Observation the responses of Escherichia coli strains with multiple drug resistance and overexpressed efflux pumps against efflux pump inhibitors

Abstract

The aim of this study is firstly of determine the efflux pump expressions of E. coli strains presenting multi drug resistance by using ethidium bromide and secondly to observe the responses of these E. coli strains against efflux pump inhibitors by using a combination of an antibiotic and an inhibitor. Antibiotics are compounds that can stop or kill the viability of microorganisms, but for some reason, microorganisms in time show resistance to antibiotics. One of these reasons for developing resistance to antibiotics is efflux pumps. By means of these pumps, the antibiotic entered into the bacterial cell is pumped out; therefore, it cannot act against the bacteria. This situation increases the antibiotic resistance. Thus, microorganisms will develop multiple drug resistance against not only the antibiotic used, but also for many other antibiotics and they rapidly spread to the environment. For this reason, antibiotic resistance has become a major problem all over the world, and new antibiotics and efflux pump inhibitor studies, which can affect these microorganisms that are resistant to multiple drugs are continuing rapidly. According to the results of this study; effect of verapamil and thioridazine was observed to be more effective than PAβN. As a result 98.41% of the E. coli strains used in the study was found to be affected by verapamil, where 96.82% by thioridazine and only 38.09% was affected by PAβN inhibitor.

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3rd International Eurasian Conference on
Biological and Chemical Sciences
(EurasianBioChem 2020)
19-20 March 2020, Ankara, Turkey
www. EurasianBioChem.org
➢ ORAL PRESENTATION
89
Observation the responses of Escherichia coli strains with multiple drug resistance and overexpressed efflux pumps
against efflux pump inhibitors
Eda Altınöz*, Ergin Murat Altuner
Kastamonu University, Faculty of Science and Arts, Department of Biology, Kastamonu, Turkey.
*Corresponding author e-mail: altinozedaa@gmail.com
Abstract
The aim of this study is firstly of determine the efflux pump expressions of E. coli strains presenting multi drug resistance
by using ethidium bromide and secondly to observe the responses of these E. coli strains against efflux pump inhibitors by
using a combination of an antibiotic and an inhibitor. Antibiotics are compounds that can stop or kill the viability of
microorganisms, but for some reason, microorganisms in time show resistance to antibiotics. One of these reasons for
developing resistance to antibiotics is efflux pumps. By means of these pumps, the antibiotic entered into the bacterial cell is
pumped out; therefore, it cannot act against the bacteria. This situation increases the antibiotic resistance. Thus,
microorganisms will develop multiple drug resistance against not only the antibiotic used, but also for many other
antibiotics and they rapidly spread to the environment. For this reason, antibiotic resistance has become a major problem all
over the world, and new antibiotics and efflux pump inhibitor studies, which can affect these microorganisms that are
resistant to multiple drugs are continuing rapidly. According to the results of this study; effect of verapamil and thioridazine
was observed to be more effective than PAβN. As a result 98.41% of the E. coli strains used in the study was found to be
affected by verapamil, where 96.82% by thioridazine and only 38.09% was affected by PAβN inhibitor.
Keywords: Multiple drug resistance, antibiotics, efflux pumps, efflux pump inhibitors, E.coli.