Lectins are glycoproteins connected to starch noncovalently, a novel lectin was refined from the wild mushroom Terfezia claveryi. This lectin likewise exhibited hemagglutinating action. Cleansing of lectin was practiced by 45% immersion ammonium sulfate pursued by particle trade chromatography on DEAE cellulose section at that point gel filtration chromatography Sephadex G100 with decontamination crease 2.793, atomic weight 45KDa, it is an acidic protein with the most noteworthy solidness at pH 6.5. Divalent particles did not influence its action and it was steady under warming till 80oC.The antibacterial action of Terfezia claveryi lectin against P.aeruginosa disconnects was considered. Unrefined and purged Terfezia claveryi lectin were exposed to antibacterial movement against Pseudomonas aeruginosa pathogen. The outcomes indicated that Terfezia claveryi lectin at fixations, 100 and 50 µg/ml hold good antibacterial activity against P. aeruginosa confines as contrasted and control (P<0.05). The antibacterial activity of cleaned and unrefined Terfezia claveryi lectin in focus 100 µg/ml was higher than an antibacterial activity of fixation 50 µg/ml (P<0.05). Furthermore, the antibacterial activity of purified Terfezia claveryi lectin was significantly higher than crude, P<0.05. The half leaf method was applied to find out the antiviral action of Terfezia claveryi lectin toward TMV in vitro. TMV infection could also be inhibited by this lectin. It potentially can be used for developing anti-phyto-viral agents to control plant diseases and also represents a useful addition to the present consortium of mushroom lect. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to study the cytotoxicity effect of Terfezia claveryi lectin on some cancer and normal human cell line. The results showed the cytotoxicity effect on liver hepatocellular cancer cell line compared with normal embryonic liver cell line, the use of lectins obtained from natural plant source shows great promise and potential for use in future cancer therapy.