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Dragon's Blood from Dracaena cambodiana in China: Applied History and Induction Techniques Toward Formation Mechanism

Authors:
  • Guangxi Minzu University

Abstract

Dragon's blood that is extracted from Dracaena plants has been widely used as traditional medicine in various ancient cultures. The application of dragon's blood has a cherished history in China, even though the original plants were not discovered for some period. Dracaena cochinchinensis and Dracaena cambodiana were successively discovered in southern China during the 1970s-1980s. In the last half of the century, Chinese scientists have extensively investigated the production of dragon's blood from these two Dracaena species, whereas these results have not been previously systematically summarized, as in the present paper. Herein, we present the applied history in ancient China and artificially induced technologies for dragon's blood development based on these two Dracaena species, in particular, using tissue cultures seedlings and tender plants of D. cambodiana. Big data research, including transcriptomic and genomic studies, has suggested that dragon's blood might be a defense substance that is secreted by Dracaena plants in response to (a)biotic stimuli. This review represents an effort to highlight the progress and achievements from applied history as well as induction techniques that are used for the formation of dragon's blood that have taken place in China. Such knowledge might aid in the global conservation of wild Dracaena species and contribute to understanding dragon blood formation mechanisms, eventually assisting in the efficient utilization of limited Dracaena plant resources for the sustainable production of dragon's blood.
Forests 2020, 11, 372; doi:10.3390/f11040372 www.mdpi.com/journal/forests
Review
Dragon’s Blood from Dracaena cambodiana
in China: Applied History and Induction Techniques
Toward Formation Mechanism
Xupo Ding 1,2, Jiahong Zhu 2, Hao Wang 1, Huiqin Chen 1, and Wenli Mei 1,2,*
1 Hainan Key Laboratory for Research and Development of Natural Products from Li folk Medicine,
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences,
No. 4 Xueyuan Road, Haikou 571101, Hainan, China; dingxupo@itbb.org.cn (X.D.); wanghao@itbb.org.cn
(H.W.); chenhuiqin@itbb.org.cn (H.C.)
2 Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture and Rural
Affairs, Institute of Tropical Bioscience and Biotechnology; Chinese Academy of Tropical Agriculture
Sciences, No. 4 Xueyuan Road, Haikou 571101, Hainan, China; zhujiahong@itbb.org.cn
* Correspondence: meiwenli@itbb.org.cn; Tel.: +86-898-6698-7529
Received: 19 January 2020; Accepted: 25 March 2020; Published: 26 March 2020
Abstract: Dragons blood that is extracted from Dracaena plants has been widely used as traditional
medicine in various ancient cultures. The application of dragons blood has a cherished history in
China, even though the original plants were not discovered for some period. Dracaena
cochinchinensis and Dracaena cambodiana were successively discovered in southern China during the
1970s1980s. In the last half of the century, Chinese scientists have extensively investigated the
production of dragons blood from these two Dracaena species, whereas these results have not been
previously systematically summarized, as in the present paper. Herein, we present the applied
history in ancient China and artificially induced technologies for dragons blood development based
on these two Dracaena species, in particular, using tissue cultures seedlings and tender plants of D.
cambodiana. Big data research, including transcriptomic and genomic studies, has suggested that
dragon’s blood might be a defense substance that is secreted by Dracaena plants in response to
(a)biotic stimuli. This review represents an effort to highlight the progress and achievements from
applied history as well as induction techniques that are used for the formation of dragons blood
that have taken place in China. Such knowledge might aid in the global conservation of wild
Dracaena species and contribute to understanding dragon blood formation mechanisms, eventually
assisting in the efficient utilization of limited Dracaena plant resources for the sustainable production
of dragon’s blood.
Keywords: dragon’s blood; Dracaena cambodiana; big data; genetic background; formation
mechanism; organelle genome; survey sequencing
1. Introduction
Dragons blood, a crimson resin that is exuded from the injured branch or trunk of Dracaena
plants from Asparagaceae family [1,2], has been broadly utilized in the histories of many cultures as
a traditional medicine for curing fractures, wounds, diarrhea, piles, and stomach and intestinal ulcers
[3,4]. Modern pharmacological research has demonstrated that dragons blood also has anti-
inflammatory, antimicrobial, antioxidant, antitumor, and cytotoxic activities [4]. Further studies have
indicated that flavonoids, saponins, terpenes, and steroids that are biosynthesized in plant secondary
metabolism pathways are some of the main pharmacodynamic compounds found in dragons blood
[510].
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The sources and identification of dragon’s blood have always generated great confusion. Being
used as a traditional name for many different resins, dragon’s blood has been described in the medical
literature as being obtained from the stems of Dracaena draco (L.) L, Dracaena cinnabari Balf.f,
Pterocarpus draco L., Croton lechlerill.Arg., Croton gossypifolium Vahl, and the fruit of Daemonorops
draco (Willd.) Blume, in these cases being respectively referred to as Canary dragons blood, Socotran
dragon’s blood, West Indian dragons blood, Mexican dragons blood, Venezuelan dragons blood,
and East Indian dragons blood [11,12], but dragons blood was originally produced from D.cinnabari,
later from Dracaena draco and more recently from Daemonorops draco [4,13]. In China, dragons blood
had always been considered to be exotic before the source species were discovered in Yunnan
Province of China. Even now, a large proportion of dragons blood used in China is dependent on
import from the countries of Southeast Asia.
Resource shortages and medical requirements have resulted in an abundance of research in the
last half-century regarding the production of dragons blood based on limited Dracaena plants. Here,
we elaborate on the history regarding the discovery and exploitation of dragon’s blood in China, and
the two species from which it was first obtained. Subsequently, we outline the artificial inducing
techniques for dragon’s blood formation, based on D. cochinchinensis (Lour.) S.C. Chen and D.
cambodiana Pierre ex Gagnep and developed by Chinese scientists, in particular, for those
technologies that are involved in the plant tissue cultures seedling of D. cambodiana for producing
dragon’s blood. Most importantly, we examine the current transcriptomic and survey genomic
approaches that are used to study the mechanisms of dragons blood formation in D. cambodiana
plants in China.
2. The History of Dragons Blood as a Traditional Chinese Medicine in China
De Materia Medica, edited by Pedanius Dioscorides (approximately 4090 AD), who was a Greek
physician, pharmacologist, and botanist, lists the earliest record of dragon’s blood and Dracaena
plants [14]. He first encountered Dracaena plants and the production of dragons blood in the Middle
East and North African regions while he was employed as a physician in the Roman army. Therefore,
the history of dragons blood use might pre-date the first century in these local ancient cultures.
Dragons blood use as a traditional medicine in China can be dated from the fifth century AD, which
is later than its use by Greeks and Egyptians, on account of the lack of source plants. Dragons blood
was first documented in “Lei Gongs Treatise on Preparation and Boiling of Materia Medica (Lei Gong
Pao Zhi Lun)” by Xiao Lei in the Liu-Song Period (approximately 420479 AD) of the Northern and
Southern Dynasties of China; the pharmacologists of the time considered dragons blood to have the
functions of promoting blood circulation and removing stasis in blood, healing bleeding and sores,
and to function as an anti-inflammatory and analgesic. However, the source plant was not described
until approximately the 1060s AD during the Northern Song Dynasty, in which “Illustrated Classics
of Materia Medica (Ben Cao Tu Jing)” of Song Su appears to have been published. Furthermore,
dragon’s blood was also listed in the New Revised Materia Medica (Xin Xiu Ben Cao), Chu-Fan Chih
(Zhu Fan Zhi), and Materia Medica of South Yunnan (Dian Nan Ben Cao), all of which are classical
traditional Chinese medicine (TCM) records. The most famous of these is the Compendium of
Materia Medica, edited by Shizhen Li, who was the most distinguished pharmacist in ancient China
and East Asia, confirming that dragons blood was an effective medicine for stimulating blood
circulation [15]. Modern natural production chemistry and pharmacological researches has indicated
that the monomeric compounds extracted from dragon’s blood also have anti-inflammatory,
antimicrobial, antioxidant, antitumor, and cytotoxic activities, such as Loureisin A, Loureisin B, 7,4’-
dihydroxyflavone, and 5,7,4’- trihydroxyflavone [69]. The first source species of dragon’s blood
were Dracaena plants in ancient China, followed by Daemonorops draco from Southeast Asia [4,13].
Even now, the fruits of exotic Daemonorops draco represent the main source of dragon’s blood in China
[16]. Excitement arose when two source species for dragons blood, D.cochinchinensis and
D.cambodiana, were found in China, and scientific research that was based on these two species began
in the 1970s. Wild Dracaena plants have been excessively exploited due to their medicinal and
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economic importance, and many of them have been considered to be endangered [17], including
D.cochinchinenesis and D. cambodiana.
3. The Dracaena Source Species and Their Conservation in China
3.1. Dracaena cochinchinensis
In 1972, the team of Prof. Xitao Cai from Kunming Institute of Botany, Chinese Academy of
Sciences, found D. cochinchinensis in Yunnan Province of China and determined its dragons blood to
have equal medicinal efficacy to that sourced from other Dracaena species that were in use. The
sapling of D. cochinchinensis can increase by around 1020 cm in nature, or approximately 2329 cm
under cultivation conditions, every year [18]. The ensuing investigation indicated that D.
cochinchinensis was distributed in the subtropical and tropical regions between approximately 21.5°
23.6° N, such as in southern China, Vietnam, Cambodia, and Laos [19]. A large number of D.
cochinchinensis plants were found in southern Yunnan and Guangxi Province of China in the 1980s,
being distributed at altitudes that ranged from approximately 400 to 1700 m. Subsequently, the
dragon’s blood from D. cochinchinensis and its associated medicines were also developed and named
as dragons blood of Guangxi Xue Jie or Long Xue Jie [20].
3.2. Dracaena cambodiana
Dracaena cambodiana was discovered in China during the investigation of sustainable traditional
medicine resources in the 1980s, and this Dracaena species was distributed in jungle, stone cracks,
cliffs, or desert islands in Hainan Province and southeast Asia, for instance, in Vietnam and
Cambodia [21]. As a cork tree, the D. cambodiana plant could not be used as firewood or furniture,
and a significant level of moisture was stored in its stem and branch, thus showing excellent drought
tolerance. These immense adaptations indicated that the population of D. cambodiana predominantly
formed in or around tropical forests and the individuals of D.cambodiana tree are often several
hundred years old [18].
3.3. Conservation of D. cochinchinensis and D. cambodiana in China
Dracaena plants have been exhausted for their economic and medicinal importance in the period
of 1980s ~ 2000s, and many of them were considered to be endangered. In 1998, many of the Dracaena
species were list in the Red List of IUCN (The International Union for Conservation of Nature) [22
24]. Soon afterwards, wild D.cochinchinensis and D.cambodiana species in China had been list as
endangered species in 2001 and were then prohibited to felling. The stem of these two Dracaena
species thicken slowly and only the 30~50 years old trees can produce a small amount of dragon's
blood [25]. There are the two main causes of raw material of dragon's blood in China relying on
import commerce. D.cambodiana is also a horticultural plant species and its tender plant is widely
cultured in chamber as bonsai or planted in courtyard as an ornamental tree [26]. The scientists have
been focusing on producing dragon's blood while using these tender of D.cambodiana since Dracaena
plants discovered in China and many inducing experiments have been performed. These artificial
inducing technologies might contribute to reduce the demand for wild harvested dragon’s blood.
4. The Technology for Artificial Inducing Dragons Blood Formation
4.1. Wounds and Dragon's Blood Formation
The initial research of Prof. Cai revealed that artificial wounds could promote red resin
accumulation in the stem of D. cochinchinensis; hence, the wound became a controlled experiment for
further studies on dragon’s blood formation in Dracaena species that is still used now. This dragons
blood has both similar chemical constituents and clinical curative effect as dragons blood from
Africa, but its constituents are different to dragons blood from Southeast Asia, although it has same
clinical efficacy as Indonesian dragons blood that is extracted from the fruit of Daemonorops species
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[27]. Further textual criticism demonstrated that the dragons blood that was used throughout the
ancient history of Traditional Chinese Medicine was extracted from the Dracaena species planted in
the regions of Africa or West Asia, and that dragons blood from Daemonorops species was the
succedaneum of African dragons blood [28,29].
4.2. Microorganisms and Dragons Blood Formation
Previous studies in model plants have indicated that defense components, such as benzoxazine,
camalexins, capsidiol, momilactone, piceids, sakuranetin, and resveratrol, can be induced by
microorganisms, and that many of them are flavonoids [30–34]. Modern natural product chemistry
has demonstrated that dragons blood is composed of flavonoids, sterol, lignin, stilbene, and saponin,
and that flavonoids are the major constituents [35–37]. Microorganisms may also be involved in the
formation of dragon’s blood. The earliest studies on the relationship between microorganisms and
dragon’s blood focused on D. cochinchinensis. 303 fungi strains, divided into 23 genera, were isolated
from xylem containing dragons blood from D. cochinchinensis plants, and 52% of strains were
Fusarium species. Of these Fusarium strains, 38% were identified as F. graminearum Schw. The other
Fusarium strains were F.culmorum (W.G.M.) Sacc. (20%), F. tricinctum (Cd.) Snyd. et Hans (18%), F.
solani f. sp. pisi (13%), F. sporotrichioides Sherb. (10%), and with an isolation frequency below 5% were
Fusarium strains F. oxysporum Schl., F. moniliforme Sheld., and F. lateritium (Nees) emend. Snyder and
Hansen. Although Aureobasidium and Cladosporium strains were also isolated, the Fusarium genus was
considered as the predominant strain on account of its high isolation frequency, and subsequent
studies also revealed that the Fusarium species could induce dragon’s blood formation in D.
cochinchinensis stems [38]. Furthermore, 172 fungi strains were isolated from the leaf, stem, or roots
of D. cambodiana, with the stem showing the highest abundance of Fusarium species [39].
After being infected by F. graminum at 0, 5, 10, and 15 days, 7,4′-dihydroxyflavanone, 7-hydroxy-
4′-methoxyflavane, and loureirin A-the main active components of dragons blood from D.
cochinchinensiscontinuously accumulated in the stems of D. cochinchinensis-7,4′-
dihydroxyflavanone synthesis occurred later than that of 7-hydroxy-4′-methoxyflavane and loureirin
A, but its content was the highest. Six months later, the yield of dragons blood increased to 67%–
120% [38]. F. proliferatum and F. oxysporum can also induce dragons blood formation in the stems of
D. cochinchinensis and D. cambodiana. When compared to the wounded only control, the application
of two strains of F. proliferatum increased the yield of dragons blood in the stem of D. cochinchinensis
plants by 2.7 and 3.3 times [40], and it effectively elicited dragons blood formation from the
surrounding leaves and in the inoculation spots in D. cambodiana [41]. Their chemical fingerprint
showed that the components of artificial dragons blood from Fusarium species induction were similar
to natural dragons blood [40,41]. Since then, in about 2007, research on dragons blood in China
gradually shifted its focus to D. cambodiana plant, as it is easier to cultivate than D. cochinchinensis
plant.
4.3. Plant Hormones and Dragons Blood Formation
Microorganism infection can activate plant hormone synthesis and increase endogenous
hormone content, and exogenous hormones might also induce dragons blood formation in D.
cambodiana. The production of dragons blood increased by 2.57, 1.6, 2.64, and 4.57 times in response
to treatment with gibberellin (GA), indole-3-acetic acid (IAA), brassinolide (BR), and kinetin (KT),
respectively. Furthermore, the combination of any two of these three plant hormones can also lead to
synergistically increased dragons blood formation. The application of two of the most important
hormones in plants, jasmonic acid (JA) and salicylic acid (SA), did not result in any increases in the
yield of dragons blood at any of the tested concentrations. In addition, 2,4-dichlorophenoxyacetic
acid (2,4-D) was demonstrated to have a lethal effect on D. cambodiana plants in experiments to induce
dragon’s blood formation [42]. The application of the common cytokinin 6-benzylaminopurine (6-
BA) could also significantly induce dragon’s blood production in the branch or stem of three-year old
D. cambodiana plants. In experiments examining the interaction of plant growth regulators (PGRs)
with tissue-cultured seedlings of D. cambodiana, KT, GA, IAA, 2,4-D, and 1-naphthylacetic acid (NAA)
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were respectively mixed in MurashigeSkoog (MS) medium. In tissue cultured seedlings, only 6-BA
could promote the secretion of loureirin A and loureirin B into MS medium in culture bottles [43],
and this effect did not appear to be dose-dependent, but rather illumination intensity-dependent. In
the case of tissue-cultured seedlings of D. cambodiana, none of the PGR treatments resulted in the
secretion of dragons blood into medium under dark conditions, including 6-BA, which was able to
induce tissue-cultured seedlings of D. cambodiana in order to secrete dragon’s blood into medium
under illumination intensities of 10003000 Lx, and the inducing effect was positively related to
illumination intensity [43].
4.4. Small Molecules and Dragons Blood Formation
Being inspired by the induction of exogenous plant hormones, chemical molecules might
induce dragons blood formation more immediately according to their small MW (molecular weight).
Seventeen small molecules (analytical reagent), including oxalic acid (H2C2O4), sodium oxalate
(Na2C2O4), zinc sulfate (ZnSO4), magnesium nitrate (Mg(NO3)2·6H2O), hydrochloric acid (HCl),
leucine (Leu), sodium thiosulfate (Na2S2O3·5H2O), sodium 2-nitrophenoxide, sodium 4-
nitrophenoxide, sodium 2-methoxy-5-nitrophenol, compound sodium nitrophenolate, hydrogen
peroxide (H2O2), sodium bromide (Na2Br·2H2O), sodium molybdate (Na2MoO4), copper sulfate
(CuSO4·5H2O), barium chloride (BaCl2), and sodium pyrophosphate (Na4P2O7), were used as
chemical inducers on D. cambodiana plants, and three concentration (10%, 1%, and 0.1%) were tested,
followed by the harvesting of dragons blood for counting three months after treatment. The statistics
show that > 100% induction was observed for H2C2O4 (1%), ZnSO4, Mg(NO3)2 (0.1%), sodium 4-
nitrophenoxide (0.1%), Leu (10%), and H2C2O4 (10%), while ZnSO4 (10% and 0.1%), Mg(NO3)2 (10%
and 1%), and sodium 2-nitrophenoxide (0.1%) could induce a > 50% and < 100% increase in the yield
of dragons blood. By contrast, Na2Br, Na2MoO4, CuSO4, and BaCl2 at 10% concentration decreased
the dragons blood formation [42]. These results suggest that acid and sodium salt can significantly
increase the accumulation of dragon’s blood. Subsequent research also found that compounds that
are mixed with sodium chloride (NaCl) and acetic acid (HOAc) can induce dragons blood formation
in the stem of D. cambodiana rapidly, and even flavanones-the special constituents of dragons blood-
could be detected using high-performance liquid chromatography (HPLC) after treatment for nine
days [44], in particular, (2S)7,4’-dihydroxyflavanone, 4,4′-dihydroxy-2-methoxydihydrochalcone,
(2S)-7,3’-dihydroxy-4’-methoxyflavane, (2R)-7,4’-dihydroxy-8-methylflavane, (2S)-3’,7-dihydroxy-
4’-methoxy-8-methylflavane, or (3R)-3,5,7-trihydroxy-4’-methoxy-6-methoxydihydro-
homoisoflavone [45]. Based on this phenomenon, the mechanism of dragons blood formation was
preliminarily explored while using transcriptome analysis.
5. The Mechanism of Dragons Blood Formation
5.1. Suppression Subtractive Hybridization
Before the large-scale application of transcriptomics, suppression subtractive hybridization
(SSH) was the popular technology in molecular biology for priming genes with different expression
levels [46]. In 2005, 431 recombinants were obtained through SSH by comparing the samples with
and without the production of dragons blood in D. cambodiana tissue culture plantlets. Subsequently,
51 fragments showing differential expression were indicated via sequencing and reverse Northern
blot. Of these 51 fragments, 12 cDNA fragments were related to metabolism and energy transfer, five
cDNA fragments were involved in signaling transduction, five cDNA fragment were related to
transcription and translation, one cDNA fragment was related to photosynthesis, and the other 23
cDNAs were not homologous to genes in the Nr database [47]. In this SSH library, the complete open
reading frame encoding calcium-dependent protein kinase 2 (CDPK2) was cloned with RACE, and
its expression profile was found to be consistent with dragons blood accumulation in the stem of D.
cambodiana via semi-quantitative RT-PCR. Now we know that the CDPKs play vital roles in plant
hormone signaling, growth and development, plant (a)biotic stress responses [48], and they even
contribute to plant secondary metabolism by activating calcium binding to their calmodulin
Forests 2020, 11, 372 6 of 13
regulatory domains [49,50]. Frustratingly, the genes that are directly related to flavonoid or terpene
synthesis in plants were not detected by SSH [47].
5.2. Transcriptome
In 2014, the transcriptome for the accumulation of dragon’s blood was generated while using
the stems of three-year-old D. cambodiana plants [44]. Three cDNA libraries were constructed, with
the stems being injected by the inducer on the D. cambodiana plant at 0, 3, and 6 days, and 266.57
million raw data from Illumina HiSeq 2000 were assembled into 198,204 unigenes while using Trinity,
of which 34,873 unigenes were annotated in the public database. Totals of 2724, 1698, and 2155
unigenes were respectively expressed in the treatments for 0, 3, and 6 days, and the DESeq
determined 6986, 7106, and 6085 differentially expressed genes (DEGs) corresponding to the
respective pairs of 0 and 3 days, 0 and 6 days, and 3 and 6 days. The KEGG (Kyoto Encyclopedia of
Genes and Genomes) classification found 76 DEGs to be involved in flavonoid biosynthesis from the
phenylpropanoid pathway, including phenylalanine ammonia-lyase (PAL, six DEGs), cinnamate-4-
hydroxylase (C4H, one DEG), 4-coumarate CoA ligase (4CL, 18 DEGs), chalcone synthase (CHS, 10
DEGs), chalcone isomerase (CHI, six DEGs), flavanone 3-hydroxylase (F3H, seven DEGs), flavonol
synthase (FLS, 10 DEGs), dihydroflavonol 4-reductase (DFR, 16 DEGs), and leucoanthocyanidin
reductase (LAR, one DEG), and of these, 34 DEGs are involved in the catalysis of flavanones into
flavonols [44]. As another important chemical component, the total concentration of steroidal
saponin, was significantly decreased during dragons blood formation, and we also detected 122
unigenes that were involved in steroidal saponin biosynthesis, 29 of which encoded 24 kinds of
enzymes that had complete open reading frames (ORF) and differential expression [51]. The plant
chemical diversity of flavonoids and saponin is dependent on their modification through
methylation, glycosylation, or hydroxylation. A total of 27 unigenes encoding O-methyltransferase
(OMT, 2 DEGs), UDP-glycosyltransferase (UGT, 14 DEGs), or cytochrome P450 (CYP450, 11 DEGs)
were significantly upregulated upon treatment with inducer [44].
Flavonoids are synthesized in the plant cytosol [52], whereas the precursors of steroidal saponin
can be synthesized in cytosol or plastid [53]. After synthesis, they are all transported into vacuoles
for storage, or to other destinations [54]. The analysis of transcript expression demonstrated that 13
DEGs encoding multidrug resistance-associated proteins (ABC, MRP-type) were significantly
induced, while only three DEGs encode ATP-binding cassette transporters (ABC, G-type)the
results might suggest that MRP-type ABC transporters play key roles in secondary metabolism
transport during the accumulation of dragons blood. Furthermore, two DEGs encoding H+PPase,
two DEGs encoding vacuolar sorting receptor (VSR), four DEGs encoding soluble N-ethylmaleimide-
sensitive factor attachment protein receptors (SNARE), 18 DEGs encoding H+ATPase, and 18 DEGs
encoding multidrug and toxic compound extrusion protein transporters (MATE) were also found to
be significantly upregulated in dragons blood formation [44]. Previous studies have indicated that
glutathione S-transferase (GST) genes contribute to the transportation of flavonoids from the site of
cytosolic synthesis to vacuolar accumulation in plant cells. A total of 20 GST genes were identified
based on the transcriptome database, and their transcript profiles were strongly correlated with those
of genes that were involved in dragon’s blood formation and flavonoid synthesis [55].
As essential transcription factors (TFs), MYB, bHLH, and WD40 play essential roles in flavonoid
synthesis and transport [56]. Our transcriptomic analysis also detected 86 TFs with differential
expression profiles in the process of dragons blood accumulation, including 41 DEGs of MYB, 33
DEGs of bHLH, and 12 DEGs of WD40, whereas not all of the TFs selected were induced [41]. The
ternary complex of MYB–bHLH–WD40 generally regulating the expression of numerous structural
genes might cause these asymmetrical expression profiles [57]. Furthermore, a putative bHLH
transcription factor was identified, named as DcHLH1. DcHLH1 could activate the transcription of
DcF3'H via binding and regulate the promoter activities of DcF3'H, as shown by yeast one-hybrid
screening [58]. F3H (flavonoid 3′-hydroxylase) can catalyze B-ring hydroxylation of flavonoid
derivatives at the 3’-position, forming 3′-hydroxylated flavonoids [59,60].
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A model for dragon’s blood accumulation in D. cambodiana was proposed based on our
transcriptome analysis and previous studies in the model plants [61,62]. It assumes that different
environmental factors can induce transient fluctuations in cytosolic Ca2+ levels in the cell of D.
cambodiana, interact with Ca2+ sensors and resulting in calcium signaling, which always involves
transcription factors or upstream kinases, such as, for instance, calcineurin B-like proteins (CBLs),
Ca2+-dependent protein kinases (CDPKs), and calmodulin-like proteins (CMLs). These sensors
subsequently activate or restrain transcriptional activators, such as promoters, enhancers, or
suppressors, resulting in the activation of the phenylpropane pathway and suppression of the terpene
biosynthetic route. Subsequently, the main chemical constituents of dragons blood are transferred
out of intracellular space to respond to various stimuli through three potential transport mechanisms,
including vesicle trafficking-mediated transport, GST transport, or membrane transport. Our present
and future studies will focus on verifying this hypothesis via multiple approaches involving omics
and genome editing techniques.
6. The Genome Structure Characterization of Dracaena cambodiana
6.1. Organelle Genome Sequencing of Dracaena cambodiana
Plants generally harbor two independent organellar genomes (chloroplast and mitochondria)
that provide invaluable resources for a range of functional, evolutionary, and comparative genomic
studies [63]. The D. cambodiana chloroplast (cp) genome can be found in GenBank, with accession
number MH293451 [64]. The D. cambodiana plant harbors a circular cp genome 156,697 bp in length.
It exhibits the typical plant cp genome structure, including two inverted repeat regions (IRs, 26,526
bp) that are separated by a large single-copy region (LSC, 84,988 bp) and a small single-copy region
(SSC, 18,657 bp). The base composition of the D. cambodiana cp genome was uneven (30.81% A, 18.46%
G, 19.14% C, 31.59% T), with an overall GC content of 37.6%, while those of the IRs, LSC, and SSC
regions were 43%, 35.7%, and 31.1%, respectively. The phylogenetic analyses of entire cp genomes
indicated that D. cambodiana might be classified into the Asparagaceae family, and this result was
supported by the taxonomy systems of National Center for Biotechnology Information (NCBI) and
Angiosperm Phylogeny Group (APG) IV [65]. The cp genome sequence of D. cambodiana encodes a
total of 113 cp genes, 76 of which are protein-coding genes, while four are rRNA genes, 30 are tRNA
genes, and three (infA, matK, and ndhf) are pseudogenes. Of these genes, atpF, ndhA, ndhB, petB, petD,
rpl2, rpl16, rps16, rpoC1, trnA, trnG, trnL, trnK, and trnV contained a single intron while clpP, rps12,
and ycf3 have two introns. These intron sequences might contribute to special DNA barcoding
development and species identification with an internal transcribed spacer from rDNA, especially
ITS2 (Internal Transcribed Spacer 2) [66]. Moreover, the cp genome of D. cochinchinensis was also
released [67]. However, until now, none of the mitochondrial genomes of the Dracaena species have
been characterized.
6.2. Genome SurveySsequencing of Dracaena cambodiana
The genome of D. cambodiana was surveyed with next-generation sequencing on an Illumina
HiSeq 2500 platform, and then the data were assembled and roughly annotated, for obtaining a
comprehensive understanding of the genetic characterization of Dracaena species and the formation
of dragon’s blood. The genome size of D. cambodiana was estimated as 1.12 Gb by k-mer analysis, and
its assembly was considered to be straightforward with the PacBio platform, due to the 53.45% of
repetitive sequences and 0.38% heterozygosis from k-mer distribution. After assembly with
SOAPdevnovo [68], the N50s of the contigs and scaffolds were found to be 1.87 and 3.19 kb. The
longest scaffold was 348.12 kb. Although this is neither the draft nor final genome of D. cambodiana,
it is the first report of the genome-wide characterization of a Dracaena species [69]. The LTR_FINFER
[70] indicated that 30.15% (80,584) scaffolds contained a total of 26,246 simple sequence repeats (SSRs)
and the mono-, di-, and tri- nucleotides comprised nearly 98% of the SSRs. The SciRoKo software [71]
identified 208 types motif and the most abundant motif was hexanucleotide repeats (106 types). The
comprehensive analysis of TRE and RepeatMasker [72] indicated that there were 37.11%
Forests 2020, 11, 372 8 of 13
retroelements and 2.85% DNA transposons in this D. cambodiana genome, with a total of 39.96% TEs
(transposable elements).
The annotation with GeneID [73] predicted 53,700 protein-encoding genes in the survey genome
of D. cambodiana and 38,162 (71.07%) genes could be mapped in the public database, such as Swiss-
Prot, Pfam, GO, KEGG, KOG, COG, TrEMBL, Nt, or Nr [74–78]. Elaeis guineensis, Phoenix dactylifera,
and Musa acuminate were the top three hits for species distribution homologues with D. cambodiana
when surveying genome annotation in the Nr database, for which the percentages were 27.80%
(6,202), 24.76% (5,524), and 11.45% (2,555), respectively. A total of 38,162 predicated genes were
clustered for the gene family analysis with Dendrobium officinale, Asparagus officinalis, Populus
euphratica, and Arabidopsis thaliana as the model plant. The results showed that 5375 gene families
were mutual among five plant species and 1139 gene families were unique to D. cambodiana.
Interestingly, of the 12,510 genes that matched within Gene Ontology [75], 2,620 genes (20%)
were classified into response to stimulus, after cellular process, and metabolic process in the
biological process. This revelation suggested that the plant defense system might be involved in
dragon’s blood formation and the hypothesis was then supported by KEGG mapping [74] and the
transcriptome [44]. Of these 38,162 annotated genes, 9258 could be mapped to 125 KEGG pathways
and 355 (3.83%) genes corresponded to environment information processing, which was much more
than the 267 (2.89%) genes that were mapped to the secondary metabolite biosynthesis and the 184
(1.99%) genes that were involved in terpenoid and polyketide metabolism. Furthermore, this
hypothesis guided us to reanalyze the transcriptome of dragons blood formation by screening the
expression profiles of genes that were related to plant defense.
Plant cell walls, antioxidants, and immune systems all contribute to plant defense [79–82]. For
the plant’s physical defense, cellulose, chitin, pectin, and polysaccharides are the main structural
components of plant cell walls [83]. We detected 41 DEGs that were involved in component synthesis
of plant cell walls, including 25 DEGs encoding pectin esterase, 11 DEGs encoding chitinase, four
DEGs encoding pectate lyase, one DEG encoding cellulase, and four DEGs encoding galactosidase.
The physiological and biochemical barriers of plant defense include antioxidants and immune
systems [81,82]. As an antioxidant, ROS is not only a defense substance, but also an important
signaling factor in plants, and it might be linked to hormone signaling, programmed cell death, and
systemic acquired resistance [82]. However, ROS can also injure normal plant cells, so the plant
antioxidant system will be primed during a ROS burst to protect healthy cells from redundant ROS
[84]. A total of 79 DEGs that were related to the plant antioxidant system were screened in the RNA-
seq data, comprising eight DEGs encoding ascorbate peroxidase, 10 DEGs encoding glutathione s-
transferase, two DEGs encoding superoxide dismutase, 22 DEGs that were involved in peroxidase
synthase, 12 DEGs encoding permease, 21 DEGs related to plant P450 system, and one DEG for
synthesis of each antioxidant, such as glutathione, phytoene, or naringenin. As important defense
components, JA, SA, proline, and trehalose regulate plant defense via signaling transduction or
relieving osmotic stress [85]. We detected seven DEGs encoding lipoxygenase (LOX), two DEGs
encoding allene oxide synthase (AOS), one gene encoding allene oxide cyclase (AOC), and six DEGs
encoding 12-Oxo-phytodienoic acid-10,11-reductase (OPAR) in the JA pathway, which are all
encoding key enzymes in JA biosynthesis [86]. The SA-related DEGs included one PAL
(phenylalanine ammonia lyase), three PBS3 (acyl-adenylate/thioester-forming enzyme), one ICS
(isochorismate synthase), and six EPS1 (enhanced pseudomonas susceptibility 1) [87]. In addition,
three PDG (proline dehydrogenase) and seven TPS (trehalose phosphate synthase) genes might
participate in the formation of dragons blood via osmotic stress.
These initial results that are based on a genomic survey of D. cambodiana are expected to
contribute to further progress in the form of genome sequencing and genetic studies of D. cambodiana
and may improve our understanding of the mechanisms of dragon’s blood formation. However, most
importantly, these results demonstrate effective strategies that involve sequencing platforms and
assembly software selection for the construction of the final chromosome-scale genome of D.
cambodiana.
Forests 2020, 11, 372 9 of 13
7. Conclusions
This paper presents a summary review of the applied history and current progress in
understanding the mechanisms of dragon’s blood formation based on Dracaena cambodiana in China.
Hence, when considering the vulnerable and severely endangered status of the Dracaena tree
population that results from the heavy exploitation of its stem for the production of dragon’s blood,
the research into artificial induction technologies and dragon’s blood formation mechanisms based
on D. cambodiana in China, as presented here, will provide valuable information to aid in the global
conservation of the wild Dracaena species and contribute to understanding the mechanisms of
dragon’s blood formation, eventually helping us to understand the evolution of flavonoid genes and
Dracaena plants.
Author Contributions: Conceptualization, X.D. and W.M.; WritingOriginal Draft Preparation &
Editing, X.D.; WritingReview & Editing, W.M., J.Z., H.W. and H.C.; Funding acquisition, X.D. and
W.M. All authors have read and agreed to the published version of the manuscript.
Funding: This research was supported by the Key Research and Development Project of Hainan
Province (ZDYF2018226 for X.D.), the National Natural Science Foundation of China (81803663 for
X.D.) and the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy
of Tropical Agricultural Sciences (1630052020003 for X.D. and 17CXTD-15 for W.M.).
Acknowledgment: We would like to acknowledge the invitation from 1st World Conference Focused
on Dragon Trees and Petr Maděra who suggested our topic in the content of special issue Dragon
Trees - Tertiary Relicts in Current Reality” in Forests.
Conflicts of Interest: The authors declare no conflicts of interest.
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... Flavonoids and steroidal saponins are vital biological components and the main contributors to the medicative function in dragon's blood [15][16][17][18]. More specifically, flavonoids present antioxidant, anti-inflammatory, antibacterial, and antidiabetic activities, and have favorable biochemical effects on cardiovascular disease and atherosclerosis [19,20]. ...
... Dracaena species are used for dragon's blood production, and usually grow in subtropical and tropical regions [15,44]. Dracaena cambodiana is the main plant resource for dragon's blood production in Southeast Asia and China and can usually be found in barren land, for instance, on cliffs, in stone cracks, or on desert islands [17]. Under these special ecological environments, D. cambodiana experiences periodic exposure to high salinity, high temperature, and high ultraviolet-B radiation. ...
... , including UV protection (GO: 0009650,3), anthocyanin accumulation in tissues in response to UV light (GO: 0043484,17), cellular response to UV-B (GO: 0071493, 1), response to UV (GO: 0009411, 9), response to UV-B (GO: 0010224,22), and response to UV-C (GO: 0010225, 4). A total of 22 unigenes presented potential functions of response to UV-B. ...
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Ultraviolet-B (UV-B) radiation is a major abiotic stress that dragon trees are exposed to during their growth and development; however, it is also an environmental signal perceived by plants that affects the flavonoid pathway. Previous studies have demonstrated that amounts of flavonoids are contained in dragon tree resin, otherwise known as dragon’s blood. However, the traits and mechanism involved in the UV-B-mediated increase in flavonoids in dragon trees are still unknown. Here, we studied the response of Dracaena cambodiana under full solar UV-B radiation. The results showed that the contents of total flavonoids in D. cambodiana significantly increased after UV-B radiation exposure. Then, the transcriptome was used for determining the interactive mechanism of flavonoid accumulation and UV-B stress. Differential expression analyses identified 34 differentially expressed genes (DEGs) involved in flavonoid synthesis; specifically, 24 of the identified DEGs were significantly up-regulated after UV-B radiation exposure. In addition, 57 DEGs involved in Ca2+/kinase sensors, 58 DEGs involved in ROS scavenging and the plant hormone pathway, and 116 DEGs transcription factors in 5 families were further identified and analyzed. Finally, we deduced the potential mechanism of UV-B-promoting flavonoid formation to neutralize ROS damage derived from UV-B radiation in D. cambodiana based on the gene co-expression network and previous studies from other plants. Considering that wild dragon tree populations are currently highly threatened by anthropogenic and natural stressors, the interactive studies between D. cambodiana plants and UV-B radiation provide valuable information toward understanding the mechanism of dragon’s blood formation and help us reveal the evolution of D. cambodiana, with the eventual goal of aiding in the global conservation of this precious biological resource.
... There is much confusion surrounding the origin and identity of dragon's blood trees, and many plants can produce dragon's blood, including Croton, Dracaena, Daemonorops, and Pterocarpus (Gupta et al., 2008;Ding et al., 2020). In the wild, Dracaena grows slowly, and only trees that are over 30 years of age have been shown to produce dragon's blood (Edwards et al., 1997;Wang et al., 2013;Ding et al., 2020). ...
... There is much confusion surrounding the origin and identity of dragon's blood trees, and many plants can produce dragon's blood, including Croton, Dracaena, Daemonorops, and Pterocarpus (Gupta et al., 2008;Ding et al., 2020). In the wild, Dracaena grows slowly, and only trees that are over 30 years of age have been shown to produce dragon's blood (Edwards et al., 1997;Wang et al., 2013;Ding et al., 2020). Due to continuous overexploitation, Dracaena are now threatened with depletion. ...
... Thus far, hundreds of flavonoids have been isolated from the resin wood of D. cochinchinensis (for reviews, see Gupta et al., 2008;Fan et al., 2014, Sun et al., 2019. In addition, terpenoids and steroidal saponins are also effective components of dragon's blood (Fan et al., 2014;Teng et al., 2015;Mad era et al., 2020;Vanickova et al., 2020); these components have been reported to be cytotoxic and could be used as anticarcinogens (Darias et al., 1989;Mimaki et al., 1999;Gon zalez et al., 2003;Her nandez et al., 2004;Ding et al., 2020;Mad era et al., 2020). ...
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Dracaena, a remarkably long-lived and slowly maturing species of plant, is world famous for its ability to produce dragon’s blood, a precious traditional medicine used by different cultures since ancient times. However, there is no detailed and high-quality genome available for this species at present; thus, the molecular mechanisms underlying the important traits of this plant are largely unknown. These factors seriously limit the protection and regeneration of the rare and endangered plant resources. Here, we sequenced and assembled the genome of Dracaena cochinchinensis at the chromosomal level. The D. cochinchinensis genome covered 1.21 Gb with a scaffold N50 of 50.06 Mb and encodes 31,619 predicted protein-coding genes. Analysis showed that D. cochinchinensis has undergone two whole genome duplications (WGDs) and two long terminal repeat (LTR) insertion burst events. The expansion of two genes, cis-zeatin O-glucosyltransferase (cZOGT) and small auxin up-regulated RNA (SAUR), were found to account for its longevity and slow growth. Two transcription factors (bHLH and MYB) were found to be core regulators of the flavonoid biosynthesis pathway and reactive oxygen species (ROS) was identified as the specific signal molecule responsible for the injury-induced formation of dragon’s blood. Our study provides high-quality genomic knowledge relating to D. cochinchinensis and significant insight into the molecular mechanisms responsible for longevity and the formation of dragon’s blood. These findings will facilitate the resource protection and sustainable utilization of Dracaena.
... It is not clear how the resin is formed in parenchyma cells and transported along the tissue. Ding et al. (2020) have suggested that the major chemical constituents of dragon's blood are transported out of the intracellular space in response to various stimuli by three potential transport mechanisms i.e., vesicle trafficking mediated transport, GST (glutathione S-transferase) transport or membrane transport. Recent studies of the leaf shedding of dragon trees have shown that dragon's blood is in the form of vesicles, which have a tendency to aggregate and fill the cells or intercellular spaces (Jura-Morawiec et al., 2023). ...
... After the injury of the secondary tissues, resin typically fills the parenchyma cells and enters tracheids through the pits occluding their lumen (Cui et al., 2013;Xu et al., 2022; Figure 1B). Resin secretion can be additionally enhanced by high humidity and fungal infection (Wang et al., 2010(Wang et al., , 2011, and its accumulation increases after acid and sodium salt treatment (reviewed by Ding et al., 2020). ...
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The evolution of the vascular system has led to the formation of conducting and supporting elements and those that are involved in the mechanisms of storage and defense against the influence of biotic and abiotic factors. In the case of the latter, the general evolutionary trend was probably related to a change in their arrangement, i.e. from cells scattered throughout the tissue to cells organized into ducts or cavities. These cells, regardless of whether they occur alone or in a cellular structure, are an important defense element of trees, having the ability to synthesize, among others, natural resins. In the tracheid-based secondary xylem of gymnosperms, the resin ducts, which consist of secretory cells, are of two types: axial, interspersed between the tracheids, and radial, carried in some rays. They are interconnected and form a continuous system. On the other hand, in the tracheid-based secondary xylem of monocotyledons, the resin-producing secretory cells do not form specialized structures. This review summarizes knowledge on the morpho-anatomical features of various types of resin-releasing secretory cells in relation to their: (i) location, (ii) origin, (iii) mechanism of formation, (iv) and ecological significance.
... In general, dragon's blood is produced from the trunk of wild-grown Dracaena trees more than 30-50 years of age, and which can be even thousands of years old. Production of dragon's blood in the trunk is induced by stress under natural conditions (Ding et al., 2020;Maděra et al., 2020). ...
... Many studies shown that various biotic or abiotic stresses can induce the Dracaena tree to produce dragon's blood (Ding et al., 2020;Zhu et al., 2020). The Dracaena tree has no special tissue to secrete and store dragon's blood, which is synthesized and accumulated when the plant is stressed (Cui et al., 2013). ...
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Background Dragon′s blood is a well-known traditional Chinese medicine, isolated mainly from the trunks of Dracaena trees. Overexploitation of the Dracaena resource has resulted in this plant becoming endangered. This work aimed to assess the potential to use various tissues of the Dracaena cochinchinensis tree, such as leaves and roots, to produce dragon′s blood. Results We found that many dragon′s blood components accumulated in the bark of trunk and roots of D. cochinchinensis under natural conditions. In addition, small amounts of loureirin A were detected in leaves, suggesting that the leaves could be a potential source of dragon′s blood. Real-time quantitative PCR analysis showed that most of the genes tested in this study, which encoded enzymes involved in the biosynthesis of dragon′s blood flavonoids, were highly expressed in the bark of roots and trunk. Conclusion We confirmed that the bark of roots and trunk of D. cochinchinensis tree were the main tissues for the synthesis and storage of dragon′s blood under natural conditions. This study demonstrated the potential to extract dragon′s blood from the roots that have been abandoned due to mining difficulties without destroying the tree, a process which would be beneficial to the protection of the endangered wild D. cochinchinensis tree populations. Keywords: Dracaena tree ; Dragon′s blood accumulation site; Gene expression; Loureirin A; Loureirin B
... Various cultures have used dragon's blood in traditional medicine, including the ancient Chinese, Greek, and Roman civilizations. It continues to be of interest in modern research for its potential therapeutic applications [35]. However, although 4-hydroxychalcones are widely represented in this biological material, they occur in mixtures that make their isolation and purification very difficult. ...
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The quest for novel therapeutic agents has rekindled interest in natural products, particularly those derived from biotransformation processes. Dihydrochalcones, a class of plant secondary metabolites, exhibit a range of pharmacological properties. Chalcone and dihydrochalcone compounds with the characteristic 4’-hydroxy substitution are present in ‘dragon’s blood’ resin, known for its traditional medicinal uses and complex composition, making the isolation of these compounds challenging. This study investigates the efficient production of 4′-hydroxydihydrochalcones using non-conventional yeast strains. We evaluated the biotransformation efficiency of various 4′-hydroxychalcone substrates utilizing yeast strains such as Yarrowia lipolytica KCh 71, Saccharomyces cerevisiae KCh 464, Rhodotorula rubra KCh 4 and KCh 82, and Rhodotorula glutinis KCh 242. Our findings revealed that Yarrowia lipolytica KCh 71, Rhodotorula rubra KCh 4 and KCh 82, and Rhodotorula glutinis KCh 242 exhibited the highest conversion efficiencies, exceeding 98% within one hour for most substrates. The position of methoxy substituents in the chalcone ring significantly influenced hydrogenation efficiency. Moreover, we observed isomerization of trans-4′-hydroxy-2-methoxychalcone to its cis isomer, catalyzed by light exposure. This study underscores the potential of using yeast strains for the sustainable and efficient production of dihydrochalcones, providing a foundation for developing new therapeutic agents and nutraceuticals.
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[Free PDF download website: https://www.sciencedirect.com/science/article/pii/S1021949818301492?via%3Dihub] Dragon blood has been used in wound treatment for many years and can be obtained from several distinct plant species. Dracorhodin, the active substituent of dragon blood, is a characteristic compound of the palm tree, Daemonorops draco. At present, the only method to evaluate the quality of commercial dragon blood samples is a HPLC method which determines the amount of dracorhodin in a dragon blood sample. In this study, we used zebrafish embryos as a platform to demonstrate the in vivo pro-angiogenic activity of dracorhodin perchlorate, the chemically synthesized analog of dracorhodin. By using this platform, three different commercial dragon blood samples were also examined. Our results clearly show that even though the commercial dragon blood samples had similar amounts of dracorhodin, they showed highly variable biological activity, such as pro-angiogenic effects and toxicity. In short, an in vivo activity assay platform for rapidly examining the biological activity of commercial dragon blood samples was successfully established here, which complements the current HPLC-based assay method.
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Dracaena cambodiana is a traditional medicinal plant used for producing dragon’s blood. The plants and dragon’s blood of D. cambodiana contain a rich variety of steroidal saponins. However, little is known about steroidal saponin biosynthesis and its regulation in D. cambodiana. Here, 122 genes encoding enzymes involved in steroidal saponin biosynthesis were identified based on transcriptome data, with 29 of them containing complete open reading frames (ORF). Transcript expression analysis revealed that several genes related to steroidal saponin biosynthesis showed distinct tissue-specific expression patterns; the expression levels of genes encoding the key enzymes involved in the biosynthesis and early modification of steroidal saponins were significantly down-regulated in the stems in response to the inducer of dragon’s blood, exhibiting positive correlations with the content of steroidal saponins. These results provide insights on the steroidal saponins biosynthetic pathway and mechanisms underlying induced formation of dragon’s blood in D. cambodiana.