Zhouetal. Biotechnol Biofuels (2020) 13:88
Biosynthesis ofacetylacetone inspired byits
Yifei Zhou1,2, Yamei Ding3, Wenjie Gao1, Jichao Wang1, Xiutao Liu1,2, Mo Xian1, Xinjun Feng1*
and Guang Zhao1,4*
Background: Acetylacetone is a commercially bulk chemical with diverse applications. However, the traditional
manufacturing methods suﬀer from many drawbacks such as multiple steps, harsh conditions, low yield, and environ-
mental problems, which hamper further applications of petrochemical-based acetylacetone. Compared to conven-
tional chemical methods, biosynthetic methods possess advantages such as being eco-friendly, and having mild
conditions, high selectivity and low potential costs. It is urgent to develop biosynthetic route for acetylacetone to
avoid the present problems.
Results: The biosynthetic pathway of acetylacetone was constructed by reversing its biodegradation route, and
the acetylacetone was successfully produced by engineered Escherichia coli (E. coli) by overexpression of acetylac-
etone-cleaving enzyme (Dke1) from Acinetobacter johnsonii. Several promising amino acid residues were selected
for enzyme improvement based on sequence alignment and structure analysis, and the acetylacetone production
was improved by site-directed mutagenesis of Dke1. The double-mutant (K15Q/A60D) strain presented the highest
acetylacetone-producing capacity which is 3.6-fold higher than that of the wild-type protein. Finally, the strain accu-
mulated 556.3 ± 15.2 mg/L acetylacetone in fed-batch fermentation under anaerobic conditions.
Conclusions: This study presents the ﬁrst intuitive biosynthetic pathway for acetylacetone inspired by its biodegra-
dation, and shows the potential for large-scale production.
Keywords: Acetylacetone biosynthesis, Acetylacetone-cleaving enzyme, Rational design, Site-directed mutagenesis,
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Acetylacetone, also known as 2,4-pentanedione (CAS
No. 123-54-6), is an important commodity chemical and
widely used as a fuel additive, as dyeing intermediate, in
the ﬁelds of metal extraction, metal plating, and resin
modiﬁcation . Traditionally, acetylacetone is manufac-
tured through chemical routes using acetone and ketene,
which is produced by pyrolysis of acetone or acetic acid
at a temperature of 700–800°C, with carbon monoxide,
methane, hydrogen formed as by-products [2, 3]. In
speciﬁc, esteriﬁcation of ketene and acetone forms iso-
propenyl acetate (IPA), in the presence of a strong acid
catalyst. en, IPA is transformed into acetylacetone
at 500–600°C with metallic molybdenum as a catalyst,
whereby the yield is only about 45%. In conclusion, the
chemical routes suﬀer from drawbacks such as multiple
steps, harsh conditions, low yield, and environmental
problems, which hamper further applications of petro-
chemical-based acetylacetone. To address the issue, new
methods need to be developed for acetylacetone prepara-
tion. Compared to conventional chemical methods, bio-
synthetic methods are expected to have advantages such
as being eco-friendly and having mild conditions, high
Biotechnology for Biofuels
*Correspondence: firstname.lastname@example.org; email@example.com
1 CAS Key Laboratory of Biobased Materials, Qingdao Institute
of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences,
Qingdao 266101, China
Full list of author information is available at the end of the article
Page 2 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
selectivity and low potential costs , and have been used
to produce numerous products, e.g., bio-based chemicals
, pharmaceuticals , biopolymers . For acetylac-
etone, the theoretical yield is as high as 1.5mol/mol glu-
cose by bioconversion. Predictably, low cost will also be
obtained thanks to the cheap carbon source and the high
Limited studies indicate that acetylacetone is biode-
gradable . A strain of Acinetobacter johnsonii was
isolated and proved to have the ability to utilize acetylac-
etone as a sole carbon source. To reveal the decomposi-
tion mechanism, a novel C–C bond-cleaving enzyme,
acetylacetone-cleaving enzyme (Dke1, EC 126.96.36.199),
was found and puriﬁed from A. johnsonii . e Dke1
enzyme can activate oxygen to cleave acetylacetone into
acetate and methylglyoxal, followed by the conversion
of methylglyoxal into lactate by glyoxalase. However, the
natural biosynthesis of acetylacetone has not yet been
reported. In previous studies, some non-natural prod-
ucts had been bio-synthesized by reversing their biodeg-
radation pathway. An artiﬁcial biosynthetic pathway to
methylacetoin was constructed by redirecting the methy-
lacetoin biodegradation, and the titer achieved at 3.4g/L
by enzyme screening and metabolic engineering . e
direct biocatalytic route of 1,4-butanediol mirroring its
biodegradation pathway was reported with a produc-
tion of 18g/L . In addition, the engineered reversal of
the β-oxidation cycle was adopted for fatty-acid-derived
compounds’ biosynthesis, and a series of short-,
medium-, and long-chain products were obtained at high
yields [11–13]. ese successful cases provided a possible
strategy to our research for acetylacetone biosynthesis.
In this work, we established for the ﬁrst time the bio-
synthetic pathway of acetylacetone from fermentable
sugars (Fig.1) inspired by the known acetylacetone bio-
degradation pathway. It was proved that the acetylacetone
decomposition process catalyzed by Dke1 was revers-
ible. e Dke1 activity was improved by rational design,
resulting in enhanced acetylacetone production under
shake-ﬂask conditions, and the underlying mechanism
was proposed. Fed-batch fermentation was conducted to
evaluate the potential for large-scale production.
Design andverication oftheacetylacetone biosynthesis
Acetylacetone is not a naturally occurring metabolite;
however, it can be catabolized by Acinetobacter johnsonii
as a carbon source . As reported, one molecule of acet-
ylacetone degrades into one acetate and one methylgly-
oxal catalyzed by acetylacetone-cleaving enzyme Dke1.
Methylglyoxal can be produced marginally through gly-
colysis , and acetate is a major product during sugar
fermentation especially under anaerobic conditions.
us, we proposed to achieve acetylacetone production
from methylglyoxal and acetate by redirecting the reac-
tion. As shown in Fig. 1, glyceraldehyde 3-phosphate
from glycolysis pathway was converted into glycerone
phosphate and methylglyoxal by the action of triose-
phosphate isomerase (TpiA) and methylglyoxal synthase
(MgsA), respectively. Acetyl-CoA was converted into
acetate by acetate kinase. Methylglyoxal and acetate were
ﬁnally transformed into acetylacetone by Dke1.
To test the hypothetical acetylacetone biosynthetic
pathway, acetylacetone-cleaving enzyme (Dke1) from
A. johnsonii (accession No. Q8GNT2.1) was cloned into
plasmid pETDuet-1, and then the recombinant plasmid
was introduced into E. coli BL21 (DE3) to construct an
acetylacetone producing strain. e expression of the
Dke1 was ﬁrst checked by SDS-PAGE, and the corre-
sponding bands with molecular weights of 17kDa were
observed clearly (Additional ﬁle1: Fig. S1). e resulting
Fig. 1 Biodegradation-inspired biosynthetic pathway of acetylacetone. Acetylacetone biodegradation is presented with a dashed line, and the
constructed biosynthetic pathways are presented using a solid line. The enzymes used are as follows: AckA, acetate kinase; TpiA, triose-phosphate
isomerase; MgsA, methylglyoxal synthase; Dke1, acetylacetone-cleaving enzyme
Page 3 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
strain Q3030 was cultured under shake-ﬂask conditions,
and the fermentation products were analyzed by GC–
MS. However, no acetylacetone was detected. We then
referred back to the acetylacetone biodegradation path-
way. As the biodegradation of acetylacetone was acti-
vated by oxygen, we speculated the biosynthesis direction
was suppressed under aerobic conditions. us, anaero-
bic cultivation in a sealed serum bottle with nitrogen
was carried out for further veriﬁcation. is time, the
production of acetylacetone was conﬁrmed by GC–MS
analysis. As shown in Fig.2, a speciﬁc peak with a mass
of 100Da was detected, and dissociation of this ion led
to other masses such as 43 and 85Da, exactly the same
with acetylacetone standard. Under this condition, the
concentration of acetylacetone in fermentation broth was
32.6 ± 1.0mg/L. Furthermore, the production of acety-
lacetone was also performed in vitro in Tris buﬀer (pH
7.5) containing puriﬁed Dke1 protein, methylglyoxal and
ammonium acetate at 37°C, and 129.5 ± 8.9mg/L acety-
lacetone was accumulated in 24h. Compared with invivo
system, higher product titer was achieved with invitro
system, probably owing to a relatively simple pathway
without side reactions .
A suﬃcient supply of precursors is necessary for the
eﬃcient synthesis of the ﬁnal products . As one of
the main metabolites in E. coli under anaerobic condi-
tion, acetate should be suﬃcient for acetylacetone bio-
synthesis. But in general, methylglyoxal synthesis is
inhibited in cell [14, 17]. So the methylglyoxal content in
the ﬂask was improved by extra supplement with a con-
centration of 0.1mM or overexpression of the key genes
(tpiA and mgsA) for enhanced acetylacetone production
(Fig. 1). However, no more acetylacetone was accumu-
lated. Meanwhile, the invitro experiment with 20 mM
methylglyoxal was conducted, and the production of
acetylacetone (128.9 ± 8.6mg/L) did not show signiﬁcant
diﬀerence with that with 10mM methylglyoxal (p = 0.94).
So, we speculated that low activity of Dke1 might be the
primary reason of low acetylacetone yield. en, the acet-
ylacetone-cleaving enzyme was ﬁrst screened. Two more
genes encoding acetylacetone-cleaving enzyme from
Paraburkholderia xenovorans LB400 (P_Dke1, acces-
sion No. NC_007951.1) and Tistrella mobilis (T_Dke1,
accession No. NC_017966.1) were cloned and used for
acetylacetone production. Unfortunately, no acetylac-
etone was detected using engineered strains with P_Dke1
or T_Dke1. So, other strategies should be considered for
higher acetylacetone production.
Rational design ofDke1
Protein engineering using directed evolution or rational
design has been developed rapidly, and has acted as a
powerful tool for enzyme improvement [18, 19]. Espe-
cially, protein sequence information combined with
computational modeling tools can be used to iden-
tify promising amino acid sites and can be selected for
enzyme engineering . us, rational designstrategies
were considered for increasing Dke1 activity.
To improve the Dke1 catalytic activity, multiple amino
acid sequence alignment was performed between Dke1
and 27 other acetylacetone-cleaving enzymes or hypo-
thetical proteins sharing more than 60% identity with
Dke1 (Additional ﬁle 2: TableS1). Among 153 amino
acid residues in Dke1, 52 residues were conserved in all
28 proteins, and referred to as deﬁnitely conservedsites
(highlighted in red in Fig. 3); 44 amino acid residues
appeared in more than 14 other proteins, and deﬁned as
relatively conservedsites (highlighted in yellow in Fig.3);
Fig. 2 Veriﬁcation of the production of acetylacetone by engineered E. coli strain using GC–MS. GC chromatography (a) and mass spectrometry (b)
results are shown for acetylacetone standard (lower panel) and Q3030 culture (upper panel)
Page 4 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
and 19 amino acid residues rarely (less than 26%) showed
up in other proteins, and were called variable sites (high-
lighted in green in Fig. 3). Among the variation sites,
three amino acid residues were found conserved in all
proteins except Dke1, and site-directed mutagenesis was
performed to construct Dke1 single mutants harboring
each substitution as its homologs. en, shake ﬂask cul-
tivation was performed to test the eﬀect of each muta-
tion. e strain with Dke1 K15Q (Q3080) accumulated
60.6 ± 0.7mg/L acetylacetone, which is 1.9-fold higher
than that of the wild-type strain Q3030 (p < 0.01) (Fig. 4).
However, the strain with Dke1 Y21W (Q3082) only pro-
duced 28.8 ± 1.0mg/L acetylacetone, and the production
reduced dramatically to 7.0 ± 0.4mg/L when Dke1 S17D
was used in strain Q3081.
Meanwhile, 3D structure analysis of the wild-type
Dke1 was performed based on its crystal structure (PDB
ID: 3BAL). In the crystal structure of the ligand-free
Dke1, the Fe2+-coordinating His-62, His-64 and His-
104 comprise the substrate catalytic center . e
deﬁnitely conserved sites occupy about three quarters
of the substrate channel, which are the most important
amino acid residues for maintaining structure and func-
tion of the proteins (Additional ﬁle 1: Fig. S2). ese
sites would not be modiﬁed in this study. Most of the
relatively conservedsites cover about one quarter of the
substrate channel, while a few sites are located close to
the catalytic center. We analyzed the possible impact
from the mutation of the relatively conservedsites near
the catalytic center and all the variation sites. By analy-
sis of the key inﬂuence factors including properties of
amino acids, hydrogen bonding, electron distribution,
and charge properties, 5 sites were selected as potential
mutation sites for improving Dke1 activity (Table1). For
Fig. 3 Alignment of multiple amino acid sequences of Dke1 and other proposed acetylacetone-cleaving enzymes. The deﬁnitely conserved sites
are labeled in red, the relatively conserved sites are labeled in yellow, the variable sites are labeled in green and the selected sites for mutation are
marked with red star
Page 5 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
example, the carbonyl oxygen of A60 was interacted with
H62 by forming a hydrogen bond , and it will trans-
fer electrons to H62 after mutated to D60 or N60 which
might be helpful to enhance the catalytic activity. en,
10 mutants were constructed and compared with Dke1
wild-type protein. As shown in Fig. 4, the strain with
Dke1 A60D (Q3148) produced 80.2 ± 2.4 mg/L acety-
lacetone which is 2.5-fold higher than that of the strain
Q3030 (p < 0.01). e strain with Dke1 G101D (Q3151)
only produced 9.5 ± 1.4 mg/L acetylacetone while the
ability of other mutants was basically the same with wild-
type Dke1 protein (p > 0.1).
As the mutations K15Q and A60D had shown
improvement in acetylacetone production, the inﬂu-
ence of the double mutant on acetylacetone produc-
tion was also assessed. As Fig. 4 demonstrated, the
strain carrying Dke1 K15Q/A60D (Q3170) exhibited
the highest acetylacetone synthesis eﬃciency, and
115.7 ± 5.1mg/L acetylacetone was accumulated in the
culture, which was 3.6-fold higher than that of strain
Q3030 (p < 0.01). Dke1 activity was assayed in vitro
using puriﬁed proteins and the activity of each enzyme
showed a similar trend with acetylacetone production
Fig. 4 Eﬀect of Dke1 mutagenesis on acetylacetone production in ﬂask cultivation. Data were obtained after each strain was induced for 24 h in
liquid LB medium. All the experiments were carried out in triplicate. All p values are based on two-tailed tests (wild type and mutant strain) and
presented over the bars
Table 1 Analysis oftheproposed sites forsite-directed mutagenesis
Proposed sites Mutation information Annotation
A60 N60 or D60 The carbonyl oxygen of A60 (Additional ﬁle 1: Fig. S3b) is hydrogen bonded to H62. The electrons could be
transferred to H62 after Ala mutated to Asp or Asn
G101 N101, D101 or S101 The N of the main chain of G101 (Additional ﬁle 1: Fig. S3c) is hydrogen bonded to the O of the main chain
of H64. When Gly was mutated to Asp, Asn or Ser, the electron distribution of site 101 will change and
thus aﬀect the catalytic activity
L103 R103, Q103 or C103 L103 (Additional ﬁle 1: Fig. S3c) was located close to H104; the property of site 103 directly aﬀects H104.
When the nonpolar amino acid (Leu) was mutated to polar amino acid (Gln, Cys) or alkaline amino acid
(Arg), the inﬂuence to H104 from site 103 may change and thus aﬀect the catalytic activity
G105 D105 When G105 (Additional ﬁle 1: Fig. S3c) was mutated to D105, the electronegativity of the catalytic activity
center will be signiﬁcantly increased
E140 V140 E140 (Additional ﬁle 1: Fig. S3d) with hydrophobic amino acids around was located on the outer edge of the
hydrophobic channel. When the acidic amino acid (Glu) was mutated to a hydrophobic amino acid (Val), it
might be more conducive to discharge the catalytic product
Page 6 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
Structural simulation andmolecular docking ofDke1
To reveal the molecular basis for increased enzymatic
activity of Dke1 (K15Q/A60D), in silico structural mode-
ling and molecular docking were performed using the 3D
structure of Dke1 (PDB ID: 3BAL) as a model template.
As shown in Fig.5, no remarkable diﬀerences in the over-
all structure have been observed between wild-type and
mutated Dke1 protein, implying that the variant is cor-
rectly folded. Interestingly, with the mutation of K15Q,
the channel, through which substrates enter the enzyme
reactive center, was widened. Furthermore, a neutral resi-
due glutamine is less attractive to negative-charged ace-
tate than lysine which is positive charged. Taken together
both factors make it easier for acetate to arrive at the
reactive site of Dke1. e residue A60, as a hydrophobic
amino acid, is located close to the reactive center and
its side chain is oriented toward the interior of the pro-
tein. e substitution of alanine by a hydrophilic residue,
aspartate, changed the orientation of the side chain at
position 60, resulting in a reactive center with increased
volume, which may be more conducive for substrate
binding. In summary, these changes brought about by the
double mutant increased substrate access to the enzyme
active site, resulting in enhanced enzyme activity.
As the strain Q3170 presented the highest acetylacetone
production in ﬂask cultivation, fed-batch fermentation
was conducted in a 5-L bioreactor. e concentrated glu-
cose was used for cell growth and controlled between 10
and 20g/L during fermentation. 0.2mM IPTG was added
to induce the recombinant proteins when the cell den-
sity reached to about 30 OD600 at 12h. Simultaneously,
the sterilized air was switched to high-purity nitrogen
for anaerobic environments. Cell growth, residual glu-
cose and acetylacetone accumulation were monitored
over the course of the fermentation. As shown in Fig.6,
the cell mass reached to a maximum 16.8g DCW/L at
28h and decreased slightly after 36h. A small amount of
acetylacetone had already been synthesized before induc-
tion possibly due to Dke1 basal expression. After induc-
tion, acetylacetone rapidly accumulated and reached the
Table 2 Activity of the Dke1 and its mutants (p < 0.01
compared withthewild type)
Enzyme Specic activity
WT 0.8 ± 0.01 × 10−3
K15Q 1.7 ± 0.07 × 10−3
A60D 2.5 ± 0.02 × 10−3
K15Q/A60D 3.0 ± 0.07 × 10−3
Fig. 5 Analysis of the Dke1 structure with molecular docking. a Wild-type. b K15Q/A60D mutant. The β-pleated sheet shown in purple, the α-helix
shown in light blue, and the substrate channel shown in grey
Fig. 6 The time proﬁles of cell growth, residual glucose and
acetylacetone accumulation in fed-batch fermentation performed in
a 5-L laboratory bioreactor. Dry cell weight was marked with circle,
residual glucose was marked with triangle, and acetylacetone was
marked with asterisk
Page 7 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
maximum of 556.3 ± 15.2 mg/L at 48h with a yield of
5.1mmol/mol glucose. e productivity of 21.2mg/L/h
was achieved during 16–36h while only 1.1mg/L/h dur-
ing the latter 24h.
Although the yield of acetylacetone from recombinant
E. coli in this study was still relatively low, E. coli is a com-
petitive species for industrial applications with short mul-
tiplication time, growth with inexpensive carbon sources
and amenability to genetic modiﬁcation . Many strat-
egies can be used for improving the acetylacetone yield
in future study, such as metabolic ﬂow regulation 
and key genes expression level modulation . Previous
study has shown that acetylacetone has toxic side eﬀects
on the immune system of mammals , various aquatic
organisms  and microorganisms . e toxicity
threshold of acetylacetone is 67mg/L for Pseudomonas
putida , and an excess of acetylacetone (> 1.5g/L) can
completely inhibit the cell growth of A. johnsonii . We
speculated that the acetylacetone toxicity might be one of
the main reasons for the low yield. e toxicity of acety-
lacetone to E. coli cells was tested (Additional ﬁle1: Fig.
S4). e results showed that 100mg/L acetylacetone has
obvious inhibition eﬀect on the growth of E. coli, and
the cell was almost completely inhibited at a concentra-
tion of 5g/L. e mechanism of toxicity, however, has
still not been revealed. Further research is needed on
the underlying mechanism to guide the metabolic engi-
neering for higher production. Adaptive evolution is also
aneﬀective measure to improve thebacterial tolerance
and enhance the production . As E. coli naturally pro-
duces a mixture of ethanol, hydrogen, organic acids, such
as lactic acid, succinic acid, acetic acid under anaerobic
conditions , metabolic ﬂow analysis and regulation
are necessary to conduct for acetylacetone production
enhancement. In addition, as the cultivation conditions
play an important role on cell growth, product synthesis,
and conversion eﬃciency, it is also worthy to optimize
the medium components, substrate addition strategy, fer-
mentation mode, etc. in future research.
e biosynthetic pathway of acetylacetone was con-
structed, and the acetylacetone was produced
successfully from glucose by engineered E. coli by over-
expression of acetylacetone-cleaving enzyme (Dke1).
e production was improved by site-directed mutagen-
esis of Dke1 and the double mutant (K15Q/A60D) ena-
bled the highest acetylacetone-producing capacity.
Finally, 556.3 ± 15.2 mg/L acetylacetone was obtained
at 36h post-induction in fed-batch fermentation under
anaerobic condition. is study presents the ﬁrst intui-
tive biosynthetic pathway of acetylacetone inspired by
its biodegradation, and shows its potential for large-scale
production. As reported, Dke1 is not absolutely speciﬁc
for acetylacetone. Many related β-dicarbonyl compounds
such as 3,5-heptanedione, 2,4-octanedione, 2-acetylcy-
clohexanone, ethylacetoacetate, etc. can be accepted as
substrate by Dke1 [8, 9]. As a consequence, the results of
this study also provide a possible biosynthesis method for
other β-dicarbonyl compounds.
All strains and plasmids used in this study are listed in
Table3. e primers used for plasmid construction and
allele veriﬁcation are listed in Additional ﬁle2: TableS2.
E. coli DH5α (Invitrogen) was used for plasmids’ prepa-
ration and BL21 (DE3) was used for recombinant pro-
tein expression and acetylacetone production. e genes
encoding acetylacetone-cleaving enzyme from A. john-
sonii, Paraburkholderia xenovorans LB400, and Tistrella
mobilis were codon optimized and chemically synthe-
sized by BeijingLiuheBGI, and cloned into pETDuet-1
between EcoRI and BamHI sites to construct plasmids
pETDuet-Dke1, pETDuet-P_Dke1, and pETDuet-T_
Dke1, respectively. Mutations were introduced into the
Dke1 gene in pETDuet-Dke1 using an overlap extension
PCR method . e resulting plasmids were named
pM1 to pM19, respectively. All the recombinant plasmids
were veriﬁed by colony PCR and nucleotide sequencing.
Protein expression andgel electrophoresis analysis
To check the expression of the recombinant proteins,
single colonies of E. coli BL21 (DE3) harboring diﬀer-
ent recombinant plasmids were cultured in LB medium
containing appropriate antibiotics at 37°C overnight and
then diluted 1:100 into fresh LB medium and induced
with 0.2 mM isopropyl-β--thiogalactopyranoside
(IPTG) at an OD600 of 0.6–0.8. e cells were collected
from 10 mL bacteria cultures 4 h post-induction and
washed with phosphate buﬀer (pH 6.8). e washed cells
were suspended in 1mL buﬀer and subjected to ultra-
sonication (SCIENTZ JY92-IIN, 300W, 3s pulse on and
3s pulse oﬀ for 5min). e cell lysates were centrifuged
and the protein expression was analyzed by SDS-PAGE.
Dke1 activity assay
e invitro reaction system (1mL) for the activity assay
of Dke1 contained 0.1 M puriﬁed enzyme, 0.5 mM
FeSO4·7H2O, 10mM MgCl2·6H2O, 10mM KCl, 1mM
DTT, 10 mM methylglyoxal and 10 mM ammonium
acetate in 20mM Tris/HCl buﬀer (pH 7.5) and was incu-
bated at 37°C for 24h. e reaction mixture was centri-
fuged at 5000×g for 5min, and then the supernatant was
subjected to HPLC or GC–MS analysis to verify acetylac-
Page 8 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
To evaluate the acetylacetone production using diﬀerent
engineered strains, shake-ﬂask cultivations were carried
out with 50mL of liquid LB medium containing 20g/L
glucose in 250-mL non-baﬄed ﬂasks or serum bottles
with appropriate antibiotics. When necessary, ampicil-
lin and chloramphenicol were added at a ﬁnal concen-
tration of 100 g/mL and 50 g/mL, respectively. e
strains were inoculated to the medium and incubated in
an orbital shaker incubator at 37°C and 180rpm. 0.2mM
Table 3 Strains andplasmids used inthis study
Strains orplasmids Genotype/description Source
E. coli DH5 F− recA endA1 Φ80dlacZΔM15 hsdR17(rK
E. coli BL21 (DE3) F− ompT hsdSB (rB
−) gal dcm λ (DE3) Invitrogen
Q2837 E. coli BL21(DE3) carrying pETDuet-Dke1 and pACYCDuet-MgsA-TpiA This study
Q3028 E. coli BL21(DE3) carrying pETDuet-Tmo_Dke1 This study
Q3029 E. coli BL21(DE3) carrying pETDuet-Pxe_Dke1 This study
Q3030 E. coli BL21(DE3) carrying pETDuet-Dke1 This study
Q3080 E. coli BL21(DE3) carrying pM1 This study
Q3081 E. coli BL21(DE3) carrying pM2 This study
Q3082 E. coli BL21(DE3) carrying pM3 This study
Q3085 E. coli BL21(DE3) carrying pM6 This study
Q3086 E. coli BL21(DE3) carrying pM7 This study
Q3148 E. coli BL21(DE3) carrying pM9 This study
Q3149 E. coli BL21(DE3) carrying pM10 This study
Q3150 E.coli BL21(DE3) carrying pM11 This study
Q3151 E. coli BL21(DE3) carrying pM12 This study
Q3152 E. coli BL21(DE3) carrying pM13 This study
Q3153 E. coli BL21(DE3) carrying pM14 This study
Q3154 E. coli BL21(DE3) carrying pM15 This study
Q3155 E. coli BL21(DE3) carrying pM16 This study
Q3170 E. coli BL21(DE3) carrying pM17This study
pETDuet-1 AmpR, reppBR322, lacI PT7 Novagen
pACYCDuet-1 CmR, p15A origin, lacI PT7 Novagen
pETDuet-Dke1 pETDuet-1 harboring acetylacetone-cleaving enzyme (Dke1) from A. johnsonii This study
pETDuet-Tmo_Dke1 pETDuet-1 harboring acetylacetone-cleaving enzyme from T. mobilis This study
pETDuet-Pxe_Dke1 pETDuet-1 harboring acetylacetone-cleaving enzyme from P. xenovorans This study
pACYCDuet-MgsA-TpiA pACYCDuet-1 harboring methylglyoxal synthase (MgsA) and triose-phosphate isomerase
(TpiA) from E. coli
pM1 reppBR322 AmpR lacI PT7 Dke1K15Q This study
pM2 reppBR322 AmpR lacI PT7 Dke1S17D This study
pM3 reppBR322 AmpR lacI PT7 Dke1Y21W This study
pM6 reppBR322 AmpR lacI PT7 Dke1L103R This study
pM7 reppBR322 AmpR lacI PT7 Dke1G105D This study
pM9 reppBR322 AmpR lacI PT7 Dke1A60D This study
pM10 reppBR322 AmpR lacI PT7 Dke1A60N This study
pM11 reppBR322 AmpR lacI PT7 Dke1G101N This study
pM12 reppBR322 AmpR lacI PT7 Dke1G101D This study
pM13 reppBR322 AmpR lacI PT7 Dke1G101S This study
pM14 reppBR322 AmpR lacI PT7 Dke1L103C This study
pM15 reppBR322 AmpR lacI PT7 Dke1L103Q This study
pM16 reppBR322 AmpR lacI PT7 Dke1E140V This study
pM17 reppBR322 AmpR lacI PT7 Dke1K15Q/A60D This study
Page 9 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
IPTG was added into the medium to induce the recombi-
nant protein expression when the cells reached at about
0.6–0.8 OD600. Nitrogen protection was introduced to
create an anaerobic environment in the serum bottle.
After induction, the temperature was set at 30°C for fur-
ther 24h cultivation. All shake-ﬂask experiments were
performed in triplicate.
For large-scale production, fed-batch fermentation was
carried out in a Biostat B plus MO5L bioreactor (Sarto-
rius Stedim Biotech GmbH, Germany) containing 2 L
growth medium (20g/L tryptone, 10g/L yeast extract,
20 g/L NaCl, 3 g/L KH2PO4, 0.26g/L MgSO4, 1.0g/L
NH4Cl, 15.2 g/L Na2HPO4, 20 g/L glucose and 2 mL
of trace element solution). e trace element solution
contained (per liter) 3.7 g (NH4)6Mo7O24·4H2O, 2.47g
H3BO3, 1.58 g MnCl2·4H2O, 0.29 g ZnSO4·7H2O, and
0.25 g CuSO4·5H2O. Two hundred milliliters of over-
night seed culture was inoculated into the bioreactor to
start the fermentation at 37°C. During the fermentation,
sterilized air was supplied at 1 vvm and ammonia was
added automatically to control the pH 7. e agitation
speed was set at 400 rpm and then associated with the
dissolved oxygen to maintain the concentration at 20%
saturation. Fed-batch mode was commenced by feeding
60% glucose when the dissolved oxygen increased. When
the cell density reached to an OD600 of 30, the recombi-
nant proteins were induced by 0.2mM IPTG along with
0.5mM FeSO4·7H2O added, and nitrogen was used for
anaerobic conditioning after induction. e temperature
was adjusted to 30°C for further cultivation. e agita-
tion speed was kept at a constant rate of 200rpm dur-
ing anaerobic fermentation. More details of the fed-batch
fermentation are presented in Additional ﬁle1: Fig. S5
and Additional ﬁle2: Fed-batch fermentation. 5mL of the
fermentation broth was withdrawn periodically to deter-
mine the cell density, residual glucose and product titer.
e fed-batch fermentation was performed in triplicate.
Molecular docking of acetylacetone with Dke1 was car-
ried out with AutoDock 4.2.6 program. e initial struc-
ture was prepared using AutoDockTools 1.5.6 ,
preserving the original charge of the protein and generat-
ing a pdbqt ﬁle for docking. e 3D structure of the acet-
ylacetone was downloaded from the PubChem database.
MOPAC program was then used to optimize the struc-
ture and calculate the PM3 atomic charge. e structure
of acetylacetone was also prepared by AutoDock Tools
1.5.6, and the corresponding pdbqt ﬁle was generated
for docking. e active site of Dke1 was chosen as the
binding pocket for docking. e number of grid points
in the XYZ of grid box was set to 40 × 40 × 40, the grid
spacing was 0.375Å, the number of Genetic Algorithm
(GA) run was set to 100, and the rest parameters were set
to default. Finally, the structure with the lowest docking
energy was carried out with energy minimization. e
optimization process is carried out in two steps: ﬁrst, the
steepest descent method optimization of 2000 steps, then
the structure was further optimized by the 2000 steps
with conjugate gradient method.
e OD at 600nm was routinely used to monitor cell
growth via ultraviolet spectrophotometer (Varian Cary
50 UV–Vis, US), and one unit of OD600 was equivalent
to 0.43g DCW/L . e residual glucose was deter-
mined by an SBA-40D biosensor analyzer (Institute of
Biology, Shandong Academy of sciences, China). e
culture samples were centrifuged at 10,000×g for 10min;
the supernatants were ﬁltered through a 0.2-M Tuﬀryn
membrane (China) and used for HPLC analysis (Waters
1525, 300 mm × 7.8 mm Aminex HPX-87H, UV–Vis
detector at 280 nm, for more detailed information see
Additional ﬁle1: Fig. S6 and Additional ﬁle2: Standard
curve establishment and TableS3). To further conﬁrm
acetylacetone production in our cultures, the same sam-
ple was analyzed by GC–MS after HPLC determination.
e GC–MS analysis was performed with an Agilent
GC quadrupole instrument. e analysis conditions
were as follows: a 30-m HP-5 column (internal diameter
0.25mm, ﬁlm thickness 0.25m), the column tempera-
ture program was composed of an initial hold at 50°C for
5min, ramping at 15°C per min to 240°C and holding
for 5min. e injector and transfer line temperature were
240 and 250°C, respectively. e mass spectrometry full
scan was from 30 to 400, the ion source and quadrupole
temperature were 230 and 150°C, respectively.
Supplementary information accompanies this paper at https ://doi.
org/10.1186/s1306 8-020-01725 -9.
Additional le1. Additional ﬁgures.
Additional le2. Additional tables.
Dke1: Acetylacetone-cleaving enzyme; TpiA: Triose-phosphate isomerase;
MgsA: Methylglyoxal synthase; E. coli: Escherichia coli; A. johnsonii: Acinetobacter
johnsonii; GC–MS: Gas chromatography–mass spectrometer; HPLC: High-
performance liquid chromatography; OD600: Optical density at wavelength
600 nm; DTT: Dithiothreitol; IPTG: Isopropyl-β-
-thiogalactoside; DCW: Dry cell
We thank Miss Yingxin Fu (QIBEBT, CAS) for her work on the additional acety-
lacetone tolerance experiment, especially she has overcome many diﬃculties
that is being caused by the COVID-19 pandemic.
Page 10 of 10
Zhouetal. Biotechnol Biofuels (2020) 13:88
XF and GZ designed the study. YZ, WG, JW, and XL carried out the experi-
ments. GZ, XF, YD, XM, and YZ contributed to discuss and analyze the data. XF,
GZ, YZ, and MX wrote the manuscript. All authors read and approved the ﬁnal
This work was supported by the National Defense Science and Technology
Innovation Zone Foundation of China, National Natural Science Foundation
of China (31722001, 31670089, and 31800081), Taishan Scholars Program of
Shandong Province (ts201712076), Natural Science Foundation of Shandong
(JQ201707 and ZR2019QB015), and the Youth Innovation Promotion Associa-
tion at the Chinese Academy of Sciences.
Availability of data and materials
We provide all the necessary data for the publication of this article. All addi-
tional data are present in the article and the additional material documents.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
1 CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy
and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101,
China. 2 University of Chinese Academy of Sciences, Beijing 100049, China.
3 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,
China. 4 State Key Laboratory of Microbial Technology, Shandong University,
Qingdao 266237, China.
Received: 12 March 2020 Accepted: 7 May 2020
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