Since from the recent past, the attention of industrial wastes has grabbed the focus of the researchers toward designing and development of certain methods or processes in order to lessen the usage and the production of pollutants. With the knowledge of green chemistry, the catalysis using enzymes is the potential method for the production of industrial polymers at a high level without using hazardous reagents and thus curbing the pollution. Enzyme cofactors play a significant role in bio-catalysis since enzymes need them in order to catalyze significantly important reactions in organic synthesis. With the introduction and implementation of stringent processes to discharge waste into the environment, there is a need of the hour to develop alternative methods or processes to treat wastes. There exist large numbers of reported enzymes, which are mentioned in this chapter, from numerous microbes which are playing a major role in treating industrial wastes. Enzymes being specific in nature act and remove pollutants of recalcitrant nature via a certain process like precipitation and then transforming to some other products with certain changes in their characteristics. This results in increasing the susceptibility toward treatment in order to transform the waste into a value-added product. There are numerous unique applications of the catalytic activities, highlighted in the chapter for the proper understanding of the enzymes with the significance of their functions. Nowadays, it has become extensively important to use enzymes rather than hazardous chemicals so as to touch the demands of healthy, clean, and green technologies to protect our earth. This chapter highlights the importance of bio-catalytic reactions to treat various industrial wastes like the tobacco industry, pharmaceutical industry, textile industry, and dye industry with minimum invasiveness.