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Medicinal plant Allium sativum = A Review


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Garlic Allium sativum the Liliaceae family, is among the oldest of all cultivated plants. It has been used as a medicinal agent for thousands of years. Garlic is one of the most important bulb vegetables, which is used as spice and flavoring agent for foods Garlic adds to taste of foods as well as it helps to make them digestible. Garlic contains different useful minerals, vitamins and many other substances used for health of human beings. It is rich in sugar, protein, fat, calcium, potassium, phosphorous, sulfur, iodine fiber and silicon in addition to vitamins. It possesses high nutritive value. Furthermore, garlic has pharmaceutical effects and used to cure a vast conditions including blood pressure and cholesterol, cancer, hepatoprotective, anthelmintics, anti-inflammatory, antioxidant, antifungal and wound healing, asthma, arthritis, sciatica, lumbago, backache, bronchitis, chronic fever, tuberculosis, rhinitis, malaria, obstinate skin disease including leprosy, leucoderma, discoloration of the skin and itches, indigestion, colic pain, enlargement of spleen, piles, fistula, fracture of bone, gout, urinary diseases, diabetes, kidney stone, anemia, jaundice, epilepsy, cataract and night blindness. Garlic products are used as sources of medicine in many ways in human beings in their day today life. As a result, researchers from various disciplines are now directing their efforts towards discovering the medicinal values of garlic on human health. The main interest of researchers in the medicinal values of garlic is its broad-spectrum therapeutic effect with minimal toxicity.
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Journal of Medicinal Plants Studies 2016; 4(6): 72-79
ISSN 2320-3862
JMPS 2016; 4(6): 72-79
© 2016 JMPS
Received: 11-09-2016
Accepted: 12-10-2016
Dr. Md Khorshed Alam
Hakim Said Eastern Medical
College and Hospital, Dhaka,
Dr. Md Obydul Hoq
Hakim Said Eastern Medical
College and Hospital, Dhaka,
Dr. Md Shahab Uddin
Hakim Said Eastern Medical
College and Hospital, Dhaka,
Dr. Md Khorshed Alam
Hakim Said Eastern Medical
College and Hospital, Dhaka,
Medicinal plant Allium sativum = A Review
Dr. Md Khorshed Alam, Dr. Md Obydul Hoq and Dr. Md Shahab Uddin
Garlic Allium sativum the Liliaceae family, is among the oldest of all cultivated plants. It has been used
as a medicinal agent for thousands of years. Garlic is one of the most important bulb vegetables, which is
used as spice and flavoring agent for foods Garlic adds to taste of foods as well as it helps to make them
digestible. Garlic contains different useful minerals, vitamins and many other substances used for health
of human beings. It is rich in sugar, protein, fat, calcium, potassium, phosphorous, sulfur, iodine fiber and
silicon in addition to vitamins. It possesses high nutritive value. Furthermore, garlic has pharmaceutical
effects and used to cure a vast conditions including blood pressure and cholesterol, cancer,
hepatoprotective, anthelmintics, anti-inflammatory, antioxidant, antifungal and wound healing, asthma,
arthritis, sciatica, lumbago, backache, bronchitis, chronic fever, tuberculosis, rhinitis, malaria, obstinate
skin disease including leprosy, leucoderma, discoloration of the skin and itches, indigestion, colic pain,
enlargement of spleen, piles, fistula, fracture of bone, gout, urinary diseases, diabetes, kidney stone,
anemia, jaundice, epilepsy, cataract and night blindness. Garlic products are used as sources of medicine
in many ways in human beings in their day today life. As a result, researchers from various disciplines
are now directing their efforts towards discovering the medicinal values of garlic on human health. The
main interest of researchers in the medicinal values of garlic is its broad-spectrum therapeutic effect with
minimal toxicity.
Keywords: Garlic, medicinal plant, allicin, antihypertensive, antidiabetic. antiatherosclerosis
1. Introduction
Garlic (Allium sativum) is among the oldest of all cultivated plants. It has been used as a spice,
food and folklore medicine for over 4000 years, and is the most widely researched medicinal
plant (Milner 1996) [35]. Codex Ebers, an Egyptian medical papyrus dating to about 1550 B.C.,
includes 22 therapeutic formulations that mention garlic as an effective remedy for a variety of
ailments including heart problems, headache, bites, worms and tumors (Block 1985) [8].
According to the Bible, the Jewish slaves in Egypt were fed garlic and other allium vegetables,
apparently to give them strength and to increase their productivity (Rivlin 2001) [48]. In ancient
Greece, garlic was consumed to treat intestinal and lung disorders (Farbman et al. 1993) [21]. In
India, garlic has been used for centuries as an antiseptic lotion for washing wounds and ulcers.
During World War II, garlic was used to treat the wounds of soldiers (Essman 1984) [18]. Many
workers have researched on garlic’s insecticidal, antimicrobial, antiprotozoal and antitumor
activities (Bolton et al; 1982) [9]. In traditional Chinese medicine, Islamic medicine, folklore
medicine and the Ayurvedic system of medicine, several spices and herbs including garlic are
described to possess medicinal properties e.g. anti-thrombotic, hypolipidemic and anti-
hypertensive (Makheja 1990 and Moyers 1996) [36, 37]. In the homeopathic system, garlic is
also an effective remedy for many ailments. In China, garlic tea has long been recommended
for fever, headache, cholera and dysentery. In rural Japan, miso-soup containing garlic is used
as a remedy for the common cold with headache, fever and sore throat (Sato et al. 2000) [53].
More recently, garlic has been reported to be effective in various ailments such as
cardiovascular diseases because of its ability to lower serum cholesterol (Bordia et al. 1977)
[10]. A component of garlic, S-methylcysteine sulfoxide (SMCS), has been shown to reduce
both blood cholesterol and the severity of atherosclerosis (Sainani et al. 1979) [54]. Garlic has
protective effects against stroke, coronary thrombosis, atherosclerosis, platelet aggregation, as
well as infections and vascular disorders. However, we must note that there is considerable
controversy concerning the cholesterol lowering effects of garlic and a number of studies have
reported that some garlic preparations do not lower serum cholesterol. The fibrinolytic activity
of garlic in both man and experimental animals has been reported.
Journal of Medicinal Plants Studies
Many claims of an antibiotic action, a hypoglycemic effect,
antitumor, antioxidant and antithrombotic properties have also
been attributed to the garlic extracts (Alnaqeeb et al. 1992) [2].
2. Botanical Description
Other members of the onion genus, Allium, include A. cepa
(onion), A. schoenoprasum (chives), A. ascalonicum (shallots),
and A. porrum (leeks). Allium sativum is further divided into
two subspecies, A. sativum var. sativum, also known as
softneck garlic, and Allium sativum var. ophioscorodon, also
known as hardneck garlic. Both varieties are composed of an
underground bulb made up of cloves, which are prophylls
enclosed by dry membranous skins and held together by a
basal plate. The variations differ in that hard neck garlic’s bulb
is composed of six to eleven cloves, circled around a
centralized woody stalk. This variety of garlic has a scape that
curls at the top, but it is generally removed after it curls one to
three times. This is because if it continues to grow, less energy
can be utilized towards the bulb. Eventually, the scape would
give rise to bulbils, containing miniature cloves. The bulbils
are occasionally accompanied by white or light purple flowers,
although these are sterile. Softneck garlic does not have a
flowering top and contains up to twenty-four cloves per bulb.
The stem is central and soft, hence the name, and the cloves
are layered with larger ones on the outside. Allium sativum is
the more common variation, many studies involving garlic do
not specify which subspecies is used, but chemical and
biological action are assumed to be similar. Allium sativum is
sterile and hence is grown asexually from cloves, not requiring
a pollinator. It grows best in mild climates, through hardneck
varieties are better adapted to colder environments. Allium
sativum is a perennial species, as are most members of the
genus. Garlic is composed of very strong organosulfur
compounds that serve as secondary metabolites as described in
the section entitled chemistry and pharmacology. These
compounds are responsible for the very pungent smell and
taste of raw garlic and act as defenses against predators (Block
2010) [11].
2.1 Bulb
Rounded, composed of up to about 15 smaller bulblets known
as cloves. Cloves and bulbs are covered by a whitish or
pinkish tunic (papery coat).
2.2 Leaves
Four to twelve long, sword-shaped leaves attached to an
underground stem.
2.3 Flowers
Borne in a dense, spherical cluster on a spike (flower stalk) up
to 25 cm long. The young flower head is enclosed in a long-
beaked pair of enclosing bracts, which become papery and
split to reveal the flowers. Individual flower stalks arise from a
common point. Flowers are greenish-white or pinkish with six
perianth segments (sepals and petals) about 3 mm long.
Bulbils (asexual propagules), which resemble tiny cloves, are
often interspersed among the flowers.
2.4 Fruits
Flowers usually abort before developing to a stage at which
fertilisation could take place.
2.5 Seeds
Not usually produced in the wild but have been produced
under laboratory conditions. With a black coat, similar to
onion seeds, but approximately half the size.
3. Vernacular Names
Synonyms : Allium sativum L.
Sanskrit : Lasuna, Rosona, Yovanesta
English : Garlic, poorman’s treacle
Bangali : Rosun
Hindi : Lashan, lahsun
Arabic : Saun Taum
German : Knoblauch, Lauch
Greek : Allidion, Skorodon
Italian : Aglio
Chinese : Syun tauh
Urdu : Lehsun
Malayalam : Veluthulli.
4. Unani description:
Unani name : Lehsun, Fum, Thoum, Thum
Botanical name : Allium sativum L.
Synonyms : Allium sativum, Garlic, Saun Taum,
Lehsun, Lashan, lahsun.
Properties : Mizaj 3rd Order Warm and Dry
Maza : Bitter and acrid
Boo : Radio-active and acrid
Muzir : Mehrooreen (for persons with hot
Mukhrij : Expels Balgham (Phlegm)
Nafa-e-Khas : Phlegmatic and stomach related
5. Important formulations
Important Unani formulations containing Allium sativum are as
Garlic parls
Qurch Ziabetis
Sarbat Lahsun (B.N.U.F 2010)
6. Classification of Allium sativum
Kingdom : Plantae
Subkingdom : Tracheobionta
Superdivision : Spermatophyta
Division : Magnoliophyta
Class : Equisetopsida
Subclass : Magnoliidae
Superorder : Lilianae
Order : Asparagales
Family : Amaryllidaceae
Genus : Allium
7. Chemistry and Pharmacology
Sulfur compounds are the main chemical constituents
responsible for Allium sativum’s taste, smell, and likely for its
biological effects. When a garlic clove is intact, glutamyl
cysteins are the primary sulfur components (Powolyny and
Singh 2008) [47]. These are hydrolyzed to form alliin. When
garlic is crushed by chewing, chopping, etc., the alliin
promptly reacts with the enzyme alliinase to form allicin; after
30 seconds the reaction is 97% complete. Allicin is a diallyl
thiosulfinate that accounts for 70-80% of the thiosulfinates
present in Allium sativum (Harunobu et al. 2001) [23]. Allicin is
also highly unstable and quickly decomposes to yield sulfur
compounds when oxidized such as diallyl sulfide (DAS),
diallyl disulfide (DADS), diallyl trisulfide (DATS), ajoene,
and hydrogen sulfide (Banerjee and Maulik 2002) [12]. The
pathways of this reaction can be seen in Figure 1. DAS,
DADS, DATS and ajoene are all oil soluble (Miroddi et al.
2011) [38].
Journal of Medicinal Plants Studies
Fig 1: Chemical structures of organ sulfur compounds involved in reactions with allicin. (Image source: Powolny and Singh 2008) [47].
8. Chemical Manipulation
When garlic is manipulated to produce alternate forms, the
unstable sulfur compounds react and hence alter active
chemical constituents. It is important to note that these
changes in chemistry can alter the bioavailability of the
compounds. Unfortunately, many studies do not specify the
actions taken, which could possibly account for
inconsistencies in reported data. Traditionally used in its raw
form, garlic is now often heated, dehydrated, and aged. Only
freshly crushed garlic has hydrogen sulfide, which is suspected
to have significant cardioprotective effects as a vasodilator
(Mukherjee et al. 2009) [39]. Garlic powder can be made
through dehydrating the plant with heat, but when high
temperatures are applied to garlic, alliinase is deactivated and
hence cannot react with alliin to form allicin (Tsai et al. 2011)
[59]. This explains why cooked garlic has a mellower flavor
than raw garlic. Powder can retain some allicin content if the
cloves are frozen before being pulverized; acetone removes the
water and alliin and alliinase remain separate yet intact until
water is added, at which point allicin is formed. While
dehydration during the powdering process does not destroy
alliinase like heat does, more than half of the alliin is lost.
While levels are lower than with raw garlic, dried garlic does
contain alliin and alliinase, as explained above. Alliinase is
deactivated by the acidic environment of the stomach
(Touloupakis and Ghanotakis 2011) [60]. Freeman and Kodera
(1995) [22] also came to this conclusion by exposing
dehydrated garlic powder to simulations of the gastrointestinal
fluids; allicin production decreased by 99%, presumably due to
the lack of allinase. This suggests that it could be beneficial to
further research the affects of dehydrated garlic powder when
taken in a capsule with an enteric coating to protect it from
stomach acid. These studies also show that manipulating
garlic’s form leads to changes in the active constituents and
could lead to data inconsistencies in studies.
Garlic can also be aged by soaking it in aqueous ethanol and
then extracting and concentrating essential compounds. When
allicin is dissolved in oils, the major compound in the final
product is S-Allylcystein (SAC) while ajoene, the most stable
component of garlic, is also present (Rahman 2002) [49].
Kodera et al. (2002) [29] suggest that SAC could pass through
the gastrointestinal tract without decomposing and
successfully be absorbed. Kodera et al. (2002) [29] also suggest
that SAC might be stable in blood, whereas allicin is unstable
in blood and cannot reach target organs via circulation
(Harunobu et al. 2001) [23].
9. Pharmacological activities
Due to its biological active component allicin and its
derivative, garlic has been used as a medicine to cure a wide
range of diseases and conditions related the heart and blood
system including high blood pressure, high cholesterol,
coronary heart disease, heart attack, and “hardening of the
arteries” (atherosclerosis) as pronounced (Mikaili et al. 2013)
Amagase (2006) [3] noticed out garlic is used to prevent
various types of cancer comprising colon cancer, rectal cancer,
stomach cancer, breast cancer, prostate cancer, prostate cancer
and bladder cancer, and lung cancer. It is also used to treat
Cardiovascular disease including: Antilipemic,
antihypertensive, anti-atherosclerotic, an enlarged prostate
(benign prostatic hyperplasia; BPH), diabetes, osteoarthritis,
hayfever (allergic rhinitis), traveler's diarrhea, high blood
pressure late in pregnancy (pre-eclampsia), cold and flu. It is
also used for building the immune system, preventing tick
bites, and preventing and treating bacterial and fungal
Furthermore, Pendbhaje et al. (2000) [46] listed out the
pharmaceutical activities of garlic. The plant is effective to
treat fever, coughs, headache, stomach ache, sinus congestion,
gout, rheumatism, hemorrhoids, asthma, bronchitis, shortness
of breath, low blood pressure, low blood sugar, high blood
sugar, and snakebites. It is also used for fighting stress and
fatigue, and maintaining healthy liver function. In addition to
this, Jung et al. (2000) [26] reported that garlic is also used to
promising effect against to asthma, arthritis, sciatica, lumbago,
backache, bronchitis, chronic fever, tuberculosis, rhinitis,
malaria, obstinate skin disease including leprosy, leucoderma,
discolouration of the skin and itches, indigestion, colic pain,
enlargement of spleen, piles, fistula, fracture of bone, gout,
urinary diseases, diabetes, kidney stone, anemia, jaundice,
epilepsy, cataract and night blindness. Garlic paly crucial role
in area of pharmaceutical and used for the treatment of
cardiovascular and other demise causing ailments including:
9.1 Antibacterial activity
Garlic is a broad spectrum antibiotic, killing a wide variety of
bacteria. Dr. Tariq Abdullah, a prominent garlic researcher
stated in the August 1987 issue of Prevention: “Garlic has the
broadest spectrum of any antimicrobial substance that we
know of it is antibacterial, antifungal, antiparasitic,
antiprotozoan and antiviral.” This property belongs to the
garlic constituent allicin, which is released when you cut a
Journal of Medicinal Plants Studies
garlic clove. This is the chemical that gives fresh garlic its
strong biting flavor, and you need to use fresh garlic to get a
reliable antibiotic effect. Garlic appears to have antibiotic
activity whether taken internally or applied topically.
Researchers found that the urine and blood serum of human
subjects taking garlic had activity against fungi (Caporaso et
al. 1983) [16].
9.2 Antiviral activity
Garlic and its sulfur constituents verified antiviral activity
against coxsackievirus species, herpes simplex virus types 1
and 2, influenza B, para-influenza virus type 3, vaccinia virus,
vesicular stomatitis virus, human immunodeficiency virus type
1 and human rhinovirus type 2. The order of compounds found
in garlic for virucidal activity was, ajoene > allicin > allyl
methyl thiosulfinate > methyl allyl thiosulfinate; no activity
was found for the polar fractions, alliin, deoxyalliin, diallyl
disulfide, or diallyl trisulfide. Several laboratory tests have
shown that garlic is an effectual treatment for both the
influenza B virus and herpes simplex virus. Two independent
researchers in Japan and Romania have found that garlic is
able to protect living organisms from the influenza virus (Tsai
et al., 1985) [61]. Most recently, a double blind placebo con-
trolled study has shown significant protection from the
common cold virus. As conducted by The Garlic Centre,
published in Advances in Therapy, this is the first serious
work to show prevention, treatment and reduction of re-
infection benefits from taking Allimax Powder capsules once
daily (Josling, 2001) [27].
9.3 Antifungal activity
Ajoene is an active compound found in garlic which plays a
great role as topical antifungal agent (Ledezma and Apitz-
Castro, 2006) [32]. Garlic has been shown to inhibit growth of
fungal diseases as equally as the drug ketoconazole, when
tested on the fungi Malassezia furfur, Candida albicans,
Aspergillus, Cryptococcus and other Candida species (Shams-
Ghahfarokhi et al., 2006) [56]. A report from a Chinese medical
journal delineates the use of intravenous garlic to treat a
potentially fatal and rare fungal infection of the brain called
Cryptococcus meningitis. In the report, the Chinese compared
the effec-tiveness of the garlic with standard medical treatment
which involved a very toxic antibiotic called Amphotericin-B.
The study revealed that, intravenous garlic was more effective
than the drug and was not toxic regardless of its dosage
(Lemar et al., 2007) [33].
A study found that Candida colonies were substantially
reduced in mice that had been treated using liquid garlic
extract. The study also revealed that garlic stimulated
phagocytic activity. This implies that infections such as
Candida may be controlled because garlic stimulates the
bodys own defenses. Garlic oil can be used to treat ring-
worm, skin parasites and warts if it is applied externally.
Lesions that were caused by skin fungi in rabbits and guinea
pigs were treated with external applications of garlic extract
and began to heal after seven days (Sabitha et al., 2005) [57].
9.4 Antiprotozoal activity
Garlic is effective in treating intestinal parasites has been
known for a long time. An extract of garlic was effective
against a host of protozoa such as Opalina ranarum, Opalina
dimidicita, Balantidium entozoon, Entamoeba histolytica,
Trypanosoma, Leishmania, Leptomonas and Crithidia (Reuter
et al, 1996) [50]. In addition, it was efficacious at killing wild-
type amoebae isolated from the diseased fish, slowing the
clinical signs of amoebic gill disease (AGD). However, it is
necessary to study the toxicity and pathological effect of garlic
on Atlantic salmon before using garlic to treat AGD in farmed
Atlantic salmon.
9.5 Antiparasitic activity
Many herbalists worldwide recommend garlic as a treat-ment
for intestinal parasites. In some cultures, children infested with
helminthes are treated with enemas containing crushed garlic.
One of the traditional Chinese medical treatments for intestinal
diseases is an alcoholic extract of crushed garlic cloves.
Allicin exhibits anti-parasitic activity against major human
intestinal parasites such as Entamoeba histolytica, Ascaris
lumbricoides and Giardia lamblia (Kalyesa et al., 1975) [75].
Entamoeba histolytica, the human intestinal protozoan
parasite, is very sensitive to allicin, as only 30 μg/ml of allicin
totally inhibits the growth of amoeba cultures (Mirelman et al.,
1987) [41]. Moreover, researchers have found that at lower
concentrations (5 μg/ml), allicin inhibited 90% the virulence of
trophozoites of E. histolytica as determined by their inability
to destroy mono-layers of tissue-cultured mammalian cells in
vitro (Ankri et al., 1997) [4].
9.6 Wound Healing activity
Successful wound healing depends upon angiogenesis, and
impaired angiogenesis is a hallmark of the chronic wounds
encountered with diabetes and venous or arterial insufficiency.
To intervene and improve wound closure, it is essential to
investigate the effects of different natural remedies in wound
healing. Study was done on the chicken dorsum skin excision
wound assay to investigate the influence of different
concentrations of aged garlic solution (AGS) on wound
healing. Gross, histopathology, scanning electron microscopy
(SEM) and computer-based three-dimensional (3D) image-
probing techniques were utilized to determine the effects of
AGS on wound closure, re-epithelialization, dermal matrix
regeneration and angiogenesis (Jalali et al. 2009) [24].
9.7 Anti- Diabetic activity
A number of animal studies support the effectiveness of garlic
in reducing blood glucose in streptozotocin-induced as well as
alloxan-induced diabetes mellitus in rats and mice. Most of the
studies showed that garlic can reduce blood glucose level in
diabetic mice rats and rabbits. One Iranian study evaluated oral
administration of garlic extract for 14 days on the level of
serum glucose, total cholesterol, triglycerides, urea, uric acid,
creatinine, in normal and streptozotocin-induced diabetic rats.
Administrations of the garlic extract significantly decreased
serum glucose, total cholesterol, triglycerides, urea, uric acid,
creatinine, aspartate amino transferase and alanine amino
transferase levels, while increased serum insulin in diabetic
rats but not in normal rats (p<0.05). Interestingly, a
comparison was made between the action of garlic extract and
glibenclamide, a well-known antidiabetic drug. The
antidiabetic effect of the garlic was more effective than that
observed with glibenclamide (Eidi et al. 2006) [19].
Unfortunately, the effect of garlic on humans with diabetes is
not well studied as is fraught with conflicting results (Zhang et
al. 2001) [65].
9.8 Antihypertensive activity
Garlic powder is used to cure hypertensive. According to
Silagy and Neil (1994) [55] garlic extracts has a significant
reduction in systolic blood pressure (SBP) and in diastolic
blood pressure (DBP) and act as anti-hypertensive.
Journal of Medicinal Plants Studies
9.9 Anti-tumor Effects
Garlic extracts used as inhibition of cancer development in the
presence of known tumor promoters and Sulphurous
components present in garlic are believed to be liable to evade
the developing of cancerous cells in stomach, liver, and other
organs of human as described by Pendbhaje et al. (2000) [46].
9.10 Liver Protective/Detoxification Effects
It has been reported that aged Garlic Extract have liver
protective effects. It has demonstrated in vivo from the liver
toxins: carbon tetrachloride, paracetamol (acetaminophen) and
bromobenzene (Amagase 2000). It has been shown to inhibit
both the formation and bioactivation of liver carcinogenic
nitrosamines and has prevented the mutagenic effects of
aflatoxin B1as pronounced (Borek1998) [14].
9.11 Antioxidative and Radioprotective Effects
Borek (2001) [13] reported that aged garlic extract and its
various constituents have proven an array of antioxidant and
radio-protective effects in studies. They have been shown to
protect white blood cells from radiation damage, liver cells
from lipid peroxidation and vascular endothelial cells from
oxidant injury and enhance antioxidative enzyme systems in
cells. They have been shown to scavenge hydrogen peroxide,
to inhibit the formation of TBA-RS, to protect the heart from
cardiotoxic, anticancer drug doxorubicin, to protect the
kidneys from the antibiotic gentamicin as described by Oshiba
et al (1990) [43].
9.12 Diuretic and Digestive activity
IT has reported that garlic acts as a diuretic which helps to get
rid of body liquids. It may act as a
very useful resource in case of rheumatism, gout, arthritis,
hidropesia, edemas. It eases digestion by stimulating the liver,
the gall bladder and the pancreas although its use should be
avoided when existing hyperchloridia (stomach acidity) and
also when having frail stomachs (Eat it raw or crushed and
mixed with butter) (Ali 1995) [5].
9.13 Anti-cancer Activity
In looking at multiple studies, Powolny and Singh (2008) [47].
conclude that organosulfur compounds such as DAS, DADS,
and DATS act by arresting the cell cycle of cancerous cells.
Data from Miroddi et al. (2011) [28] and Omar and Al-Wabel
(2009) [44] support this, showing that these allyl derivatives act
as antioxidants and arrest the cell cycle. DATS was found to
have the most significant role by Powolny and Singh (2008)
[47], and is even a potential skin cancer fighting compound
(Wang et al. 2010) [62]. More specifically, garlic acts as anti-
inflammatory agent by altering cytokines and inhibiting NF-
kB activity in surrounding tissues (Keiss et al. 2003) [30]. In a
study using human promyeloleukemic cells, Dirsch et al.
(1998) [17] found that ajoene prompted apoptosis in cancerous
cells but not healthy ones; this might be due to peroxide
production. In an in-vitro study involving rats, Jastrzebsk et al.
(2007) [25] found that raw garlic had the strongest antioxidant
activity. Tsai et al. (2011) [59]. reviewed animal and cell studies
and found an inverse correlation between consumption of
garlic and presence of cancerous cells, suggesting it has
anticancer effects.
9.14 Cardio protective activity
Garlic is a popular supplement well-perceived as a healthy
choice among people looking to increase cardiovascular
wellness. Approximately 4% of all cardiovascular disease
patients and 30% of cardiovascular patients who use herbal
supplements take garlic (Yeh et al. 2006) [64]. Known risk
factors for cardiovascular disease include inflammation, high
cholesterol, high homocysteine, high blood pressure, diabetes
and dementia, including its most common form, Alzheimer's
disease. Indeed, as early as the 1920’s and 1930’s (Schlesinger
1926) [52], numerous studies do bear the beneficial
cardiovascular effects. Garlic is well reported to scavenge
oxidants, increase superoxide dismutase, catalase, glutathione
peroxidase, and glutathione levels, as well as inhibit lipid
peroxidation and inflammatory prostaglandins. Garlic also
reduces cholesterol synthesis by inhibiting 3-hydroxy-3-
methylglutaryl-CoA. Garlic has been shown to inhibit LDL
oxidation, platelet aggregation, arterial plaque formation,
decrease homocysteine, lower blood pressure, and increase
microcirculation, which is important in diabetes, where
microvascular changes increase heart disease and dementia
risks. Garlic may also help prevent cognitive decline by
protecting neurons from neurotoxicity and apoptosis, thereby
preventing ischemia- or reperfusion-related neuronal death and
by improving learning and memory retention (Borek 2006) [7].
Garlic may also possess anti-inflammatory abilities to suppress
the nuclear factor-kappa B activation pathway (Aggarwal et al.
2004) [1].
9.15 Alzheimer’ Disease Protective activity
Known for its neuroprotective abilities in vitro (Peng et al.
2002) [45], aged garlic has been looked to for multiple benefits
that some researchers believe may address a number of
underlying mechanisms which contribute to the classic
Alzheimer beta-amyloid plaque. According to one author,
garlic: “is expected to produce cumulative benefits and exhibit
enhanced neuroprotection by virtue of being “natural statin”,
“natural NSAID”, “natural anti-oxidant”, “natural anti-
apoptotic agent” and “memory enhancer”, a combination of
many single-ingredient synthetic pharmaceutical drugs
currently used for Alzheimer's therapy, only with least adverse
effects (Chauhan 2006) [15].” Unfortunately, there is a dearth of
clinical studies showing of aged garlic extract in relation to
Alzheimer's pathology, except for reports showing improved
behavior in senescence accelerated mice after garlic treatment
(Nishiyama et al. 2001) [42]. Given the multiple-mechanistic
possibilities and minimal risk associated with its use, garlic
seems a prudent recommendation for prevention and
treatment. Since aged garlic is best studied in relation to
Alzheimer’s it may be the best form to employ.
10. Dosage
A commercial garlic product should provide a daily dose equal
to at least 4000mg (one to two cloves) of fresh garlic. The
cloves may be diced and mixed with wildflower honey for
palatability. This dosage translates to at least 10mg alliin or a
total allicin potential of 4000ug (Murray et al. 2006) [34]. In
dried form this would be 300-mg of garlic powder tablet
(standardized to 1.3 percent alliin or 0.6 percent allicin yield)
two to three times per day, or 7.2 g of aged garlic extract per
day (Tattelman 2005) [58]. In tincture form from fresh bulb as a
1:2 in 95 % alcohol, the dosage can be 40 drops up to six times
per day.
11. Adverse effects of garlic
The main adverse effect commonly associated with garlic
intake is breath odor, especially when raw forms of the herb
are used. Nausea and vomiting are other major adverse effects
and care should be taken in consuming high quantities.
Journal of Medicinal Plants Studies
Although an entire bulb produces little juice, it is potent and
can act as a strong emetic, even in small quantities. Although
garlic generally poses little in terms of safety issues, there are
isolated cases of topical garlic burns (Friedman et al., 2006) [20]
and anaphylaxis (Yin and Li, 2007) [63]. Rare garlic allergy has
been attributed to the protein allinase, which has induced
immunoglobulin E (IgE) mediated hypersensitivity responses
from skin prick testing (Kao et al., 2004) [28]. As a result, the
literature has generally cautioned against using garlic while
using anticoagulant therapy. There is a reported case of
spontaneous spinal or epidural hematoma in an 87 years old
man, with associated platelet dysfunction related to excessive
garlic ingestion (Saw et al., 2006) [51].
12. Conclusion
Garlic, from crushed to capsules, is consumed throughout the
world. This abstract demonstrates by documented studies the
benefits of garlic for its anti-microbial, antioxidant and anti-
inflammatory potential. It has been used to treat cardiovascular
diseases, including atherosclerosis, strokes, hypertension,
thrombosis and hyperlipidemias, as well as uses in
Alzheimer’s, diabetes, and cancer. Most impressive and
unique are its use and safety in children. Although some
studies show medicinal benefits of garlic, there are others
which do not. Clearly more studies are needed. Fresh and
powdered garlic are popular for food seasoning and should
continue to be used. Today, with the ever-growing resistant
organisms, garlic, taken alone or with other herbal antibiotics
such as grape seed extract or ginger, remains a powerful
antimicrobial agent. Clearly more studies are needed to refine
the use and improve the efficacy of this important plant
13. Acknowledgements
The authors acknowledge the inspired provided by Dr. Alhaj
Hakim Md. Yousuf Harun Bhuyan, honorable chief
mutaowalli & managing director, Hamdard Laboratories
(Waqf) Bangladesh, Kazi Mansur-Ul-Huq, Honorable
Chairman, Managing Committee, Hakim Said Eastern Medical
College & Hospital and Director Information and Public
Relation - Hamdard Bangladesh and Lt. Colonel Mahbubul
Alam Chowdhury (Ret:), Honorable Director, Hamdard
Foundation Bangladesh, for the preparation of this manuscript.
14. Conflicts of Interest
The authors declare no conflict of interests.
15. References
1. Aggarwal BB, Shishodia S. Suppression of the nuclear
factor-kappaB activation pathway by spice-derived
phytochemicals: reasoning for seasoning. Ann N Y Acad
Sci. 2004; 1030:434-41.
2. Alnaqeeb MA, Ali M, Thomson M, Khater SH, Gomes
SA, Al-Hassan JM. Histopathological Evidence of
Protective Action of Garlic against Collagen and
Arachidonic Acid Toxicity in Rabbits. Prostagl. Leukot.
Essent. Fatty Acids. 1992; 46:301-306.
3. Amagase H. Clarifying the real bioactive constituents of
garlic. Journal of Nutrition. 2006; 136:716S-725S.
4. Ankri S, Miron T, Rabinkov A, Wilchek M, Mirelman D.
Allicin from garlic strongly inhibits cysteine proteinases
and cytopathic effects of Entamoeba histolytica.
Antimicrob. Agents Chemother. 1997; 10:2286-2288.
5. Ali M. Mechanism by which garlic (Allium sativum)
inhibits cyclooxygenase activity. Effect of raw versus
boiled garlic extract on the synthesis of prostanoids.
Prostaglandins Leukot Essent Fatty Acids. 1995;
6. Bangladesh National Unani Formulary, 2010.
7. Borek C. Garlic reduces dementia and heart-disease risk. J
Nutr. 2006; 136(3Suppl):810S-812S.
8. Block E. The Chemistry of Garlic and Onions. Sci. Amer.
1985; 252:114-119.
9. Bolton S, Null G, Troetel WH, The Medicinal Uses of
Garlic - Fact or Fiction. Am. Pharmacy. 1982; 22:448-
10. Bordia A, Verma SK, Vyas AK, Khabya BL, Rathore AS,
Bhu N, Bedi HK. Effect of Essential Oil of Onion and
Garlic on Experimental Atherosclerosis in Rabbits.
Atherosclerosis. 1977; 26:379-386.
11. Block E. Garlic and Other Alliums: The Lore and the
Science. Cambridge, UK: The Royal Society of
Chemistry, 2010.
12. Banerjee SK, Maulik SK. Effect of garlic on
cardiovascular disorders: a review. Nutr J. 2002; 1:4-18.
13. Borek C. Antioxidant health effects of aged garlic extract.
J Nutr. 2001; 131(3s):1010S-5S.
14. Borek C. Recent Advances on the Nutritional Benefits
Accompanying the Use of Garlic as a Supplement. New
port Beach, 1998, 15-17.
15. Chauhan NB. Effect of aged garlic extract on APP
processing and tau phosphorylation in Alzheimer's
transgenic model Tg2576.J Ethnopharmacol. 2006;
16. Caporaso N, Smith SM Ing RHK. Antifungal activity in
human urine and serum after injestion of garlic.
Antimicrobial Agents Chemother. 1983; 23:700-2.
17. Dirsch VM, Gerbes AL, Vollmar AM. Ajoene, a
compound of garlic, induces apoptosis in human
promyeloleukemic cells, accompanied by generation of
reactive oxygen species and activation of nuclear factor
kappa B. Mol Pharmacol. 1998; 53:402-407.
18. Essman E. J. The Medicinal Uses of Herbs. Filoterapia.
1984; 55:279-289.
19. Eidi A, Eidi M, Esmaeili E. Antidiabetic effect of garlic
(Allium sativum L.) in normal and streptozotocin-induced
diabetic rats. Phytomedicine. 2006; 13(9-10):624-9.
20. Friedman T, Shalom A, Westreich M. Self-inflicted garlic
burns: our experience and literature review. Int. J.
Dermatol. 2006; 45(10):1161-1163.
21. Farbman KS, Barnett ED, Bolduc GR, Klein JO.
Antibacterial Activity of Garlic and Onions; A Historical
Perspective. Pediatr. Infect. Dis. J. 1993; 12:613-614.
22. Freeman F, Kodera Y. Garlic chemistry: stability of S-(2-
propenyl) 2-propene-1sulfinothioate (allicin) in blood,
solvents, and simulated physiological fluids. J. Agric.
Food Chem. 1995; 43:2332-2338
23. Harunobu A, Petesch BL, Matsuura H, Kasuga S, Itakura
Y. Intake of garlic and its bioactive components. J. Nutr.
2001; 131:955S-962S.
24. Jalali FSS, Tajik H, Javedi S, Mohammadi BH, Athari
SSA et al. The efficacy of alcoholic extract of garlic on
the healing process of experimental burn wound in the
rabbit. Journal of animal and veterinary advances. 2009;
25. Jastrzebski Z, Leontowicz H, Leontowicz M, Namiesnik
J, Zachwieja Z, Barton H. et al. The bioactivity of
processed garlic (Allium sativum L.) as shown in vitro and
in vivo studies on rats. Food Chem Toxicol. 2007;
Journal of Medicinal Plants Studies
26. Jung YM, Lee SH, Lee DS, You MJ, Chung IK, Cheon
WH. et al. Fermented garlic protects diabetic, obese mice
when fed a high-fat diet by anti-oxidant effects. Nutr Res.
2000; (5):387-96.
27. Josling P. Preventing the common cold with a garlic
supplement: a double-blind, placebo-controlled survey.
Adv. Ther. 2001; 18:189-193.
28. Kao SH, Hsu CH, Su SN, Hor WT, Chang WH, Chow LP.
Identification and immunologic characterization of an
allergen, alliin-lyase, from garlic (Allium sativum). J.
Allergy Clin. Immunol. 2004; 113(1):161-168.
29. Kodera Y, Suzuki A, Imada O, Kasuga S, Sumioka I,
Kanezawa A. et al. Physical, chemical, and biological
properties of S-allylcystein, an amino acid derived from
garlic J. Agric. Food Chem. 2002; 50:622-632.
30. Keiss HP, Dirsch VM, Hartung T, Haffner T, Trueman L,
Auger J. et al. Garlic (Allium sativum L.) modulates
cytokine expression in lipopolysaccharide-activated
human blood thereby inhibiting NF-kB acivity. J. Nutr.
2003; 133:2171-2175.
31. Kalyesa R. Screening of indigenous plants for
antihelminthic action against human Ascaris lumbricoides.
Indian J. Physiol. Pharmacol. 1975; 19:47-49.
32. Ledezma E, Apitz-Castro R. Ajoene the main active
component of garlic (Allium sativum): a new antifungal
agent. Rev Iberoam Micol. 2006; 23:75-80.
33. Lemar KM, Miguel AA, Sonia C, Brian O, Carsten TM,
David L. Diallyl disulphide depletes glutathione in
Candida albicans: oxidative stress mediated cell death
studied by two-photon microscopy. Yeast. 2007;
34. Murray M, Pizzorno J. (Murray and Pizzorno eds.) Allium
sativum (Garlic) in The Textbook of Natural Medicine,
Churchill Livingstone Elsevier, St. Louis, 3rd edition,
2006, 734
35. Milner J. A. Garlic: its Anticarcinogenic and Antitumor
Properties. Nutr. Rev. 1996; 54:S82-86.
36. Makheja AN, Bailey JM. Antiplatelet Constituents of
Garlic and Onion. Agents Actions, 1990; 29:360-364.
37. Moyers S. Garlic in Health, History and World Cuisine;
Suncoast Press: St. Petersburg, FL, 1996, 1-36.
38. Miroddi M, Calapai F, Calapai G. Potential benefidical
effects of garlic in oncohematology. Mini Rev Med Chem.
2011; 11:461-472.
39. Mukherjee S, Lekil I, Goswami S, Das, DK. Freshly
Crushed Garlic is a Superior Cardioprotective Agent than
Processed Garlic. J. Agric. Food Chem. 2009; 57:7137-
40. Mikaili P, Maadirad S, Moloudizargari M,
Aghajanshakeri Sh, Sarahroodi S. Therapeutic Uses and
Pharmacological Properties of Garlic, Shallot, and Their
Biologically Active Compounds. Iran J Basic Med Sci.
2013; 16:1031-1048.
41. Mirelman D, Motsheit D, Vaton S. Inhibition of growth of
Entamoeba histolytica by Allicin, the active principle of
garlic extracts (Allium sativum). J. Infect. Dis. 1987;
42. Nishiyama N, Moriguchi T, Morihara N, Saito H.
Ameliorative effect of Sallylcysteine, a major thioallyl
constituent in aged garlic extract, on learning deficits in
senescence-accelerated mice, Journal of Nutrition, 2001;
43. Oshiba S, Sawai H, Tamada T. Inhibitory effect of orally
administered inclusion complex of garlic oil on platelet
aggregation in man. Igaku no Ayuma. 1990; 155(3):199-
44. Omar SH, Al-Wabel NA. Organosulfur compounds and
possible mechanism of garlic in cancer. Saud Pharm J.
2009; 18:51-58.
45. Peng Q, Buz'Zard AR, Lau BH. Neuroprotective effect of
garlic compounds in amyloid-beta peptide-induced
apoptosis in vitro. Med Sci Monit. 2002; 8(8):BR328-37.
46. Pendbhaje IS, Amit P, Shahin M, Pathan S, Raotole A,
Pattewar SV. Ethnopharmacoloy, Pharmacogosy and
Phytochemical Profile of Allium Sativum L. A Review.
Pharmacology online. 2000; 2:845-85.
47. Powolny AA, Singh SV. Multitargeted prevention and
therapy of cancer by diallyl trisulfide and related Allium
vegetable-derived organosulfur compounds. Cancer Lett.
2008; 229:305-314.
48. Rivlin RS. Historical Perspective on the Use of Garlic. J.
Nutr. 2001; 131:951S-954S.
49. Rahman K. Garlic and aging: new insights into an old
remedy. Ageing Res Rev. 2002; 2:39-56.
50. Reuter HD, Koch HP, Lawson LD. Therapeutic effects
and applications of garlic and its preparations. Pages 135-
213 in H. P. Koch and L. D. Lawson, editors. Garlic: the
science and therapeutic application of Allium sativum L.
and related species. Williams and Wilkins, Baltimore,
Maryland, USA, 1996.
51. Saw JT, Bahari MB, Ang HH, Lim YH. Potential drug-
herb interaction with antiplatelet/anticoagulant drugs.
Complement Ther. Clin. Pract. 2006; 12(4):236-241.
52. Sclesinger K. Knoublauch (allium sativum) als Helimittel
bei Artiosklerose Wein Ned Wochenschr. 1926; 76:1076-
53. Sato T, Miyata G. The Nutraceutical Benefit, Part IV:
Garlic. Nutrition, 2000; 16:787-788.
54. Sainani GS, Desai DB, Natu MN, Katrodia KM, Valame,
VP, Sainani PG. Onion, Garlic and Experimental
Atherosclerosis. Jap. Heart J. 1979: 20:351-557.
55. Silagy CA, Neil HA. A meta-analysis of the effect of
garlic on blood pressure. J Hypertens. 1994; 12:463-8.
56. Shams-Ghahfarokhi M, Shokoohamiri MR, Amirrajab N,
Moghadasi B, Ghajari A, Zeini F. et al. In vitro antifungal
activities of Allium cepa, Allium sativum and ketoconazole
against some pathogenic yeasts and dermatophytes.
Fitoterapia. 2006; 77(4):321-323.
57. Sabitha P, Adhikari PM, Shenoy SM. Efficacy of garlic
paste in oral candidiasis. Trop Doct. 2005; 35(2):99-100.
58. Tattelman E. Health effects of garlic. Am Fam Physician.
2005; 72(1):103-6.
59. Tsai CW, Chen HW, Sheen LY, Lii CK. Garlic: health
benefits and actions. Bio Medicine, 2011. doi:
60. Touloupakis E, Ghanotakis DF. Nutraceutical use of
garlic sulfur-containing compounds. Adv Exp Med Biol.
2011; 698:110-121.
61. Tsai Y, Cole LL, Davis LE, Lockwood SJ, Simmons V,
Wild GC. Antiviral properties of garlic: in vitro effects on
influenza B, herpes simplex and coxsackie viruses. Planta
Med. 1985; 8:460-461.
62. Wang H, Yang J, Hsieh S, Sheen L. Allyl sulfides inhibit
cell growth of skin cancer cells through induction of DNA
damage mediated G2/M arrest and apoptosis. J. Agric.
Food Chem. 2010; 58:7096-7103.
63. Yin J Li H. Anaphylaxis Caused by Younger Garlic. J.
Allergy Clin. Immunol. 2007; 119(1):34.
64. Yeh YY, Yeh SM. Homocysteine-lowering action is
another potential cardiovascular protective factor of aged
Journal of Medicinal Plants Studies
garlic extract. Yeh YY. 2006; 136(3 Suppl):745S-749S.
65. Zhang XH, Lowe D, Giles P, Fell S, Connock MJ, Maslin
DJ. Gender may affect the action of garlic oil on plasma
cholesterol and glucose levels of normal subjects. J Nutr.
2001; 131:1471-1478.
... It has a tall, erect flowering stem that grows up to 1 m. it has four to twelve long, sword-shaped leaves attached to an underground stem. The bulb is rounded composed of up to about 15 smaller bulblets known as cloves (Alam et al., 2016;Kovarovič et al., 2019;Morales-González et al., 2019). It possesses high nutritive value. ...
... epilepsy, cataract and colic pain(Alam et al., 2016;Morales-González et al., 2019). Its pharmacologically active constituents display broad-spectrum activities including antibacterial, antiviral, antifungal, antiprotozoal,antipyretic, antioxidant, anticoagulant, anticarcinogenic, antiasthmatic, analgesic, anti-inflammatory, and anticancer effects (El-Saber Batiha et al., 2020; Hussein et al., 2017; Morales-González et al., 2019). ...
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The incidence of hypertension is very high in human societies and its treatment is the most important priority in many countries. Knowledge of the plants that are used may provide insight on their properties, for further exploration. This study aimed to collect the knowledge on traditional medicine for the treatment of hypertension in different regions of Morocco. We reviewed 145 research publications based on data from the six explored regions of Morocco published until August 2021 in various journals. This was achieved using literature databases: Google, Google Scholar, PubMed, Medline, Science Direct and Researchgate. The findings of this study indicated that 23 plants have been reported to possess antihypertensive activities in in vivo / in vitro experiments, while 81 plants had not been studied for such an activity. Plants from the Lamiaceae, Asteraceae and Apiaceae families were used most often. Leaves were the plant parts used most often. Decoction was the main preparation method. Twenty three plants have been explored experimentally for their antihypertensive activity. This review provides baseline data for plant species used to treat hypertension in Morocco and provides new areas of research on the antihypertensive effect of these plants.
... Every sickness has a remedy in the plant kingdom (Mintah et al. 2019). A. sativum (Garlic) plant is a member of the Liliaceae family and has antibacterial, antifungal, antiviral, anthelmintic, immune-stimulating, hepatoprotective, antioxidant and anticancer activities (Alam et al. 2016). As a bulb, garlic has many cloves that are grouped together and enclosed in whitish layers (Neeraj et al. 2014). ...
... S. nigra extract blocks the specific viral glycoproteins of the influenza virus and thus disrupts the process of attachment of the virus to the cell [50]. The extract of A. hippocastani [51] has also shown an effect on extracellular virions, and numerous experiments have been performed with A. sativum extracts to demonstrate its virucidal activity, as well as its inhibition of the attachment and entry of human, animal, and plant viruses into host cells [52][53][54][55].The plant inhibitory effects on the viral absorption, especially for H. perforatum and G. glabra, possess a correlation with the antibacterial inhibitory activity, which demonstrated their successful antiviral and antibacterial applications. ...
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Background: Bulgaria is a country with a wide range of medicinal plants, with uses in traditional medicine dating back for centuries. Methods: Disc diffusion assay was used to evaluate the antimicrobial activity of the plant extracts. A cytopathic effect inhibition test was used for the assessment of the antiviral activity of the extracts. The virucidal activity of the extracts, their influence on the stage of viral adsorption, and their protective effect on uninfected cells were reported using the end-point dilution method, and Δlgs was determined as compared to the untreated controls. Results: The results of the study reveal that the antibacterial potential of G. glabra and H. perforatum extracts in Gram-positive bacteria is more effective than in Gram-negative bacteria. When applied during the replication of HSV-1 and HCov-OC-43, only some of the extracts showed weak activity, with SI between 2 to 8.5. Almost all tested extracts inhibited the extracellular virions of the studied enveloped viruses (HSV-1 and HCov-OC-43) to a greater extent than of the non-enveloped viruses (PV-1 and HAdV-5). They inhibited the stage of viral adsorption (HSV-1) in the host cell (MDBK) to varying degrees and showed a protective effect on healthy cells (MDBK) before they were subjected to viral invasion (HSV-1). Conclusion: The antipathogenic potential of extracts of H. perforatum and G. glabra suggests their effectiveness as antimicrobial agents. All 13 extracts of the Bulgarian medicinal plants studied can be used to reduce viral yield in a wide range of viral infections.
... It has minerals, vitamins, and other useful components to improve the health of humans [6]. Garlic can be used to cure many diseases like high blood pressure, cholesterol [7], cancer, wound healing [8], cold, cough, asthma, arthritis [9], backache, bronchitis, antioxidant chronic fever [10], tuberculosis, malaria, rhinitis, obstinate skin disease, discolouration of the skin, leprosy, leucoderma, itches [7], piles, fracture of bone, fistula, gout, urinary diseases, diabetes, kidney stone, anaemia [6], cataract and night blindness [10]. Garlic has the potential of an antioxidant. ...
Butter is a popular dairy product composed of mainly milk fat and other minor components such as water, vitamins, enzymes, and minerals which are beneficial for health. The study was to investigate the effect of garlic (Allium sativum) powder addition on the chemical, physical, microbial, and sensory properties of butter, incorporated with garlic at the rate of 2% garlic chips and 2% garlic powder (w/w) and 2% garlic chips and 4% garlic powder (w/w). Butter samples were analyzed for physical, chemical, microbial and sensory properties during refrigerated storage at 7 °C. The physico-chemical and sensory characteristics were analyzed during storage. Moisture, total solids, fat, free fatty acids, titratable acidity, and pH were significantly different (p<0.05) among the treatments on day one. The results of this study revealed that the moisture (14.09±0.10%) and total solids (85.91±0.10%) content were significantly (p<0.05) higher in butter without incorporating garlic chips and powder. Fat content (80±0.00%) was significantly (p<0.05) lowest in butter incorporated with 2% garlic chips and 4% garlic powder. While free fatty acids were significantly (p<0.05) highest in butter incorporated with 2% garlic chips and 4% garlic powder and lowest in butter without added garlic chips and powder. pH (6.09±0.03%) was significantly (p<0.05) lowest in butter incorporated with 2% garlic chips and 4% garlic powder. Titratable acidity (0.13±0.02%) was significantly (p<0.05) lower in butter without added garlic chips and powder 2% garlic chips and 4% garlic powder added to butter showed the highest (48.75±1.18 Mm/g) antioxidant activity. During storage, the pH value was significantly (p<0.05) decreased and titratable acidity was increasing with the storage period. The cohesiveness, gumminess and springiness were significantly (p<0.05) increased. Organoleptic characteristics revealed that 2% garlic chips and 4% garlic powder added to butter had the highest mean score of overall quality of all sensorial properties. Finally, most of the panelist accepted, that butter is made from 2% garlic chips and 4% garlic powder than other types of butter.
... Maize plants exposed to the salinity stress and exogenous application proline alleviated NaCl toxic effect by improving yield parameters [36]. [37] investigated that the proline foliar application on germination of seeds, growth of seedlings and potassium and sodium ratio. Exogenous application of proline alleviates the toxic ions and maintained other essential nutrients in O. europaea both young and old leaves [38]. ...
Plant growth and development processes are affected under environmental/abiotic stresses. Salinity limits rooting system regarding uptake of essential nutrient that is required for plant growth and development. A field experiment was conducted at the Botanical Garden University of Balochistan Quetta to explore the role of Plant Growth Regulators (PGRs) i.e., Proline (PRO; 1 µM), Moringa leaf extract (MLE; 10%), Thiourea (TU; 10 mM) and Salicylic acid (SA; 250 µM) on nutritional status of Plantago ovata under (120 mM) salinity stress (SS). After 10 days of germination seedlings were subjected to salinity stress (in comparison to control field plants) and after 1 week of salinity stress plants were foliarly supplemented with optimized levels of PGRs. Moreover, later one week of foliar spray plants was harvested and analyzed for nutrient status. Data revealed statistically significant results except for shoot calcium (P< 0.05). Salinity hampered the essential nutrients (Magnesium, Calcium, Potassium and Nitrate); however, the contents of Na + enhanced in both Root and Shoot under salinity stress. However, foliar application of PGRs improved the essential nutrients of Plantago ovata (Mg +2 , Ca +2 , K + and NO 3-) except in the case of Na + that is decreased under foliar supplementation thus defining the positive role of PGRs in ameliorating the toxic impacts of salinity. It is concluded that exogenous application of PGRs recommended for improved nutritional status of plants under stress condition with MLE and TU to be the most effective treatment under salinity stress. Shaukat et al. 49
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Introduction: This study aims at the biological profiling of Allium sativum , Zingiber officinale , Nigella sativa , Curcuma longa , Mentha piperita , Withania somnifera , Azadirachta indica , and Lawsonia inermis as alternatives against onychomycosis to combat the treatment challenges. Methods: An extract library of aqueous (DW), ethyl acetate (EA), and methanol (M) extracts was subjected to phytochemical and antioxidant colorimetric assays to gauge the ameliorating role of extracts against oxidative stress. RP-HPLC quantified therapeutically significant polyphenols. Antifungal potential (disc diffusion and broth dilution) against filamentous (dermatophytes and non-dermatophytes) and non-filamentous fungi ( yeasts ; Candida albicans ), synergistic interactions (checkerboard method) with terbinafine and amphotericin-B against resistant clinical isolates of dermatophytes ( Trichophyton rubrum and Trichophyton tonsurans ) and non-dermatophytes ( Aspergillus spp., Fusarium dimerum , and Rhizopus arrhizus ) , time-kill kinetics, and protein estimation (Bradford method) were performed to evaluate the potential of extracts against onychomycosis. Results: The highest total phenolic and flavonoid content along with noteworthy antioxidant capacity, reducing power, and a substantial radical scavenging activity was recorded for the extracts of Z. officinale . Significant polyphenolics quantified by RP-HPLC included rutin (35.71 ± 0.23 µg/mgE), gallic acid (50.17 ± 0.22 µg/mgE), catechin (93.04 ± 0.43 µg/mgE), syringic acid (55.63 ± 0.35 µg/mgE), emodin (246.32 ± 0.44 µg/mgE), luteolin (78.43 ± 0.18 µg/mgE), myricetin (29.44 ± 0.13 µg/mgE), and quercetin (97.45 ± 0.22 µg/mgE). Extracts presented prominent antifungal activity against dermatophytes and non-dermatophytes (MIC-31.25 μg/ml). The checkerboard method showed synergism with 4- and 8-fold reductions in the MICs of A. sativum , Z. officinale , M. piperita , L. inermis , and C. longa extracts and doses of amphotericin-B (Amp-B) and terbinafine (against non-dermatophytes and dermatophytes, respectively). Furthermore, the synergistic therapy showed a time-dependent decrease in fungal growth even after 9 and 12 h of treatment. The inhibition of fungal proteins was also observed to be higher with the treatment of synergistic combinations than with the extracts alone, along with the cell membrane damage caused by terbinafine and amp-B, thus making the resistant fungi incapable of subsisting. Conclusion: The extracts of A. sativum , Z. officinale , M. piperita , L. inermis , and C. longa have proven to be promising alternatives to combat oxidative stress, resistance, and other treatment challenges of onychomycosis.
Background Unhealthy dietary patterns and lifestyle modification could exacerbate heart-related diseases and the risk factors for CVD mortality includes dyslipidemia, hypertension, and diabetes mellitus. However, controlling cholesterol, triglycerides, and low-density lipoprotein could minimize the occurrence of risk factors. A homeopathic preparation of Allium sativum is effective for the treatment of various ailments, however, the usage of the drug by homeopathic practitioners for therapeutic purposes must be validated scientifically to ascertain its action. Objectives The present study is designed to evaluate the hypolipidemic and anti-inflammatory activities of the homeopathic drug A. sativum 6C in mitigating dyslipidemic changes in different types of dyslipidemic rat models. Methods Assessment of serum biochemical parameters, evaluation of liver cytokines, and immunoblot analysis of inflammatory proteins were performed in this study. Results Administration of a homeopathic preparation of A. sativum 6C to different types of dyslipidemic rat model showed significant improvement in the serum lipid profile, reduction in the activity of serum marker enzymes (AST, ALT, LDH, and ALP), and also, decrement in the levels of liver inflammatory cytokines (TNFα, IL-6, and IL-1β). Further, the expression of inflammatory proteins (CRP, P-Selectin, E- Selectin) was downregulated in the liver of dyslipidemic animals upon administration of A. sativum6C. However, among the various dyslipidemic models, the efficacy of A. sativum is more profound in the HCD and HFD-fed animals. On analyzing the GC-MS data of A. sativum6C, it is shown to contain some active ingredients (Undecane; 1-methylene 1H-Indene, and Oxazolam) that might be responsible for the anti-inflammatory properties of A. sativum 6C. Conclusion Homeopathic preparation of A. sativum 6Cmodulatesthe levels of serum biochemical parameters and inflammatory events in HCD-T and T2DM-T animals more than in the STZ-T animals, thus it mitigates metabolic dysfunction. Therefore, this study brings limelight to support the potential of the homeopathic drug, A. sativum for therapeutic purposes in correcting metabolic dysfunction associated with the present lifestyle.
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Himalayan communities illustrate a rich agriculture–medicine use system that not only provides adequate dietary diversity and nutrition but also delivers therapeutic security. This study explores the food–medicine interface as observed by the marginal hill communities in the central Himalaya with an aim to assess traditional agriculture and food plants with relation to dietary diversity and nutritional and medicinal values based on comprehensive research. A total of 445 respondents were interviewed to obtain data on food intakes using dietary recall methods and dietary diversity indices (DDIs). The ethnomedical use of plant species was gathered from respondents as well as from various published studies for respective species. Nutritional parameters were collected from the Indian Food Composition Table developed by the ICMR, India to analyze the average nutritional intake. The traditional food system achieves the dietary and nutritional needs of the community within the standard norms. The average household dietary diversity of 7.45, 7.34, and 8.39 in summer, monsoon, and winter seasons, respectively, sustain 79, 74, and 93% of energy requirements in respective, seasons. The average food consumption score (FCS) was 73.46, and all the food exhibited rich phytochemicals, such as amino acids, alkaloids, carotenoids, flavonoids, glycosides, and phenolic acids. These plants also provided effective treatments against several ailments and illnesses, such as cardiovascular diseases, diabetics, gastrointestinal issues, and inflammation The indigenous cuisines also have significant food and medicinal values. Considering that the community had significant knowledge of food systems with their nutritional and therapeutic utility, there is a need to protect and document this indigenous knowledge. Also, most of the crops are still under cultivation, so there is a need to create more awareness about the nutritional and therapeutic value of the system so that it could be retained intact and continued. The implications of this research are of both academic importance and practical significance to ensure food–medicine security and avoid malnutrition among rural communities. It is expected that the study would lead to renewed thinking and policy attention on traditional agriculture for its role in food and nutritional security that may lead to a sustainable food supply system.
Ethnopharmacological relevance For thousands of years, garlic (Allium sativum Linnaeus) has been consumed in food and health by numerous civilizations. Cryptosporidium (C.) parvum is an apicomplexan parasite that causes a gastrointestinal disease, with the most common symptoms being watery diarrhea. Although several substances have been tried for its anti-cryptosporidial action, there is no effective treatment for Cryptosporidium disease, especially in immunocompromised individuals. The present study aimed firstly to characterize the bio-active compounds in Allium sativum L. and secondly to evaluate its efficacy as a therapy for cryptosporidiosis especially in immunocompromised mice. Materials and methods This was accomplished by evaluating the parasitological and histopathological parameters in the experimentally infected immunocompetent and immunocompromised mice. Also, the cytokine profile during the experimental time was recorded through the measuring of T helper (h)1, Th2 and Th17 cells cytokines. Immunosuppressed mice were given 0.25 μg/g per day of dexamethasone orally, before infection with Cryptosporidium parvum oocysts, for fourteen consecutive days. Starting 10 days post infection (PI), nitazoxanide (100 mg/kg per day) or Allium sativum (50 mg/kg per day) was given orally for fourteen consecutive days. Results Our results showed that oocyst shedding, on the 32nd day PI, in immunocompromised infected group treated with Allium sativum (354.11, 99.35% PR) showed a significant decrease when compared to its corresponding group treated with nitazoxanide (4369.14, 92.05% PR). On the 32nd day PI, all cytokines levels have been decreased to levels that were similar to those of their uninfected corresponding control groups; also, the histopathological changes and the loss in animals’ body weight had been improved. Treatment with nitazoxanide did not result in infection clearance or a reduction in the increased cytokines' levels. Conclusion Allium sativum L. displayed high efficacy as a potential therapeutic agent against Cryptosporidium, which supports its traditional usage in parasite diseases.
Several herbal formulations that are widely accepted as therapeutic agents in chronic diseases, such as diabetes, cardiovascular diseases, asthma, cancer, pulmonary diseases, etc., originated in India. The most common myth associated with the use of herbs is that these formulations are completely safe and could be consumed by patients on their own without any physician's prescription. However, when these herbs are concomitantly consumed with allopathic medicines, there are chances of herb–drug interactions (HDIs). The prime hitch associated with the HDI is its potential to alter the pharmacokinetic and pharmacodynamic (PK/PD) properties of the drug. The PK drug interactions are more common as compared to the PD drug interactions as it involves alterations in the absorption, distribution, metabolism, and excretion (ADME) of the drug by inhibiting or inducing drug-metabolizing enzymes, mainly cytochromes P450 (CYPs) and drug transporters (DTs), such as P-glycoprotein (P-gp). The latter is responsible for changing the plasma concentration level of the drug. The CYPs are a superfamily of enzymes containing hemoprotein monooxygenases that metabolize chemical compounds and detoxify drugs. CYP1A1/2, CYP2B6, CYP2C8/9/19, CYP2D6, CYP2E1, CYP3A4/5, and CYP4A are the main CYP isoforms involved in the biotransformation of a drug. Likewise, the DTs are important in the drug development process and also play a crucial role in the ADME of the drug. P-gp is an efflux transporter that limits the absorption of the drug in the intestine and focuses on the drug bioavailability. The following chapter is designed to highlight the CYPs and P-gp PK interactions between few commonly used herbs in India concomitantly administrated with different allopathic medicines. Moreover, the foremost aim of this chapter is to spread the knowledge among the readers about the inimical effects of the HDI.
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This investigation was evaluation of efficacy of alcoholic extract of garlic on the healing of burn wound in rabbit. In this study, 10 male white rabbits, weighing a mean of 2.200 +/- 240 g were used. Based on Hoekstra standard model, a rectangular burn wounds (2x2.5 cm(2)) were created on back region of each rabbit. The wounds in experimental group were treated with alcoholic extract of garlic (5 mL) every day for 21 days. In the control group, the wounds were washed with normal saline (5 mL) at same frequency and time of day. The wounds were evaluated form clinical (rate of wound contraction) and microbiological (total count) aspects. The clinical findings of the present study were showed wound surfaces in the experiment group were significantly decreased in comparison to the control wounds (p<0.05). Microbiological evaluation was showed that total bacterial count of wound surfaces in garlic-treated group was lesser than this variable in the control wounds (p<0.05). With attention to these results, alcoholic extract of garlic can be considered as a topical agent for accelerating and enhancement burn wound healing in rabbit.
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Objective(s): Garlic (Allium sativum L. family Liliaceae) is well known in Iran and its leaves, flowers, and cloves have been used in traditional medicine for a long time. Research in recent decades has shown widespread pharmacological effects of A. sativum and its organosulfur compounds especially Allicin. Studies carried out on the chemical composition of the plant show that the most important constituents of this plant are organosulfur compounds such as allicin, diallyl disulphide, S-allylcysteine, and diallyl trisulfide. Allicin represents one of the most studied among these naturally occurring compounds. In addition to A. sativum, these compounds are also present in A. hirtifolium (shallot) and have been used to treat various diseases. This article reviews the pharmacological effects and traditional uses of A. sativum, A. hirtifolium, and their active constituents to show whether or not they can be further used as potential natural sources for the development of novel drugs. Materials and Methods: For this purpose, the authors went through a vast number of sources and articles and all needed data was gathered. The findings were reviewed and classified on the basis of relevance to the topic and a summary of all effects were reported as tables. Conclusion: Garlic and shallots are safe and rich sources of biologically active compounds with low toxicity. Further studies are needed to confirm the safety and quality of the plants to be used by clinicians as therapeutic agents.
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Garlic (Allium sativum), a member of the family Liliaceae, contains an abundance of chemical compounds that have been shown to possess beneficial effects to protect against several diseases, including cancer. Evidence supports the protective effects of garlic in stomach, colorectal, breast cancer in humans. The protective effects appear to be related to the presence of organosulfur compounds, predominantly allyl derivatives, which also have been shown to inhibit carcinogenesis in forestomach, esophagus, colon, mammary gland and lung of experimental animals. The exact mechanisms of the cancer-preventive effects are not clear, although several hypotheses have been proposed. Organosulfur compounds modulate the activity of several metabolizing enzymes that activate (cytochrome P450s) or detoxify (glutathione S-transferases) carcinogens and inhibit the formation of DNA adducts in several target tissues. Antiproliferative activity has been described in several tumor cell lines, which is possibly mediated by induction of apoptosis and alterations of the cell cycle. Organosulfur compounds in garlic are thus possible cancer-preventive agents. Clinical trials will be required to define the effective dose that has no toxicity in humans.
Forty-two healthy male albino rabbits weighing around 1 Kg were divided into 4 groups. Group I (8)- fed on normal stock diet, Group II (8)- fed on stock diet plus cholesterol (0.5 gm in 5 ml of olive oil). Group III (15)- received stock diet plus cholesterol plus garlic (0.25 gm) juice. Group IV (11)- received stock diet plus cholesterol plus onion (2.5 gm) juice. The animals were closely observed and followed for 16 weeks. Approximately every 4 weeks, blood samples were collected for estimation of various parameters (S. cholesterol, S. triglycerides, S. lipoproteins, S. phospolipids, and fibrinolytic activity). At the end of experiment, animals were sacrificed and degree of aortic atherosclerosis was graded (grade 0 to 4) in different groups and compared. Experimental study revealed that both garlic and onion (garlic more than onion) had significant effect in inhibiting the rise in S. cholesterol, S. triglycerides, S. beta lipoproteins, and S. phospolipids and significant effect in enhancing the fibrinolytic activity. The beta: alpha ratio was altered favourably and the ratio was kept close to normal. As regards the degree of aortic atherosclerosis as seen on post mortem, it was significantly less in garlic and onion group when compared with pure cholesterol group.
Recent years have seen an increasing emphasis on foods and food components in disease prevention. Garlic (Allium sativum L.), one of the best-researched herbal remedies, holds a unique position in history, traditionally employed to treat infection, colds, diabetes, heart disease, and a host of other disorders. Clinically, it has been evaluated for lowering blood pressure, cholesterol, and glucose concentration, as well as for the prevention of arteriosclerosis and cancer. Epidemiologically, garlic consumption inversely correlates with the risk of oral, stomach, esophageal, colon, and prostate cancers. In addition, the biological activities of garlic, including antibacterial, antithrombotic, antioxidant, immunomodulatory, and antidiabetic actions and modulation of drug metabolism, have been extensively investigated. Here, we briefly summarize the recent findings on garlic and its sulfur-containing compounds in preventing cardiovascular diseases and cancer, along with its modulation of drug-metabolizing enzymes and membrane transporter activities. Finally, garlic safety and drug interaction are discussed.
S-(2-Propenyl) 2-propene-1-sulfinothioate (allicin), which is one of the constituents of freshly crushed garlic (garlic homogenate), was synthesized, and its stability in blood, ethyl acetate, methanol, simulated gastric fluid (SGF, pH 1.2), simulated intestinal fluid (STF, pH 7.5), and water (pH 1.2 and 7.5) and under simulated digestive conditions (sequential combination of SGF and SIF) was investigated by HPLC. Although neat allicin decomposes rapidly at 37 degrees C, it is more stable in protic polar methanol than in aprotic polar ethyl acetate. Approximately 90% of the allicin remained after incubation at 37 degrees C for 5 h in water at pH 1.2 and 7.5, Only traces of allicin could be detected after it was incubated in blood for 5 min. The allicin content and allicin-producing potential of commercial garlic preparations were also analyzed. The allicin contents in these garlic preparations were less than 1 ppm, and the allicin-producing potential was severely suppressed under simulated digestive conditions (sequential combination of SGF and SIE). The transformation products of allicin. [(E)-ajoene, 2-ethenyl-4H-1,3-dithiin, diallyl disulfide] were identified.