (220 - age) often used in exercise prescription, and it confirms findings from recent cross-sectional investigations of HRmax.
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Performance in triathlon is dependent upon factors that include somatotype, physiological capacity, technical proficiency and race strategy. Given the multidisciplinary nature of triathlon and the interaction between each of the three race components, the identification of target split times that can be used to inform the design of training plans and race pacing strategies is a complex task. The present study uses machine learning techniques to analyse a large database of performances in Olympic distance triathlons (2008-2012). The analysis reveals patterns of performance in five components of triathlon (three race "legs" and two transitions) and the complex relationships between performance in each component and overall performance in a race. The results provide three perspectives on the relationship between performance in each component of triathlon and the final placing in a race. These perspectives allow the identification of target split times that are required to achieve a certain final place in a race and the opportunity to make evidence-based decisions about race tactics in order to optimise performance.
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Recently, a fully automated method for segmenting abdominal fat into subcutaneous fat and visceral fat (VF) within the android region using dual-energy X-ray absorptiometry (DXA) has been newly developed. The purpose of this study was to validate a new DXA method by comparing it to the gold standard, computed tomography (CT), in the Korean population. Furthermore, we examined its reliability regarding VF and explored whether it is applicable to the Asian population, which has relatively larger amount of VF. We studied 123 adult men and women, aged 22-73 yr, representing a wide range of body mass index values (17.1-36.0 kg/m(2)), who underwent both DXA and CT in a fasting state within 1 h. The coefficient of determination (r(2)) for regression of the CT on DXA values was 0.892 for females, 0.838 for males, and 0.931 combined. Bland-Altman bias was -451.4 cm(3) with 95% limits of agreement of 149.8 to -1052.6 cm(3). The DXA-measured VF showed a better correlation with CT-measured VF volume than common indices for adiposity, including the body mass index and waist circumference. There was a lesser discrepancy ratio between DXA- and CT-measured VF with an increase of VF volume. DXA, a relatively convenient, inexpensive, and safe method with minimum radiation dosage, can be a reliable technique for VF assessment in the Asian population. Copyright © 2015 The International Society for Clinical Densitometry. Published by Elsevier Inc. All rights reserved.
Article
Oxygen cost (OC) is commonly employed to assess an athlete's running economy, though the validity of this measure is often overlooked. This study evaluated the validity of OC as a measure of running economy by comparison to the underlying energy cost (EC). Additionally, the most appropriate method of removing the influence of body mass was determined to elucidate a measure of running economy that enables valid inter-individual comparisons. 172 highly trained endurance runners (males, n = 101; females, n = 71) performed a discontinuous submaximal running assessment, consisting of approximately seven 3 min stages (1 km [BULLET OPERATOR] hr increments), to determine the absolute OC (L [BULLET OPERATOR] km) and EC (Kcal [BULLET OPERATOR] km) for the 4 speeds below lactate turnpoint. Comparisons between models revealed linear ratio scaling to be a more suitable method than power function scaling for removing the influence of body mass for both EC (R; males, 0.589 vs 0.588; females, 0.498 vs 0.482) and OC (males, 0.657 vs 0.652; females, 0.532 vs 0.531). There were stepwise increases in EC and RER with increments in running speed (both, P < 0.001). However, no differences were observed for OC across the 4 monitored speeds (P = 0.54). Whilst EC increased with running speed, OC was insensitive to changes in running speed, and therefore does not appear to provide a valid index of the underlying EC of running, likely due to the inability of OC to account for variations in substrate utilisation. Therefore, EC should be employed as the primary measure of running economy, and for runners an appropriate scaling with body mass is recommended.
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The swim section of Sprint- and Olympic-distance triathlon race formats is integral to the success of subsequent cycle and running disciplines, and to overall race performance. The current body of swimming-based triathlon research suggests that the energy used, and the positioning gained among competitors during the swim, is important in determining the success of an athlete's race, especially professional athletes in draft-legal settings. Furthermore, by swimming at a reduced intensity, it has been shown that the performance of the subsequent disciplines may be enhanced. However, reductions in energy output can be obtained without compromising swimming speed. This review highlights the importance of swimming intensity during a triathlon and how it impacts on the ensuing cycle and run. Furthermore, consideration is given to current methods used to manipulate swimming performance.
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Previous research (study 1) has shown that a significant relationship exists between 10 km run time (RT) and predicted running velocity at VO2max (vVO2max) among well-trained males heterogeneous in VO2max. Since competitive runners often display a homogeneous fitness profile, the purpose of this study was to determine the association between 10 km RT and vVO2max among a group of trained runners exhibiting nearly identical VO2max values (study 2). Running economy (RE), vVO2max, and velocity at a 4 mM blood lactate concentration (v at 4 mM BL) were calculated in both studies. Correlations were obtained as shown in Table 2. The relationship between VO2max and 10 km RT achieved statistical significance only in study 1, while RE explained a greater amount of performance variation in study 2. In both studies, variation in 10 km RT attributable to vVO2max was similar and exceeded that due to either VO2max or RE. vVO2max also accounted for essentially the same amount of variation in 10 km RT as did v at 4 mM BL. It was concluded that, among well-trained subjects homogeneous in VO2max, a strong relationship exists between 10 km RT and vVO2max that appears to be mediated to a large extent by RE.
Article
The present study was carried out in order to quantify the athlete's endurance impairment after two out of three sequential events of Olympic Triathlon (OT). Furthermore the significance of ventilatory threshold (Tvent) and peak of oxygen uptake (VO2peak) as triathlete's performance predictors was assessed. Tvent and VO2peak were measured in six male triathletes performing an incremental treadmill test a week before an ad hoc triathlon event. The same test was applied immediately after the first two segments of the triathlon (1.5 km swim, 32 km bike). VO2peak and Tvent measured during the latter test were reduced compared to the first test. VO2peak decreased from 69 to 64 ml.kg-1.min-1 and Tvent from 58 to 51 ml.kg-1.min-1 p < 0.01), respectively. VO2peak and Tvent measured in the first test were well correlated (P < 0.05) to both running and cycling times. The Tvent measured during the second test was related to the running time but with a higher significance (p < 0.01) than in the first test. The impairment in the endurance performance induced by the first two segments of OT is an important aspect to consider both in training and in race strategy. These results also provide evidence that VO2peak and Tvent are good predictors of triathlon performance at least in cycling and running events.
Article
Most studies of the triathlon have looked at male triathletes with respect to the longer distance events such as the Hawaii Ironman Triathlon. The purpose of this study was to investigate and compare the physiological characteristics and training protocols of elite and competitive (club) women triathletes who compete at Olympic distance (1.5 km swim, 40 km cycle, 10 km run) to examine the relationship between these factors and running performance in the triathlon. The elite triathletes (n = 10) had a lower body mass and BMI than club level triathletes (n = 9) as well as smaller girths at all measured sites although these differences were not significant. Sum of skinfold thickness measured at four sites was significantly smaller in the elite group (p = 0.05), yet, wide individual variations were found within each group. The amount of training undertaken by individuals in both groups varied markedly; however, overall the elite did more training in all disciplines. The VO2max measured on the treadmill was significantly higher (p = 0.03) in the elite women (65.6 +/- 6.0 ml.kg-1.min-1) as compared to the club level women (60.4 +/- 3.1 ml.kg-1.min-1). The elite triathletes were significantly more economical, showing a lowered relative oxygen consumption (% VO2max), (p = 0.008); lowered heart rate (p = 0.01) and lowered blood lactate values (p = 0.03) at 15 km.h-1. The elite group were also much more efficient runners at 15 km.h-1 when looking at VE/VO2 (p = 0.05). An association was found between run race time and %VO2max at 15 km.h-1 (p = 0.04). The results suggest that there is no ideal nor unique anthropometric profile that can be established for female triathletes especially with respect to running time and overall performance. The widespread differences in physiological variables found between the two groups confirms the important contribution factors such as these make to performance.
Article
The purpose of this study was to investigate if selected physiological variables were related to triathlon performance. Eighteen male and seven female triathletes competed in a short-course triathlon (1-km swim, 30-km cycle, 9-km run) and underwent physiological testing within 14 d. VO2max and ventilatory threshold (VT) were measured on a cycle ergometer, treadmill, and tethered swim apparatus. Leg flexion and extension strength were measured on a Cybex II isokinetic dynamometer. Multiple linear regression did not improve the prediction of triathlon performance over that provided by simple correlations. Swim performance was related to relative swim VO2max in both males (r = -0.48) and females (r = -0.93) as well as the resistance pulled at swim VT (r = -0.81) and absolute leg flexion strength (r = -0.77) in females. No physiological variables were significantly related to cycling time in either gender. Running time was related to relative VO2max (r = -0.88) in females and velocity at run VT in both females (r = -0.88) and males (r = -0.73). Relative swim VO2max (r = -0.98), velocity at run VT (r = -0.89), and absolute leg flexion strength (r = -0.80) were related to overall performance in female triathletes. The only significant predictor of overall triathlon time for males was velocity at run VT (r = -0.78). It therefore appears that in short-course triathletes physiological variables in swimming and running are important to overall performance. Differences in sample size, group variability, and level of performance between males and females may account for the reported differences in the physiological predictors of performance between genders.
Article
The physiological demands of sequential exercise in swimming, cycling and running are unique and require the triathlete to develop physical and physiological characteristics that are a blend of those seen in endurance swimming, cycling and running specialists. Elite triathletes are generally tall, of average to light weight and have low levels of body fat, a physique which provides the advantages of large leverage and an optimal power to surface area or weight ratio. Triathletes have high maximum oxygen uptake (V̇O2max) values, but V̇O2max may be on average marginally lower than values previously observed in endurance specialists. Although V̇O2max is a predictor of performance in triathletes of mixed abilities, it cannot be used to predict performance within homogenous groups of elite performers. Nevertheless, elite triathletes have significantly higher V̇O2max values than sub-elite triathletes and high V̇O2max levels are required for success in triathlons. The ability of the triathlete to exercise at a lower percentage of V̇O2max for a given submaximal workload may be especially important to triathlon success. This is influenced not only by V̇O2max itself, but also by anaerobic threshold and economy of movement. Anaerobic threshold, as indicated by either ventilatory threshold or lactate threshold, improves with triathlon training and when measured in the appropriate exercise mode has been related to swim, cycle and run performance in the triathlon. Economy of movement in swimming, cycling and running is also related to triathlon performance, and swimming economy in particular appears to be an area where triathletes could make large improvements. Future research should utilise experimental methodologies to investigate triathlon physiology, in particular, the influence of sequential exercise in different exercise modes on physiological function and examine the influence of different training interventions on triathlon physiology and performance.
Article
In the exercising human, maximal oxygen uptake (VO2max) is limited by the ability of the cardiorespiratory system to deliver oxygen to the exercising muscles. This is shown by three major lines of evidence: 1) when oxygen delivery is altered (by blood doping, hypoxia, or beta-blockade), VO2max changes accordingly; 2) the increase in VO2max with training results primarily from an increase in maximal cardiac output (not an increase in the a-v O2 difference); and 3) when a small muscle mass is overperfused during exercise, it has an extremely high capacity for consuming oxygen. Thus, O2 delivery, not skeletal muscle O2 extraction, is viewed as the primary limiting factor for VO2max in exercising humans. Metabolic adaptations in skeletal muscle are, however, critical for improving submaximal endurance performance. Endurance training causes an increase in mitochondrial enzyme activities, which improves performance by enhancing fat oxidation and decreasing lactic acid accumulation at a given VO2. VO2max is an important variable that sets the upper limit for endurance performance (an athlete cannot operate above 100% VO2max, for extended periods). Running economy and fractional utilization of VO2max also affect endurance performance. The speed at lactate threshold (LT) integrates all three of these variables and is the best physiological predictor of distance running performance.
Article
Four days after competing in an Olympic-distance National Triathlon Championship (1500-m swim, 40-km cycle, 10-km run), five male and five female triathletes underwent comprehensive physiological testing in an attempt to determine which physiological variables accurately predict triathlon race time. All triathletes underwent maximal swimming tests over 25 and 400 m, the determination of peak sustained power output (PPO) and peak oxygen uptake (VO2peak) during an incremental cycle test to exhaustion, and a maximal treadmill running test to assess peak running velocity and VO2peak. In addition, submaximal steady-state measures of oxygen uptake (VO2), blood [lactate], and heart rate (HR) were determined during the cycling and running tests. The five most significant (P < 0.01) predictors of triathlon performance were blood lactate measured during steady-state cycling at a workload of 4 W x kg(-1) body mass (BM) (r = 0.92), blood lactate while running at 15 km x h(-1) (r = 0.89), PPO (r = 0.86), peak treadmill running velocity (r = 0.85), and VO2peak during cycling (r = 0.85). Stepwise multiple regression analysis revealed a highly significant (r = 0.90, P < 0.001) relationship between predicted race time (from laboratory measures) and actual race time, from the following calculation: race time (s) = - 129 (peak treadmill velocity [km x h(-1)]) + 122 ([lactate] at 4 W x kg(-1) BM) + 9456. The results of this study show that race time for top triathletes competing over the Olympic distance can be accurately predicted from the results of maximal and submaximal laboratory measures.
Article
We have previously shown that the insertion allele of the angiotensin-converting enzyme (ACE) gene was over-represented in the fastest South-African-born finishers of the South African Ironman Triathlons. As ACE is a component of the skeletal muscle kallikrein-kinin system (KKS), the aim of this study is to determine if there are any further associations between polymorphisms within the BDKRB2 and NOS3 genes, which encode for the KKS components, bradykinin beta(2) receptor and nitric oxide synthase, respectively, and ultra-endurance performance during the Ironman Triathlons. Four-hundred and forty-three male Caucasian triathletes who completed the 2000 and/or 2001 South African Ironman Triathlons and 203 healthy Caucasian male control subjects were genotyped for the functional -9/+9 polymorphism within exon 1 of the BDKRB2 gene and the G894T NOS3 gene polymorphisms. The BDKRB2 -9/-9 genotype occurred at a significantly higher frequency when the triathlete group (27.0%) was compared with the control group (19.3%, P=0.035). When divided into tertiles, there was also a significant linear trend for the NOS3 GG genotype distribution among the fastest (35.0%), middle (40.4%) and slowest (46.9%) finishers (P=0.039). The overall finishing times of the triathletes with an NOS3 GG genotype together with a BDKRB2 +9 allele were significantly slower than those with other genotype combinations (P=0.001). The NOS3/BDKRB2 genotype (beta=-0.150, B=-31.48, P=0.002), together with body mass index and age, accounted for 14.6% of the variance in the overall race time for the triathlon. In conclusion, both the NOS3 and BDKRB2 genes are associated with the actual performance during the Ironman Triathlons.
Heart rate response during a simulated Olympic boxing match is predominantly above ventilatory threshold 2: a cross sectional study
  • Cab De Lira
  • Peixinho-Pena
  • Vancini R L
  • Fachina
  • A A De Almeida
  • S Andrade M Dos
de Lira CAB, Peixinho-Pena, Vancini R l, Fachina, de Almeida AA, Andrade M dos S, et al. Heart rate response during a simulated Olympic boxing match is predominantly above ventilatory threshold 2: a cross sectional study. Open Access J. Sports Med. 2013:175. https://doi.org/10.2147/oajsm.s44807.
ACSM's Guidelines for Exercise Testing and Prescription
American College of Sports Medicine, ACSM's Guidelines for Exercise Testing and Prescription, Lippincott Williams & Wilkins, 2013.